Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sn1-specific diacylglycerol lipase alpha

Gene

Dagla

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses (By similarity).By similarity

Cofactori

Ca2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei472 – 4721Charge relay systemPROSITE-ProRule annotation
Active sitei524 – 5241Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-426048. Arachidonate production from DAG.

Protein family/group databases

ESTHERiratno-q5ylm1. Lipase_3.

Names & Taxonomyi

Protein namesi
Recommended name:
Sn1-specific diacylglycerol lipase alpha (EC:3.1.1.-)
Short name:
DGL-alpha
Alternative name(s):
Neural stem cell-derived dendrite regulator
Gene namesi
Name:Dagla
Synonyms:Nsddr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1359461. Dagla.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2222CytoplasmicSequence analysisAdd
BLAST
Transmembranei23 – 4321HelicalSequence analysisAdd
BLAST
Topological domaini44 – 6017ExtracellularSequence analysisAdd
BLAST
Transmembranei61 – 8121HelicalSequence analysisAdd
BLAST
Topological domaini82 – 10120CytoplasmicSequence analysisAdd
BLAST
Transmembranei102 – 12221HelicalSequence analysisAdd
BLAST
Topological domaini123 – 13614ExtracellularSequence analysisAdd
BLAST
Transmembranei137 – 15721HelicalSequence analysisAdd
BLAST
Topological domaini158 – 1044887CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10441044Sn1-specific diacylglycerol lipase alphaPRO_0000248349Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi133 – 1331N-linked (GlcNAc...)Sequence analysis
Modified residuei728 – 7281PhosphoserineCombined sources
Modified residuei730 – 7301PhosphoserineBy similarity
Modified residuei733 – 7331PhosphoserineCombined sources
Modified residuei744 – 7441PhosphoserineCombined sources
Modified residuei784 – 7841PhosphoserineCombined sources
Modified residuei786 – 7861PhosphoserineCombined sources
Modified residuei808 – 8081PhosphoserineCombined sources
Modified residuei835 – 8351PhosphoserineCombined sources
Modified residuei849 – 8491PhosphoserineCombined sources
Modified residuei954 – 9541PhosphoserineBy similarity
Modified residuei1025 – 10251PhosphothreonineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ5YLM1.
PRIDEiQ5YLM1.

PTM databases

iPTMnetiQ5YLM1.
PhosphoSiteiQ5YLM1.
SwissPalmiQ5YLM1.

Expressioni

Gene expression databases

BgeeiENSRNOG00000027264.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000033416.

Structurei

3D structure databases

ProteinModelPortaliQ5YLM1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi315 – 34935Cys-richAdd
BLAST

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2088. Eukaryota.
ENOG410XRFI. LUCA.
GeneTreeiENSGT00530000063682.
HOGENOMiHOG000015738.
HOVERGENiHBG081435.
InParanoidiQ5YLM1.
KOiK13806.
OMAiEPRESMQ.
OrthoDBiEOG091G04AM.
PhylomeDBiQ5YLM1.
TreeFamiTF312928.

Family and domain databases

Gene3Di3.40.50.1820. 3 hits.
InterProiIPR029058. AB_hydrolase.
IPR002921. Fungal_lipase-like.
[Graphical view]
PfamiPF01764. Lipase_3. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5YLM1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGIVVFRRR WSVGSDDLVL PAIFLFLLHT TWFVILSVVL FGLVYNPHEA
60 70 80 90 100
CSLNLVDHGR GYLGILLSCM IAEMAIIWLS MRGGILYTEP RDSMQYVLYV
110 120 130 140 150
RLAILVIEFI YAIVGIVWLT QYYTSCNDLT AKNVTLGMVV CNWVVILSVC
160 170 180 190 200
ITVLCVFDPT GRTFVKLRAT KRRQRNLRTY NLRHRLEEGQ ATSWSRRLKV
210 220 230 240 250
FLCCTRTKDS QSDAYSEIAY LFAEFFRDLD IVPSDIIAGL VLLRQRQRAK
260 270 280 290 300
RNAVLDEANN DILAFLSGMP VTRNTKYLDL KNSHEMLRYK EVCYYMLFAL
310 320 330 340 350
AAYGWPMYLM RKPTCGLCQL ARSCSCCLCP ARPRFAPGVT IEEDNCCGCN
360 370 380 390 400
AIAIRRHFLD ENMTAVDIVY TSCHDAVYET PFYVAVDHDK KKVVISIRGT
410 420 430 440 450
LSPKDALTDL TGDAERLPVE GHRGTWLGHK GMVLSAEYIK KKLEQEMVLS
460 470 480 490 500
QAFGRDLGRG TKHYGLIVVG HSLGAGTAAI LSFLLRPQYP TLKCFAYSPP
510 520 530 540 550
GGLLSEDAME YSKEFVTAVV LGKDLVPRIG LSQLEGFRRQ LLDVLQRSTK
560 570 580 590 600
PKWRIIVGAT KCIPKSELPE DQVEVTALAS TRLWTHPSDL TIALSASTPL
610 620 630 640 650
YPPGRIIHVV HNHPAEQCCC CEQEEPTYFA IWGDNKAFNE VIISPAMLHE
660 670 680 690 700
HLPYVVMEGL NKVLENYNKG KTALLSAAKV MVSPTEVDLT PELIFQQQPL
710 720 730 740 750
PTGPPLPTGL ALELPATEHR NSSVRSKSQS EMSLEGFSEG RLLSPVAAAS
760 770 780 790 800
AARQDPVELL LLSTQERLAA ELQSRRAPLA TMESLSDTES LYSFDSRRSS
810 820 830 840 850
GFRSIRGSPS LHAVLERDEG HLFYIDPAIP EENPSLSSRT ELLAADSLSK
860 870 880 890 900
HSQDTQPLEA ALGSGGVTPE RPPSAANDEE EAAGGSEGGG VAPRGELALH
910 920 930 940 950
NGRLGDSPSP QVLEFAEFID SLFNLDSKSS SFQDLYCMMV PESPTSDYTE
960 970 980 990 1000
GPKSPSQQEI LLRAQFEPNL VPKPPRLFAG SAEPSSGISL SPSFPLSSSG
1010 1020 1030 1040
ELMDLTPTGL SSQECLATDK IRTSTPTGHG ASPTKQDDLV ISAR
Length:1,044
Mass (Da):115,301
Last modified:November 23, 2004 - v1
Checksum:i513F144EFF7E995A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY275375 mRNA. Translation: AAQ17117.1.
RefSeqiNP_001005886.1. NM_001005886.1.
XP_008758451.1. XM_008760229.1.
XP_008758452.1. XM_008760230.1.
UniGeneiRn.124941.

Genome annotation databases

EnsembliENSRNOT00000037624; ENSRNOP00000033416; ENSRNOG00000027264.
GeneIDi309207.
KEGGirno:309207.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY275375 mRNA. Translation: AAQ17117.1.
RefSeqiNP_001005886.1. NM_001005886.1.
XP_008758451.1. XM_008760229.1.
XP_008758452.1. XM_008760230.1.
UniGeneiRn.124941.

3D structure databases

ProteinModelPortaliQ5YLM1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000033416.

Protein family/group databases

ESTHERiratno-q5ylm1. Lipase_3.

PTM databases

iPTMnetiQ5YLM1.
PhosphoSiteiQ5YLM1.
SwissPalmiQ5YLM1.

Proteomic databases

PaxDbiQ5YLM1.
PRIDEiQ5YLM1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000037624; ENSRNOP00000033416; ENSRNOG00000027264.
GeneIDi309207.
KEGGirno:309207.

Organism-specific databases

CTDi747.
RGDi1359461. Dagla.

Phylogenomic databases

eggNOGiKOG2088. Eukaryota.
ENOG410XRFI. LUCA.
GeneTreeiENSGT00530000063682.
HOGENOMiHOG000015738.
HOVERGENiHBG081435.
InParanoidiQ5YLM1.
KOiK13806.
OMAiEPRESMQ.
OrthoDBiEOG091G04AM.
PhylomeDBiQ5YLM1.
TreeFamiTF312928.

Enzyme and pathway databases

ReactomeiR-RNO-426048. Arachidonate production from DAG.

Miscellaneous databases

PROiQ5YLM1.

Gene expression databases

BgeeiENSRNOG00000027264.

Family and domain databases

Gene3Di3.40.50.1820. 3 hits.
InterProiIPR029058. AB_hydrolase.
IPR002921. Fungal_lipase-like.
[Graphical view]
PfamiPF01764. Lipase_3. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDGLA_RAT
AccessioniPrimary (citable) accession number: Q5YLM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: November 23, 2004
Last modified: September 7, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.