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Q5YLG1

- GUNA_BACPU

UniProt

Q5YLG1 - GUNA_BACPU

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Protein

Endoglucanase A

Gene
eglA
Organism
Bacillus pumilus (Bacillus mesentericus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Active on carboxymethyl cellulose and carboxymethyl cellulose-RBB but not avicel, xanthan gum, carboxymethyl-curdulan-RBB or carboxymethyl-xylan-RBB.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.1 Publication

Enzyme regulationi

Strongly inhibited by ZnCl2 and by EDTA.1 Publication

pH dependencei

Optimum pH is 5-8.1 Publication

Temperature dependencei

Optimum temperature is 60 degrees Celsius. Retains more than 90% activity after 24 hours at 50 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei419 – 4191 By similarity
Active sitei457 – 4571 By similarity
Active sitei466 – 4661 By similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC
  2. cellulose binding Source: InterPro

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM3. Carbohydrate-Binding Module Family 3.
GH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase A (EC:3.2.1.4)
Alternative name(s):
Endo-1,4-beta-glucanase A
Gene namesi
Name:eglA
OrganismiBacillus pumilus (Bacillus mesentericus)
Taxonomic identifieri1408 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 659659Endoglucanase APRO_5000091857Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ5YLG1.
SMRiQ5YLG1. Positions 45-657.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini501 – 658158CBM3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 500500CatalyticAdd
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
2.60.40.710. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF00942. CBM_3. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SMARTiSM01067. CBM_3. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF49384. SSF49384. 1 hit.
PROSITEiPS51172. CBM3. 1 hit.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5YLG1-1 [UniParc]FASTAAdd to Basket

« Hide

MLIFETYLIL FKTVQITKRR IERRRLRLLN QCFTKKEGVS NREMASYNYV    50
EVLQKSMLFY EAQRSGRLPE SNRLNWRGDS GLKDGKDVGH DLTGGWYDAG 100
DHVKFGLPMA YSAAVLAWTV YEYREAYEEA ELLDEILDQI KWATDYFLKA 150
HTGPNEFWAQ VGDGNADHAW WGPAEVMPMN RPAFKIDEHC PGTEVAAQTA 200
AALAAGSIIF KETDASYAAK LLTHAKQLYA FADRYRGKYT DCVTNAQPFY 250
NSWSGYVDEL IWGGIWLYLA TNEETYLNKA LKAVEEWPQD WDYTFTMSWD 300
NTFFASQILL ARITKENRFI ESTERNLDYW TTGLVQNGKV ERITYTPGGL 350
AWLDQWGSLR YAANAAFLAF VYADWVSDQE KKNRYQSFAI KQTHYMLGDN 400
PLNRSYVVGF GQNSPKHPHH RTAHGSWSNQ LTNPPSHRHT LYGALVGGPN 450
AQDQYDDDIS DYISNEVATD YNAAFTGNIA KMVQLFGEGQ SKLPNFPPKE 500
QVEDEFFVEA AVMHNDTTST QVKAVLYNRS GWPARSSQTL SFRYYVNLSE 550
VFAKGFTEKD IQVTAAYNEG ASLSPLKVYD ASSRVYFAEI DFTGVVISPR 600
GESEHKKEIQ FRLSAPNGSN IWDASNDYSY QGLTSNMQKT TKIPVFEDGV 650
LVFGTLPDK 659
Length:659
Mass (Da):74,985
Last modified:November 23, 2004 - v1
Checksum:i13DE360B374DA139
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY339624 Genomic DNA. Translation: AAQ91573.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY339624 Genomic DNA. Translation: AAQ91573.1 .

3D structure databases

ProteinModelPortali Q5YLG1.
SMRi Q5YLG1. Positions 45-657.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM3. Carbohydrate-Binding Module Family 3.
GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
2.60.40.710. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view ]
Pfami PF00942. CBM_3. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view ]
SMARTi SM01067. CBM_3. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
SSF49384. SSF49384. 1 hit.
PROSITEi PS51172. CBM3. 1 hit.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: CL16.

Entry informationi

Entry nameiGUNA_BACPU
AccessioniPrimary (citable) accession number: Q5YLG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 23, 2004
Last modified: October 16, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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