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Protein

Endoglucanase A

Gene

eglA

Organism
Bacillus pumilus (Bacillus mesentericus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Active on carboxymethyl cellulose and carboxymethyl cellulose-RBB but not avicel, xanthan gum, carboxymethyl-curdulan-RBB or carboxymethyl-xylan-RBB.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.1 Publication

Enzyme regulationi

Strongly inhibited by ZnCl2 and by EDTA.1 Publication

pH dependencei

Optimum pH is 5-8.1 Publication

Temperature dependencei

Optimum temperature is 60 degrees Celsius. Retains more than 90% activity after 24 hours at 50 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei419By similarity1
Active sitei457By similarity1
Active sitei466By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM3 Carbohydrate-Binding Module Family 3
GH9 Glycoside Hydrolase Family 9

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase A (EC:3.2.1.4)
Alternative name(s):
Endo-1,4-beta-glucanase A
Gene namesi
Name:eglA
OrganismiBacillus pumilus (Bacillus mesentericus)
Taxonomic identifieri1408 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_50000918571 – 659Endoglucanase AAdd BLAST659

Structurei

3D structure databases

ProteinModelPortaliQ5YLG1
SMRiQ5YLG1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini501 – 658CBM3PROSITE-ProRule annotationAdd BLAST158

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 500CatalyticAdd BLAST500

Sequence similaritiesi

Family and domain databases

Gene3Di1.50.10.10, 1 hit
2.60.40.710, 1 hit
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR008965 CBM2/CBM3_carb-bd_dom_sf
IPR001956 CBM3
IPR036966 CBM3_sf
IPR001701 Glyco_hydro_9
IPR033126 Glyco_hydro_9_Asp/Glu_AS
IPR018221 Glyco_hydro_9_His_AS
PfamiView protein in Pfam
PF00942 CBM_3, 1 hit
PF00759 Glyco_hydro_9, 1 hit
SMARTiView protein in SMART
SM01067 CBM_3, 1 hit
SUPFAMiSSF48208 SSF48208, 1 hit
SSF49384 SSF49384, 1 hit
PROSITEiView protein in PROSITE
PS51172 CBM3, 1 hit
PS00592 GLYCOSYL_HYDROL_F9_1, 1 hit
PS00698 GLYCOSYL_HYDROL_F9_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q5YLG1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIFETYLIL FKTVQITKRR IERRRLRLLN QCFTKKEGVS NREMASYNYV
60 70 80 90 100
EVLQKSMLFY EAQRSGRLPE SNRLNWRGDS GLKDGKDVGH DLTGGWYDAG
110 120 130 140 150
DHVKFGLPMA YSAAVLAWTV YEYREAYEEA ELLDEILDQI KWATDYFLKA
160 170 180 190 200
HTGPNEFWAQ VGDGNADHAW WGPAEVMPMN RPAFKIDEHC PGTEVAAQTA
210 220 230 240 250
AALAAGSIIF KETDASYAAK LLTHAKQLYA FADRYRGKYT DCVTNAQPFY
260 270 280 290 300
NSWSGYVDEL IWGGIWLYLA TNEETYLNKA LKAVEEWPQD WDYTFTMSWD
310 320 330 340 350
NTFFASQILL ARITKENRFI ESTERNLDYW TTGLVQNGKV ERITYTPGGL
360 370 380 390 400
AWLDQWGSLR YAANAAFLAF VYADWVSDQE KKNRYQSFAI KQTHYMLGDN
410 420 430 440 450
PLNRSYVVGF GQNSPKHPHH RTAHGSWSNQ LTNPPSHRHT LYGALVGGPN
460 470 480 490 500
AQDQYDDDIS DYISNEVATD YNAAFTGNIA KMVQLFGEGQ SKLPNFPPKE
510 520 530 540 550
QVEDEFFVEA AVMHNDTTST QVKAVLYNRS GWPARSSQTL SFRYYVNLSE
560 570 580 590 600
VFAKGFTEKD IQVTAAYNEG ASLSPLKVYD ASSRVYFAEI DFTGVVISPR
610 620 630 640 650
GESEHKKEIQ FRLSAPNGSN IWDASNDYSY QGLTSNMQKT TKIPVFEDGV

LVFGTLPDK
Length:659
Mass (Da):74,985
Last modified:November 23, 2004 - v1
Checksum:i13DE360B374DA139
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY339624 Genomic DNA Translation: AAQ91573.1

Entry informationi

Entry nameiGUNA_BACPU
AccessioniPrimary (citable) accession number: Q5YLG1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 23, 2004
Last modified: March 28, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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