Q5YLG1 (GUNA_BACPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase A
EC=3.2.1.4

Alternative name(s):
Endo-1,4-beta-glucanase A

Gene names

Name:

eglA

Organism

Bacillus pumilus (Bacillus mesentericus)

Taxonomic identifier

1408 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	659 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Active on carboxymethyl cellulose and carboxymethyl cellulose-RBB but not avicel, xanthan gum, carboxymethyl-curdulan-RBB or carboxymethyl-xylan-RBB. Ref.1
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. Ref.1
Enzyme regulation	Strongly inhibited by ZnCl2 and by EDTA. Ref.1
Subcellular location	Secreted Ref.1.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family. Contains 1 CBM3 (carbohydrate binding type-3) domain.
Biophysicochemical properties	pH dependence: Optimum pH is 5-8. Ref.1 Temperature dependence: Optimum temperature is 60 degrees Celsius. Retains more than 90% activity after 24 hours at 50 degrees Celsius.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC cellulose binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 659	659	Endoglucanase A		PRO_5000091857
Regions
	Domain	501 – 658	158	CBM3
	Region	1 – 500	500	Catalytic
Sites
	Active site	419	1	By similarity
	Active site	457	1	By similarity
	Active site	466	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5YLG1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 13DE360B374DA139	FASTA	659	74,985
	10 20 30 40 50 60 MLIFETYLIL FKTVQITKRR IERRRLRLLN QCFTKKEGVS NREMASYNYV EVLQKSMLFY 70 80 90 100 110 120 EAQRSGRLPE SNRLNWRGDS GLKDGKDVGH DLTGGWYDAG DHVKFGLPMA YSAAVLAWTV 130 140 150 160 170 180 YEYREAYEEA ELLDEILDQI KWATDYFLKA HTGPNEFWAQ VGDGNADHAW WGPAEVMPMN 190 200 210 220 230 240 RPAFKIDEHC PGTEVAAQTA AALAAGSIIF KETDASYAAK LLTHAKQLYA FADRYRGKYT 250 260 270 280 290 300 DCVTNAQPFY NSWSGYVDEL IWGGIWLYLA TNEETYLNKA LKAVEEWPQD WDYTFTMSWD 310 320 330 340 350 360 NTFFASQILL ARITKENRFI ESTERNLDYW TTGLVQNGKV ERITYTPGGL AWLDQWGSLR 370 380 390 400 410 420 YAANAAFLAF VYADWVSDQE KKNRYQSFAI KQTHYMLGDN PLNRSYVVGF GQNSPKHPHH 430 440 450 460 470 480 RTAHGSWSNQ LTNPPSHRHT LYGALVGGPN AQDQYDDDIS DYISNEVATD YNAAFTGNIA 490 500 510 520 530 540 KMVQLFGEGQ SKLPNFPPKE QVEDEFFVEA AVMHNDTTST QVKAVLYNRS GWPARSSQTL 550 560 570 580 590 600 SFRYYVNLSE VFAKGFTEKD IQVTAAYNEG ASLSPLKVYD ASSRVYFAEI DFTGVVISPR 610 620 630 640 650 GESEHKKEIQ FRLSAPNGSN IWDASNDYSY QGLTSNMQKT TKIPVFEDGV LVFGTLPDK

References

[1]

"Molecular characterization of a beta-1,4-endoglucanase from an endophytic Bacillus pumilus strain." Lima A.O.S., Quecine M.C., Fungaro M.H.P., Andreote F.D., Maccheroni W. Jr., Araujo W.L., Silva-Filho M.C., Pizzirani-Kleiner A.A., Azevedo J.L. Appl. Microbiol. Biotechnol. 68:57-65(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: CL16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY339624 Genomic DNA. Translation: AAQ91573.1.
3D structure databases
ProteinModelPortal	Q5YLG1.
SMR	Q5YLG1. Positions 45-657.
ModBase	Search...
Protein family/group databases
CAZy	CBM3. Carbohydrate-Binding Module Family 3. GH9. Glycoside Hydrolase Family 9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.50.10.10. 1 hit. 2.60.40.710. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR008965. Carb-bd_dom. IPR001956. CBD_3. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Pfam	PF00942. CBM_3. 1 hit. PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SMART	SM01067. CBM_3. 1 hit. [Graphical view]
SUPFAM	SSF49384. Cellul_bind. 1 hit. SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS51172. CBM3. 1 hit. PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNA_BACPU

Accession

Primary (citable) accession number: Q5YLG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 23, 2004
Last modified:	May 29, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program