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Q5YLG1 (GUNA_BACPU) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase A

EC=3.2.1.4
Alternative name(s):
Endo-1,4-beta-glucanase A
Gene names
Name:eglA
OrganismBacillus pumilus (Bacillus mesentericus)
Taxonomic identifier1408 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length659 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Active on carboxymethyl cellulose and carboxymethyl cellulose-RBB but not avicel, xanthan gum, carboxymethyl-curdulan-RBB or carboxymethyl-xylan-RBB. Ref.1

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. Ref.1

Enzyme regulation

Strongly inhibited by ZnCl2 and by EDTA. Ref.1

Subcellular location

Secreted Ref.1.

Sequence similarities

Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Contains 1 CBM3 (carbohydrate binding type-3) domain.

Biophysicochemical properties

pH dependence:

Optimum pH is 5-8. Ref.1

Temperature dependence:

Optimum temperature is 60 degrees Celsius. Retains more than 90% activity after 24 hours at 50 degrees Celsius.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   Cellular componentSecreted
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

cellulose binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 659659Endoglucanase A
PRO_5000091857

Regions

Domain501 – 658158CBM3
Region1 – 500500Catalytic

Sites

Active site4191 By similarity
Active site4571 By similarity
Active site4661 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5YLG1 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 13DE360B374DA139

FASTA65974,985
        10         20         30         40         50         60 
MLIFETYLIL FKTVQITKRR IERRRLRLLN QCFTKKEGVS NREMASYNYV EVLQKSMLFY 

        70         80         90        100        110        120 
EAQRSGRLPE SNRLNWRGDS GLKDGKDVGH DLTGGWYDAG DHVKFGLPMA YSAAVLAWTV 

       130        140        150        160        170        180 
YEYREAYEEA ELLDEILDQI KWATDYFLKA HTGPNEFWAQ VGDGNADHAW WGPAEVMPMN 

       190        200        210        220        230        240 
RPAFKIDEHC PGTEVAAQTA AALAAGSIIF KETDASYAAK LLTHAKQLYA FADRYRGKYT 

       250        260        270        280        290        300 
DCVTNAQPFY NSWSGYVDEL IWGGIWLYLA TNEETYLNKA LKAVEEWPQD WDYTFTMSWD 

       310        320        330        340        350        360 
NTFFASQILL ARITKENRFI ESTERNLDYW TTGLVQNGKV ERITYTPGGL AWLDQWGSLR 

       370        380        390        400        410        420 
YAANAAFLAF VYADWVSDQE KKNRYQSFAI KQTHYMLGDN PLNRSYVVGF GQNSPKHPHH 

       430        440        450        460        470        480 
RTAHGSWSNQ LTNPPSHRHT LYGALVGGPN AQDQYDDDIS DYISNEVATD YNAAFTGNIA 

       490        500        510        520        530        540 
KMVQLFGEGQ SKLPNFPPKE QVEDEFFVEA AVMHNDTTST QVKAVLYNRS GWPARSSQTL 

       550        560        570        580        590        600 
SFRYYVNLSE VFAKGFTEKD IQVTAAYNEG ASLSPLKVYD ASSRVYFAEI DFTGVVISPR 

       610        620        630        640        650 
GESEHKKEIQ FRLSAPNGSN IWDASNDYSY QGLTSNMQKT TKIPVFEDGV LVFGTLPDK 

« Hide

References

[1]"Molecular characterization of a beta-1,4-endoglucanase from an endophytic Bacillus pumilus strain."
Lima A.O.S., Quecine M.C., Fungaro M.H.P., Andreote F.D., Maccheroni W. Jr., Araujo W.L., Silva-Filho M.C., Pizzirani-Kleiner A.A., Azevedo J.L.
Appl. Microbiol. Biotechnol. 68:57-65(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: CL16.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY339624 Genomic DNA. Translation: AAQ91573.1.

3D structure databases

ProteinModelPortalQ5YLG1.
SMRQ5YLG1. Positions 45-657.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM3. Carbohydrate-Binding Module Family 3.
GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.50.10.10. 1 hit.
2.60.40.710. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamPF00942. CBM_3. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SMARTSM01067. CBM_3. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
SSF49384. SSF49384. 1 hit.
PROSITEPS51172. CBM3. 1 hit.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUNA_BACPU
AccessionPrimary (citable) accession number: Q5YLG1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 23, 2004
Last modified: October 16, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries