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Q5YLG1

- GUNA_BACPU

UniProt

Q5YLG1 - GUNA_BACPU

Protein

Endoglucanase A

Gene

eglA

Organism
Bacillus pumilus (Bacillus mesentericus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Active on carboxymethyl cellulose and carboxymethyl cellulose-RBB but not avicel, xanthan gum, carboxymethyl-curdulan-RBB or carboxymethyl-xylan-RBB.1 Publication

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.1 Publication

    Enzyme regulationi

    Strongly inhibited by ZnCl2 and by EDTA.1 Publication

    pH dependencei

    Optimum pH is 5-8.1 Publication

    Temperature dependencei

    Optimum temperature is 60 degrees Celsius. Retains more than 90% activity after 24 hours at 50 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei419 – 4191By similarity
    Active sitei457 – 4571By similarity
    Active sitei466 – 4661By similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC
    2. cellulose binding Source: InterPro

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Protein family/group databases

    CAZyiCBM3. Carbohydrate-Binding Module Family 3.
    GH9. Glycoside Hydrolase Family 9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase A (EC:3.2.1.4)
    Alternative name(s):
    Endo-1,4-beta-glucanase A
    Gene namesi
    Name:eglA
    OrganismiBacillus pumilus (Bacillus mesentericus)
    Taxonomic identifieri1408 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 659659Endoglucanase APRO_5000091857Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ5YLG1.
    SMRiQ5YLG1. Positions 45-657.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini501 – 658158CBM3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 500500CatalyticAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CBM3 (carbohydrate binding type-3) domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    2.60.40.710. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR008965. Carb-bd_dom.
    IPR001956. CBD_3.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view]
    PfamiPF00942. CBM_3. 1 hit.
    PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view]
    SMARTiSM01067. CBM_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    SSF49384. SSF49384. 1 hit.
    PROSITEiPS51172. CBM3. 1 hit.
    PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5YLG1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLIFETYLIL FKTVQITKRR IERRRLRLLN QCFTKKEGVS NREMASYNYV    50
    EVLQKSMLFY EAQRSGRLPE SNRLNWRGDS GLKDGKDVGH DLTGGWYDAG 100
    DHVKFGLPMA YSAAVLAWTV YEYREAYEEA ELLDEILDQI KWATDYFLKA 150
    HTGPNEFWAQ VGDGNADHAW WGPAEVMPMN RPAFKIDEHC PGTEVAAQTA 200
    AALAAGSIIF KETDASYAAK LLTHAKQLYA FADRYRGKYT DCVTNAQPFY 250
    NSWSGYVDEL IWGGIWLYLA TNEETYLNKA LKAVEEWPQD WDYTFTMSWD 300
    NTFFASQILL ARITKENRFI ESTERNLDYW TTGLVQNGKV ERITYTPGGL 350
    AWLDQWGSLR YAANAAFLAF VYADWVSDQE KKNRYQSFAI KQTHYMLGDN 400
    PLNRSYVVGF GQNSPKHPHH RTAHGSWSNQ LTNPPSHRHT LYGALVGGPN 450
    AQDQYDDDIS DYISNEVATD YNAAFTGNIA KMVQLFGEGQ SKLPNFPPKE 500
    QVEDEFFVEA AVMHNDTTST QVKAVLYNRS GWPARSSQTL SFRYYVNLSE 550
    VFAKGFTEKD IQVTAAYNEG ASLSPLKVYD ASSRVYFAEI DFTGVVISPR 600
    GESEHKKEIQ FRLSAPNGSN IWDASNDYSY QGLTSNMQKT TKIPVFEDGV 650
    LVFGTLPDK 659
    Length:659
    Mass (Da):74,985
    Last modified:November 23, 2004 - v1
    Checksum:i13DE360B374DA139
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY339624 Genomic DNA. Translation: AAQ91573.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY339624 Genomic DNA. Translation: AAQ91573.1 .

    3D structure databases

    ProteinModelPortali Q5YLG1.
    SMRi Q5YLG1. Positions 45-657.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM3. Carbohydrate-Binding Module Family 3.
    GH9. Glycoside Hydrolase Family 9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    2.60.40.710. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR008965. Carb-bd_dom.
    IPR001956. CBD_3.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view ]
    Pfami PF00942. CBM_3. 1 hit.
    PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view ]
    SMARTi SM01067. CBM_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    SSF49384. SSF49384. 1 hit.
    PROSITEi PS51172. CBM3. 1 hit.
    PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: CL16.

    Entry informationi

    Entry nameiGUNA_BACPU
    AccessioniPrimary (citable) accession number: Q5YLG1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3