Q5YLB5 (GYRA_NICBE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A, chloroplastic/mitochondrial
EC=5.99.1.3

Gene names

Name:

GYRA

Organism

Nicotiana benthamiana

Taxonomic identifier

4100 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	935 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Seems to play a critical role in chloroplast nucleoid partitioning by regulating DNA topology. DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner. Ref.1
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis By similarity.
Subcellular location	Plastid › chloroplast. Mitochondrion Ref.1.
Tissue specificity	Ubiquitous. Ref.1
Developmental stage	Up-regulated during seed germination and leaf expansion. Ref.1
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Chloroplast Mitochondrion Plastid
Domain	Transit peptide
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell chromosome Inferred from electronic annotation. Source: InterPro mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast and mitochondrion
	Chain	? – 935		DNA gyrase subunit A, chloroplastic/mitochondrial		PRO_0000247946
Sites
	Active site	204	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5YLB5 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 35CD6D27077119ED	FASTA	935	103,416
	10 20 30 40 50 60 MKLHTLNPQT PLTQSKPMAF STGITPSRFS GLRKTSSELR FLSSVTPPPR KQLRPVSARR 70 80 90 100 110 120 KEEEVGDEGN GSVILRDRGE NEDRNGGERV VLTELHKEAT EAYMSYAMSV LLGRALPDVR 130 140 150 160 170 180 DGLKPVHRRI LYAMHELGLS SKKPYKKCAR VVGEVLGKFH PHGDTAVYDS LVRMAQDFSL 190 200 210 220 230 240 RSPLIRGHGN FGSIDADPPA AMRYTECRLE ALTESMLLAD LEQNTVDFVP NFDNSQKEPS 250 260 270 280 290 300 LLPARVPNLL LNGASGIAVG MATNIPPHNL GELVDALSAL IHNPEATLQE LLEYMPGPDF 310 320 330 340 350 360 PTGGIIMGNI GILEAFRTGR GRVVIRGKTD IELLDSKTKR AAIIIQEIPY QTNKASLVEK 370 380 390 400 410 420 IADLVENKIL EGVSDIRDES DRSGMRIVIE LKRGSDPAIV LNNLYRLTAL QSSFSCNMVG 430 440 450 460 470 480 ILNGQPKLMG LKELLQAFLD FRCSVVERRA RFKLSQAQER NHIVEGIIVG LDNLDEVINT 490 500 510 520 530 540 IRKASSNALA AASLRKEFEL SEKQAEAILD ISLRRLTALE RNKFVEEGKS LRTQISKLEE 550 560 570 580 590 600 LLSSKKQILQ LIEEEAIEIK NKFFNPRRSM LEDTDSGDLE DIDVIPNEEM LLAISEKGYV 610 620 630 640 650 660 KRMKPDTFNL QNRGTIGKSV GKLRVNDAMS DFLVCRAHDK VLYFSDKGTV YSSPAYKIPE 670 680 690 700 710 720 CSRTAAGTPL VQILSLSDGE RITSIIPVSE FAADQYLVML TVNGYIKKVS LNYFASIRCT 730 740 750 760 770 780 GIIAIQLVPD DELKWVKCCS NNDFVAMASQ NGMVILTPCA NIRALGRNTR GSVAMRLKEG 790 800 810 820 830 840 DKVASMDIIP DALQKELDKT LEVQQRQYRS MKGPWLLFVS ESGYGKRVPV SRFRTSPLNR 850 860 870 880 890 900 VGLFGYKFSS EDCLAAVFVV GFSLGEDGES DEQVVLVSQS GTVNRIKVRD ISIQSRYARG 910 920 930 VILMRLEHAG KIQSASLISA ADADPEDEDA TAVAA

References

[1]

"DNA gyrase is involved in chloroplast nucleoid partitioning." Cho H.S., Lee S.S., Kim K.D., Hwang I., Lim J.-S., Park Y.-I., Pai H.-S. Plant Cell 16:2665-2682(2004) [PubMed: 15367714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY351386 mRNA. Translation: AAR07942.1.
3D structure databases
ProteinModelPortal	Q5YLB5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 2 hits.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_NICBE

Accession

Primary (citable) accession number: Q5YLB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program