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Q5YDB6 (CPL1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA polymerase II C-terminal domain phosphatase-like 1

Short name=FCP-like 1
EC=3.1.3.16
Alternative name(s):
Carboxyl-terminal phosphatase-like 1
Short name=AtCPL1
Short name=CTD phosphatase-like 1
Protein FIERY 2
Protein JASMONATE OVEREXPRESSING 1
Gene names
Name:CPL1
Synonyms:FRY2, JOE1
Ordered Locus Names:At4g21670
ORF Names:F17L22.130
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length967 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Processively dephosphorylates 'Ser-5' but not 'Ser-2' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II. Together with CPL2, required for male gametes fertility. Multifunctional regulator that modulates plant growth, stress, and phytohormones responses. Negative regulator of stress gene transcription involved in abscisic acid (ABA) mediated and jasmonic acid (JA) mediated signaling pathways, NaCl, osmotic stress, wounding, and cold resistance. Regulates negatively the expression of jasmonic acid (JA) biosynthetic genes in response to wounding. Ref.1 Ref.5 Ref.6 Ref.7 Ref.9 Ref.11

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate. Ref.6

Cofactor

Binds magnesium, cobalt or manganese ions. Ref.1

Subunit structure

Interacts with the FYVE domain-containing protein At1g20110, ANAC019, MYB3, MYB4 and MYB32. Ref.8

Subcellular location

Nucleus Ref.1 Ref.8.

Tissue specificity

Expressed at very low levels in roots, leaves, stems, flowers and siliques. Ref.7

Induction

Slightly repressed by ABA. Ref.7

Domain

DRBM domains are required for interactions with target transcription factors such as ANAC019 and MYB3. Ref.8

Disruption phenotype

Grows more rapidly and flower later than wild-type plants. Increased tolerance to salt stress and to ABA during seed germination but more sensitive to freezing damage at the seedling stage, by the enhanced expression of abiotic stress-induced genes. Hypersentitivity to MeJA, accumulates high levels of anthocyanin on medium containing MeJA. Confers wound hyperresponsiveness of JA-biosynthetic genes. Ref.1 Ref.5 Ref.6 Ref.7 Ref.11

Sequence similarities

Contains 2 DRBM (double-stranded RNA-binding) domains.

Contains 1 FCP1 homology domain.

Biophysicochemical properties

pH dependence:

Optimum pH is 5.5-6.0. Ref.1

Sequence caution

The sequence CAB36811.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAB81274.1 differs from that shown. Reason: Erroneous gene model prediction.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SEQ9ZVD07EBI-1786459,EBI-6553299

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 967967RNA polymerase II C-terminal domain phosphatase-like 1
PRO_0000376083

Regions

Domain151 – 401251FCP1 homology
Domain724 – 79269DRBM 1
Domain855 – 92571DRBM 2
Region945 – 96723Required for nuclear localization (NLS)

Experimental info

Mutagenesis1161E → K in cpl1-3; enhanced expression of abiotic stress-induced genes. Ref.11
Sequence conflict4461E → V in BAF01152. Ref.4
Sequence conflict6691E → V in BAD94401. Ref.4
Sequence conflict7971A → T in BAD94401. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q5YDB6 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 3FD10F0F3A73154D

FASTA967108,421
        10         20         30         40         50         60 
MYSNNRVEVF HGDGRLGELE IYPSRELNQQ QDDVMKQRKK KQREVMELAK MGIRISHFSQ 

        70         80         90        100        110        120 
SGERCPPLAI LTTISSCGLC FKLEASPSPA QESLSLFYSS CLRDNKTAVM LLGGEELHLV 

       130        140        150        160        170        180 
AMYSENIKND RPCFWAFSVA PGIYDSCLVM LNLRCLGIVF DLDETLVVAN TMRSFEDKID 

       190        200        210        220        230        240 
GFQRRINNEM DPQRLAVIVA EMKRYQDDKN LLKQYIESDQ VVENGEVIKV QSEIVPALSD 

       250        260        270        280        290        300 
NHQPLVRPLI RLQEKNIILT RINPMIRDTS VLVRMRPSWE ELRSYLTAKG RKRFEVYVCT 

       310        320        330        340        350        360 
MAERDYALEM WRLLDPEGNL INTNDLLARI VCVKSGFKKS LFNVFLDGTC HPKMALVIDD 

       370        380        390        400        410        420 
RLKVWDEKDQ PRVHVVPAFA PYYSPQAEAA ATPVLCVARN VACGVRGGFF RDFDDSLLPR 

       430        440        450        460        470        480 
IAEISYENDA EDIPSPPDVS HYLVSEDDTS GLNGNKDPLS FDGMADTEVE RRLKEAISAS 

       490        500        510        520        530        540 
SAVLPAANID PRIAAPVQFP MASASSVSVP VPVQVVQQAI QPSAMAFPSI PFQQPQQPTS 

       550        560        570        580        590        600 
IAKHLVPSEP SLQSSPAREE GEVPESELDP DTRRRLLILQ HGQDTRDPAP SEPSFPQRPP 

       610        620        630        640        650        660 
VQAPPSHVQS RNGWFPVEEE MDPAQIRRAV SKEYPLDSEM IHMEKHRPRH PSFFSKIDNS 

       670        680        690        700        710        720 
TQSDRMLHEN RRPPKESLRR DEQLRSNNNL PDSHPFYGED ASWNQSSSRN SDLDFLPERS 

       730        740        750        760        770        780 
VSATETSADV LHGIAIKCGA KVEYKPSLVS STDLRFSVEA WLSNQKIGEG IGKSRREALH 

       790        800        810        820        830        840 
KAAEASIQNL ADGYMRANGD PGPSHRDATP FTNENISMGN ANALNNQPFA RDETALPVSS 

       850        860        870        880        890        900 
RPTDPRLEGS MRHTGSITAL RELCASEGLE MAFQSQRQLP SDMVHRDELH AQVEIDGRVV 

       910        920        930        940        950        960 
GEGVGSTWDE ARMQAAERAL SSVRSMLGQP LHKRQGSPRS FGGMSNKRLK PDFQRSLQRM 


PSSGRYS 

« Hide

References

« Hide 'large scale' references
[1]"Arabidopsis C-terminal domain phosphatase-like 1 and 2 are essential Ser-5-specific C-terminal domain phosphatases."
Koiwa H., Hausmann S., Bang W.Y., Ueda A., Kondo N., Hiraguri A., Fukuhara T., Bahk J.D., Yun D.-J., Bressan R.A., Hasegawa P.M., Shuman S.
Proc. Natl. Acad. Sci. U.S.A. 101:14539-14544(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, COFACTOR.
[2]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Fusion genetic analysis of jasmonate-signalling mutants in Arabidopsis."
Jensen A.B., Raventos D., Mundy J.
Plant J. 29:595-606(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[6]"C-terminal domain phosphatase-like family members (AtCPLs) differentially regulate Arabidopsis thaliana abiotic stress signaling, growth, and development."
Koiwa H., Barb A.W., Xiong L., Li F., McCully M.G., Lee B.-H., Sokolchik I., Zhu J., Gong Z., Reddy M., Sharkhuu A., Manabe Y., Yokoi S., Zhu J.-K., Bressan R.A., Hasegawa P.M.
Proc. Natl. Acad. Sci. U.S.A. 99:10893-10898(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
[7]"Repression of stress-responsive genes by FIERY2, a novel transcriptional regulator in Arabidopsis."
Xiong L., Lee H., Ishitani M., Tanaka Y., Stevenson B., Koiwa H., Bressan R.A., Hasegawa P.M., Zhu J.-K.
Proc. Natl. Acad. Sci. U.S.A. 99:10899-10904(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.
[8]"The C-terminal region (640-967) of Arabidopsis CPL1 interacts with the abiotic stress- and ABA-responsive transcription factors."
Bang W.Y., Kim S.W., Jeong I.S., Koiwa H., Bahk J.D.
Biochem. Biophys. Res. Commun. 372:907-912(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH AT1G20110; ANAC019; MYB3; MYB4 AND MYB32, SUBCELLULAR LOCATION, DRBM DOMAINS.
[9]"The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses."
Ueda A., Li P., Feng Y., Vikram M., Kim S., Kang C.H., Kang J.S., Bahk J.D., Lee S.Y., Fukuhara T., Staswick P.E., Pepper A.E., Koiwa H.
Plant Mol. Biol. 67:683-697(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Evolutionary radiation pattern of novel protein phosphatases revealed by analysis of protein data from the completely sequenced genomes of humans, green algae, and higher plants."
Kerk D., Templeton G., Moorhead G.B.G.
Plant Physiol. 146:351-367(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[11]"CTD phosphatases in the attenuation of wound-induced transcription of jasmonic acid biosynthetic genes in Arabidopsis."
Matsuda O., Sakamoto H., Nakao Y., Oda K., Iba K.
Plant J. 57:96-108(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLU-116.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY557186 mRNA. Translation: AAT52022.1.
AL035527 Genomic DNA. Translation: CAB36811.1. Sequence problems.
AL161555 Genomic DNA. Translation: CAB81274.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84488.1.
AK221944 mRNA. Translation: BAD94401.1.
AK229289 mRNA. Translation: BAF01152.1.
PIRT05842.
RefSeqNP_193898.3. NM_118287.4.
UniGeneAt.2700.
At.32611.

3D structure databases

ProteinModelPortalQ5YDB6.
SMRQ5YDB6. Positions 260-380, 727-790, 853-925.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid13543. 11 interactions.
IntActQ5YDB6. 12 interactions.

Proteomic databases

PaxDbQ5YDB6.
PRIDEQ5YDB6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G21670.1; AT4G21670.1; AT4G21670.
GeneID828254.
KEGGath:AT4G21670.

Organism-specific databases

TAIRAT4G21670.

Phylogenomic databases

eggNOGNOG244866.
HOGENOMHOG000078695.
InParanoidQ5YDB6.
OMAPSERCPP.
PhylomeDBQ5YDB6.
ProtClustDBCLSN2681612.

Enzyme and pathway databases

BioCycARA:AT4G21670-MONOMER.

Gene expression databases

GenevestigatorQ5YDB6.

Family and domain databases

Gene3D3.30.160.20. 2 hits.
3.40.50.1000. 2 hits.
InterProIPR014720. dsRNA-bd_dom.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamPF00035. dsrm. 2 hits.
PF03031. NIF. 1 hit.
[Graphical view]
SMARTSM00577. CPDc. 1 hit.
SM00358. DSRM. 2 hits.
[Graphical view]
SUPFAMSSF56784. SSF56784. 2 hits.
PROSITEPS50137. DS_RBD. 2 hits.
PS50969. FCP1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCPL1_ARATH
AccessionPrimary (citable) accession number: Q5YDB6
Secondary accession number(s): Q0WNZ7, Q56WT8, Q9SVT0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names