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Q5YDB6

- CPL1_ARATH

UniProt

Q5YDB6 - CPL1_ARATH

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Protein

RNA polymerase II C-terminal domain phosphatase-like 1

Gene

CPL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Processively dephosphorylates 'Ser-5' but not 'Ser-2' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II. Together with CPL2, required for male gametes fertility. Multifunctional regulator that modulates plant growth, stress, and phytohormones responses. Negative regulator of stress gene transcription involved in abscisic acid (ABA) mediated and jasmonic acid (JA) mediated signaling pathways, NaCl, osmotic stress, wounding, and cold resistance. Regulates negatively the expression of jasmonic acid (JA) biosynthetic genes in response to wounding.6 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Cofactori

Mg2+1 Publication, Co2+1 Publication, Mn2+1 PublicationNote: Binds Mg(2+), Co(2+) or Mn(2+).1 Publication

pH dependencei

Optimum pH is 5.5-6.0.1 Publication

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphatase activity Source: TAIR
  3. phosphoprotein phosphatase activity Source: TAIR
  4. phosphoserine phosphatase activity Source: UniProtKB
  5. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. abscisic acid-activated signaling pathway Source: TAIR
  2. dephosphorylation Source: GOC
  3. multicellular organismal development Source: UniProtKB-KW
  4. negative regulation of transcription, DNA-templated Source: TAIR
  5. response to salt stress Source: TAIR
  6. response to wounding Source: UniProtKB
  7. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Repressor

Keywords - Biological processi

Abscisic acid signaling pathway, Jasmonic acid signaling pathway, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciARA:AT4G21670-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II C-terminal domain phosphatase-like 1 (EC:3.1.3.16)
Short name:
FCP-like 1
Alternative name(s):
Carboxyl-terminal phosphatase-like 1
Short name:
AtCPL1
Short name:
CTD phosphatase-like 1
Protein FIERY 2
Protein JASMONATE OVEREXPRESSING 1
Gene namesi
Name:CPL1
Synonyms:FRY2, JOE1
Ordered Locus Names:At4g21670
ORF Names:F17L22.130
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G21670.

Subcellular locationi

Nucleus 2 Publications

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Grows more rapidly and flower later than wild-type plants. Increased tolerance to salt stress and to ABA during seed germination but more sensitive to freezing damage at the seedling stage, by the enhanced expression of abiotic stress-induced genes. Hypersentitivity to MeJA, accumulates high levels of anthocyanin on medium containing MeJA. Confers wound hyperresponsiveness of JA-biosynthetic genes.5 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi116 – 1161E → K in cpl1-3; enhanced expression of abiotic stress-induced genes. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 967967RNA polymerase II C-terminal domain phosphatase-like 1PRO_0000376083Add
BLAST

Proteomic databases

PaxDbiQ5YDB6.
PRIDEiQ5YDB6.

Expressioni

Tissue specificityi

Expressed at very low levels in roots, leaves, stems, flowers and siliques.1 Publication

Inductioni

Slightly repressed by ABA.1 Publication

Gene expression databases

GenevestigatoriQ5YDB6.

Interactioni

Subunit structurei

Interacts with the FYVE domain-containing protein At1g20110, ANAC019, MYB3, MYB4 and MYB32. Binds to DMS3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SEQ9ZVD07EBI-1786459,EBI-6553299

Protein-protein interaction databases

BioGridi13543. 11 interactions.
IntActiQ5YDB6. 12 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ5YDB6.
SMRiQ5YDB6. Positions 854-925.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini151 – 401251FCP1 homologyPROSITE-ProRule annotationAdd
BLAST
Domaini724 – 79269DRBM 1PROSITE-ProRule annotationAdd
BLAST
Domaini855 – 92571DRBM 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni945 – 96723Required for nuclear localization (NLS)Add
BLAST

Domaini

DRBM domains are required for interactions with target transcription factors such as ANAC019 and MYB3.

Sequence similaritiesi

Contains 2 DRBM (double-stranded RNA-binding) domains.PROSITE-ProRule annotation
Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG244866.
HOGENOMiHOG000078695.
InParanoidiQ5YDB6.
OMAiSFDGMAD.
PhylomeDBiQ5YDB6.

Family and domain databases

Gene3Di3.30.160.20. 2 hits.
3.40.50.1000. 2 hits.
InterProiIPR014720. dsRNA-bd_dom.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamiPF00035. dsrm. 2 hits.
PF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
SM00358. DSRM. 2 hits.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
PROSITEiPS50137. DS_RBD. 2 hits.
PS50969. FCP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5YDB6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYSNNRVEVF HGDGRLGELE IYPSRELNQQ QDDVMKQRKK KQREVMELAK
60 70 80 90 100
MGIRISHFSQ SGERCPPLAI LTTISSCGLC FKLEASPSPA QESLSLFYSS
110 120 130 140 150
CLRDNKTAVM LLGGEELHLV AMYSENIKND RPCFWAFSVA PGIYDSCLVM
160 170 180 190 200
LNLRCLGIVF DLDETLVVAN TMRSFEDKID GFQRRINNEM DPQRLAVIVA
210 220 230 240 250
EMKRYQDDKN LLKQYIESDQ VVENGEVIKV QSEIVPALSD NHQPLVRPLI
260 270 280 290 300
RLQEKNIILT RINPMIRDTS VLVRMRPSWE ELRSYLTAKG RKRFEVYVCT
310 320 330 340 350
MAERDYALEM WRLLDPEGNL INTNDLLARI VCVKSGFKKS LFNVFLDGTC
360 370 380 390 400
HPKMALVIDD RLKVWDEKDQ PRVHVVPAFA PYYSPQAEAA ATPVLCVARN
410 420 430 440 450
VACGVRGGFF RDFDDSLLPR IAEISYENDA EDIPSPPDVS HYLVSEDDTS
460 470 480 490 500
GLNGNKDPLS FDGMADTEVE RRLKEAISAS SAVLPAANID PRIAAPVQFP
510 520 530 540 550
MASASSVSVP VPVQVVQQAI QPSAMAFPSI PFQQPQQPTS IAKHLVPSEP
560 570 580 590 600
SLQSSPAREE GEVPESELDP DTRRRLLILQ HGQDTRDPAP SEPSFPQRPP
610 620 630 640 650
VQAPPSHVQS RNGWFPVEEE MDPAQIRRAV SKEYPLDSEM IHMEKHRPRH
660 670 680 690 700
PSFFSKIDNS TQSDRMLHEN RRPPKESLRR DEQLRSNNNL PDSHPFYGED
710 720 730 740 750
ASWNQSSSRN SDLDFLPERS VSATETSADV LHGIAIKCGA KVEYKPSLVS
760 770 780 790 800
STDLRFSVEA WLSNQKIGEG IGKSRREALH KAAEASIQNL ADGYMRANGD
810 820 830 840 850
PGPSHRDATP FTNENISMGN ANALNNQPFA RDETALPVSS RPTDPRLEGS
860 870 880 890 900
MRHTGSITAL RELCASEGLE MAFQSQRQLP SDMVHRDELH AQVEIDGRVV
910 920 930 940 950
GEGVGSTWDE ARMQAAERAL SSVRSMLGQP LHKRQGSPRS FGGMSNKRLK
960
PDFQRSLQRM PSSGRYS
Length:967
Mass (Da):108,421
Last modified:November 23, 2004 - v1
Checksum:i3FD10F0F3A73154D
GO

Sequence cautioni

The sequence CAB36811.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB81274.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti446 – 4461E → V in BAF01152. 1 PublicationCurated
Sequence conflicti669 – 6691E → V in BAD94401. 1 PublicationCurated
Sequence conflicti797 – 7971A → T in BAD94401. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY557186 mRNA. Translation: AAT52022.1.
AL035527 Genomic DNA. Translation: CAB36811.1. Sequence problems.
AL161555 Genomic DNA. Translation: CAB81274.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84488.1.
AK221944 mRNA. Translation: BAD94401.1.
AK229289 mRNA. Translation: BAF01152.1.
PIRiT05842.
RefSeqiNP_193898.3. NM_118287.4.
UniGeneiAt.2700.
At.32611.

Genome annotation databases

EnsemblPlantsiAT4G21670.1; AT4G21670.1; AT4G21670.
GeneIDi828254.
KEGGiath:AT4G21670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY557186 mRNA. Translation: AAT52022.1 .
AL035527 Genomic DNA. Translation: CAB36811.1 . Sequence problems.
AL161555 Genomic DNA. Translation: CAB81274.1 . Sequence problems.
CP002687 Genomic DNA. Translation: AEE84488.1 .
AK221944 mRNA. Translation: BAD94401.1 .
AK229289 mRNA. Translation: BAF01152.1 .
PIRi T05842.
RefSeqi NP_193898.3. NM_118287.4.
UniGenei At.2700.
At.32611.

3D structure databases

ProteinModelPortali Q5YDB6.
SMRi Q5YDB6. Positions 854-925.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 13543. 11 interactions.
IntActi Q5YDB6. 12 interactions.

Proteomic databases

PaxDbi Q5YDB6.
PRIDEi Q5YDB6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G21670.1 ; AT4G21670.1 ; AT4G21670 .
GeneIDi 828254.
KEGGi ath:AT4G21670.

Organism-specific databases

TAIRi AT4G21670.

Phylogenomic databases

eggNOGi NOG244866.
HOGENOMi HOG000078695.
InParanoidi Q5YDB6.
OMAi SFDGMAD.
PhylomeDBi Q5YDB6.

Enzyme and pathway databases

BioCyci ARA:AT4G21670-MONOMER.

Gene expression databases

Genevestigatori Q5YDB6.

Family and domain databases

Gene3Di 3.30.160.20. 2 hits.
3.40.50.1000. 2 hits.
InterProi IPR014720. dsRNA-bd_dom.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view ]
Pfami PF00035. dsrm. 2 hits.
PF03031. NIF. 1 hit.
[Graphical view ]
SMARTi SM00577. CPDc. 1 hit.
SM00358. DSRM. 2 hits.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 2 hits.
PROSITEi PS50137. DS_RBD. 2 hits.
PS50969. FCP1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis C-terminal domain phosphatase-like 1 and 2 are essential Ser-5-specific C-terminal domain phosphatases."
    Koiwa H., Hausmann S., Bang W.Y., Ueda A., Kondo N., Hiraguri A., Fukuhara T., Bahk J.D., Yun D.-J., Bressan R.A., Hasegawa P.M., Shuman S.
    Proc. Natl. Acad. Sci. U.S.A. 101:14539-14544(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, COFACTOR.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Fusion genetic analysis of jasmonate-signalling mutants in Arabidopsis."
    Jensen A.B., Raventos D., Mundy J.
    Plant J. 29:595-606(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  6. "C-terminal domain phosphatase-like family members (AtCPLs) differentially regulate Arabidopsis thaliana abiotic stress signaling, growth, and development."
    Koiwa H., Barb A.W., Xiong L., Li F., McCully M.G., Lee B.-H., Sokolchik I., Zhu J., Gong Z., Reddy M., Sharkhuu A., Manabe Y., Yokoi S., Zhu J.-K., Bressan R.A., Hasegawa P.M.
    Proc. Natl. Acad. Sci. U.S.A. 99:10893-10898(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
  7. "Repression of stress-responsive genes by FIERY2, a novel transcriptional regulator in Arabidopsis."
    Xiong L., Lee H., Ishitani M., Tanaka Y., Stevenson B., Koiwa H., Bressan R.A., Hasegawa P.M., Zhu J.-K.
    Proc. Natl. Acad. Sci. U.S.A. 99:10899-10904(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.
  8. "The C-terminal region (640-967) of Arabidopsis CPL1 interacts with the abiotic stress- and ABA-responsive transcription factors."
    Bang W.Y., Kim S.W., Jeong I.S., Koiwa H., Bahk J.D.
    Biochem. Biophys. Res. Commun. 372:907-912(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AT1G20110; ANAC019; DMS3; MYB3; MYB4 AND MYB32, SUBCELLULAR LOCATION, DRBM DOMAINS.
  9. "The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses."
    Ueda A., Li P., Feng Y., Vikram M., Kim S., Kang C.H., Kang J.S., Bahk J.D., Lee S.Y., Fukuhara T., Staswick P.E., Pepper A.E., Koiwa H.
    Plant Mol. Biol. 67:683-697(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Evolutionary radiation pattern of novel protein phosphatases revealed by analysis of protein data from the completely sequenced genomes of humans, green algae, and higher plants."
    Kerk D., Templeton G., Moorhead G.B.G.
    Plant Physiol. 146:351-367(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  11. "CTD phosphatases in the attenuation of wound-induced transcription of jasmonic acid biosynthetic genes in Arabidopsis."
    Matsuda O., Sakamoto H., Nakao Y., Oda K., Iba K.
    Plant J. 57:96-108(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLU-116.

Entry informationi

Entry nameiCPL1_ARATH
AccessioniPrimary (citable) accession number: Q5YDB6
Secondary accession number(s): Q0WNZ7, Q56WT8, Q9SVT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: November 23, 2004
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3