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Q5YDB6

- CPL1_ARATH

UniProt

Q5YDB6 - CPL1_ARATH

Protein

RNA polymerase II C-terminal domain phosphatase-like 1

Gene

CPL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Processively dephosphorylates 'Ser-5' but not 'Ser-2' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II. Together with CPL2, required for male gametes fertility. Multifunctional regulator that modulates plant growth, stress, and phytohormones responses. Negative regulator of stress gene transcription involved in abscisic acid (ABA) mediated and jasmonic acid (JA) mediated signaling pathways, NaCl, osmotic stress, wounding, and cold resistance. Regulates negatively the expression of jasmonic acid (JA) biosynthetic genes in response to wounding.6 Publications

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

    Cofactori

    Binds magnesium, cobalt or manganese ions.1 Publication

    pH dependencei

    Optimum pH is 5.5-6.0.1 Publication

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phosphatase activity Source: TAIR
    3. phosphoprotein phosphatase activity Source: UniProtKB-EC
    4. phosphoserine phosphatase activity Source: UniProtKB
    5. protein binding Source: IntAct
    6. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. abscisic acid-activated signaling pathway Source: TAIR
    2. dephosphorylation Source: GOC
    3. multicellular organismal development Source: UniProtKB-KW
    4. negative regulation of transcription, DNA-templated Source: TAIR
    5. response to salt stress Source: TAIR
    6. response to wounding Source: UniProtKB
    7. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Hydrolase, Repressor

    Keywords - Biological processi

    Abscisic acid signaling pathway, Jasmonic acid signaling pathway, Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciARA:AT4G21670-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA polymerase II C-terminal domain phosphatase-like 1 (EC:3.1.3.16)
    Short name:
    FCP-like 1
    Alternative name(s):
    Carboxyl-terminal phosphatase-like 1
    Short name:
    AtCPL1
    Short name:
    CTD phosphatase-like 1
    Protein FIERY 2
    Protein JASMONATE OVEREXPRESSING 1
    Gene namesi
    Name:CPL1
    Synonyms:FRY2, JOE1
    Ordered Locus Names:At4g21670
    ORF Names:F17L22.130
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G21670.

    Subcellular locationi

    Nucleus 2 Publications

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Grows more rapidly and flower later than wild-type plants. Increased tolerance to salt stress and to ABA during seed germination but more sensitive to freezing damage at the seedling stage, by the enhanced expression of abiotic stress-induced genes. Hypersentitivity to MeJA, accumulates high levels of anthocyanin on medium containing MeJA. Confers wound hyperresponsiveness of JA-biosynthetic genes.5 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi116 – 1161E → K in cpl1-3; enhanced expression of abiotic stress-induced genes. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 967967RNA polymerase II C-terminal domain phosphatase-like 1PRO_0000376083Add
    BLAST

    Proteomic databases

    PaxDbiQ5YDB6.
    PRIDEiQ5YDB6.

    Expressioni

    Tissue specificityi

    Expressed at very low levels in roots, leaves, stems, flowers and siliques.1 Publication

    Inductioni

    Slightly repressed by ABA.1 Publication

    Gene expression databases

    GenevestigatoriQ5YDB6.

    Interactioni

    Subunit structurei

    Interacts with the FYVE domain-containing protein At1g20110, ANAC019, MYB3, MYB4 and MYB32. Binds to DMS3.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SEQ9ZVD07EBI-1786459,EBI-6553299

    Protein-protein interaction databases

    BioGridi13543. 11 interactions.
    IntActiQ5YDB6. 12 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5YDB6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini151 – 401251FCP1 homologyPROSITE-ProRule annotationAdd
    BLAST
    Domaini724 – 79269DRBM 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini855 – 92571DRBM 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni945 – 96723Required for nuclear localization (NLS)Add
    BLAST

    Domaini

    DRBM domains are required for interactions with target transcription factors such as ANAC019 and MYB3.

    Sequence similaritiesi

    Contains 2 DRBM (double-stranded RNA-binding) domains.PROSITE-ProRule annotation
    Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG244866.
    HOGENOMiHOG000078695.
    InParanoidiQ5YDB6.
    OMAiSFDGMAD.
    PhylomeDBiQ5YDB6.

    Family and domain databases

    Gene3Di3.30.160.20. 2 hits.
    3.40.50.1000. 2 hits.
    InterProiIPR014720. dsRNA-bd_dom.
    IPR023214. HAD-like_dom.
    IPR004274. NIF.
    [Graphical view]
    PfamiPF00035. dsrm. 2 hits.
    PF03031. NIF. 1 hit.
    [Graphical view]
    SMARTiSM00577. CPDc. 1 hit.
    SM00358. DSRM. 2 hits.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 2 hits.
    PROSITEiPS50137. DS_RBD. 2 hits.
    PS50969. FCP1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5YDB6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYSNNRVEVF HGDGRLGELE IYPSRELNQQ QDDVMKQRKK KQREVMELAK    50
    MGIRISHFSQ SGERCPPLAI LTTISSCGLC FKLEASPSPA QESLSLFYSS 100
    CLRDNKTAVM LLGGEELHLV AMYSENIKND RPCFWAFSVA PGIYDSCLVM 150
    LNLRCLGIVF DLDETLVVAN TMRSFEDKID GFQRRINNEM DPQRLAVIVA 200
    EMKRYQDDKN LLKQYIESDQ VVENGEVIKV QSEIVPALSD NHQPLVRPLI 250
    RLQEKNIILT RINPMIRDTS VLVRMRPSWE ELRSYLTAKG RKRFEVYVCT 300
    MAERDYALEM WRLLDPEGNL INTNDLLARI VCVKSGFKKS LFNVFLDGTC 350
    HPKMALVIDD RLKVWDEKDQ PRVHVVPAFA PYYSPQAEAA ATPVLCVARN 400
    VACGVRGGFF RDFDDSLLPR IAEISYENDA EDIPSPPDVS HYLVSEDDTS 450
    GLNGNKDPLS FDGMADTEVE RRLKEAISAS SAVLPAANID PRIAAPVQFP 500
    MASASSVSVP VPVQVVQQAI QPSAMAFPSI PFQQPQQPTS IAKHLVPSEP 550
    SLQSSPAREE GEVPESELDP DTRRRLLILQ HGQDTRDPAP SEPSFPQRPP 600
    VQAPPSHVQS RNGWFPVEEE MDPAQIRRAV SKEYPLDSEM IHMEKHRPRH 650
    PSFFSKIDNS TQSDRMLHEN RRPPKESLRR DEQLRSNNNL PDSHPFYGED 700
    ASWNQSSSRN SDLDFLPERS VSATETSADV LHGIAIKCGA KVEYKPSLVS 750
    STDLRFSVEA WLSNQKIGEG IGKSRREALH KAAEASIQNL ADGYMRANGD 800
    PGPSHRDATP FTNENISMGN ANALNNQPFA RDETALPVSS RPTDPRLEGS 850
    MRHTGSITAL RELCASEGLE MAFQSQRQLP SDMVHRDELH AQVEIDGRVV 900
    GEGVGSTWDE ARMQAAERAL SSVRSMLGQP LHKRQGSPRS FGGMSNKRLK 950
    PDFQRSLQRM PSSGRYS 967
    Length:967
    Mass (Da):108,421
    Last modified:November 23, 2004 - v1
    Checksum:i3FD10F0F3A73154D
    GO

    Sequence cautioni

    The sequence CAB36811.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAB81274.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti446 – 4461E → V in BAF01152. 1 PublicationCurated
    Sequence conflicti669 – 6691E → V in BAD94401. 1 PublicationCurated
    Sequence conflicti797 – 7971A → T in BAD94401. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY557186 mRNA. Translation: AAT52022.1.
    AL035527 Genomic DNA. Translation: CAB36811.1. Sequence problems.
    AL161555 Genomic DNA. Translation: CAB81274.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE84488.1.
    AK221944 mRNA. Translation: BAD94401.1.
    AK229289 mRNA. Translation: BAF01152.1.
    PIRiT05842.
    RefSeqiNP_193898.3. NM_118287.4.
    UniGeneiAt.2700.
    At.32611.

    Genome annotation databases

    EnsemblPlantsiAT4G21670.1; AT4G21670.1; AT4G21670.
    GeneIDi828254.
    KEGGiath:AT4G21670.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY557186 mRNA. Translation: AAT52022.1 .
    AL035527 Genomic DNA. Translation: CAB36811.1 . Sequence problems.
    AL161555 Genomic DNA. Translation: CAB81274.1 . Sequence problems.
    CP002687 Genomic DNA. Translation: AEE84488.1 .
    AK221944 mRNA. Translation: BAD94401.1 .
    AK229289 mRNA. Translation: BAF01152.1 .
    PIRi T05842.
    RefSeqi NP_193898.3. NM_118287.4.
    UniGenei At.2700.
    At.32611.

    3D structure databases

    ProteinModelPortali Q5YDB6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 13543. 11 interactions.
    IntActi Q5YDB6. 12 interactions.

    Proteomic databases

    PaxDbi Q5YDB6.
    PRIDEi Q5YDB6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G21670.1 ; AT4G21670.1 ; AT4G21670 .
    GeneIDi 828254.
    KEGGi ath:AT4G21670.

    Organism-specific databases

    TAIRi AT4G21670.

    Phylogenomic databases

    eggNOGi NOG244866.
    HOGENOMi HOG000078695.
    InParanoidi Q5YDB6.
    OMAi SFDGMAD.
    PhylomeDBi Q5YDB6.

    Enzyme and pathway databases

    BioCyci ARA:AT4G21670-MONOMER.

    Gene expression databases

    Genevestigatori Q5YDB6.

    Family and domain databases

    Gene3Di 3.30.160.20. 2 hits.
    3.40.50.1000. 2 hits.
    InterProi IPR014720. dsRNA-bd_dom.
    IPR023214. HAD-like_dom.
    IPR004274. NIF.
    [Graphical view ]
    Pfami PF00035. dsrm. 2 hits.
    PF03031. NIF. 1 hit.
    [Graphical view ]
    SMARTi SM00577. CPDc. 1 hit.
    SM00358. DSRM. 2 hits.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 2 hits.
    PROSITEi PS50137. DS_RBD. 2 hits.
    PS50969. FCP1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Arabidopsis C-terminal domain phosphatase-like 1 and 2 are essential Ser-5-specific C-terminal domain phosphatases."
      Koiwa H., Hausmann S., Bang W.Y., Ueda A., Kondo N., Hiraguri A., Fukuhara T., Bahk J.D., Yun D.-J., Bressan R.A., Hasegawa P.M., Shuman S.
      Proc. Natl. Acad. Sci. U.S.A. 101:14539-14544(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, COFACTOR.
    2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Fusion genetic analysis of jasmonate-signalling mutants in Arabidopsis."
      Jensen A.B., Raventos D., Mundy J.
      Plant J. 29:595-606(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    6. "C-terminal domain phosphatase-like family members (AtCPLs) differentially regulate Arabidopsis thaliana abiotic stress signaling, growth, and development."
      Koiwa H., Barb A.W., Xiong L., Li F., McCully M.G., Lee B.-H., Sokolchik I., Zhu J., Gong Z., Reddy M., Sharkhuu A., Manabe Y., Yokoi S., Zhu J.-K., Bressan R.A., Hasegawa P.M.
      Proc. Natl. Acad. Sci. U.S.A. 99:10893-10898(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
    7. "Repression of stress-responsive genes by FIERY2, a novel transcriptional regulator in Arabidopsis."
      Xiong L., Lee H., Ishitani M., Tanaka Y., Stevenson B., Koiwa H., Bressan R.A., Hasegawa P.M., Zhu J.-K.
      Proc. Natl. Acad. Sci. U.S.A. 99:10899-10904(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.
    8. "The C-terminal region (640-967) of Arabidopsis CPL1 interacts with the abiotic stress- and ABA-responsive transcription factors."
      Bang W.Y., Kim S.W., Jeong I.S., Koiwa H., Bahk J.D.
      Biochem. Biophys. Res. Commun. 372:907-912(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH AT1G20110; ANAC019; DMS3; MYB3; MYB4 AND MYB32, SUBCELLULAR LOCATION, DRBM DOMAINS.
    9. "The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses."
      Ueda A., Li P., Feng Y., Vikram M., Kim S., Kang C.H., Kang J.S., Bahk J.D., Lee S.Y., Fukuhara T., Staswick P.E., Pepper A.E., Koiwa H.
      Plant Mol. Biol. 67:683-697(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Evolutionary radiation pattern of novel protein phosphatases revealed by analysis of protein data from the completely sequenced genomes of humans, green algae, and higher plants."
      Kerk D., Templeton G., Moorhead G.B.G.
      Plant Physiol. 146:351-367(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.
    11. "CTD phosphatases in the attenuation of wound-induced transcription of jasmonic acid biosynthetic genes in Arabidopsis."
      Matsuda O., Sakamoto H., Nakao Y., Oda K., Iba K.
      Plant J. 57:96-108(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLU-116.

    Entry informationi

    Entry nameiCPL1_ARATH
    AccessioniPrimary (citable) accession number: Q5YDB6
    Secondary accession number(s): Q0WNZ7, Q56WT8, Q9SVT0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 26, 2009
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3