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Q5YDB5

- CPL2_ARATH

UniProt

Q5YDB5 - CPL2_ARATH

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Protein

RNA polymerase II C-terminal domain phosphatase-like 2

Gene

CPL2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Processively dephosphorylates 'Ser-5' but not 'Ser-2' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II. Together with CPL1, required for male gametes fertility. Multifunctional regulator that modulates plant growth, stress, and phytohormones responses. Positive transcription regulator of genes involved in high salinity resistance and auxin mediated signaling pathway.2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds magnesium, cobalt or manganese ions.1 Publication

pH dependencei

Optimum pH is 5.5-6.0.1 Publication

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-EC
  3. phosphoserine phosphatase activity Source: UniProtKB
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. auxin-activated signaling pathway Source: UniProtKB
  2. dephosphorylation Source: GOC
  3. multicellular organismal development Source: UniProtKB-KW
  4. positive regulation of transcription, DNA-templated Source: UniProtKB
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein, Hydrolase

Keywords - Biological processi

Auxin signaling pathway, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciARA:AT5G01270-MONOMER.
ARA:GQT-1723-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II C-terminal domain phosphatase-like 2 (EC:3.1.3.16)
Short name:
FCP-like 2
Alternative name(s):
Carboxyl-terminal phosphatase-like 2
Short name:
AtCPL2
Short name:
CTD phosphatase-like 2
Gene namesi
Name:CPL2
Ordered Locus Names:At5g01270
ORF Names:F7J8.250
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G01270.

Subcellular locationi

Nucleus 1 Publication. Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Leaf expansion defects, early flowering, low fertility, and increased salt (NaCl) sensitivity. Produces shorter hypocotyls than wild-type plants in the light, but not in the dark.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 770770RNA polymerase II C-terminal domain phosphatase-like 2PRO_0000376084Add
BLAST

Proteomic databases

PaxDbiQ5YDB5.
PRIDEiQ5YDB5.

Expressioni

Tissue specificityi

Expressed in seedlings, particularly in tissues undergoing cell division and expansion, petioles, cotyledons vasculature, leaves, shoot and root meristems, and flowers, especially in buds and organs vasculatures.1 Publication

Gene expression databases

GenevestigatoriQ5YDB5.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G01270.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ5YDB5.
SMRiQ5YDB5. Positions 654-722.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini134 – 385252FCP1 homologyPROSITE-ProRule annotationAdd
BLAST
Domaini656 – 72267DRBMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DRBM (double-stranded RNA-binding) domain.PROSITE-ProRule annotation
Contains 1 FCP1 homology domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG290259.
HOGENOMiHOG000078695.
InParanoidiQ5YDB5.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
3.40.50.1000. 3 hits.
InterProiIPR014720. dsRNA-bd_dom.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view]
PfamiPF00035. dsrm. 1 hit.
PF03031. NIF. 1 hit.
[Graphical view]
SMARTiSM00577. CPDc. 1 hit.
SM00358. DSRM. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
PROSITEiPS50137. DS_RBD. 1 hit.
PS50969. FCP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q5YDB5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNRLGHKSVV YHGDLRLGEL DVNHVSSSHE FRFPNDEIRI HHLSPAGERC
60 70 80 90 100
PPLAILQTIA SFAVRCKLES SAPVKSQELM HLHAVCFHEL KTAVVMLGDE
110 120 130 140 150
EIHLVAMPSK EKKFPCFWCF SVPSGLYDSC LRMLNTRCLS IVFDLDETLI
160 170 180 190 200
VANTMKSFED RIEALKSWIS REMDPVRING MSAELKRYMD DRMLLKQYID
210 220 230 240 250
NDYAFDNGVL LKAQPEEVRP TSDGQEKVCR PVIRLPEKNT VLTRIKPEIR
260 270 280 290 300
DTSVLVKLRP AWEELRSYLT AKTRKRFEVY VCTMAERDYA LEMWRLLDPE
310 320 330 340 350
AHLISLKELR DRIVCVKPDA KKSLLSVFNG GICHPKMAMV IDDRMKVWED
360 370 380 390 400
KDQPRVHVVS AYLPYYAPQA ETALVVPHLC VARNVACNVR GYFFKEFDES
410 420 430 440 450
LMSSISLVYY EDDVENLPPS PDVSNYVVIE DPGFASNGNI NAPPINEGMC
460 470 480 490 500
GGEVERRLNQ AAAADHSTLP ATSNAEQKPE TPKPQIAVIP NNASTATAAA
510 520 530 540 550
LLPSHKPSLL GAPRRDGFTF SDGGRPLMMR PGVDIRNQNF NQPPILAKIP
560 570 580 590 600
MQPPSSSMHS PGGWLVDDEN RPSFPGRPSG LYPSQFPHGT PGSAPVGPFA
610 620 630 640 650
HPSHLRSEEV AMDDDLKRQN PSRQTTEGGI SQNHLVSNGR EHHTDGGKSN
660 670 680 690 700
GGQSHLFVSA LQEIGRRCGS KVEFRTVIST NKELQFSVEV LFTGEKIGIG
710 720 730 740 750
MAKTKKDAHQ QAAENALRSL AEKYVAHVAP LARETEKGPE NDNGFLWESS
760 770
EDVSNKGLEE EAPKENISEL
Length:770
Mass (Da):85,953
Last modified:November 16, 2011 - v3
Checksum:i7672B4F18397CB14
GO

Sequence cautioni

The sequence CAB69855.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY557187 mRNA. Translation: AAT52023.1.
AL137189 Genomic DNA. Translation: CAB69855.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED90320.1.
PIRiT45967.
RefSeqiNP_195747.2. NM_120205.4. [Q5YDB5-1]
UniGeneiAt.21936.

Genome annotation databases

EnsemblPlantsiAT5G01270.1; AT5G01270.1; AT5G01270. [Q5YDB5-1]
AT5G01270.2; AT5G01270.2; AT5G01270.
GeneIDi831743.
KEGGiath:AT5G01270.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY557187 mRNA. Translation: AAT52023.1 .
AL137189 Genomic DNA. Translation: CAB69855.1 . Sequence problems.
CP002688 Genomic DNA. Translation: AED90320.1 .
PIRi T45967.
RefSeqi NP_195747.2. NM_120205.4. [Q5YDB5-1 ]
UniGenei At.21936.

3D structure databases

ProteinModelPortali Q5YDB5.
SMRi Q5YDB5. Positions 654-722.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT5G01270.1-P.

Proteomic databases

PaxDbi Q5YDB5.
PRIDEi Q5YDB5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G01270.1 ; AT5G01270.1 ; AT5G01270 . [Q5YDB5-1 ]
AT5G01270.2 ; AT5G01270.2 ; AT5G01270 .
GeneIDi 831743.
KEGGi ath:AT5G01270.

Organism-specific databases

TAIRi AT5G01270.

Phylogenomic databases

eggNOGi NOG290259.
HOGENOMi HOG000078695.
InParanoidi Q5YDB5.

Enzyme and pathway databases

BioCyci ARA:AT5G01270-MONOMER.
ARA:GQT-1723-MONOMER.

Gene expression databases

Genevestigatori Q5YDB5.

Family and domain databases

Gene3Di 3.30.160.20. 1 hit.
3.40.50.1000. 3 hits.
InterProi IPR014720. dsRNA-bd_dom.
IPR023214. HAD-like_dom.
IPR004274. NIF.
[Graphical view ]
Pfami PF00035. dsrm. 1 hit.
PF03031. NIF. 1 hit.
[Graphical view ]
SMARTi SM00577. CPDc. 1 hit.
SM00358. DSRM. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 2 hits.
PROSITEi PS50137. DS_RBD. 1 hit.
PS50969. FCP1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis C-terminal domain phosphatase-like 1 and 2 are essential Ser-5-specific C-terminal domain phosphatases."
    Koiwa H., Hausmann S., Bang W.Y., Ueda A., Kondo N., Hiraguri A., Fukuhara T., Bahk J.D., Yun D.-J., Bressan R.A., Hasegawa P.M., Shuman S.
    Proc. Natl. Acad. Sci. U.S.A. 101:14539-14544(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, COFACTOR.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses."
    Ueda A., Li P., Feng Y., Vikram M., Kim S., Kang C.H., Kang J.S., Bahk J.D., Lee S.Y., Fukuhara T., Staswick P.E., Pepper A.E., Koiwa H.
    Plant Mol. Biol. 67:683-697(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  5. "Evolutionary radiation pattern of novel protein phosphatases revealed by analysis of protein data from the completely sequenced genomes of humans, green algae, and higher plants."
    Kerk D., Templeton G., Moorhead G.B.G.
    Plant Physiol. 146:351-367(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.

Entry informationi

Entry nameiCPL2_ARATH
AccessioniPrimary (citable) accession number: Q5YDB5
Secondary accession number(s): F4K803, Q9LFA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: November 16, 2011
Last modified: October 29, 2014
This is version 73 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3