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Protein

Beta-1,3-N-acetylglucosaminyltransferase manic fringe

Gene

mfng

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules.By similarity

Catalytic activityi

Transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor.By similarity

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100SubstrateBy similarity1
Binding sitei172SubstrateBy similarity1
Metal bindingi173ManganeseBy similarity1
Active sitei261By similarity1
Metal bindingi285ManganeseBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Glycosyltransferase, Transferase
LigandManganese, Metal-binding

Protein family/group databases

CAZyiGT31 Glycosyltransferase Family 31

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-N-acetylglucosaminyltransferase manic fringe (EC:2.4.1.222)
Alternative name(s):
O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Gene namesi
Name:mfngImported
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 22

Organism-specific databases

ZFINiZDB-GENE-041130-1 mfng

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 5CytoplasmicSequence analysis5
Transmembranei6 – 26Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini27 – 360LumenalSequence analysisAdd BLAST334

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002504221 – 360Beta-1,3-N-acetylglucosaminyltransferase manic fringeAdd BLAST360

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi139 ↔ 150By similarity
Disulfide bondi168 ↔ 231By similarity
Glycosylationi214N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi335 ↔ 344By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ5YB40

Expressioni

Developmental stagei

First detected at the 12-somite stage. From the 20-somite stage, expressed in the otic vesicle.1 Publication

Gene expression databases

BgeeiENSDARG00000042925
ExpressionAtlasiQ5YB40 baseline

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000063007

Structurei

3D structure databases

ProteinModelPortaliQ5YB40
SMRiQ5YB40
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Sequence analysis

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II9W Eukaryota
ENOG410XS8Y LUCA
GeneTreeiENSGT00390000009913
HOGENOMiHOG000046678
HOVERGENiHBG007986
InParanoidiQ5YB40
KOiK05948
OMAiLRWFCHV
OrthoDBiEOG091G08XO
PhylomeDBiQ5YB40
TreeFamiTF324207

Family and domain databases

InterProiView protein in InterPro
IPR017374 Fringe
IPR003378 Fringe-like
PfamiView protein in Pfam
PF02434 Fringe, 1 hit
PIRSFiPIRSF038073 B-acetylgalactosaminyltfrase, 1 hit

Sequencei

Sequence statusi: Complete.

Q5YB40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILRRLFHVL PAFAFTLFIL VLLDLQLRTR SDQKPQNAHD GHQRTTFISE
60 70 80 90 100
TTAENQHRDG AHEKEKAEGQ KWTEVRSTPP LELSDIFIAV KTTGRFHKSR
110 120 130 140 150
LALLLETWIS ETKEHTYIFT DSPDADISSE GFNVVVTNCS PEHSHQALSC
160 170 180 190 200
KMAAEYDYFM ASYKKWLCHV DDDNYLNPGA LLSLLMAFPA DGDIYVGKPS
210 220 230 240 250
LDRPMRAQEL LEGNKTRDVH FWFATGGAGF CLSRNLAERM APWASGPRFE
260 270 280 290 300
QTSAVIMLPD DCTVGFIVER RLGISMIHSN MFHSHLENLL LLSPSDIPKQ
310 320 330 340 350
VTLSYGWFES KMNSVELKGV FTKDEDPSRF RTVHCLLYPT TSWCPVALKS
360
ALSWNQHVMH
Length:360
Mass (Da):40,964
Last modified:November 13, 2007 - v3
Checksum:i8A78F3524D7ED093
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40D → N in AAI15219 (Ref. 2) Curated1
Sequence conflicti125A → V in AAI15219 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY608926 mRNA Translation: AAT46070.1
BC115218 mRNA Translation: AAI15219.1
BC125890 mRNA Translation: AAI25891.1
RefSeqiNP_001007789.1, NM_001007788.1
UniGeneiDr.36704

Genome annotation databases

EnsembliENSDART00000063008; ENSDARP00000063007; ENSDARG00000042925
GeneIDi493633
KEGGidre:493633

Similar proteinsi

Entry informationi

Entry nameiMFNG_DANRE
AccessioniPrimary (citable) accession number: Q5YB40
Secondary accession number(s): A0JMI3, Q1RLZ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: November 13, 2007
Last modified: May 23, 2018
This is version 93 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health