Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-1,3-N-acetylglucosaminyltransferase manic fringe

Gene

mfng

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Glycosyltransferase involved in the elongation of O-linked ligands to activate Notch signaling. Possesses fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity (By similarity).By similarity

Catalytic activityi

Transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor.By similarity

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001SubstrateBy similarity
Binding sitei172 – 1721SubstrateBy similarity
Metal bindingi173 – 1731ManganeseBy similarity
Active sitei261 – 2611By similarity
Metal bindingi285 – 2851ManganeseBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-N-acetylglucosaminyltransferase manic fringe (EC:2.4.1.222)
Alternative name(s):
O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Gene namesi
Name:mfngImported
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 22

Organism-specific databases

ZFINiZDB-GENE-041130-1. mfng.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 55CytoplasmicSequence analysis
Transmembranei6 – 2621Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini27 – 360334LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360Beta-1,3-N-acetylglucosaminyltransferase manic fringePRO_0000250422Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi139 ↔ 150By similarity
Disulfide bondi168 ↔ 231By similarity
Glycosylationi214 – 2141N-linked (GlcNAc...)Sequence analysis
Disulfide bondi335 ↔ 344By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ5YB40.
PRIDEiQ5YB40.

Expressioni

Developmental stagei

First detected at the 12-somite stage. From the 20-somite stage, expressed in the otic vesicle.1 Publication

Gene expression databases

BgeeiQ5YB40.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000063007.

Structurei

3D structure databases

ProteinModelPortaliQ5YB40.
SMRiQ5YB40. Positions 81-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Sequence analysis

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II9W. Eukaryota.
ENOG410XS8Y. LUCA.
GeneTreeiENSGT00390000009913.
HOGENOMiHOG000046678.
HOVERGENiHBG007986.
InParanoidiQ5YB40.
KOiK05948.
OMAiKTTGRFH.
OrthoDBiEOG7ZSHTD.
PhylomeDBiQ5YB40.
TreeFamiTF324207.

Family and domain databases

InterProiIPR017374. Fringe.
IPR003378. Fringe-like.
[Graphical view]
PfamiPF02434. Fringe. 1 hit.
[Graphical view]
PIRSFiPIRSF038073. B-acetylgalactosaminyltfrase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5YB40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILRRLFHVL PAFAFTLFIL VLLDLQLRTR SDQKPQNAHD GHQRTTFISE
60 70 80 90 100
TTAENQHRDG AHEKEKAEGQ KWTEVRSTPP LELSDIFIAV KTTGRFHKSR
110 120 130 140 150
LALLLETWIS ETKEHTYIFT DSPDADISSE GFNVVVTNCS PEHSHQALSC
160 170 180 190 200
KMAAEYDYFM ASYKKWLCHV DDDNYLNPGA LLSLLMAFPA DGDIYVGKPS
210 220 230 240 250
LDRPMRAQEL LEGNKTRDVH FWFATGGAGF CLSRNLAERM APWASGPRFE
260 270 280 290 300
QTSAVIMLPD DCTVGFIVER RLGISMIHSN MFHSHLENLL LLSPSDIPKQ
310 320 330 340 350
VTLSYGWFES KMNSVELKGV FTKDEDPSRF RTVHCLLYPT TSWCPVALKS
360
ALSWNQHVMH
Length:360
Mass (Da):40,964
Last modified:November 13, 2007 - v3
Checksum:i8A78F3524D7ED093
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401D → N in AAI15219 (Ref. 2) Curated
Sequence conflicti125 – 1251A → V in AAI15219 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY608926 mRNA. Translation: AAT46070.1.
BC115218 mRNA. Translation: AAI15219.1.
BC125890 mRNA. Translation: AAI25891.1.
RefSeqiNP_001007789.1. NM_001007788.1.
UniGeneiDr.36704.

Genome annotation databases

EnsembliENSDART00000063008; ENSDARP00000063007; ENSDARG00000042925.
GeneIDi493633.
KEGGidre:493633.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY608926 mRNA. Translation: AAT46070.1.
BC115218 mRNA. Translation: AAI15219.1.
BC125890 mRNA. Translation: AAI25891.1.
RefSeqiNP_001007789.1. NM_001007788.1.
UniGeneiDr.36704.

3D structure databases

ProteinModelPortaliQ5YB40.
SMRiQ5YB40. Positions 81-346.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000063007.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Proteomic databases

PaxDbiQ5YB40.
PRIDEiQ5YB40.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000063008; ENSDARP00000063007; ENSDARG00000042925.
GeneIDi493633.
KEGGidre:493633.

Organism-specific databases

CTDi4242.
ZFINiZDB-GENE-041130-1. mfng.

Phylogenomic databases

eggNOGiENOG410II9W. Eukaryota.
ENOG410XS8Y. LUCA.
GeneTreeiENSGT00390000009913.
HOGENOMiHOG000046678.
HOVERGENiHBG007986.
InParanoidiQ5YB40.
KOiK05948.
OMAiKTTGRFH.
OrthoDBiEOG7ZSHTD.
PhylomeDBiQ5YB40.
TreeFamiTF324207.

Miscellaneous databases

PROiQ5YB40.

Gene expression databases

BgeeiQ5YB40.

Family and domain databases

InterProiIPR017374. Fringe.
IPR003378. Fringe-like.
[Graphical view]
PfamiPF02434. Fringe. 1 hit.
[Graphical view]
PIRSFiPIRSF038073. B-acetylgalactosaminyltfrase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and embryonic expression of three zebrafish fringe genes: lunatic fringe, radical fringe, and manic fringe."
    Qiu X., Xu H., Haddon C., Lewis J., Jiang Y.-J.
    Dev. Dyn. 231:621-630(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiMFNG_DANRE
AccessioniPrimary (citable) accession number: Q5YB40
Secondary accession number(s): A0JMI3, Q1RLZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: November 13, 2007
Last modified: June 8, 2016
This is version 81 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.