Q5XZD9 (FTHS_EUBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Synonyms:	thf

Organism

Eubacterium acidaminophilum

Taxonomic identifier

1731 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Eubacteriaceae › Eubacterium

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_0000199348
Regions
	Nucleotide binding	65 – 72	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5XZD9 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: C76CAAAA272D0986	FASTA	556	59,161
	10 20 30 40 50 60 MKTDVQIAQE AKMLPIMEVA KQIGLGEDDI ELYGKYKAKI SLDVYKRLAD KPDGKLVLVT 70 80 90 100 110 120 AINPTPAGEG KTTTNVGLSM GLNKIGKKTI TALNEPSLGP CFGVKGGAAG GGYAQVVPMD 130 140 150 160 170 180 DINLHFTGDI HAITTAHNLL AALMDNHIKQ GNALGIDINK ITWKRVLDMN DRALRDIVIG 190 200 210 220 230 240 LGGTANGIPR QDGFDITVAS EIMAIMCLAT SLSDLKDRLS RMIVGYTSRR LAVTADSLTL 250 260 270 280 290 300 RGALALLLKD ALKPNLVQTL ENTPAIIHGG PFANIAHGCN SVTTTKTALK IADYVVTEAG 310 320 330 340 350 360 FGADLGAEKF FDIKCRFADL KPDVAVIVAT VRALKNHGGV AKANLGAENM KALEDGFGNL 370 380 390 400 410 420 ERHIENVHKF GVPAVVAINA FPTDTEKELK FVEDACRKLG ADVVLSEVWA KGGEGGVELA 430 440 450 460 470 480 KKVVEVTEKG AAKFKPLYPA EMPLKQKIET IAKEIYRADG VEFSAKASKE LDKFEKLGFG 490 500 510 520 530 540 NLPICVAKTQ YSFSDNPNLK GAPKGFTVSV SNARISAGAG FIVVLTGDIM TMPGLPKVPA 550 ANHMDVLESG EIVGLF

References

[1]	"Genes involved in glycine decarboxylation reaction of Eubacterium acidaminophilum." Poehlein A., Lechel A., Groebe D., Andreesen J.R. Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY722711 Genomic DNA. Translation: AAU84895.1.
3D structure databases
ProteinModelPortal	Q5XZD9.
SMR	Q5XZD9. Positions 4-554.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_EUBAC

Accession

Primary (citable) accession number: Q5XZD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	November 23, 2004
Last modified:	April 3, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program