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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Eubacterium acidaminophilum
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathway:itetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi65 – 728ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Synonyms:thf
OrganismiEubacterium acidaminophilum
Taxonomic identifieri1731 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesEubacteriaceaeEubacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Formate--tetrahydrofolate ligasePRO_0000199348Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ5XZD9.
SMRiQ5XZD9. Positions 4-554.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5XZD9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTDVQIAQE AKMLPIMEVA KQIGLGEDDI ELYGKYKAKI SLDVYKRLAD
60 70 80 90 100
KPDGKLVLVT AINPTPAGEG KTTTNVGLSM GLNKIGKKTI TALNEPSLGP
110 120 130 140 150
CFGVKGGAAG GGYAQVVPMD DINLHFTGDI HAITTAHNLL AALMDNHIKQ
160 170 180 190 200
GNALGIDINK ITWKRVLDMN DRALRDIVIG LGGTANGIPR QDGFDITVAS
210 220 230 240 250
EIMAIMCLAT SLSDLKDRLS RMIVGYTSRR LAVTADSLTL RGALALLLKD
260 270 280 290 300
ALKPNLVQTL ENTPAIIHGG PFANIAHGCN SVTTTKTALK IADYVVTEAG
310 320 330 340 350
FGADLGAEKF FDIKCRFADL KPDVAVIVAT VRALKNHGGV AKANLGAENM
360 370 380 390 400
KALEDGFGNL ERHIENVHKF GVPAVVAINA FPTDTEKELK FVEDACRKLG
410 420 430 440 450
ADVVLSEVWA KGGEGGVELA KKVVEVTEKG AAKFKPLYPA EMPLKQKIET
460 470 480 490 500
IAKEIYRADG VEFSAKASKE LDKFEKLGFG NLPICVAKTQ YSFSDNPNLK
510 520 530 540 550
GAPKGFTVSV SNARISAGAG FIVVLTGDIM TMPGLPKVPA ANHMDVLESG

EIVGLF
Length:556
Mass (Da):59,161
Last modified:November 23, 2004 - v1
Checksum:iC76CAAAA272D0986
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY722711 Genomic DNA. Translation: AAU84895.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY722711 Genomic DNA. Translation: AAU84895.1.

3D structure databases

ProteinModelPortaliQ5XZD9.
SMRiQ5XZD9. Positions 4-554.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00193.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genes involved in glycine decarboxylation reaction of Eubacterium acidaminophilum."
    Poehlein A., Lechel A., Groebe D., Andreesen J.R.
    Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiFTHS_EUBAC
AccessioniPrimary (citable) accession number: Q5XZD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: November 23, 2004
Last modified: October 1, 2014
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.