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Q5XX07 (VP35_EBOSU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. This blockage is produced through the interaction with and inhibition host IKBKE and TBK1 producing a strong inhibition of the phosphorylation and activation of IRF3. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR By similarity.

Subunit structure

Homooligomer. Homomultimerizes via the coiled coil domain. Found in a trimeric complex in which VP35 bridges L and the nucleoprotein By similarity. May interact with VP40. Interacts with host IKBKE and TBK1; the interactions lead to inhibition of cellular antiviral response by blocking necessary interactions of IKBKE and TBK1 with their substrate IRF3 By similarity.

Subcellular location

Virion. Host cytoplasm By similarity.

Post-translational modification

Phosphorylated by host IKBKE By similarity.

Sequence similarities

Belongs to the filoviridae polymerase cofactor VP35 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329Polymerase cofactor VP35
PRO_0000245077

Regions

Coiled coil84 – 10825 Potential

Sequences

Sequence LengthMass (Da)Tools
Q5XX07 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 1B85B4EDF83FB011

FASTA32936,116
        10         20         30         40         50         60 
MQQDRTYRHH GPEVSGWFSE QLMTGKIPLT EVFVDVENKP SPAPITIISK NPKTTRKSDK 

        70         80         90        100        110        120 
QVQTDDASSL LTEEVKAAIN SVISAVRRQT NAIESLEGRV TTLEASLKPV QDMAKTISSL 

       130        140        150        160        170        180 
NRSCAEMVAK YDLLVMTTGR ATATAAATEA YWNEHGQAPP GPSLYEDDAI KAKLKDPNGK 

       190        200        210        220        230        240 
VPESVKQAYI NLDSTSALNE ENFGRPYISA KDLKEIIYDH LPGFGTAFHQ LVQVICKIGK 

       250        260        270        280        290        300 
DNNILDIIHA EFQASLAEGD SPQCALIQIT KRIPAFQDAS PPIVHIKSRG DIPKACQKSL 

       310        320 
RPVPPSPKID RGWVCIFQFQ DGKALGLKI 

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References

[1]"Complete genome sequence of an Ebola virus (Sudan species) responsible for a 2000 outbreak of human disease in Uganda."
Sanchez A., Rollin P.E.
Virus Res. 113:16-25(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY729654 Genomic RNA. Translation: AAU43884.1.
RefSeqYP_138521.1. NC_006432.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3160776.

Family and domain databases

InterProIPR002953. Filo_VP35.
[Graphical view]
PfamPF02097. Filo_VP35. 1 hit.
[Graphical view]
PIRSFPIRSF018326. VP35_FiloV. 1 hit.
PRINTSPR01240. FILOVP35.
ProtoNetSearch...

Entry information

Entry nameVP35_EBOSU
AccessionPrimary (citable) accession number: Q5XX07
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families