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Q5XX01

- L_EBOSU

UniProt

Q5XX01 - L_EBOSU

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Protein

RNA-directed RNA polymerase L

Gene

L

Organism
Sudan ebolavirus (strain Uganda-00) (SEBOV) (Sudan Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the glycoprotein (GP) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing, Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase L
Short name:
Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase
Including the following 3 domains:
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
mRNA guanylyltransferase (EC:2.7.7.-)
Gene namesi
Name:L
OrganismiSudan ebolavirus (strain Uganda-00) (SEBOV) (Sudan Ebola virus)
Taxonomic identifieri386033 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
ProteomesiUP000000277: Genome

Subcellular locationi

Host cytoplasm. Virion By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 22102210RNA-directed RNA polymerase LPRO_0000245049Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ5XX01.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini626 – 810185RdRp catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini1802 – 2000199Mononegavirus-type SAM-dependent 2'-O-MTasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 mononegavirus-type SAM-dependent 2'-O-MTase domain.PROSITE-ProRule annotation
Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR017235. RNA-dir_pol_L_filovirus.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view]
PfamiPF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view]
PIRSFiPIRSF037548. RNA_pol_Filoviridae. 1 hit.
TIGRFAMsiTIGR04198. paramyx_RNAcap. 1 hit.
PROSITEiPS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
PS51590. SAM_MT_MNV_L. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5XX01-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMATQHTQYP DARLSSPIVL DQCDLVTRAC GLYSEYSLNP KLKTCRLPKH
60 70 80 90 100
IYRLKYDTIV LRFISDVPVA TIPIDYIAPM LINVLADSKN VPLEPPCLSF
110 120 130 140 150
LDEIVNYTVQ DAAFLNYYMN QIKTQEGVIT DQLKQNIRRV IHKNRYLSAL
160 170 180 190 200
FFWHDLAILT RRGRMNRGNV RSTWFVTNEV VDILGYGDYI FWKIPIALLP
210 220 230 240 250
MNTANVPHAS TDWYQPNIFK EAIQGHTHII SVSTAEVLIM CKDLVTSRFN
260 270 280 290 300
TLLIAELARL EDPVSADYPL VDNIQSLYNA GDYLLSILGS EGYKIIKYLE
310 320 330 340 350
PLCLAKIQLC SQYTERKGRF LTQMHLAVIQ TLRELLLNRG LKKSQLSKIR
360 370 380 390 400
EFHQLLLRLR STPQQLCELF SIQKHWGHPV LHSEKAIQKV KNHATVLKAL
410 420 430 440 450
RPIIIFETYC VFKYSVAKHF FDSQGTWYSV ISDRCLTPGL NSYIRRNQFP
460 470 480 490 500
PLPMIKDLLW EFYHLDHPPL FSTKIISDLS IFIKDRATAV EQTCWDAVFE
510 520 530 540 550
PNVLGYSPPY RFNTKRVPEQ FLEQEDFSIE SVLQYAQELR YLLPQNRNFS
560 570 580 590 600
FSLKEKELNV GRTFGKLPYL TRNVQTLCEA LLADGLAKAF PSNMMVVTER
610 620 630 640 650
EQKESLLHQA SWHHTSDDFG EHATVRGSSF VTDLEKYNLA FRYEFTAPFI
660 670 680 690 700
KYCNQCYGVR NVFDWMHFLI PQCYMHVSDY YNPPHNVTLE NREYPPEGPS
710 720 730 740 750
AYRGHLGGIE GLQQKLWTSI SCAQISLVEI KTGFKLRSAV MGDNQCITVL
760 770 780 790 800
SVFPLESSPN EQERCAEDNA ARVAASLAKV TSACGIFLKP DETFVHSGFI
810 820 830 840 850
YFGKKQYLNG IQLPQSLKTA ARMAPLSDAI FDDLQGTLAS IGTAFERSIS
860 870 880 890 900
ETRHILPCRV AAAFHTYFSV RILQHHHLGF HKGSDLGQLA INKPLDFGTI
910 920 930 940 950
ALSLAVPQVL GGLSFLNPEK CLYRNLGDPV TSGLFQLKHY LSMVGMSDIF
960 970 980 990 1000
HALIAKSPGN CSAIDFVLNP GGLNVPGSQD LTSFLRQIVR RSITLSARNK
1010 1020 1030 1040 1050
LINTLFHASA DLEDELVCKW LLSSTPVMSR FAADIFSRTP SGKRLQILGY
1060 1070 1080 1090 1100
LEGTRTLLAS KMISNNAETP ILERLRKITL QRWNLWFSYL DHCDPALMEA
1110 1120 1130 1140 1150
IQPIKCTVDI AQILREYSWA HILDGRQLIG ATLPCIPEQF QTTWLKPYEQ
1160 1170 1180 1190 1200
CVECSSTNNS SPYVSVALKR NVVSAWPDAS RLGWTIGDGI PYIGSRTEDK
1210 1220 1230 1240 1250
IGQPAIKPRC PSAALREAIE LTSRLTWVTQ GSANSDQLIR PFLEARVNLS
1260 1270 1280 1290 1300
VQEILQMTPS HYSGNIVHRY NDQYSPHSFM ANRMSNTATR LMVSTNTLGE
1310 1320 1330 1340 1350
FSGGGQAARD SNIIFQNVIN FAVALYDIRF RNTCTSSIQY HRAHIHLTNC
1360 1370 1380 1390 1400
CTREVPAQYL TYTTTLNLDL SKYRNNELIY DSDPLRGGLN CNLSIDSPLM
1410 1420 1430 1440 1450
KGPRLNIIED DLIRLPHLSG WELAKTVLQS IISDSSNSST DPISSGETRS
1460 1470 1480 1490 1500
FTTHFLTYPK IGLLYSFGAL ISFYLGNTIL CTKKIGLTEF LYYLQNQIHN
1510 1520 1530 1540 1550
LSHRSLRIFK PTFRHSSVMS RLMDIDPNFS IYIGGTAGDR GLSDAARLFL
1560 1570 1580 1590 1600
RIAISTFLSF VEEWVIFRKA NIPLWVIYPL EGQRSDPPGE FLNRVKSLIV
1610 1620 1630 1640 1650
GTEDDKNKGS ILSRSGEKCS SNLVYNCKST ASNFFHASLA YWRGRHRPKK
1660 1670 1680 1690 1700
TIGATNATTA PHIILPLGNS DRPPGLDLNR NNDTFIPTRI KQIVQGDSRN
1710 1720 1730 1740 1750
DRTTTTRFPP KSRSTPTSAT EPPTKMYEGS TTHQGKLTDT HLDEDHNAKE
1760 1770 1780 1790 1800
FPSNPHRLVV PFFKLTKDGE YSIEPSPEES RSNIKGLLQH LRTMVDTTIY
1810 1820 1830 1840 1850
CRFTGIVSSM HYKLDEVLWE YNKFESAVTL AEGEGSGALL LIQKYGVKKL
1860 1870 1880 1890 1900
FLNTLATEHS IESEVISGYT TPRMLLPIMP KTHRGELEVI LNNSASQITD
1910 1920 1930 1940 1950
ITHRDWFSNQ KNRIPNDADI ITMDAETTEN LDRSRLYEAV YTIICNHINP
1960 1970 1980 1990 2000
KTLKVVILKV FLSDLDGMCW INNYLAPMFG SGYLIKPITS SAKSSEWYLC
2010 2020 2030 2040 2050
LSNLLSTLRT TQHQTQANCL HVVQCALQQQ VQRGSYWLSH LTKYTTSRLH
2060 2070 2080 2090 2100
NSYIAFGFPS LEKVLYHRYN LVDSRNGPLV SITRHLALLQ TEIRELVTDY
2110 2120 2130 2140 2150
NQLRQSRTQT YHFIKTSKGR ITKLVNDYLR FELVIRALKN NSTWHHELYL
2160 2170 2180 2190 2200
LPELIGVCHR FNHTRNCTCS ERFLVQTLYL HRMSDAEIKL MDRLTSLVNM
2210
FPEGFRSSSV
Length:2,210
Mass (Da):251,294
Last modified:November 23, 2004 - v1
Checksum:i9FCCAF21F7AA0078
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY729654 Genomic RNA. Translation: AAU43890.1.
RefSeqiYP_138527.1. NC_006432.1.

Genome annotation databases

GeneIDi3160771.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY729654 Genomic RNA. Translation: AAU43890.1 .
RefSeqi YP_138527.1. NC_006432.1.

3D structure databases

ProteinModelPortali Q5XX01.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3160771.

Family and domain databases

InterProi IPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR017235. RNA-dir_pol_L_filovirus.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view ]
Pfami PF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view ]
PIRSFi PIRSF037548. RNA_pol_Filoviridae. 1 hit.
TIGRFAMsi TIGR04198. paramyx_RNAcap. 1 hit.
PROSITEi PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
PS51590. SAM_MT_MNV_L. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of an Ebola virus (Sudan species) responsible for a 2000 outbreak of human disease in Uganda."
    Sanchez A., Rollin P.E.
    Virus Res. 113:16-25(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiL_EBOSU
AccessioniPrimary (citable) accession number: Q5XX01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: November 23, 2004
Last modified: October 29, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3