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Q5XX01

- L_EBOSU

UniProt

Q5XX01 - L_EBOSU

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Protein
RNA-directed RNA polymerase L
Gene
L
Organism
Sudan ebolavirus (strain Uganda-00) (SEBOV) (Sudan Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the glycoprotein (GP) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  3. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    mRNA capping, mRNA processing, Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase L
    Short name:
    Protein L
    Alternative name(s):
    Large structural protein
    Replicase
    Transcriptase
    Including the following 3 domains:
    mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
    mRNA guanylyltransferase (EC:2.7.7.-)
    Gene namesi
    Name:L
    OrganismiSudan ebolavirus (strain Uganda-00) (SEBOV) (Sudan Ebola virus)
    Taxonomic identifieri386033 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
    Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
    Homo sapiens (Human) [TaxID: 9606]
    Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
    ProteomesiUP000000277: Genome

    Subcellular locationi

    Host cytoplasm. Virion By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. virion Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Host cytoplasm, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 22102210RNA-directed RNA polymerase L
    PRO_0000245049Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ5XX01.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini626 – 810185RdRp catalytic
    Add
    BLAST
    Domaini1802 – 2000199Mononegavirus-type SAM-dependent 2'-O-MTase
    Add
    BLAST

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR026890. Mononeg_mRNAcap.
    IPR014023. Mononeg_RNA_pol_cat.
    IPR017235. RNA-dir_pol_L_filovirus.
    IPR025786. RNA-direct_RNA_pol_L.
    [Graphical view]
    PfamiPF14318. Mononeg_mRNAcap. 1 hit.
    PF00946. Mononeg_RNA_pol. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037548. RNA_pol_Filoviridae. 1 hit.
    TIGRFAMsiTIGR04198. paramyx_RNAcap. 1 hit.
    PROSITEiPS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
    PS51590. SAM_MT_MNV_L. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5XX01-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMATQHTQYP DARLSSPIVL DQCDLVTRAC GLYSEYSLNP KLKTCRLPKH     50
    IYRLKYDTIV LRFISDVPVA TIPIDYIAPM LINVLADSKN VPLEPPCLSF 100
    LDEIVNYTVQ DAAFLNYYMN QIKTQEGVIT DQLKQNIRRV IHKNRYLSAL 150
    FFWHDLAILT RRGRMNRGNV RSTWFVTNEV VDILGYGDYI FWKIPIALLP 200
    MNTANVPHAS TDWYQPNIFK EAIQGHTHII SVSTAEVLIM CKDLVTSRFN 250
    TLLIAELARL EDPVSADYPL VDNIQSLYNA GDYLLSILGS EGYKIIKYLE 300
    PLCLAKIQLC SQYTERKGRF LTQMHLAVIQ TLRELLLNRG LKKSQLSKIR 350
    EFHQLLLRLR STPQQLCELF SIQKHWGHPV LHSEKAIQKV KNHATVLKAL 400
    RPIIIFETYC VFKYSVAKHF FDSQGTWYSV ISDRCLTPGL NSYIRRNQFP 450
    PLPMIKDLLW EFYHLDHPPL FSTKIISDLS IFIKDRATAV EQTCWDAVFE 500
    PNVLGYSPPY RFNTKRVPEQ FLEQEDFSIE SVLQYAQELR YLLPQNRNFS 550
    FSLKEKELNV GRTFGKLPYL TRNVQTLCEA LLADGLAKAF PSNMMVVTER 600
    EQKESLLHQA SWHHTSDDFG EHATVRGSSF VTDLEKYNLA FRYEFTAPFI 650
    KYCNQCYGVR NVFDWMHFLI PQCYMHVSDY YNPPHNVTLE NREYPPEGPS 700
    AYRGHLGGIE GLQQKLWTSI SCAQISLVEI KTGFKLRSAV MGDNQCITVL 750
    SVFPLESSPN EQERCAEDNA ARVAASLAKV TSACGIFLKP DETFVHSGFI 800
    YFGKKQYLNG IQLPQSLKTA ARMAPLSDAI FDDLQGTLAS IGTAFERSIS 850
    ETRHILPCRV AAAFHTYFSV RILQHHHLGF HKGSDLGQLA INKPLDFGTI 900
    ALSLAVPQVL GGLSFLNPEK CLYRNLGDPV TSGLFQLKHY LSMVGMSDIF 950
    HALIAKSPGN CSAIDFVLNP GGLNVPGSQD LTSFLRQIVR RSITLSARNK 1000
    LINTLFHASA DLEDELVCKW LLSSTPVMSR FAADIFSRTP SGKRLQILGY 1050
    LEGTRTLLAS KMISNNAETP ILERLRKITL QRWNLWFSYL DHCDPALMEA 1100
    IQPIKCTVDI AQILREYSWA HILDGRQLIG ATLPCIPEQF QTTWLKPYEQ 1150
    CVECSSTNNS SPYVSVALKR NVVSAWPDAS RLGWTIGDGI PYIGSRTEDK 1200
    IGQPAIKPRC PSAALREAIE LTSRLTWVTQ GSANSDQLIR PFLEARVNLS 1250
    VQEILQMTPS HYSGNIVHRY NDQYSPHSFM ANRMSNTATR LMVSTNTLGE 1300
    FSGGGQAARD SNIIFQNVIN FAVALYDIRF RNTCTSSIQY HRAHIHLTNC 1350
    CTREVPAQYL TYTTTLNLDL SKYRNNELIY DSDPLRGGLN CNLSIDSPLM 1400
    KGPRLNIIED DLIRLPHLSG WELAKTVLQS IISDSSNSST DPISSGETRS 1450
    FTTHFLTYPK IGLLYSFGAL ISFYLGNTIL CTKKIGLTEF LYYLQNQIHN 1500
    LSHRSLRIFK PTFRHSSVMS RLMDIDPNFS IYIGGTAGDR GLSDAARLFL 1550
    RIAISTFLSF VEEWVIFRKA NIPLWVIYPL EGQRSDPPGE FLNRVKSLIV 1600
    GTEDDKNKGS ILSRSGEKCS SNLVYNCKST ASNFFHASLA YWRGRHRPKK 1650
    TIGATNATTA PHIILPLGNS DRPPGLDLNR NNDTFIPTRI KQIVQGDSRN 1700
    DRTTTTRFPP KSRSTPTSAT EPPTKMYEGS TTHQGKLTDT HLDEDHNAKE 1750
    FPSNPHRLVV PFFKLTKDGE YSIEPSPEES RSNIKGLLQH LRTMVDTTIY 1800
    CRFTGIVSSM HYKLDEVLWE YNKFESAVTL AEGEGSGALL LIQKYGVKKL 1850
    FLNTLATEHS IESEVISGYT TPRMLLPIMP KTHRGELEVI LNNSASQITD 1900
    ITHRDWFSNQ KNRIPNDADI ITMDAETTEN LDRSRLYEAV YTIICNHINP 1950
    KTLKVVILKV FLSDLDGMCW INNYLAPMFG SGYLIKPITS SAKSSEWYLC 2000
    LSNLLSTLRT TQHQTQANCL HVVQCALQQQ VQRGSYWLSH LTKYTTSRLH 2050
    NSYIAFGFPS LEKVLYHRYN LVDSRNGPLV SITRHLALLQ TEIRELVTDY 2100
    NQLRQSRTQT YHFIKTSKGR ITKLVNDYLR FELVIRALKN NSTWHHELYL 2150
    LPELIGVCHR FNHTRNCTCS ERFLVQTLYL HRMSDAEIKL MDRLTSLVNM 2200
    FPEGFRSSSV 2210
    Length:2,210
    Mass (Da):251,294
    Last modified:November 23, 2004 - v1
    Checksum:i9FCCAF21F7AA0078
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY729654 Genomic RNA. Translation: AAU43890.1.
    RefSeqiYP_138527.1. NC_006432.1.

    Genome annotation databases

    GeneIDi3160771.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY729654 Genomic RNA. Translation: AAU43890.1 .
    RefSeqi YP_138527.1. NC_006432.1.

    3D structure databases

    ProteinModelPortali Q5XX01.
    ModBasei Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3160771.

    Family and domain databases

    InterProi IPR026890. Mononeg_mRNAcap.
    IPR014023. Mononeg_RNA_pol_cat.
    IPR017235. RNA-dir_pol_L_filovirus.
    IPR025786. RNA-direct_RNA_pol_L.
    [Graphical view ]
    Pfami PF14318. Mononeg_mRNAcap. 1 hit.
    PF00946. Mononeg_RNA_pol. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037548. RNA_pol_Filoviridae. 1 hit.
    TIGRFAMsi TIGR04198. paramyx_RNAcap. 1 hit.
    PROSITEi PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
    PS51590. SAM_MT_MNV_L. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of an Ebola virus (Sudan species) responsible for a 2000 outbreak of human disease in Uganda."
      Sanchez A., Rollin P.E.
      Virus Res. 113:16-25(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiL_EBOSU
    AccessioniPrimary (citable) accession number: Q5XX01
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: November 23, 2004
    Last modified: April 16, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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