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Q5XX01

- L_EBOSU

UniProt

Q5XX01 - L_EBOSU

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Protein

RNA-directed RNA polymerase L

Gene
L
Organism
Sudan ebolavirus (strain Uganda-00) (SEBOV) (Sudan Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the glycoprotein (GP) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. mRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-EC
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing, Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase L
Short name:
Protein L
Alternative name(s):
Large structural protein
Replicase
Transcriptase
Including the following 3 domains:
mRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.56)
mRNA guanylyltransferase (EC:2.7.7.-)
Gene namesi
Name:L
OrganismiSudan ebolavirus (strain Uganda-00) (SEBOV) (Sudan Ebola virus)
Taxonomic identifieri386033 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
ProteomesiUP000000277: Genome

Subcellular locationi

Host cytoplasm. Virion By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. virion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 22102210RNA-directed RNA polymerase LPRO_0000245049Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ5XX01.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini626 – 810185RdRp catalyticAdd
BLAST
Domaini1802 – 2000199Mononegavirus-type SAM-dependent 2'-O-MTaseAdd
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR017235. RNA-dir_pol_L_filovirus.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view]
PfamiPF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view]
PIRSFiPIRSF037548. RNA_pol_Filoviridae. 1 hit.
TIGRFAMsiTIGR04198. paramyx_RNAcap. 1 hit.
PROSITEiPS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
PS51590. SAM_MT_MNV_L. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5XX01-1 [UniParc]FASTAAdd to Basket

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MMATQHTQYP DARLSSPIVL DQCDLVTRAC GLYSEYSLNP KLKTCRLPKH     50
IYRLKYDTIV LRFISDVPVA TIPIDYIAPM LINVLADSKN VPLEPPCLSF 100
LDEIVNYTVQ DAAFLNYYMN QIKTQEGVIT DQLKQNIRRV IHKNRYLSAL 150
FFWHDLAILT RRGRMNRGNV RSTWFVTNEV VDILGYGDYI FWKIPIALLP 200
MNTANVPHAS TDWYQPNIFK EAIQGHTHII SVSTAEVLIM CKDLVTSRFN 250
TLLIAELARL EDPVSADYPL VDNIQSLYNA GDYLLSILGS EGYKIIKYLE 300
PLCLAKIQLC SQYTERKGRF LTQMHLAVIQ TLRELLLNRG LKKSQLSKIR 350
EFHQLLLRLR STPQQLCELF SIQKHWGHPV LHSEKAIQKV KNHATVLKAL 400
RPIIIFETYC VFKYSVAKHF FDSQGTWYSV ISDRCLTPGL NSYIRRNQFP 450
PLPMIKDLLW EFYHLDHPPL FSTKIISDLS IFIKDRATAV EQTCWDAVFE 500
PNVLGYSPPY RFNTKRVPEQ FLEQEDFSIE SVLQYAQELR YLLPQNRNFS 550
FSLKEKELNV GRTFGKLPYL TRNVQTLCEA LLADGLAKAF PSNMMVVTER 600
EQKESLLHQA SWHHTSDDFG EHATVRGSSF VTDLEKYNLA FRYEFTAPFI 650
KYCNQCYGVR NVFDWMHFLI PQCYMHVSDY YNPPHNVTLE NREYPPEGPS 700
AYRGHLGGIE GLQQKLWTSI SCAQISLVEI KTGFKLRSAV MGDNQCITVL 750
SVFPLESSPN EQERCAEDNA ARVAASLAKV TSACGIFLKP DETFVHSGFI 800
YFGKKQYLNG IQLPQSLKTA ARMAPLSDAI FDDLQGTLAS IGTAFERSIS 850
ETRHILPCRV AAAFHTYFSV RILQHHHLGF HKGSDLGQLA INKPLDFGTI 900
ALSLAVPQVL GGLSFLNPEK CLYRNLGDPV TSGLFQLKHY LSMVGMSDIF 950
HALIAKSPGN CSAIDFVLNP GGLNVPGSQD LTSFLRQIVR RSITLSARNK 1000
LINTLFHASA DLEDELVCKW LLSSTPVMSR FAADIFSRTP SGKRLQILGY 1050
LEGTRTLLAS KMISNNAETP ILERLRKITL QRWNLWFSYL DHCDPALMEA 1100
IQPIKCTVDI AQILREYSWA HILDGRQLIG ATLPCIPEQF QTTWLKPYEQ 1150
CVECSSTNNS SPYVSVALKR NVVSAWPDAS RLGWTIGDGI PYIGSRTEDK 1200
IGQPAIKPRC PSAALREAIE LTSRLTWVTQ GSANSDQLIR PFLEARVNLS 1250
VQEILQMTPS HYSGNIVHRY NDQYSPHSFM ANRMSNTATR LMVSTNTLGE 1300
FSGGGQAARD SNIIFQNVIN FAVALYDIRF RNTCTSSIQY HRAHIHLTNC 1350
CTREVPAQYL TYTTTLNLDL SKYRNNELIY DSDPLRGGLN CNLSIDSPLM 1400
KGPRLNIIED DLIRLPHLSG WELAKTVLQS IISDSSNSST DPISSGETRS 1450
FTTHFLTYPK IGLLYSFGAL ISFYLGNTIL CTKKIGLTEF LYYLQNQIHN 1500
LSHRSLRIFK PTFRHSSVMS RLMDIDPNFS IYIGGTAGDR GLSDAARLFL 1550
RIAISTFLSF VEEWVIFRKA NIPLWVIYPL EGQRSDPPGE FLNRVKSLIV 1600
GTEDDKNKGS ILSRSGEKCS SNLVYNCKST ASNFFHASLA YWRGRHRPKK 1650
TIGATNATTA PHIILPLGNS DRPPGLDLNR NNDTFIPTRI KQIVQGDSRN 1700
DRTTTTRFPP KSRSTPTSAT EPPTKMYEGS TTHQGKLTDT HLDEDHNAKE 1750
FPSNPHRLVV PFFKLTKDGE YSIEPSPEES RSNIKGLLQH LRTMVDTTIY 1800
CRFTGIVSSM HYKLDEVLWE YNKFESAVTL AEGEGSGALL LIQKYGVKKL 1850
FLNTLATEHS IESEVISGYT TPRMLLPIMP KTHRGELEVI LNNSASQITD 1900
ITHRDWFSNQ KNRIPNDADI ITMDAETTEN LDRSRLYEAV YTIICNHINP 1950
KTLKVVILKV FLSDLDGMCW INNYLAPMFG SGYLIKPITS SAKSSEWYLC 2000
LSNLLSTLRT TQHQTQANCL HVVQCALQQQ VQRGSYWLSH LTKYTTSRLH 2050
NSYIAFGFPS LEKVLYHRYN LVDSRNGPLV SITRHLALLQ TEIRELVTDY 2100
NQLRQSRTQT YHFIKTSKGR ITKLVNDYLR FELVIRALKN NSTWHHELYL 2150
LPELIGVCHR FNHTRNCTCS ERFLVQTLYL HRMSDAEIKL MDRLTSLVNM 2200
FPEGFRSSSV 2210
Length:2,210
Mass (Da):251,294
Last modified:November 23, 2004 - v1
Checksum:i9FCCAF21F7AA0078
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY729654 Genomic RNA. Translation: AAU43890.1.
RefSeqiYP_138527.1. NC_006432.1.

Genome annotation databases

GeneIDi3160771.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY729654 Genomic RNA. Translation: AAU43890.1 .
RefSeqi YP_138527.1. NC_006432.1.

3D structure databases

ProteinModelPortali Q5XX01.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3160771.

Family and domain databases

InterProi IPR026890. Mononeg_mRNAcap.
IPR014023. Mononeg_RNA_pol_cat.
IPR017235. RNA-dir_pol_L_filovirus.
IPR025786. RNA-direct_RNA_pol_L.
[Graphical view ]
Pfami PF14318. Mononeg_mRNAcap. 1 hit.
PF00946. Mononeg_RNA_pol. 1 hit.
[Graphical view ]
PIRSFi PIRSF037548. RNA_pol_Filoviridae. 1 hit.
TIGRFAMsi TIGR04198. paramyx_RNAcap. 1 hit.
PROSITEi PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit.
PS51590. SAM_MT_MNV_L. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of an Ebola virus (Sudan species) responsible for a 2000 outbreak of human disease in Uganda."
    Sanchez A., Rollin P.E.
    Virus Res. 113:16-25(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiL_EBOSU
AccessioniPrimary (citable) accession number: Q5XX01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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