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Q5XUX0

- FBX31_HUMAN

UniProt

Q5XUX0 - FBX31_HUMAN

Protein

F-box only protein 31

Gene

FBXO31

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (01 May 2007)
      Previous versions | rss
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    Functioni

    Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. Specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. May act as a tumor suppressor.2 Publications

    Pathwayi

    GO - Molecular functioni

    1. cyclin binding Source: UniProtKB

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: UniProtKB
    2. cyclin catabolic process Source: UniProtKB
    3. mitotic G1 DNA damage checkpoint Source: UniProtKB
    4. protein ubiquitination Source: UniProtKB-UniPathway
    5. SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB

    Keywords - Biological processi

    Cell cycle, DNA damage, Ubl conjugation pathway

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    F-box only protein 31
    Gene namesi
    Name:FBXO31
    Synonyms:FBX14, FBX31
    ORF Names:PP2386
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:16510. FBXO31.

    Subcellular locationi

    GO - Cellular componenti

    1. SCF ubiquitin ligase complex Source: UniProtKB

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi278 – 2781S → A: Fails to accumulate following gamma-irradiation. 1 Publication
    Mutagenesisi400 – 4001S → A: No effect following gamma-irradiation. 1 Publication

    Organism-specific databases

    PharmGKBiPA28042.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 539539F-box only protein 31PRO_0000119921Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei33 – 331PhosphoserineBy similarity
    Modified residuei37 – 371PhosphothreonineBy similarity
    Modified residuei278 – 2781Phosphoserine; by ATM1 Publication

    Post-translational modificationi

    Phosphorylation at Ser-278 by ATM following gamma-irradiation results in its stabilization.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ5XUX0.
    PaxDbiQ5XUX0.
    PRIDEiQ5XUX0.

    PTM databases

    PhosphoSiteiQ5XUX0.

    Expressioni

    Tissue specificityi

    Highly expressed in brain. Expressed at moderate levels in most tissues, except bone marrow.1 Publication

    Developmental stagei

    Expression is cell-cycle regulated, and peaks at late G2 to early G1 phase (at protein level).1 Publication

    Inductioni

    By DNA damage. Increases after UV irradiation, X-ray irradiation, oxidative stress (H2O2) or addition of the chemotherapeutic DNA-damaging agents etoposide, adriamycin, cisplatin or fluorouracil.1 Publication

    Gene expression databases

    ArrayExpressiQ5XUX0.
    BgeeiQ5XUX0.
    CleanExiHS_FBXO31.
    GenevestigatoriQ5XUX0.

    Organism-specific databases

    HPAiHPA030150.

    Interactioni

    Subunit structurei

    Part of a SCF (SKP1-cullin-F-box) protein ligase complex.2 Publications

    Protein-protein interaction databases

    BioGridi122890. 8 interactions.
    DIPiDIP-60449N.
    IntActiQ5XUX0. 1 interaction.
    STRINGi9606.ENSP00000310841.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5XUX0.
    SMRiQ5XUX0. Positions 66-137.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini64 – 11047F-boxPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi58 – 636Poly-Pro

    Sequence similaritiesi

    Belongs to the FBXO31 family.Curated
    Contains 1 F-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG43393.
    HOGENOMiHOG000112543.
    HOVERGENiHBG071549.
    InParanoidiQ5XUX0.
    KOiK10308.
    OMAiPERTPGV.
    OrthoDBiEOG7CG6ZR.
    PhylomeDBiQ5XUX0.
    TreeFamiTF331818.

    Family and domain databases

    InterProiIPR001810. F-box_dom.
    IPR026941. FBXO31.
    [Graphical view]
    PANTHERiPTHR10706:SF126. PTHR10706:SF126. 1 hit.
    PfamiPF12937. F-box-like. 1 hit.
    [Graphical view]
    SMARTiSM00256. FBOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF81383. SSF81383. 1 hit.
    PROSITEiPS50181. FBOX. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q5XUX0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAVCARLCGV GPSRGCRRRQ QRRGPAETAA ADSEPDTDPE EERIEASAGV    50
    GGGLCAGPSP PPPRCSLLEL PPELLVEIFA SLPGTDLPSL AQVCTKFRRI 100
    LHTDTIWRRR CREEYGVCEN LRKLEITGVS CRDVYAKLLH RYRHILGLWQ 150
    PDIGPYGGLL NVVVDGLFII GWMYLPPHDP HVDDPMRFKP LFRIHLMERK 200
    AATVECMYGH KGPHHGHIQI VKKDEFSTKC NQTDHHRMSG GRQEEFRTWL 250
    REEWGRTLED IFHEHMQELI LMKFIYTSQY DNCLTYRRIY LPPSRPDDLI 300
    KPGLFKGTYG SHGLEIVMLS FHGRRARGTK ITGDPNIPAG QQTVEIDLRH 350
    RIQLPDLENQ RNFNELSRIV LEVRERVRQE QQEGGHEAGE GRGRQGPRES 400
    QPSPAQPRAE APSKGPDGTP GEDGGEPGDA VAAAEQPAQC GQGQPFVLPV 450
    GVSSRNEDYP RTCRMCFYGT GLIAGHGFTS PERTPGVFIL FDEDRFGFVW 500
    LELKSFSLYS RVQATFRNAD APSPQAFDEM LKNIQSLTS 539
    Length:539
    Mass (Da):60,664
    Last modified:May 1, 2007 - v2
    Checksum:iE833D63A361E7381
    GO
    Isoform 2 (identifier: Q5XUX0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-113: MAVCARLCGV...DTIWRRRCRE → MFLVT

    Note: No experimental confirmation available.

    Show »
    Length:431
    Mass (Da):49,039
    Checksum:i69E7352F7253F32C
    GO

    Sequence cautioni

    The sequence AAL55855.1 differs from that shown. Reason: Frameshift at position 496.
    The sequence AAH12748.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAB55929.2 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti138 – 1381L → V in AAU50679. (PubMed:15520277)Curated
    Sequence conflicti281 – 2811D → E in AAU50679. (PubMed:15520277)Curated
    Sequence conflicti466 – 4661C → S in AAU50679. (PubMed:15520277)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 113113MAVCA…RRCRE → MFLVT in isoform 2. 1 PublicationVSP_037469Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY736035 mRNA. Translation: AAU50679.1.
    AF428140 mRNA. Translation: AAQ04213.1.
    AF318348 mRNA. Translation: AAL55855.1. Frameshift.
    AC010531 Genomic DNA. No translation available.
    BC012748 mRNA. Translation: AAH12748.1. Different initiation.
    AL117444 mRNA. Translation: CAB55929.2. Different initiation.
    CCDSiCCDS32501.1. [Q5XUX0-1]
    PIRiT17239.
    RefSeqiNP_001269612.1. NM_001282683.1.
    NP_079011.3. NM_024735.4. [Q5XUX0-1]
    UniGeneiHs.567582.
    Hs.658034.
    Hs.733212.

    Genome annotation databases

    EnsembliENST00000311635; ENSP00000310841; ENSG00000103264. [Q5XUX0-1]
    GeneIDi79791.
    KEGGihsa:79791.
    UCSCiuc002fjv.3. human. [Q5XUX0-2]
    uc002fjw.3. human. [Q5XUX0-1]

    Polymorphism databases

    DMDMi146345419.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY736035 mRNA. Translation: AAU50679.1 .
    AF428140 mRNA. Translation: AAQ04213.1 .
    AF318348 mRNA. Translation: AAL55855.1 . Frameshift.
    AC010531 Genomic DNA. No translation available.
    BC012748 mRNA. Translation: AAH12748.1 . Different initiation.
    AL117444 mRNA. Translation: CAB55929.2 . Different initiation.
    CCDSi CCDS32501.1. [Q5XUX0-1 ]
    PIRi T17239.
    RefSeqi NP_001269612.1. NM_001282683.1.
    NP_079011.3. NM_024735.4. [Q5XUX0-1 ]
    UniGenei Hs.567582.
    Hs.658034.
    Hs.733212.

    3D structure databases

    ProteinModelPortali Q5XUX0.
    SMRi Q5XUX0. Positions 66-137.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 122890. 8 interactions.
    DIPi DIP-60449N.
    IntActi Q5XUX0. 1 interaction.
    STRINGi 9606.ENSP00000310841.

    PTM databases

    PhosphoSitei Q5XUX0.

    Polymorphism databases

    DMDMi 146345419.

    Proteomic databases

    MaxQBi Q5XUX0.
    PaxDbi Q5XUX0.
    PRIDEi Q5XUX0.

    Protocols and materials databases

    DNASUi 79791.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000311635 ; ENSP00000310841 ; ENSG00000103264 . [Q5XUX0-1 ]
    GeneIDi 79791.
    KEGGi hsa:79791.
    UCSCi uc002fjv.3. human. [Q5XUX0-2 ]
    uc002fjw.3. human. [Q5XUX0-1 ]

    Organism-specific databases

    CTDi 79791.
    GeneCardsi GC16M087365.
    HGNCi HGNC:16510. FBXO31.
    HPAi HPA030150.
    MIMi 609102. gene.
    neXtProti NX_Q5XUX0.
    PharmGKBi PA28042.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG43393.
    HOGENOMi HOG000112543.
    HOVERGENi HBG071549.
    InParanoidi Q5XUX0.
    KOi K10308.
    OMAi PERTPGV.
    OrthoDBi EOG7CG6ZR.
    PhylomeDBi Q5XUX0.
    TreeFami TF331818.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    ChiTaRSi FBXO31. human.
    GeneWikii FBXO31.
    GenomeRNAii 79791.
    NextBioi 69320.
    PROi Q5XUX0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q5XUX0.
    Bgeei Q5XUX0.
    CleanExi HS_FBXO31.
    Genevestigatori Q5XUX0.

    Family and domain databases

    InterProi IPR001810. F-box_dom.
    IPR026941. FBXO31.
    [Graphical view ]
    PANTHERi PTHR10706:SF126. PTHR10706:SF126. 1 hit.
    Pfami PF12937. F-box-like. 1 hit.
    [Graphical view ]
    SMARTi SM00256. FBOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81383. SSF81383. 1 hit.
    PROSITEi PS50181. FBOX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Systematic analysis and nomenclature of mammalian F-box proteins."
      Jin J., Cardozo T., Lovering R.C., Elledge S.J., Pagano M., Harper J.W.
      Genes Dev. 18:2573-2580(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "A novel F-box protein is differentially expressed in hematopoietic malignancies."
      Banham A.H., Cordell J.L., Jones M., Liggins A.P., Pulford K., Mason D.Y.
      Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Choriocarcinoma.
    3. "Large-scale cDNA transfection screening for genes related to cancer development and progression."
      Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.
      , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
      Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    4. "The sequence and analysis of duplication-rich human chromosome 16."
      Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
      , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
      Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Placenta.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 149-539 (ISOFORM 1).
      Tissue: Testis.
    7. "FBXO31 is the chromosome 16q24.3 senescence gene, a candidate breast tumor suppressor, and a component of an SCF complex."
      Kumar R., Neilsen P.M., Crawford J., McKirdy R., Lee J., Powell J.A., Saif Z., Martin J.M., Lombaerts M., Cornelisse C.J., Cleton-Jansen A.-M., Callen D.F.
      Cancer Res. 65:11304-11313(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, SUBUNIT, FUNCTION, DEVELOPMENTAL STAGE.
    8. "F-box protein FBXO31 mediates cyclin D1 degradation to induce G1 arrest after DNA damage."
      Santra M.K., Wajapeyee N., Green M.R.
      Nature 459:722-725(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN A SCF PROTEIN LIGASE COMPLEX, INDUCTION, INTERACTION WITH CCND1, PHOSPHORYLATION AT SER-278, MUTAGENESIS OF SER-278 AND SER-400.

    Entry informationi

    Entry nameiFBX31_HUMAN
    AccessioniPrimary (citable) accession number: Q5XUX0
    Secondary accession number(s): Q5K680
    , Q8WYV1, Q96D73, Q9UFV4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 15, 2005
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3