Reviewed, UniProtKB/Swiss-Prot Q5XUX0 (FBX31_HUMAN)

Names and origin

Protein names

Recommended name:
    F-box only protein 31

Gene names

Name:	FBXO31
Synonyms:	FBX14, FBX31
ORF Names:	PP2386

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	539 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. Specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. May act as a tumor suppressor.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Ref.7
Tissue specificity	Highly expressed in brain. Expressed at moderate levels in most tissues, except bone marrow. Ref.7
Developmental stage	Expression is cell-cycle regulated, and peaks at late G2 to early G1 phase (at protein level). Ref.7
Induction	By DNA damage. Increases after ultraviolet irradiation, X-ray irradiation, oxidative stress (H2O2) or addition of the chemotherapeutic DNA-damaging agents etoposide, adriamycin, cisplatin or fluorouracil.
Post-translational modification	Phosphorylation at Ser-278 by ATM following gamma-irradiation results in its stabilization.
Sequence similarities	Belongs to the FBXO31 family. Contains 1 F-box domain.
Sequence caution	The sequence AAL55855.1 differs from that shown. Reason: Frameshift at position 496.

Ontologies

Keywords
Biological process	Cell cycle DNA damage Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	G1 DNA damage checkpoint Ref.8 Inferred from mutant phenotype. Source: UniProtKB SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Ref.8 Inferred from direct assay. Source: UniProtKB cyclin catabolic process Ref.8 Inferred from mutant phenotype. Source: UniProtKB
Cellular component	SCF ubiquitin ligase complex Ref.8 Inferred from direct assay. Source: UniProtKB
Molecular function	cyclin binding Ref.8 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5XUX0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5XUX0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-113: MAVCARLCGV...DTIWRRRCRE → MFLVT

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 539	539	F-box only protein 31		PRO_0000119921
Regions
	Domain	64 – 110	47	F-box
	Compositional bias	58 – 63	6	Poly-Pro
Amino acid modifications
	Modified residue	33	1	Phosphoserine By similarity
	Modified residue	37	1	Phosphothreonine By similarity
	Modified residue	278	1	Phosphoserine; by ATM
Natural variations
	Alternative sequence	1 – 113	113	MAVCA…RRCRE → MFLVT in isoform 2.		VSP_037469
Experimental info
	Mutagenesis	278	1	S → A: Fails to accumulate following gamma-irradiation.
	Mutagenesis	400	1	S → A: No effect following gamma-irradiation.
	Sequence conflict	138	1	L → V in AAU50679. Ref.1
	Sequence conflict	281	1	D → E in AAU50679. Ref.1
	Sequence conflict	466	1	C → S in AAU50679. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2007. Version 2. Checksum: E833D63A361E7381	FASTA	539	60,664
	10 20 30 40 50 60 MAVCARLCGV GPSRGCRRRQ QRRGPAETAA ADSEPDTDPE EERIEASAGV GGGLCAGPSP 70 80 90 100 110 120 PPPRCSLLEL PPELLVEIFA SLPGTDLPSL AQVCTKFRRI LHTDTIWRRR CREEYGVCEN 130 140 150 160 170 180 LRKLEITGVS CRDVYAKLLH RYRHILGLWQ PDIGPYGGLL NVVVDGLFII GWMYLPPHDP 190 200 210 220 230 240 HVDDPMRFKP LFRIHLMERK AATVECMYGH KGPHHGHIQI VKKDEFSTKC NQTDHHRMSG 250 260 270 280 290 300 GRQEEFRTWL REEWGRTLED IFHEHMQELI LMKFIYTSQY DNCLTYRRIY LPPSRPDDLI 310 320 330 340 350 360 KPGLFKGTYG SHGLEIVMLS FHGRRARGTK ITGDPNIPAG QQTVEIDLRH RIQLPDLENQ 370 380 390 400 410 420 RNFNELSRIV LEVRERVRQE QQEGGHEAGE GRGRQGPRES QPSPAQPRAE APSKGPDGTP 430 440 450 460 470 480 GEDGGEPGDA VAAAEQPAQC GQGQPFVLPV GVSSRNEDYP RTCRMCFYGT GLIAGHGFTS 490 500 510 520 530 PERTPGVFIL FDEDRFGFVW LELKSFSLYS RVQATFRNAD APSPQAFDEM LKNIQSLTS
	Isoform 2. Checksum: 69E7352F7253F32C	FASTA	431	49,039
	10 20 30 40 50 60 MFLVTEYGVC ENLRKLEITG VSCRDVYAKL LHRYRHILGL WQPDIGPYGG LLNVVVDGLF 70 80 90 100 110 120 IIGWMYLPPH DPHVDDPMRF KPLFRIHLME RKAATVECMY GHKGPHHGHI QIVKKDEFST 130 140 150 160 170 180 KCNQTDHHRM SGGRQEEFRT WLREEWGRTL EDIFHEHMQE LILMKFIYTS QYDNCLTYRR 190 200 210 220 230 240 IYLPPSRPDD LIKPGLFKGT YGSHGLEIVM LSFHGRRARG TKITGDPNIP AGQQTVEIDL 250 260 270 280 290 300 RHRIQLPDLE NQRNFNELSR IVLEVRERVR QEQQEGGHEA GEGRGRQGPR ESQPSPAQPR 310 320 330 340 350 360 AEAPSKGPDG TPGEDGGEPG DAVAAAEQPA QCGQGQPFVL PVGVSSRNED YPRTCRMCFY 370 380 390 400 410 420 GTGLIAGHGF TSPERTPGVF ILFDEDRFGF VWLELKSFSL YSRVQATFRN ADAPSPQAFD 430 EMLKNIQSLT S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic analysis and nomenclature of mammalian F-box proteins." Jin J., Cardozo T., Lovering R.C., Elledge S.J., Pagano M., Harper J.W. Genes Dev. 18:2573-2580(2004) [PubMed: 15520277] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"A novel F-box protein is differentially expressed in hematopoietic malignancies." Banham A.H., Cordell J.L., Jones M., Liggins A.P., Pulford K., Mason D.Y. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Choriocarcinoma.
[3]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed: 15498874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]	"The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A. Nature 432:988-994(2004) [PubMed: 15616553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 149-539 (ISOFORM 1). Tissue: Testis.
[7]	"FBXO31 is the chromosome 16q24.3 senescence gene, a candidate breast tumor suppressor, and a component of an SCF complex." Kumar R., Neilsen P.M., Crawford J., McKirdy R., Lee J., Powell J.A., Saif Z., Martin J.M., Lombaerts M., Cornelisse C.J., Cleton-Jansen A.-M., Callen D.F. Cancer Res. 65:11304-11313(2005) [PubMed: 16357137] [Abstract] Cited for: TISSUE SPECIFICITY, SUBUNIT, FUNCTION, DEVELOPMENTAL STAGE.
[8]	"F-box protein FBXO31 mediates cyclin D1 degradation to induce G1 arrest after DNA damage." Santra M.K., Wajapeyee N., Green M.R. Nature 0:0-0(2009) [PubMed: 19412162] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN A SCF PROTEIN LIGASE COMPLEX, INDUCTION, INTERACTION WITH CCND1, PHOSPHORYLATION AT SER-278, MUTAGENESIS OF SER-278 AND SER-400.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY736035 mRNA. Translation: AAU50679.1. AF428140 mRNA. Translation: AAQ04213.1. AF318348 mRNA. Translation: AAL55855.1. Frameshift. AC010531 Genomic DNA. No translation available. BC012748 mRNA. Translation: AAH12748.1. Different initiation. AL117444 mRNA. Translation: CAB55929.2. Different initiation.
IPI	IPI00028347.
PIR	T17239.
RefSeq	NP_079011.3.
UniGene	Hs.567582
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q5XUX0.
Proteomic databases
PRIDE	Q5XUX0.
Genome annotation databases
Ensembl	ENSG00000103264. Homo sapiens. [Contig view]
GeneID	79791.
KEGG	hsa:79791.
Organism-specific databases
GeneCards	GC16M085920.
HGNC	HGNC:16510. FBXO31.
MIM	609102. gene.
PharmGKB	PA28042.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q5XUX0.
HOVERGEN	Q5XUX0.
OMA	Q5XUX0. MQELILM.
Gene expression databases
ArrayExpress	Q5XUX0.
Bgee	Q5XUX0.
CleanEx	HS_FBXO31.
GermOnline	ENSG00000103264. Homo sapiens.
Family and domain databases
InterPro	IPR001810. F-box. [Graphical view]
Pfam	PF00646. F-box. 1 hit. [Graphical view]
SMART	SM00256. FBOX. 1 hit. [Graphical view]
PROSITE	PS50181. FBOX. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	69320.
SOURCE	Search...

Entry information

Entry name

FBX31_HUMAN

Accession

Primary (citable) accession number: Q5XUX0
Secondary accession number(s): Q5K680 , Q8WYV1, Q96D73, Q9UFV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	May 1, 2007
Last modified:	June 16, 2009
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)