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Protein

F-box only protein 31

Gene

FBXO31

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. Specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. May act as a tumor suppressor.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • cyclin binding Source: UniProtKB
  • ubiquitin-protein transferase activity Source: Reactome

GO - Biological processi

  • anaphase-promoting complex-dependent catabolic process Source: UniProtKB
  • cellular response to DNA damage stimulus Source: UniProtKB
  • mitotic G1 DNA damage checkpoint Source: UniProtKB
  • post-translational protein modification Source: Reactome
  • protein polyubiquitination Source: Reactome
  • SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB

Keywordsi

Biological processCell cycle, DNA damage, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-8951664 Neddylation
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SIGNORiQ5XUX0
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 31
Gene namesi
Name:FBXO31
Synonyms:FBX14, FBX31
ORF Names:PP2386
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000103264.15
HGNCiHGNC:16510 FBXO31
MIMi609102 gene
neXtProtiNX_Q5XUX0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Involvement in diseasei

Mental retardation, autosomal recessive 45 (MRT45)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. MRT45 manifestations include mild to moderate intellectual disability and dysmorphic features, including coarse facies, broad nasal bridge, fleshy nares, and thick, prominent lips.
See also OMIM:615979

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi278S → A: Fails to accumulate following gamma-irradiation. 1 Publication1
Mutagenesisi400S → A: No effect following gamma-irradiation. 1 Publication1

Keywords - Diseasei

Mental retardation

Organism-specific databases

DisGeNETi79791
MalaCardsiFBXO31
MIMi615979 phenotype
OpenTargetsiENSG00000103264
Orphaneti88616 Autosomal recessive non-syndromic intellectual disability
PharmGKBiPA28042

Polymorphism and mutation databases

DMDMi146345419

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001199211 – 539F-box only protein 31Add BLAST539

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33PhosphoserineBy similarity1
Modified residuei37PhosphothreonineBy similarity1
Modified residuei278Phosphoserine; by ATM1 Publication1

Post-translational modificationi

Phosphorylation at Ser-278 by ATM following gamma-irradiation results in its stabilization.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5XUX0
MaxQBiQ5XUX0
PaxDbiQ5XUX0
PeptideAtlasiQ5XUX0
PRIDEiQ5XUX0

PTM databases

iPTMnetiQ5XUX0
PhosphoSitePlusiQ5XUX0

Expressioni

Tissue specificityi

Highly expressed in brain. Expressed at moderate levels in most tissues, except bone marrow.1 Publication

Developmental stagei

Expression is cell-cycle regulated, and peaks at late G2 to early G1 phase (at protein level).1 Publication

Inductioni

By DNA damage. Increases after UV irradiation, X-ray irradiation, oxidative stress (H2O2) or addition of the chemotherapeutic DNA-damaging agents etoposide, adriamycin, cisplatin or fluorouracil.1 Publication

Gene expression databases

BgeeiENSG00000103264
CleanExiHS_FBXO31
ExpressionAtlasiQ5XUX0 baseline and differential
GenevisibleiQ5XUX0 HS

Organism-specific databases

HPAiHPA030150

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • cyclin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi122890, 30 interactors
CORUMiQ5XUX0
DIPiDIP-60449N
IntActiQ5XUX0, 19 interactors
STRINGi9606.ENSP00000310841

Structurei

Secondary structure

1539
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi67 – 69Combined sources3
Helixi72 – 80Combined sources9
Turni84 – 86Combined sources3
Helixi87 – 91Combined sources5
Helixi95 – 100Combined sources6
Helixi104 – 115Combined sources12
Helixi121 – 126Combined sources6
Helixi131 – 137Combined sources7
Turni138 – 142Combined sources5
Helixi143 – 145Combined sources3
Beta strandi147 – 151Combined sources5
Beta strandi160 – 165Combined sources6
Beta strandi168 – 175Combined sources8
Beta strandi187 – 195Combined sources9
Beta strandi203 – 206Combined sources4
Beta strandi209 – 211Combined sources3
Beta strandi214 – 220Combined sources7
Beta strandi222 – 231Combined sources10
Helixi233 – 235Combined sources3
Helixi242 – 253Combined sources12
Helixi258 – 261Combined sources4
Helixi264 – 278Combined sources15
Beta strandi280 – 282Combined sources3
Beta strandi285 – 289Combined sources5
Beta strandi296 – 298Combined sources3
Beta strandi303 – 308Combined sources6
Helixi310 – 312Combined sources3
Beta strandi314 – 321Combined sources8
Beta strandi323 – 332Combined sources10
Beta strandi335 – 337Combined sources3
Beta strandi341 – 352Combined sources12
Helixi357 – 360Combined sources4
Helixi363 – 380Combined sources18
Beta strandi453 – 455Combined sources3
Beta strandi464 – 475Combined sources12
Turni476 – 478Combined sources3
Beta strandi479 – 492Combined sources14
Beta strandi495 – 500Combined sources6
Helixi501 – 503Combined sources3
Beta strandi505 – 511Combined sources7
Helixi524 – 536Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5VZTX-ray2.70B/D66-539[»]
5VZUX-ray2.70B/D66-539[»]
ProteinModelPortaliQ5XUX0
SMRiQ5XUX0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini64 – 110F-boxPROSITE-ProRule annotationAdd BLAST47

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi58 – 63Poly-Pro6

Sequence similaritiesi

Belongs to the FBXO31 family.Curated

Phylogenomic databases

eggNOGiENOG410IFQE Eukaryota
ENOG410XPRQ LUCA
GeneTreeiENSGT00390000001368
HOGENOMiHOG000112543
HOVERGENiHBG071549
InParanoidiQ5XUX0
KOiK10308
OMAiPQAFEEM
OrthoDBiEOG091G0N70
PhylomeDBiQ5XUX0
TreeFamiTF331818

Family and domain databases

InterProiView protein in InterPro
IPR036047 F-box-like_dom_sf
IPR001810 F-box_dom
IPR026941 FBXO31
PANTHERiPTHR10706:SF126 PTHR10706:SF126, 1 hit
PfamiView protein in Pfam
PF12937 F-box-like, 1 hit
SMARTiView protein in SMART
SM00256 FBOX, 1 hit
SUPFAMiSSF81383 SSF81383, 1 hit
PROSITEiView protein in PROSITE
PS50181 FBOX, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5XUX0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVCARLCGV GPSRGCRRRQ QRRGPAETAA ADSEPDTDPE EERIEASAGV
60 70 80 90 100
GGGLCAGPSP PPPRCSLLEL PPELLVEIFA SLPGTDLPSL AQVCTKFRRI
110 120 130 140 150
LHTDTIWRRR CREEYGVCEN LRKLEITGVS CRDVYAKLLH RYRHILGLWQ
160 170 180 190 200
PDIGPYGGLL NVVVDGLFII GWMYLPPHDP HVDDPMRFKP LFRIHLMERK
210 220 230 240 250
AATVECMYGH KGPHHGHIQI VKKDEFSTKC NQTDHHRMSG GRQEEFRTWL
260 270 280 290 300
REEWGRTLED IFHEHMQELI LMKFIYTSQY DNCLTYRRIY LPPSRPDDLI
310 320 330 340 350
KPGLFKGTYG SHGLEIVMLS FHGRRARGTK ITGDPNIPAG QQTVEIDLRH
360 370 380 390 400
RIQLPDLENQ RNFNELSRIV LEVRERVRQE QQEGGHEAGE GRGRQGPRES
410 420 430 440 450
QPSPAQPRAE APSKGPDGTP GEDGGEPGDA VAAAEQPAQC GQGQPFVLPV
460 470 480 490 500
GVSSRNEDYP RTCRMCFYGT GLIAGHGFTS PERTPGVFIL FDEDRFGFVW
510 520 530
LELKSFSLYS RVQATFRNAD APSPQAFDEM LKNIQSLTS
Length:539
Mass (Da):60,664
Last modified:May 1, 2007 - v2
Checksum:iE833D63A361E7381
GO
Isoform 2 (identifier: Q5XUX0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-113: MAVCARLCGV...DTIWRRRCRE → MFLVT

Note: No experimental confirmation available.
Show »
Length:431
Mass (Da):49,039
Checksum:i69E7352F7253F32C
GO

Sequence cautioni

The sequence AAH12748 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAL55855 differs from that shown. Reason: Frameshift at position 496.Curated
The sequence CAB55929 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti138L → V in AAU50679 (PubMed:15520277).Curated1
Sequence conflicti281D → E in AAU50679 (PubMed:15520277).Curated1
Sequence conflicti466C → S in AAU50679 (PubMed:15520277).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0374691 – 113MAVCA…RRCRE → MFLVT in isoform 2. 1 PublicationAdd BLAST113

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY736035 mRNA Translation: AAU50679.1
AF428140 mRNA Translation: AAQ04213.1
AF318348 mRNA Translation: AAL55855.1 Frameshift.
AC010531 Genomic DNA No translation available.
BC012748 mRNA Translation: AAH12748.1 Different initiation.
AL117444 mRNA Translation: CAB55929.2 Different initiation.
CCDSiCCDS32501.1 [Q5XUX0-1]
PIRiT17239
RefSeqiNP_001269612.1, NM_001282683.1
NP_079011.3, NM_024735.4 [Q5XUX0-1]
UniGeneiHs.567582
Hs.658034
Hs.733212

Genome annotation databases

EnsembliENST00000311635; ENSP00000310841; ENSG00000103264 [Q5XUX0-1]
GeneIDi79791
KEGGihsa:79791
UCSCiuc002fjw.5 human [Q5XUX0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiFBX31_HUMAN
AccessioniPrimary (citable) accession number: Q5XUX0
Secondary accession number(s): Q5K680
, Q8WYV1, Q96D73, Q9UFV4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: May 1, 2007
Last modified: May 23, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families
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