Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calcium-independent phospholipase A2-gamma

Gene

PNPLA8

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-independent phospholipase A2, which catalyzes the hydrolysis of the sn-2 position of glycerophospholipids, PtdSer and to a lower extent PtdCho. Cleaves membrane phospholipids (By similarity).By similarity

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by E-6-bromomethylene-3-1-naphthalenyl-2H-tetrahydropyran-2-one (BEL).

GO - Molecular functioni

  1. lysophospholipase activity Source: UniProtKB-EC

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-independent phospholipase A2-gamma (EC:3.1.1.5)
Alternative name(s):
Group VIB calcium-independent phospholipase A2
Intracellular membrane-associated calcium-independent phospholipase A2 gamma
Short name:
iPLA2-gamma
Patatin-like phospholipase domain-containing protein 8
Gene namesi
Name:PNPLA8
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

Endoplasmic reticulum membrane Curated; Single-pass membrane protein Curated. Golgi apparatus membrane Curated; Single-pass membrane protein Curated. Cytoplasmperinuclear region 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei483 – 50321HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. Golgi membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 786786Calcium-independent phospholipase A2-gammaPRO_0000303216Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi4 – 41N-linked (GlcNAc...)Sequence Analysis
Modified residuei740 – 7401N6-succinyllysineBy similarity

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Expressed in kidney, heart and brain.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000019643.

Structurei

3D structure databases

ProteinModelPortaliQ5XTS1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini449 – 644196PatatinAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi485 – 4895GXSXGBy similarity

Sequence similaritiesi

Contains 1 patatin domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3621.
HOGENOMiHOG000115579.
HOVERGENiHBG102100.
InParanoidiQ5XTS1.
KOiK16815.

Family and domain databases

InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002641. Patatin/PLipase_A2-rel.
[Graphical view]
PfamiPF01734. Patatin. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5XTS1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSINLTIDIC IYLLSNARNL CGKHRSKQLH LVCSPNHCWK IRHVSLQRGL
60 70 80 90 100
HPHKVRCKWT KSETHSCSKH YYSPSNHGLH IGILKLSTSA PKGLTKVSIR
110 120 130 140 150
MSRIKSTLNS VSKAVFGSQN EMISRLAQFK PSSRILRKVS DSGWLKQESI
160 170 180 190 200
KQAIRSLKKY SDKSTEKSPV PEGRNHIIDK EDDIGKQSLF HYTGNITTKF
210 220 230 240 250
GESFYFLSNH INSYFKRAEK MSQDKENSHF QEKSELEGKK VEEGKSSSLD
260 270 280 290 300
PGILTSQADK PDPKSSAGTM DKATSPSGTP ESLPISTKQS IANFLSRPTE
310 320 330 340 350
GVQALVGGYI GGLVPKLKYD SKSQAEEQEE PAKSEPAGSK DKTVEEKKHL
360 370 380 390 400
SLQREKIIAR VSIDNRTRAL VQALRRTADP KLCITRVEEL TFHLLEFPEG
410 420 430 440 450
KGVAVKERLI PCLLRLRQMK DETLQAAVRE ILALIGYVDP VKGRGIRILT
460 470 480 490 500
IDGGGTRGVV ALQTLRKLVE LTQKPVHQLF DYICGVSTGA ILAFMLGLFH
510 520 530 540 550
LPLDECEELY RKLGSDIFSQ NVIVGTVKMS WSHAFYDSQT WEKILKERMG
560 570 580 590 600
SALMIETARN PMCPKVAAVS TIVNRGSTPK AFVFRNYGHF PGSQSHYLGG
610 620 630 640 650
CQYKMWQAIR ASSAAPGYFA EYALGNDLHQ DGGLLLNNPS ALAMHECKCL
660 670 680 690 700
WPDAPLECIV SLGTGRYESD VRNNTTYTSL KTKLSNVINS ATDTEEVHIM
710 720 730 740 750
LDGLLPPDTY FRFNPVMCEN IPLDESRNEK LDQLQLEGSK YIERNEHKMK
760 770 780
KVAKILSQEK TTLQKINDWI KLKTDMYEGL PFFSKL
Length:786
Mass (Da):88,177
Last modified:November 23, 2004 - v1
Checksum:iAB130C143E718367
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY738591 mRNA. Translation: AAU85256.1.
RefSeqiNP_001164743.1. NM_001171272.1.
UniGeneiOcu.3212.

Genome annotation databases

GeneIDi100328577.
KEGGiocu:100328577.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY738591 mRNA. Translation: AAU85256.1.
RefSeqiNP_001164743.1. NM_001171272.1.
UniGeneiOcu.3212.

3D structure databases

ProteinModelPortaliQ5XTS1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000019643.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100328577.
KEGGiocu:100328577.

Organism-specific databases

CTDi50640.

Phylogenomic databases

eggNOGiCOG3621.
HOGENOMiHOG000115579.
HOVERGENiHBG102100.
InParanoidiQ5XTS1.
KOiK16815.

Family and domain databases

InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002641. Patatin/PLipase_A2-rel.
[Graphical view]
PfamiPF01734. Patatin. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, CHARACTERIZATION.
    Tissue: Heart.

Entry informationi

Entry nameiPLPL8_RABIT
AccessioniPrimary (citable) accession number: Q5XTS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: November 23, 2004
Last modified: February 4, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.