Q5XQC7 (BON3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein BONZAI 3

Gene names

Name:	BON3
Ordered Locus Names:	At1g08860
ORF Names:	F7G19.25

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	584 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Negative regulator of cell death and defense responses. Repress a number of R genes and may have effects in promoting growth and development. May function in membrane trafficking and in fusion of vesicles with plasma membrane By similarity. Ref.1 Ref.6
Subunit structure	Interacts with BAP1 and BAP2. Ref.5
Subcellular location	Cell membrane; Lipid-anchor Potential.
Tissue specificity	Expressed at an extremely low level. Ref.1
Disruption phenotype	No visible phenotype, due to partial redundancy with BON1 and BON2. Double mutant bon2-bon3 has no visible phenotype. Double mutant bon1-bon3 is seedling-lethal when grown at 22 degrees Celsius. Triple mutant bon1-bon2-bon3 is seedling-lethal at any temperature. Ref.1
Sequence similarities	Belongs to the copine family. Contains 2 C2 domains. Contains 1 VWFA domain.
Sequence caution	The sequence AAB70417.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence ABE97164.1 differs from that shown. Reason: Frameshift at position 281.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Cell membrane Membrane
Domain	Repeat
PTM	Lipoprotein Myristate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of cell death Inferred from mutant phenotype Ref.6. Source: TAIR
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Potential
	Chain	2 – 584	583	Protein BONZAI 3		PRO_0000399470
Regions
	Domain	39 – 151	113	C2 1
	Domain	180 – 285	106	C2 2
	Domain	344 – 563	220	VWFA
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5XQC7 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: C0FFBD7A478352DD	FASTA	584	64,202
	10 20 30 40 50 60 MGGCLSGDVK GGKQAIGGVQ QRPTSSTIAN NAAHNDAVDF FFRSRGQYPL FSQIELTLSA 70 80 90 100 110 120 SNLLDCDITS KSDPMAVMYL RKKDGRLEEI GRTEVILNNL NPKWIEKITV SFQFEAVQTL 130 140 150 160 170 180 VFHVYDVDTR YHNVPVKTLK LKDQDFLGEG TCVLSEIMTR QNRTLTLTLT GNVRAGVNRN 190 200 210 220 230 240 LGTLSIQAEE TVASKTVAEI NFRCVNLDNK DLFSKSDPFL RISRVVETSA AVPICRTEVV 250 260 270 280 290 300 DNNLNPMWRP VCLTMQQFGS KDTPLVIECL DFNTSGNHEL IGKTEKSVAE LERLCLQKEA 310 320 330 340 350 360 ANFVYPSLSH GRNKVLKGQL IVDRYVEKVQ YSFLDYISSG FELNFMVAVD FTASNGDPRT 370 380 390 400 410 420 PSSLHYIDPS GRLNSYQQAI MEVGEVIQFY DSDKRFPAWG FGGRTSDGSV SHAFNLNGAS 430 440 450 460 470 480 YGDEVVGVEG IMVAYASALR NVSLAGPTLF SNVVDKAAHT ASQSLSQNSP KYFVLLIITD 490 500 510 520 530 540 GVLTDMAGTV DALVRASDLP LSVLIVGVGN TDFKQMEMLD ADNGRRLESS TGRIATRDIV 550 560 570 580 QFVPMKDIHS GLVSVVQALL EELPGQFLTY VRSRKINPIG APAI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The BON/CPN gene family represses cell death and promotes cell growth in Arabidopsis." Yang S., Yang H., Grisafi P., Sanchatjate S., Fink G.R., Sun Q., Hua J. Plant J. 45:166-179(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations." Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P. Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"The Arabidopsis BAP1 and BAP2 genes are general inhibitors of programmed cell death." Yang H., Yang S., Li Y., Hua J. Plant Physiol. 145:135-146(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH BAP1 AND BAP2.
[6]	"Multiple R-like genes are negatively regulated by BON1 and BON3 in arabidopsis." Li Y., Pennington B.O., Hua J. Mol. Plant Microbe Interact. 22:840-848(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY741136 mRNA. Translation: AAU89273.1. AC000106 Genomic DNA. Translation: AAB70417.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE28360.1. DQ459165 mRNA. Translation: ABE97164.1. Frameshift.
IPI	IPI00546800.
PIR	D86220.
RefSeq	NP_172362.3. NM_100759.3.
UniGene	At.51165.
3D structure databases
ProteinModelPortal	Q5XQC7.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5XQC7. 2 interactions.
STRING	3702.AT1G08860.1-P.
Proteomic databases
PaxDb	Q5XQC7.
PRIDE	Q5XQC7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G08860.1; AT1G08860.1; AT1G08860.
GeneID	837408.
KEGG	ath:AT1G08860.
Organism-specific databases
TAIR	At1g08860.
Phylogenomic databases
eggNOG	NOG269302.
HOGENOM	HOG000220898.
InParanoid	Q5XQC7.
OMA	GWAPFKP.
PhylomeDB	Q5XQC7.
ProtClustDB	CLSN2918781.
Gene expression databases
Genevestigator	Q5XQC7.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR010734. Copine. IPR002035. VWF_A. [Graphical view]
Pfam	PF00168. C2. 2 hits. PF07002. Copine. 1 hit. [Graphical view]
SMART	SM00239. C2. 2 hits. SM00327. VWA. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 2 hits.
PROSITE	PS50004. C2. 2 hits. PS50234. VWFA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BON3_ARATH

Accession

Primary (citable) accession number: Q5XQC7
Secondary accession number(s): O04042, Q1KS96

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	November 23, 2004
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program