Q5XPT3 (LARG2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycosyltransferase-like protein LARGE2
EC=2.4.-.-

Alternative name(s):
Glycosyltransferase-like 1B

Gene names

Name:

Gyltl1b

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Glycosyltransferase which participates in glycosylation of alpha-dystroglycan. May carry out the synthesis of glycoprotein and glycosphingolipid sugar chains. Has a higher activity toward alpha-dystroglycan than LARGE. Ref.1
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity Ref.1.
Tissue specificity	Highly expressed in the testis and kidney, but weakly expressed in the heart and brain. Expressed during embryogenesis from 7 dpc. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 8 family.
Sequence caution	The sequence BAC36913.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5XPT3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5XPT3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     193-227: Missing.

Isoform 3 (identifier: Q5XPT3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     67-76: MLHVAIVCAG → EKSAAPPPDN
     77-690: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 690	690	Glycosyltransferase-like protein LARGE2		PRO_0000226812
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 29	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	30 – 690	661	Lumenal Potential
Amino acid modifications
	Glycosylation	51	1	N-linked (GlcNAc...) Potential
	Glycosylation	78	1	N-linked (GlcNAc...) Potential
	Glycosylation	202	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	67 – 76	10	MLHVAIVCAG → EKSAAPPPDN in isoform 3.		VSP_041607
	Alternative sequence	77 – 690	614	Missing in isoform 3.		VSP_041608
	Alternative sequence	193 – 227	35	Missing in isoform 2.		VSP_017489
Experimental info
	Sequence conflict	144	1	K → N in BAC36913. Ref.2
	Sequence conflict	580	1	S → R in AAH33922. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 76AE2A4E8B87930E	FASTA	690	79,514
	10 20 30 40 50 60 MLPRGRPRAM GAAVLLLLLL LVVGFFLFGR DPDYGLGTTA TLDEDPYRSR NLSASSPQLL 70 80 90 100 110 120 LPPKCEMLHV AIVCAGYNSS REIITLTKSL LFYRKNPLHL HLITDAVARN ILETLFRTWM 130 140 150 160 170 180 VPAVVVSFYD AEELKPLVSW IPNKHYSGLY GLMKLVLPSI LPPSLARVIV LDTDVTFSSD 190 200 210 220 230 240 IVELWALFDH FSDKQVVGLV ENQSDWYLGN LWKNHRPWPA LGRGFNTGVI LLWLDRLQQT 250 260 270 280 290 300 GWEQMWKVTA KRELLTLMAT SLADQDIFNA VIKEHPHLVH PLPCVWNVQL SDHTRAERCY 310 320 330 340 350 360 LEAADLKVIH WNSPKKLRVK NKHAEFFRNL HLTFLGYDGK LLRRELFGCP NQFPPGAEQL 370 380 390 400 410 420 QQALTQLDEE EPCFEFRQQQ LTVHRVHITF LPHQPPPPQP HDVTLVAQLS MDRLQMLEAL 430 440 450 460 470 480 CRHWPGPMSL ALYLTDEEAQ QFLHFVETSP VLSMRKDVAY HVVYRDGPLY PVNQLRNVAL 490 500 510 520 530 540 AQALTPYVFL SDIDFLPAYS LYDYLRASIE QLELDSRRKT ALVVPAFETL HYRFSFPNSK 550 560 570 580 590 600 AELLTLLDAG SLHTFRYHEW PQGHSSTDYS RWREAQAPYS VQWSADYEPY VVVPRDCPRY 610 620 630 640 650 660 DPRFVGFGWN KVAHIIELDA QEYEFLVLPE AFSIHLPHAP SLDISRFRSS PTYRNCLQAL 670 680 690 KEEFHQDLSR RYGSAALKYL TALQQARSRA
	Isoform 2 [UniParc]. Checksum: 8C73CC720C73B69B	FASTA	655	75,436
	10 20 30 40 50 60 MLPRGRPRAM GAAVLLLLLL LVVGFFLFGR DPDYGLGTTA TLDEDPYRSR NLSASSPQLL 70 80 90 100 110 120 LPPKCEMLHV AIVCAGYNSS REIITLTKSL LFYRKNPLHL HLITDAVARN ILETLFRTWM 130 140 150 160 170 180 VPAVVVSFYD AEELKPLVSW IPNKHYSGLY GLMKLVLPSI LPPSLARVIV LDTDVTFSSD 190 200 210 220 230 240 IVELWALFDH FSGVILLWLD RLQQTGWEQM WKVTAKRELL TLMATSLADQ DIFNAVIKEH 250 260 270 280 290 300 PHLVHPLPCV WNVQLSDHTR AERCYLEAAD LKVIHWNSPK KLRVKNKHAE FFRNLHLTFL 310 320 330 340 350 360 GYDGKLLRRE LFGCPNQFPP GAEQLQQALT QLDEEEPCFE FRQQQLTVHR VHITFLPHQP 370 380 390 400 410 420 PPPQPHDVTL VAQLSMDRLQ MLEALCRHWP GPMSLALYLT DEEAQQFLHF VETSPVLSMR 430 440 450 460 470 480 KDVAYHVVYR DGPLYPVNQL RNVALAQALT PYVFLSDIDF LPAYSLYDYL RASIEQLELD 490 500 510 520 530 540 SRRKTALVVP AFETLHYRFS FPNSKAELLT LLDAGSLHTF RYHEWPQGHS STDYSRWREA 550 560 570 580 590 600 QAPYSVQWSA DYEPYVVVPR DCPRYDPRFV GFGWNKVAHI IELDAQEYEF LVLPEAFSIH 610 620 630 640 650 LPHAPSLDIS RFRSSPTYRN CLQALKEEFH QDLSRRYGSA ALKYLTALQQ ARSRA
	Isoform 3 [UniParc]. Checksum: AB9633F7594B02F7	FASTA	76	8,209
	10 20 30 40 50 60 MLPRGRPRAM GAAVLLLLLL LVVGFFLFGR DPDYGLGTTA TLDEDPYRSR NLSASSPQLL 70 LPPKCEEKSA APPPDN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the LARGE family of putative glycosyltransferases associated with dystroglycanopathies." Grewal P.K., McLaughlan J.M., Moore C.J., Browning C.A., Hewitt J.E. Glycobiology 15:912-923(2005) [PubMed: 15958417] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: C57BL/6.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: C57BL/6J.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: Czech II. Tissue: Lung and Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY742914 mRNA. Translation: AAU95213.1. AY742915 mRNA. Translation: AAU95214.1. AK077630 mRNA. Translation: BAC36913.1. Sequence problems. AL731709 Genomic DNA. Translation: CAM20620.1. BC033922 mRNA. Translation: AAH33922.1. BC096655 mRNA. Translation: AAH96655.1.
IPI	IPI00222542. IPI00480262. IPI00480499.
RefSeq	NP_001160105.1. NM_001166633.1. NP_766258.2. NM_172670.2.
UniGene	Mm.31158.
3D structure databases
ProteinModelPortal	Q5XPT3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5XPT3.
Protein family/group databases
CAZy	GT49. Glycosyltransferase Family 49. GT8. Glycosyltransferase Family 8.
PTM databases
PhosphoSite	Q5XPT3.
Proteomic databases
PRIDE	Q5XPT3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000068586; ENSMUSP00000064128; ENSMUSG00000040434. ENSMUST00000090582; ENSMUSP00000088070; ENSMUSG00000040434.
GeneID	228366.
KEGG	mmu:228366.
UCSC	uc008kxp.2. mouse.
Organism-specific databases
CTD	120071.
MGI	MGI:2443769. Gyltl1b.
Phylogenomic databases
GeneTree	ENSGT00530000063165.
HOVERGEN	HBG052308.
OrthoDB	EOG4KKZ2K.
Gene expression databases
ArrayExpress	Q5XPT3.
Bgee	Q5XPT3.
CleanEx	MM_GYLTL1B.
Genevestigator	Q5XPT3.
GermOnline	ENSMUSG00000040434. Mus musculus.
Family and domain databases
InterPro	IPR002495. Glyco_trans_8. [Graphical view]
KO	K09668.
Pfam	PF01501. Glyco_transf_8. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	378978.
SOURCE	Search...

Entry information

Entry name

LARG2_MOUSE

Accession

Primary (citable) accession number: Q5XPT3
Secondary accession number(s): A2AHG9 , Q5XPT2, Q8BJZ8, Q8K253

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 23, 2004
Last modified:	December 14, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program