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Protein

E3 ubiquitin-protein ligase RNF123

Gene

Rnf123

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1254 – 129239RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF123 (EC:6.3.2.-)
Alternative name(s):
Kip1 ubiquitination-promoting complex protein 1
RING finger protein 123
Gene namesi
Name:Rnf123
Synonyms:Kpc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2148796. Rnf123.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 13141313E3 ubiquitin-protein ligase RNF123PRO_0000250448Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei675 – 6751PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated, leading to its degradation. Deubiquitinated by USP19, thereby stimulating CDKN1B ubiquitin-dependent degradation (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ5XPI3.
MaxQBiQ5XPI3.
PaxDbiQ5XPI3.
PRIDEiQ5XPI3.

PTM databases

iPTMnetiQ5XPI3.
PhosphoSiteiQ5XPI3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000041528.
CleanExiMM_RNF123.
ExpressionAtlasiQ5XPI3. baseline and differential.
GenevisibleiQ5XPI3. MM.

Interactioni

Subunit structurei

Component of the KPC complex composed of RNF123/KPC1 and UBAC1/KPC2. Interacts with UBAC1 and CDKN1B via its N-terminal domain (By similarity).By similarity

Protein-protein interaction databases

BioGridi220011. 12 interactions.
STRINGi10090.ENSMUSP00000124548.

Structurei

3D structure databases

ProteinModelPortaliQ5XPI3.
SMRiQ5XPI3. Positions 1244-1304.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini74 – 254181B30.2/SPRYPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1236 – 12449Poly-Ala

Sequence similaritiesi

Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1254 – 129239RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2242. Eukaryota.
KOG4692. Eukaryota.
ENOG410XQ4V. LUCA.
GeneTreeiENSGT00530000063442.
HOGENOMiHOG000154153.
HOVERGENiHBG079909.
InParanoidiQ5XPI3.
KOiK12169.
OMAiAVMMYNL.
OrthoDBiEOG091G015R.
PhylomeDBiQ5XPI3.
TreeFamiTF313546.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR003877. SPRY_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5XPI3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASKGTGMSF SRKSYRLTSD AEKSRVTGIV QEKLLSDYLY RIFSPPDRGP
60 70 80 90 100
AAATSRKPLN FHNLPEHVDQ LLQVDSEDNE SQGQVEGRLG PSTVVLDHTG
110 120 130 140 150
GFEGLLLVDD DLLGVIGHSN FGTIRSTTCV YKGKWVYEVL ISSQGLMQIG
160 170 180 190 200
WCTINCRFNQ EEGVGDTHNS YAYDGNRVRK WNVTTTNYGK AWAAGDIVSC
210 220 230 240 250
LIDLDDGTLS FCLNGVSLGT AFENLSRGLG MAYFPAISLS FKESVAFNFG
260 270 280 290 300
SRPLRYPVAG FRPLQDPPFA DLVRAQRLLG CFQAVLSVEL DPVEGRLVET
310 320 330 340 350
ESSEWQLQGQ PTVLLTLAHI FHHFAPLLRK VYLVEAVLMS FLLGVVEKGT
360 370 380 390 400
PEQAQSVVHQ ILDLLWLFME DYEVQDCLKQ LMMSLLRLYR FSPIVPDLGL
410 420 430 440 450
QIHYLRLTMS ILRHEKSRKF LLSNVLFDML RSVVFFYIKS PLRVEEAGLK
460 470 480 490 500
ELIPTTWWPH RSSRESRDGK EAREETTEER QRRRAYERGC QRLKKRIEVV
510 520 530 540 550
EELQVQILKL LLDNKDDNGG EASRYIFLTK FRKFLQENAS GRGNTPVLCP
560 570 580 590 600
PEYMVCFLHR LVSALRFYWD EYKASNPRAS FSEEAYIPPQ IFYNGKVDYF
610 620 630 640 650
DLQRLGGLLS HLRKTLKDDL ASKANIVIDP LELQAATMDD LDEDEEPAPS
660 670 680 690 700
AAQRPMQALA IGGALPLPRP GWLSSPTLGR ANRFLSTAAV SLMTPRRLLS
710 720 730 740 750
TMEKVKVRSL NVEQRTREDI EGSHWNEGLL LGRPPEEPEQ PLTENSLLEV
760 770 780 790 800
LDGTVMMYNL SVHQQLGKMV GVSDDVNEYA MALRDTEDKL RRCPKRRKDI
810 820 830 840 850
LAELTKSQKV FSEKLDHLSR RLAWVHATVY SQEKMLDIYW LLRVCLRTIE
860 870 880 890 900
HGDRTGSLFA FMPEFYLSVA INSYSALKNY FGPVHSMEEL PGYEETLTRL
910 920 930 940 950
AAILAKHFAD PRIVGTDIRD SLMQALASYV CYPHSLRAVE RIPEEQRIAM
960 970 980 990 1000
VRNLLAPYEQ RPWAQTNWIL VRLWRGCGFG YRYTRLPHLL KTKPEDANLP
1010 1020 1030 1040 1050
SLQKPCPSTL LQQHMADLLR QGSDVAPSFL NSVLNQLNWA FSEFIGMIQE
1060 1070 1080 1090 1100
IQQAAERLER NFVDSRQLKV CATCFDLSVS LLRVLEMTIT LVPEIFLDWS
1110 1120 1130 1140 1150
RPTSEMLLRR LAQLLNQVLN RVTAERNLFD RVVTLRLPGL ESVDHYPILV
1160 1170 1180 1190 1200
AVTGILVRLL VHGPTSETEQ ATSVLLADPC FQLRSICYLL GQPEPLAPGT
1210 1220 1230 1240 1250
TLPAPDRKRF SLQSYTDYIS AEELAQVEQM LAHLTAASAQ AAAASLPTNE
1260 1270 1280 1290 1300
EDLCPICYAH PISAVFQPCG HKSCKACINQ HLMNNKDCFF CKATIVSVED
1310
WDKAANTSAM SSAA
Length:1,314
Mass (Da):148,652
Last modified:November 23, 2004 - v1
Checksum:i6DB1F01109FFFD28
GO
Isoform 2 (identifier: Q5XPI3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     653-653: Q → QVWQEGQ

Show »
Length:1,320
Mass (Da):149,379
Checksum:iE050EEAE879DB3B8
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei653 – 6531Q → QVWQEGQ in isoform 2. 1 PublicationVSP_020651

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY744153 mRNA. Translation: AAU93471.1.
BC057082 mRNA. Translation: AAH57082.1.
CCDSiCCDS52922.1. [Q5XPI3-1]
CCDS81072.1. [Q5XPI3-2]
RefSeqiNP_001298081.1. NM_001311152.1. [Q5XPI3-2]
NP_115932.1. NM_032543.2. [Q5XPI3-1]
XP_006511940.1. XM_006511877.2. [Q5XPI3-2]
XP_006511941.1. XM_006511878.1. [Q5XPI3-2]
XP_006511942.1. XM_006511879.2. [Q5XPI3-2]
XP_006511945.1. XM_006511882.2. [Q5XPI3-1]
XP_011241225.1. XM_011242923.1. [Q5XPI3-2]
UniGeneiMm.379272.

Genome annotation databases

EnsembliENSMUST00000047746; ENSMUSP00000040803; ENSMUSG00000041528. [Q5XPI3-2]
ENSMUST00000160249; ENSMUSP00000124548; ENSMUSG00000041528. [Q5XPI3-1]
ENSMUST00000162355; ENSMUSP00000125745; ENSMUSG00000041528. [Q5XPI3-2]
ENSMUST00000178267; ENSMUSP00000136953; ENSMUSG00000041528. [Q5XPI3-1]
GeneIDi84585.
KEGGimmu:84585.
UCSCiuc009roi.3. mouse. [Q5XPI3-2]
uc033jmw.1. mouse. [Q5XPI3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY744153 mRNA. Translation: AAU93471.1.
BC057082 mRNA. Translation: AAH57082.1.
CCDSiCCDS52922.1. [Q5XPI3-1]
CCDS81072.1. [Q5XPI3-2]
RefSeqiNP_001298081.1. NM_001311152.1. [Q5XPI3-2]
NP_115932.1. NM_032543.2. [Q5XPI3-1]
XP_006511940.1. XM_006511877.2. [Q5XPI3-2]
XP_006511941.1. XM_006511878.1. [Q5XPI3-2]
XP_006511942.1. XM_006511879.2. [Q5XPI3-2]
XP_006511945.1. XM_006511882.2. [Q5XPI3-1]
XP_011241225.1. XM_011242923.1. [Q5XPI3-2]
UniGeneiMm.379272.

3D structure databases

ProteinModelPortaliQ5XPI3.
SMRiQ5XPI3. Positions 1244-1304.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi220011. 12 interactions.
STRINGi10090.ENSMUSP00000124548.

PTM databases

iPTMnetiQ5XPI3.
PhosphoSiteiQ5XPI3.

Proteomic databases

EPDiQ5XPI3.
MaxQBiQ5XPI3.
PaxDbiQ5XPI3.
PRIDEiQ5XPI3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047746; ENSMUSP00000040803; ENSMUSG00000041528. [Q5XPI3-2]
ENSMUST00000160249; ENSMUSP00000124548; ENSMUSG00000041528. [Q5XPI3-1]
ENSMUST00000162355; ENSMUSP00000125745; ENSMUSG00000041528. [Q5XPI3-2]
ENSMUST00000178267; ENSMUSP00000136953; ENSMUSG00000041528. [Q5XPI3-1]
GeneIDi84585.
KEGGimmu:84585.
UCSCiuc009roi.3. mouse. [Q5XPI3-2]
uc033jmw.1. mouse. [Q5XPI3-1]

Organism-specific databases

CTDi63891.
MGIiMGI:2148796. Rnf123.

Phylogenomic databases

eggNOGiKOG2242. Eukaryota.
KOG4692. Eukaryota.
ENOG410XQ4V. LUCA.
GeneTreeiENSGT00530000063442.
HOGENOMiHOG000154153.
HOVERGENiHBG079909.
InParanoidiQ5XPI3.
KOiK12169.
OMAiAVMMYNL.
OrthoDBiEOG091G015R.
PhylomeDBiQ5XPI3.
TreeFamiTF313546.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

ChiTaRSiRnf123. mouse.
PROiQ5XPI3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041528.
CleanExiMM_RNF123.
ExpressionAtlasiQ5XPI3. baseline and differential.
GenevisibleiQ5XPI3. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR013320. ConA-like_dom.
IPR003877. SPRY_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00622. SPRY. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRN123_MOUSE
AccessioniPrimary (citable) accession number: Q5XPI3
Secondary accession number(s): Q6PGE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: November 23, 2004
Last modified: September 7, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.