Q5XK84 (CARM1_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone-arginine methyltransferase CARM1
EC=2.1.1.-
EC=2.1.1.125

Alternative name(s):
Coactivator-associated arginine methyltransferase 1
Protein arginine N-methyltransferase 4

Gene names

Name:

carm1

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	602 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me) and activates transcription via chromatin remodeling By similarity.
Catalytic activity	S-adenosyl-L-methionine + arginine-[histone] = S-adenosyl-L-homocysteine + N(omega)-methyl-arginine-[histone].
Subunit structure	Homodimer By similarity.
Subcellular location	Nucleus. Cytoplasm By similarity.
Sequence similarities	Belongs to the protein arginine N-methyltransferase family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: InterPro regulation of intracellular estrogen receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	histone acetyl-lysine binding Inferred from sequence or structural similarity. Source: UniProtKB histone methyltransferase activity (H3-R17 specific) Inferred from sequence or structural similarity. Source: UniProtKB ligand-dependent nuclear receptor transcription coactivator activity Inferred from sequence or structural similarity. Source: UniProtKB protein-arginine omega-N asymmetric methyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB transcription regulatory region DNA binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 602	602	Histone-arginine methyltransferase CARM1		PRO_0000249252
Regions
	Region	470 – 602	133	Transactivation domain By similarity
Sites
	Binding site	130	1	S-adenosyl-L-methionine By similarity
	Binding site	139	1	S-adenosyl-L-methionine By similarity
	Binding site	163	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	185	1	S-adenosyl-L-methionine By similarity
	Binding site	214	1	S-adenosyl-L-methionine By similarity
	Binding site	242	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5XK84 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 4443E2E2A2280591	FASTA	602	65,959
	10 20 30 40 50 60 MAAVSVFPGV RLLSIGDANG EIQRHAEQRE LRLEARAGDI ALYNAENVCV FKCTITRDTE 70 80 90 100 110 120 CSRVGKQSFI VTLGCNSVLV QFATPADFCS FYNIIKSCRC PATERSVFSE RTEESSAVQY 130 140 150 160 170 180 FQFYGYLSQQ QNMMQDYVRT GTYQRAILQN HTDFKDKVVL DVGCGSGILS FFAVQAGARK 190 200 210 220 230 240 VYAVEASSMA QHAELLVKSN NLTDRVVVIP GKVEEISLPE QVDMIISEPM GYMLFNERML 250 260 270 280 290 300 ESYLHAKKFL KPNGNMFPTI GDVHLAPFTD EQLYMEQFTK ANFWYQPSFH GVDLSALRGA 310 320 330 340 350 360 AVDEYFKQPI VDTFDIRILM AKSVKYTVNF LDAKETDLHR IEIPFSFHML HSGLVHGLAF 370 380 390 400 410 420 WFDVAFIGSI MTVWLSTAPT EPLTHWYQVR CLLQSPIFTK AGDTLSGTVL LIANKKQSYD 430 440 450 460 470 480 ISIVAQVDQT GSKSSNLLDL KNPFFRYTGS APSPPPGSHY SSPSENMWNT GGAYTMNTGM 490 500 510 520 530 540 GMGGMPTAYD LSSVIGGSSG ISHSNLIPLG EPPAVSCPPA PLHSEPQDLT LGTSTNTGIV 550 560 570 580 590 600 NHTHSRMGSI MSTGIVQGSA GGQAASNSSS HYPVNNQFTM GGPAISMASP MSITTNTMHY GS

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC083030 mRNA. Translation: AAH83030.1.
RefSeq	NP_001088145.1. NM_001094676.1.
UniGene	Xl.29486.
3D structure databases
HSSP	HSSP built from PDB template 1OR8 based on UniProtKB Q63009.
ProteinModelPortal	Q5XK84.
SMR	Q5XK84. Positions 1-99, 112-448.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	494851.
KEGG	xla:494851.
Organism-specific databases
CTD	10498.
Xenbase	XB-GENE-865101. carm1.
Phylogenomic databases
HOVERGEN	HBG050797.
Family and domain databases
InterPro	IPR020989. Histone-Arg_MeTrfase_N. IPR007857. Skb1_MeTrfase. [Graphical view]
KO	K05931.
Pfam	PF11531. CARM1. 1 hit. PF05185. PRMT5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARM1_XENLA

Accession

Primary (citable) accession number: Q5XK84

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program