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Q5XJY4 (PARL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Presenilins-associated rhomboid-like protein, mitochondrial

EC=3.4.21.105
Alternative name(s):
Mitochondrial intramembrane-cleaving protease PARL

Cleaved into the following chain:

  1. P-beta
    Short name=Pbeta
Gene names
Name:Parl
Synonyms:Psarl
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptoptic signals By similarity. Ref.3

Catalytic activity

Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.

Subunit structure

Interacts with PSEN1 and PSEN2 By similarity. Binds OPA1. Ref.3

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein Ref.3.

P-beta: Nucleus. Note: Translocated into the nucleus by an unknown mechanism. Ref.3

Post-translational modification

P-beta is proteolytically processed (beta-cleavage) in a PARL-dependent manner By similarity.

Disruption phenotype

Mice develop normally until 4 weeks of age. They show subsequent progressive growth retardation, atrophy of muscle, spleen, and thymus as well as severe apoptosis of T and B lymphocytes leading to premature death between 8-12 weeks of age. Mouse embryonic fibroblasts lacking Parl show high susceptibility to intrinsic apoptotic signals. This defect can be complemented by Parl or a soluble form of Opa1. Ref.3

Sequence similarities

Belongs to the peptidase S54 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5050Mitochondrion By similarity
Chain51 – 377327Presenilins-associated rhomboid-like protein, mitochondrial
PRO_0000027388
Peptide51 – 7525P-beta By similarity
PRO_0000027389

Regions

Topological domain51 – 9949Mitochondrial matrix Potential
Transmembrane100 – 11920Helical; Potential
Topological domain120 – 16546Mitochondrial intermembrane Potential
Transmembrane166 – 18520Helical; Potential
Topological domain186 – 20520Mitochondrial matrix Potential
Transmembrane206 – 22823Helical; Potential
Topological domain229 – 24214Mitochondrial intermembrane Potential
Transmembrane243 – 26018Helical; Potential
Topological domain261 – 27010Mitochondrial matrix Potential
Transmembrane271 – 28717Helical; Potential
Topological domain288 – 2936Mitochondrial intermembrane Potential
Transmembrane294 – 31623Helical; Potential
Topological domain317 – 33014Mitochondrial matrix Potential
Transmembrane331 – 35222Helical; Potential
Topological domain353 – 37725Mitochondrial intermembrane Potential

Sites

Active site2751Nucleophile By similarity
Active site3331 By similarity

Amino acid modifications

Modified residue631Phosphoserine By similarity
Modified residue681Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5XJY4 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 41C81310031A2EC4

FASTA37741,964
        10         20         30         40         50         60 
MALQGWVQRG WRCGPAWAPP LGGGYRELSA TQAPRLLGRR FNLFVQQKCG FRKAPRKVEP 

        70         80         90        100        110        120 
RRSDTGSSGE AYKRSALIPP LEETVFYPSP YPIRTLVKPF FFTIGFTGCA FGSAAIWQYE 

       130        140        150        160        170        180 
SLKSRVQSYF DGIKADWLDS IRPQKEGNLR KEINKWWNSL SDGQRTVTGI IAANALVFCL 

       190        200        210        220        230        240 
WRVPSLQRTM IRYFTSNPAS KVLCSPMLLS TFSHFSLFHM AANMYVLWSF SSSIVNILGQ 

       250        260        270        280        290        300 
EQFVAVYLSA GVISNFVSYV CKVATGRYGP SLGASGAIMT VLAAVCTKIP EGRLAIIFLP 

       310        320        330        340        350        360 
VFTFTAGNAL KAIIAMDTAG MILGWKFFDH AAHLGGALFG IWYITYGHEL IWKNREPLVK 

       370 
IWHEIRTNGP KKGGGSK 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Bone marrow.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryonic stem cell.
[3]"Mitochondrial rhomboid PARL regulates cytochrome c release during apoptosis via OPA1-dependent cristae remodeling."
Cipolat S., Rudka T., Hartmann D., Costa V., Serneels L., Craessaerts K., Metzger K., Frezza C., Annaert W., D'Adamio L., Derks C., Dejaegere T., Pellegrini L., D'Hooge R., Scorrano L., De Strooper B.
Cell 126:163-175(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH OPA, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK151652 mRNA. Translation: BAE30581.1.
BC083153 mRNA. Translation: AAH83153.1.
RefSeqNP_001005767.1. NM_001005767.4.
UniGeneMm.371737.

3D structure databases

ProteinModelPortalQ5XJY4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59825N.
IntActQ5XJY4. 1 interaction.
STRING10090.ENSMUSP00000045361.

PTM databases

PhosphoSiteQ5XJY4.

Proteomic databases

PaxDbQ5XJY4.
PRIDEQ5XJY4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000048642; ENSMUSP00000045361; ENSMUSG00000033918.
GeneID381038.
KEGGmmu:381038.
UCSCuc007ypm.1. mouse.

Organism-specific databases

CTD55486.
MGIMGI:1277152. Parl.

Phylogenomic databases

eggNOGCOG0705.
GeneTreeENSGT00390000013063.
HOGENOMHOG000230670.
HOVERGENHBG082107.
InParanoidQ5XJY4.
KOK09650.
OMAHEMRTNS.
OrthoDBEOG7NGQBT.
PhylomeDBQ5XJY4.
TreeFamTF313603.

Gene expression databases

BgeeQ5XJY4.
CleanExMM_PARL.
GenevestigatorQ5XJY4.

Family and domain databases

Gene3D1.20.1540.10. 1 hit.
InterProIPR002610. Peptidase_S54_rhomboid.
IPR022764. Peptidase_S54_rhomboid_dom.
[Graphical view]
PANTHERPTHR22936. PTHR22936. 1 hit.
PfamPF01694. Rhomboid. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio401496.
PROQ5XJY4.
SOURCESearch...

Entry information

Entry namePARL_MOUSE
AccessionPrimary (citable) accession number: Q5XJY4
Secondary accession number(s): Q3U9T5, Q641T5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot