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Protein

Serine/threonine-protein kinase LMTK3

Gene

Lmtk3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein kinase which phosphorylates ESR1 (in vitro) and protects it against proteasomal degradation. May also regulate ESR1 levels indirectly via a PKC-AKT-FOXO3 pathway where it decreases the activity of PKC and the phosphorylation of AKT, thereby increasing binding of transcriptional activator FOXO3 to the ESR1 promoter and increasing ESR1 transcription (By similarity). Involved in endocytic trafficking of N-methyl-D-aspartate receptors (NMDAR) in neurons (PubMed:24760852).By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei164 – 1641ATPPROSITE-ProRule annotation
Active sitei266 – 2661Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi139 – 1479ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase LMTK3 (EC:2.7.11.1By similarity)
Alternative name(s):
Apoptosis-associated tyrosine kinase 3
Lemur tyrosine kinase 3
Gene namesi
Name:Lmtk3
Synonyms:Aatyk3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:3039582. Lmtk3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei40 – 6021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Viable and grossly normal. Mice exhibit prominent behavioral abnormalities, including locomotor hyperactivity, reduced anxiety, and decreased depression-like behavior.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi266 – 2661D → V: Significant decrease in autophosphorylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 14241404Serine/threonine-protein kinase LMTK3PRO_0000259461Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei232 – 2321PhosphoserineCombined sources
Modified residuei531 – 5311PhosphoserineCombined sources
Modified residuei535 – 5351PhosphoserineCombined sources
Modified residuei947 – 9471PhosphoserineCombined sources
Modified residuei962 – 9621PhosphoserineCombined sources
Modified residuei977 – 9771PhosphoserineCombined sources

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ5XJV6.
PaxDbiQ5XJV6.
PRIDEiQ5XJV6.

PTM databases

iPTMnetiQ5XJV6.
PhosphoSiteiQ5XJV6.

Expressioni

Tissue specificityi

Expressed in brain. Predominantly expressed in cerebral cortex, thalamus, the cerebellum and hippocampal formation (at protein level).2 Publications

Developmental stagei

Up-regulated during postnatal development, and expressed in adult stage.1 Publication

Gene expression databases

BgeeiQ5XJV6.
CleanExiMM_LMTK3.

Interactioni

Subunit structurei

Interacts with ESR1 (By similarity). Interacts with AP-2 complex subunit alpha (PubMed:24760852).By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000072388.

Structurei

3D structure databases

ProteinModelPortaliQ5XJV6.
SMRiQ5XJV6. Positions 79-405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini133 – 411279Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi416 – 842427Pro-richAdd
BLAST
Compositional biasi1117 – 1223107Pro-richAdd
BLAST
Compositional biasi1347 – 142175Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGQE. Eukaryota.
ENOG410XQFM. LUCA.
HOGENOMiHOG000170369.
HOVERGENiHBG081921.
InParanoidiQ5XJV6.
KOiK08899.
PhylomeDBiQ5XJV6.
TreeFamiTF332280.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5XJV6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAPGALILL AAVSASGCLA SPAHPDGFAL SRAPLAPPYA VVLISCSGLL
60 70 80 90 100
AFIFLLLTCL CCKRGDVRFK EFENPEGEDC SGEYTPPAEE TSSSQSLPDV
110 120 130 140 150
YILPLAEVSL PMPAPQPPHS DISTPLGLSR QHLSYLQEIG SGWFGKVILG
160 170 180 190 200
EVFSDYSPAQ VVVKELRASA GPLEQRKFIS EAQPYRSLQH PNVLQCLGVC
210 220 230 240 250
VETLPFLLIM EFCQLGDLKR YLRAQRPPEG MSPELPPRDL RTLQRMGLEI
260 270 280 290 300
ARGLAHLHSH NYVHSDLALR NCLLTSDLTV RIGDYGLAHS NYKEDYYLTP
310 320 330 340 350
ERLWVPLRWA APELLGELHG SFVLVDQSRE SNVWSLGVTL WELFEFGAQP
360 370 380 390 400
YRHLSDEEVL AFVVRQQHVK LARPRLKLPY ADYWYDILQS CWRPPAQRPS
410 420 430 440 450
ASDLQLQLTY LLSERPPRPP PPPPPPRDGP FPWPWPPSHS APRPGTLSSQ
460 470 480 490 500
FPLLDGFPGA DPDDVLTVTE SSRGLNLECL WEKARRGAGR GGGAPPWQPA
510 520 530 540 550
SAPPAPHTNP SNPFYEALST PSVLPVISAR SPSVSSEYYI RLEEHGSPPE
560 570 580 590 600
PLFPNDWDPL DPGVPGPQAP QTPSEVPQLV SETWASPLFP APRPFPAQSS
610 620 630 640 650
GSGGFLLSGW DPEGRGAGET LAGDPAEVLG EQGTAPWAEE EEEESSPGED
660 670 680 690 700
SSSLGGGPSR RGPLPCPLCS REGPCSCLPL ERGDAVAGWG DHPALGCPHP
710 720 730 740 750
PEDDSSLRAE RGSLADLPLV PPTSAPLEFL DPLMGAAAPQ YPGRGPPPAP
760 770 780 790 800
PPPPPPPRAS AEPAASPDPP SALASPGSGL SSPGPKPGDS GYETETPFSP
810 820 830 840 850
EGAFPGGGAA KEEGVPRPRA PPEPPDPGAP RPPPDPGPLP LPGSQEKPTF
860 870 880 890 900
VVQVSTEQLL MSLREDVTKN LLGDKGSTPG ETGPRKAGRS PANREKGPGP
910 920 930 940 950
NRDLTSLVSR KKVPSRSLPV NGVTVLENGK PGVPDMKEKV AENGLESPEK
960 970 980 990 1000
EERALVNGEP MSPEAGEKVL ANGVLMSPKS EEKVAENGVL RLPRNTERPP
1010 1020 1030 1040 1050
EIGPRRVPGP WEKTPETGGL APETLLDRAP APCEAALPQN GLEMAPGQLG
1060 1070 1080 1090 1100
PAPKSGNPDP GTEWRVHESG GAPRAPGAGK LDLGSGGRAL GGVGTAPAGG
1110 1120 1130 1140 1150
PASAVDAKAG WVDNSRPLPP PPQPLGAQQR RPEPVPLKAR PEVAQEEEPG
1160 1170 1180 1190 1200
VPDNRLGGDM APSVDEDPLK PERKGPEMPR LFLDLGPPQG NSEQIKAKLS
1210 1220 1230 1240 1250
RLSLALPPLT LTPFPGPGPR RPPWEGADAG AAGGEAGGAG APGPAEEDGE
1260 1270 1280 1290 1300
DEDEDEEDEE AAGSRDPGRT REAPVPVVVS SADGDTVRPL RGLLKSPRAA
1310 1320 1330 1340 1350
DEPEDSELER KRKMVSFHGD VTVYLFDQET PTNELSVQGT PEGDTEPSTP
1360 1370 1380 1390 1400
PAPPTPPHPT TPGDGFPNSD SGFGGSFEWA EDFPLLPPPG PPLCFSRFSV
1410 1420
SPALETPGPP ARAPDARPAG PVEN
Length:1,424
Mass (Da):150,891
Last modified:November 23, 2004 - v1
Checksum:i86A0BACC47909C91
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti691 – 6911D → G in BAF64834 (PubMed:17651901).Curated
Sequence conflicti811 – 8111K → E in BAF64834 (PubMed:17651901).Curated
Sequence conflicti811 – 8111K → E in AAH94377 (PubMed:15489334).Curated
Sequence conflicti941 – 9411A → E in BAF64834 (PubMed:17651901).Curated
Sequence conflicti950 – 9501K → R in BAF64834 (PubMed:17651901).Curated
Sequence conflicti1266 – 12661D → G in BAF64834 (PubMed:17651901).Curated
Sequence conflicti1287 – 12871V → A in BAF64834 (PubMed:17651901).Curated
Sequence conflicti1369 – 13691S → N in BAF64834 (PubMed:17651901).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB288873 mRNA. Translation: BAF64834.1.
BC083185 mRNA. Translation: AAH83185.1.
BC094377 mRNA. Translation: AAH94377.1.
CCDSiCCDS39961.1.
RefSeqiNP_001005511.2. NM_001005511.3.
NP_001277919.1. NM_001290990.1.
XP_006541027.1. XM_006540964.2.
UniGeneiMm.44928.
Mm.483301.

Genome annotation databases

GeneIDi381983.
KEGGimmu:381983.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB288873 mRNA. Translation: BAF64834.1.
BC083185 mRNA. Translation: AAH83185.1.
BC094377 mRNA. Translation: AAH94377.1.
CCDSiCCDS39961.1.
RefSeqiNP_001005511.2. NM_001005511.3.
NP_001277919.1. NM_001290990.1.
XP_006541027.1. XM_006540964.2.
UniGeneiMm.44928.
Mm.483301.

3D structure databases

ProteinModelPortaliQ5XJV6.
SMRiQ5XJV6. Positions 79-405.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000072388.

PTM databases

iPTMnetiQ5XJV6.
PhosphoSiteiQ5XJV6.

Proteomic databases

MaxQBiQ5XJV6.
PaxDbiQ5XJV6.
PRIDEiQ5XJV6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi381983.
KEGGimmu:381983.

Organism-specific databases

CTDi114783.
MGIiMGI:3039582. Lmtk3.

Phylogenomic databases

eggNOGiENOG410IGQE. Eukaryota.
ENOG410XQFM. LUCA.
HOGENOMiHOG000170369.
HOVERGENiHBG081921.
InParanoidiQ5XJV6.
KOiK08899.
PhylomeDBiQ5XJV6.
TreeFamiTF332280.

Miscellaneous databases

NextBioi35544856.
PROiQ5XJV6.
SOURCEiSearch...

Gene expression databases

BgeeiQ5XJV6.
CleanExiMM_LMTK3.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural and functional analysis of the apoptosis-associated tyrosine kinase (AATYK) family."
    Tomomura M., Morita N., Yoshikawa F., Konishi A., Akiyama H., Furuichi T., Kamiguchi H.
    Neuroscience 148:510-521(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, PHOSPHORYLATION, MUTAGENESIS OF ASP-266.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  3. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-947, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  4. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
    Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
    Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain cortex.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232; SER-531; SER-535; SER-947; SER-962 AND SER-977, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  6. "LMTK3 deficiency causes pronounced locomotor hyperactivity and impairs endocytic trafficking."
    Inoue T., Hoshina N., Nakazawa T., Kiyama Y., Kobayashi S., Abe T., Yamamoto T., Manabe T., Yamamoto T.
    J. Neurosci. 34:5927-5937(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH AP-2 COMPLEX SUBUNIT ALPHA, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiLMTK3_MOUSE
AccessioniPrimary (citable) accession number: Q5XJV6
Secondary accession number(s): A6BLZ0, Q52KF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 23, 2004
Last modified: January 20, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.