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Protein

RNA polymerase-associated protein LEO1

Gene

Leo1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-201722. Formation of the beta-catenin:TCF transactivating complex.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase-associated protein LEO1
Gene namesi
Name:Leo1
Synonyms:Gm185
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2685031. Leo1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 667666RNA polymerase-associated protein LEO1PRO_0000247820Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei10 – 101PhosphoserineBy similarity
Modified residuei14 – 141PhosphoserineBy similarity
Modified residuei66 – 661PhosphoserineBy similarity
Modified residuei151 – 1511PhosphoserineBy similarity
Modified residuei154 – 1541PhosphoserineBy similarity
Modified residuei162 – 1621PhosphoserineBy similarity
Modified residuei171 – 1711PhosphoserineBy similarity
Modified residuei179 – 1791PhosphoserineBy similarity
Modified residuei189 – 1891PhosphothreonineBy similarity
Modified residuei198 – 1981PhosphoserineBy similarity
Modified residuei206 – 2061PhosphoserineBy similarity
Modified residuei213 – 2131PhosphoserineBy similarity
Modified residuei221 – 2211PhosphoserineBy similarity
Modified residuei230 – 2301PhosphoserineBy similarity
Modified residuei239 – 2391PhosphoserineBy similarity
Modified residuei247 – 2471PhosphoserineBy similarity
Modified residuei255 – 2551PhosphoserineBy similarity
Modified residuei278 – 2781PhosphoserineCombined sources
Modified residuei280 – 2801PhosphoserineCombined sources
Modified residuei295 – 2951PhosphoserineBy similarity
Modified residuei297 – 2971PhosphoserineBy similarity
Modified residuei301 – 3011PhosphoserineBy similarity
Modified residuei607 – 6071PhosphotyrosineBy similarity
Modified residuei608 – 6081PhosphoserineBy similarity
Modified residuei609 – 6091PhosphoserineBy similarity
Modified residuei611 – 6111PhosphoserineCombined sources
Modified residuei615 – 6151PhosphoserineCombined sources
Modified residuei631 – 6311PhosphoserineCombined sources
Modified residuei659 – 6591PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ5XJE5.
MaxQBiQ5XJE5.
PaxDbiQ5XJE5.
PeptideAtlasiQ5XJE5.
PRIDEiQ5XJE5.

PTM databases

iPTMnetiQ5XJE5.
PhosphoSiteiQ5XJE5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000042487.
GenevisibleiQ5XJE5. MM.

Interactioni

Subunit structurei

Component of the PAF1 complex, which consists of CDC73, PAF1, LEO1, CTR9, RTF1 and WDR61. Interacts with TCEA1, SUPT5H and CTNNB1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi231671. 11 interactions.
IntActiQ5XJE5. 3 interactions.
STRINGi10090.ENSMUSP00000046905.

Structurei

3D structure databases

ProteinModelPortaliQ5XJE5.
SMRiQ5XJE5. Positions 371-463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi26 – 330305Asp-richAdd
BLAST

Sequence similaritiesi

Belongs to the LEO1 family.Curated

Phylogenomic databases

eggNOGiKOG1181. Eukaryota.
KOG2428. Eukaryota.
ENOG410XRI0. LUCA.
GeneTreeiENSGT00550000074952.
HOGENOMiHOG000253934.
HOVERGENiHBG081913.
InParanoidiQ5XJE5.
KOiK15177.
OMAiDEGSHHS.
OrthoDBiEOG091G0ILS.
TreeFamiTF321961.

Family and domain databases

InterProiIPR007149. Leo1.
[Graphical view]
PfamiPF04004. Leo1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5XJE5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADMEDLFGS EAESEAERKD SESESDSDSD QDNGASGSNA SGSESDQDDR
60 70 80 90 100
GDSGQPSNKE LFGDDSEEEG ASHHSGSDNH SERSDNRSEA SERSDHEDNE
110 120 130 140 150
PSDEDQHSGS EAHNDDDDEG HRSDEGSRHS EAEGSEKAQS DDEKWDGEDK
160 170 180 190 200
SDQSDDEKLQ NSDDEDREQG SDEDKLQNSD DDEEKMQNTD DEDRAQISDD
210 220 230 240 250
DRQQLSEEEK GNSDDEHPVA SDNDEEKQNS DDEDQPQVSD EEKMQNSDDE
260 270 280 290 300
RPQVSDEDGR RSDGEEEQDQ KSESARGSDS EDEVLRLKRK NAIPSDSEAD
310 320 330 340 350
SDTEVPKDNN GTMDLFGGAD DISSGSDGED KPPTPGQPVD ENGLPQDQQE
360 370 380 390 400
EEPIPETRIE VEIPKVNTDL GNDLYFVKLP NFLSVEPRPF DPQYYEDEFE
410 420 430 440 450
DEEMLDEEGR TRLKLKVENT IRWRIRRDEE GNEIKESNAR IVKWSDGSMS
460 470 480 490 500
LHLGNEVFDV YKAPLQGDHN HLFIRQGTGL QGQAVFKTKL TFRPHSTDSA
510 520 530 540 550
THRKMTLSLA DRCSKTQKIR ILPMAGRDPE CQRTEMIKKE EERLRASIRR
560 570 580 590 600
ESQQRRMREK QHQRGLSASY LEPDRYDEEE EGEESVSLAA IKNRYKGGIR
610 620 630 640 650
EERARIYSSD SDEGSEEDKA QRLLKAKKLN SDEEGESSGK RKAEDDDKAN
660
KKHKKYVISD EEEEEDD
Length:667
Mass (Da):75,597
Last modified:July 27, 2011 - v2
Checksum:i73A9E04DB6ACFFDB
GO
Isoform 2 (identifier: Q5XJE5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     308-324: DNNGTMDLFGGADDISS → GSPFTLYAGLLHSSLCL
     325-667: Missing.

Note: No experimental confirmation available.
Show »
Length:324
Mass (Da):35,869
Checksum:iDCDE6326BA1C6305
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti500 – 5001A → D in AAH83358 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei308 – 32417DNNGT…DDISS → GSPFTLYAGLLHSSLCL in isoform 2. 1 PublicationVSP_020054Add
BLAST
Alternative sequencei325 – 667343Missing in isoform 2. 1 PublicationVSP_020055Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC115880 Genomic DNA. No translation available.
BC082540 mRNA. Translation: AAH82540.1.
BC083358 mRNA. Translation: AAH83358.1.
CCDSiCCDS23343.1. [Q5XJE5-1]
RefSeqiNP_001034611.1. NM_001039522.1. [Q5XJE5-1]
UniGeneiMm.41508.

Genome annotation databases

EnsembliENSMUST00000048937; ENSMUSP00000046905; ENSMUSG00000042487. [Q5XJE5-1]
GeneIDi235497.
KEGGimmu:235497.
UCSCiuc009qsh.1. mouse. [Q5XJE5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC115880 Genomic DNA. No translation available.
BC082540 mRNA. Translation: AAH82540.1.
BC083358 mRNA. Translation: AAH83358.1.
CCDSiCCDS23343.1. [Q5XJE5-1]
RefSeqiNP_001034611.1. NM_001039522.1. [Q5XJE5-1]
UniGeneiMm.41508.

3D structure databases

ProteinModelPortaliQ5XJE5.
SMRiQ5XJE5. Positions 371-463.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231671. 11 interactions.
IntActiQ5XJE5. 3 interactions.
STRINGi10090.ENSMUSP00000046905.

PTM databases

iPTMnetiQ5XJE5.
PhosphoSiteiQ5XJE5.

Proteomic databases

EPDiQ5XJE5.
MaxQBiQ5XJE5.
PaxDbiQ5XJE5.
PeptideAtlasiQ5XJE5.
PRIDEiQ5XJE5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048937; ENSMUSP00000046905; ENSMUSG00000042487. [Q5XJE5-1]
GeneIDi235497.
KEGGimmu:235497.
UCSCiuc009qsh.1. mouse. [Q5XJE5-1]

Organism-specific databases

CTDi123169.
MGIiMGI:2685031. Leo1.

Phylogenomic databases

eggNOGiKOG1181. Eukaryota.
KOG2428. Eukaryota.
ENOG410XRI0. LUCA.
GeneTreeiENSGT00550000074952.
HOGENOMiHOG000253934.
HOVERGENiHBG081913.
InParanoidiQ5XJE5.
KOiK15177.
OMAiDEGSHHS.
OrthoDBiEOG091G0ILS.
TreeFamiTF321961.

Enzyme and pathway databases

ReactomeiR-MMU-201722. Formation of the beta-catenin:TCF transactivating complex.

Miscellaneous databases

PROiQ5XJE5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042487.
GenevisibleiQ5XJE5. MM.

Family and domain databases

InterProiIPR007149. Leo1.
[Graphical view]
PfamiPF04004. Leo1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEO1_MOUSE
AccessioniPrimary (citable) accession number: Q5XJE5
Secondary accession number(s): E9QPK8, Q640R1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.