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Protein

RNA polymerase-associated protein LEO1

Gene

Leo1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-112382. Formation of RNA Pol II elongation complex.
R-MMU-112387. Elongation arrest and recovery.
R-MMU-201722. Formation of the beta-catenin:TCF transactivating complex.
R-MMU-674695. RNA Polymerase II Pre-transcription Events.
R-MMU-75955. RNA Polymerase II Transcription Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase-associated protein LEO1
Gene namesi
Name:Leo1
Synonyms:Gm185
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2685031. Leo1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002478202 – 667RNA polymerase-associated protein LEO1Add BLAST666

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei10PhosphoserineBy similarity1
Modified residuei14PhosphoserineBy similarity1
Modified residuei66PhosphoserineBy similarity1
Modified residuei151PhosphoserineBy similarity1
Modified residuei154PhosphoserineBy similarity1
Modified residuei162PhosphoserineBy similarity1
Modified residuei171PhosphoserineBy similarity1
Modified residuei179PhosphoserineBy similarity1
Modified residuei189PhosphothreonineBy similarity1
Modified residuei198PhosphoserineBy similarity1
Modified residuei206PhosphoserineBy similarity1
Modified residuei213PhosphoserineBy similarity1
Modified residuei221PhosphoserineBy similarity1
Modified residuei230PhosphoserineBy similarity1
Modified residuei239PhosphoserineBy similarity1
Modified residuei247PhosphoserineBy similarity1
Modified residuei255PhosphoserineBy similarity1
Modified residuei278PhosphoserineCombined sources1
Modified residuei280PhosphoserineCombined sources1
Modified residuei295PhosphoserineBy similarity1
Modified residuei297PhosphoserineBy similarity1
Modified residuei301PhosphoserineBy similarity1
Modified residuei607PhosphotyrosineBy similarity1
Modified residuei608PhosphoserineBy similarity1
Modified residuei609PhosphoserineBy similarity1
Modified residuei611PhosphoserineCombined sources1
Modified residuei615PhosphoserineCombined sources1
Modified residuei631PhosphoserineCombined sources1
Modified residuei659PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ5XJE5.
PaxDbiQ5XJE5.
PeptideAtlasiQ5XJE5.
PRIDEiQ5XJE5.

PTM databases

iPTMnetiQ5XJE5.
PhosphoSitePlusiQ5XJE5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000042487.
GenevisibleiQ5XJE5. MM.

Interactioni

Subunit structurei

Component of the PAF1 complex, which consists of CDC73, PAF1, LEO1, CTR9, RTF1 and WDR61. Interacts with TCEA1, SUPT5H and CTNNB1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi231671. 11 interactors.
IntActiQ5XJE5. 3 interactors.
STRINGi10090.ENSMUSP00000046905.

Structurei

3D structure databases

ProteinModelPortaliQ5XJE5.
SMRiQ5XJE5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi26 – 330Asp-richAdd BLAST305

Sequence similaritiesi

Belongs to the LEO1 family.Curated

Phylogenomic databases

eggNOGiKOG1181. Eukaryota.
KOG2428. Eukaryota.
ENOG410XRI0. LUCA.
GeneTreeiENSGT00550000074952.
HOGENOMiHOG000253934.
HOVERGENiHBG081913.
InParanoidiQ5XJE5.
KOiK15177.
OMAiDEGSHHS.
OrthoDBiEOG091G0ILS.
TreeFamiTF321961.

Family and domain databases

InterProiIPR007149. Leo1.
[Graphical view]
PfamiPF04004. Leo1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5XJE5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADMEDLFGS EAESEAERKD SESESDSDSD QDNGASGSNA SGSESDQDDR
60 70 80 90 100
GDSGQPSNKE LFGDDSEEEG ASHHSGSDNH SERSDNRSEA SERSDHEDNE
110 120 130 140 150
PSDEDQHSGS EAHNDDDDEG HRSDEGSRHS EAEGSEKAQS DDEKWDGEDK
160 170 180 190 200
SDQSDDEKLQ NSDDEDREQG SDEDKLQNSD DDEEKMQNTD DEDRAQISDD
210 220 230 240 250
DRQQLSEEEK GNSDDEHPVA SDNDEEKQNS DDEDQPQVSD EEKMQNSDDE
260 270 280 290 300
RPQVSDEDGR RSDGEEEQDQ KSESARGSDS EDEVLRLKRK NAIPSDSEAD
310 320 330 340 350
SDTEVPKDNN GTMDLFGGAD DISSGSDGED KPPTPGQPVD ENGLPQDQQE
360 370 380 390 400
EEPIPETRIE VEIPKVNTDL GNDLYFVKLP NFLSVEPRPF DPQYYEDEFE
410 420 430 440 450
DEEMLDEEGR TRLKLKVENT IRWRIRRDEE GNEIKESNAR IVKWSDGSMS
460 470 480 490 500
LHLGNEVFDV YKAPLQGDHN HLFIRQGTGL QGQAVFKTKL TFRPHSTDSA
510 520 530 540 550
THRKMTLSLA DRCSKTQKIR ILPMAGRDPE CQRTEMIKKE EERLRASIRR
560 570 580 590 600
ESQQRRMREK QHQRGLSASY LEPDRYDEEE EGEESVSLAA IKNRYKGGIR
610 620 630 640 650
EERARIYSSD SDEGSEEDKA QRLLKAKKLN SDEEGESSGK RKAEDDDKAN
660
KKHKKYVISD EEEEEDD
Length:667
Mass (Da):75,597
Last modified:July 27, 2011 - v2
Checksum:i73A9E04DB6ACFFDB
GO
Isoform 2 (identifier: Q5XJE5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     308-324: DNNGTMDLFGGADDISS → GSPFTLYAGLLHSSLCL
     325-667: Missing.

Note: No experimental confirmation available.
Show »
Length:324
Mass (Da):35,869
Checksum:iDCDE6326BA1C6305
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti500A → D in AAH83358 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020054308 – 324DNNGT…DDISS → GSPFTLYAGLLHSSLCL in isoform 2. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_020055325 – 667Missing in isoform 2. 1 PublicationAdd BLAST343

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC115880 Genomic DNA. No translation available.
BC082540 mRNA. Translation: AAH82540.1.
BC083358 mRNA. Translation: AAH83358.1.
CCDSiCCDS23343.1. [Q5XJE5-1]
RefSeqiNP_001034611.1. NM_001039522.1. [Q5XJE5-1]
UniGeneiMm.41508.

Genome annotation databases

EnsembliENSMUST00000048937; ENSMUSP00000046905; ENSMUSG00000042487. [Q5XJE5-1]
GeneIDi235497.
KEGGimmu:235497.
UCSCiuc009qsh.1. mouse. [Q5XJE5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC115880 Genomic DNA. No translation available.
BC082540 mRNA. Translation: AAH82540.1.
BC083358 mRNA. Translation: AAH83358.1.
CCDSiCCDS23343.1. [Q5XJE5-1]
RefSeqiNP_001034611.1. NM_001039522.1. [Q5XJE5-1]
UniGeneiMm.41508.

3D structure databases

ProteinModelPortaliQ5XJE5.
SMRiQ5XJE5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231671. 11 interactors.
IntActiQ5XJE5. 3 interactors.
STRINGi10090.ENSMUSP00000046905.

PTM databases

iPTMnetiQ5XJE5.
PhosphoSitePlusiQ5XJE5.

Proteomic databases

EPDiQ5XJE5.
PaxDbiQ5XJE5.
PeptideAtlasiQ5XJE5.
PRIDEiQ5XJE5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048937; ENSMUSP00000046905; ENSMUSG00000042487. [Q5XJE5-1]
GeneIDi235497.
KEGGimmu:235497.
UCSCiuc009qsh.1. mouse. [Q5XJE5-1]

Organism-specific databases

CTDi123169.
MGIiMGI:2685031. Leo1.

Phylogenomic databases

eggNOGiKOG1181. Eukaryota.
KOG2428. Eukaryota.
ENOG410XRI0. LUCA.
GeneTreeiENSGT00550000074952.
HOGENOMiHOG000253934.
HOVERGENiHBG081913.
InParanoidiQ5XJE5.
KOiK15177.
OMAiDEGSHHS.
OrthoDBiEOG091G0ILS.
TreeFamiTF321961.

Enzyme and pathway databases

ReactomeiR-MMU-112382. Formation of RNA Pol II elongation complex.
R-MMU-112387. Elongation arrest and recovery.
R-MMU-201722. Formation of the beta-catenin:TCF transactivating complex.
R-MMU-674695. RNA Polymerase II Pre-transcription Events.
R-MMU-75955. RNA Polymerase II Transcription Elongation.

Miscellaneous databases

PROiQ5XJE5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042487.
GenevisibleiQ5XJE5. MM.

Family and domain databases

InterProiIPR007149. Leo1.
[Graphical view]
PfamiPF04004. Leo1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEO1_MOUSE
AccessioniPrimary (citable) accession number: Q5XJE5
Secondary accession number(s): E9QPK8, Q640R1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.