Q5XJE5 (LEO1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA polymerase-associated protein LEO1

Gene names

Name:	Leo1
Synonyms:	Gm185

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	667 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling By similarity. Ref.7
Subunit structure	Component of the PAF1 complex, which consists of CDC73, PAF1, LEO1, CTR9, RTF1 and WDR61. Interacts with TCEA1, SUPT5H and CTNNB1 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the LEO1 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Wnt signaling pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Activator
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW endodermal cell fate commitment Inferred from mutant phenotype Ref.7. Source: UniProtKB histone H2B ubiquitination Inferred from electronic annotation. Source: Compara histone monoubiquitination Inferred from electronic annotation. Source: Compara mRNA polyadenylation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of myeloid cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of mRNA 3'-end processing Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription elongation from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: UniProtKB stem cell maintenance Inferred from mutant phenotype Ref.7. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Cdc73/Paf1 complex Inferred from sequence or structural similarity. Source: UniProtKB nucleolus Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5XJE5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5XJE5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     308-324: DNNGTMDLFGGADDISS → GSPFTLYAGLLHSSLCL
     325-667: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 667	666	RNA polymerase-associated protein LEO1		PRO_0000247820
Regions
	Compositional bias	26 – 330	305	Asp-rich
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	10	1	Phosphoserine By similarity
	Modified residue	14	1	Phosphoserine By similarity
	Modified residue	151	1	Phosphoserine By similarity
	Modified residue	154	1	Phosphoserine By similarity
	Modified residue	162	1	Phosphoserine By similarity
	Modified residue	171	1	Phosphoserine By similarity
	Modified residue	179	1	Phosphoserine By similarity
	Modified residue	189	1	Phosphothreonine By similarity
	Modified residue	198	1	Phosphoserine By similarity
	Modified residue	206	1	Phosphoserine By similarity
	Modified residue	213	1	Phosphoserine By similarity
	Modified residue	221	1	Phosphoserine By similarity
	Modified residue	230	1	Phosphoserine By similarity
	Modified residue	239	1	Phosphoserine By similarity
	Modified residue	247	1	Phosphoserine Ref.6
	Modified residue	255	1	Phosphoserine Ref.6
	Modified residue	278	1	Phosphoserine Ref.5
	Modified residue	280	1	Phosphoserine Ref.5
	Modified residue	295	1	Phosphoserine Ref.6
	Modified residue	297	1	Phosphoserine Ref.6
	Modified residue	301	1	Phosphoserine By similarity
	Modified residue	608	1	Phosphoserine Ref.5
	Modified residue	609	1	Phosphoserine Ref.5
	Modified residue	611	1	Phosphoserine Ref.5
	Modified residue	615	1	Phosphoserine Ref.5
	Modified residue	631	1	Phosphoserine Ref.3 Ref.6
	Modified residue	659	1	Phosphoserine Ref.4
Natural variations
	Alternative sequence	308 – 324	17	DNNGT…DDISS → GSPFTLYAGLLHSSLCL in isoform 2.		VSP_020054
	Alternative sequence	325 – 667	343	Missing in isoform 2.		VSP_020055
Experimental info
	Sequence conflict	500	1	A → D in AAH83358. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 73A9E04DB6ACFFDB	FASTA	667	75,597
	10 20 30 40 50 60 MADMEDLFGS EAESEAERKD SESESDSDSD QDNGASGSNA SGSESDQDDR GDSGQPSNKE 70 80 90 100 110 120 LFGDDSEEEG ASHHSGSDNH SERSDNRSEA SERSDHEDNE PSDEDQHSGS EAHNDDDDEG 130 140 150 160 170 180 HRSDEGSRHS EAEGSEKAQS DDEKWDGEDK SDQSDDEKLQ NSDDEDREQG SDEDKLQNSD 190 200 210 220 230 240 DDEEKMQNTD DEDRAQISDD DRQQLSEEEK GNSDDEHPVA SDNDEEKQNS DDEDQPQVSD 250 260 270 280 290 300 EEKMQNSDDE RPQVSDEDGR RSDGEEEQDQ KSESARGSDS EDEVLRLKRK NAIPSDSEAD 310 320 330 340 350 360 SDTEVPKDNN GTMDLFGGAD DISSGSDGED KPPTPGQPVD ENGLPQDQQE EEPIPETRIE 370 380 390 400 410 420 VEIPKVNTDL GNDLYFVKLP NFLSVEPRPF DPQYYEDEFE DEEMLDEEGR TRLKLKVENT 430 440 450 460 470 480 IRWRIRRDEE GNEIKESNAR IVKWSDGSMS LHLGNEVFDV YKAPLQGDHN HLFIRQGTGL 490 500 510 520 530 540 QGQAVFKTKL TFRPHSTDSA THRKMTLSLA DRCSKTQKIR ILPMAGRDPE CQRTEMIKKE 550 560 570 580 590 600 EERLRASIRR ESQQRRMREK QHQRGLSASY LEPDRYDEEE EGEESVSLAA IKNRYKGGIR 610 620 630 640 650 660 EERARIYSSD SDEGSEEDKA QRLLKAKKLN SDEEGESSGK RKAEDDDKAN KKHKKYVISD EEEEEDD
	Isoform 2 [UniParc]. Checksum: DCDE6326BA1C6305	FASTA	324	35,869
	10 20 30 40 50 60 MADMEDLFGS EAESEAERKD SESESDSDSD QDNGASGSNA SGSESDQDDR GDSGQPSNKE 70 80 90 100 110 120 LFGDDSEEEG ASHHSGSDNH SERSDNRSEA SERSDHEDNE PSDEDQHSGS EAHNDDDDEG 130 140 150 160 170 180 HRSDEGSRHS EAEGSEKAQS DDEKWDGEDK SDQSDDEKLQ NSDDEDREQG SDEDKLQNSD 190 200 210 220 230 240 DDEEKMQNTD DEDRAQISDD DRQQLSEEEK GNSDDEHPVA SDNDEEKQNS DDEDQPQVSD 250 260 270 280 290 300 EEKMQNSDDE RPQVSDEDGR RSDGEEEQDQ KSESARGSDS EDEVLRLKRK NAIPSDSEAD 310 320 SDTEVPKGSP FTLYAGLLHS SLCL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6. Tissue: Brain.
[3]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-631, MASS SPECTROMETRY. Tissue: Brain.
[4]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-659, MASS SPECTROMETRY. Tissue: Liver.
[5]	"A differential phosphoproteomic analysis of retinoic acid-treated P19 cells." Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D. J. Proteome Res. 6:3174-3186(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-278; SER-280; SER-608; SER-609; SER-611 AND SER-615, MASS SPECTROMETRY. Tissue: Teratocarcinoma.
[6]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-247; SER-255; SER-295; SER-297 AND SER-631, MASS SPECTROMETRY. Tissue: Melanoma.
[7]	"A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity." Ding L., Paszkowski-Rogacz M., Nitzsche A., Slabicki M.M., Heninger A.K., de Vries I., Kittler R., Junqueira M., Shevchenko A., Schulz H., Hubner N., Doss M.X., Sachinidis A., Hescheler J., Iacone R., Anastassiadis K., Stewart A.F., Pisabarro M.T. , Caldarelli A., Poser I., Theis M., Buchholz F. Cell Stem Cell 4:403-415(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC115880 Genomic DNA. No translation available. BC082540 mRNA. Translation: AAH82540.1. BC083358 mRNA. Translation: AAH83358.1.
IPI	IPI00474486. IPI00776281.
RefSeq	NP_001034611.1. NM_001039522.1.
UniGene	Mm.41508.
3D structure databases
ProteinModelPortal	Q5XJE5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5XJE5. 3 interactions.
STRING	10090.ENSMUSP00000046905.
PTM databases
PhosphoSite	Q5XJE5.
Proteomic databases
PaxDb	Q5XJE5.
PRIDE	Q5XJE5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000048937; ENSMUSP00000046905; ENSMUSG00000042487.
GeneID	235497.
KEGG	mmu:235497.
UCSC	uc009qsh.1. mouse.
Organism-specific databases
CTD	123169.
MGI	MGI:2685031. Leo1.
Phylogenomic databases
eggNOG	NOG130430.
GeneTree	ENSGT00550000074952.
HOGENOM	HOG000253934.
HOVERGEN	HBG081913.
InParanoid	Q5XJE5.
KO	K15177.
OMA	NNGTMDL.
OrthoDB	EOG4HQDJC.
Gene expression databases
Bgee	Q5XJE5.
Genevestigator	Q5XJE5.
GermOnline	ENSMUSG00000042487. Mus musculus.
Family and domain databases
InterPro	IPR007149. Leo1. [Graphical view]
Pfam	PF04004. Leo1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	382712.
SOURCE	Search...

Entry information

Entry name

LEO1_MOUSE

Accession

Primary (citable) accession number: Q5XJE5
Secondary accession number(s): E9QPK8, Q640R1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program