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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

mettl1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei137 – 1371S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei140 – 1401UniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB
  2. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA (guanine-N7)-methylation Source: GO_Central
  2. tRNA methylation Source: GO_Central
  3. tRNA modification Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Methyltransferase-like protein 1UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:mettl1
ORF Names:zgc:103636
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437 Componenti: Chromosome 11

Organism-specific databases

ZFINiZDB-GENE-041010-24. mettl1.

Subcellular locationi

  1. Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB
  2. tRNA methyltransferase complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 241241tRNA (guanine-N(7)-)-methyltransferasePRO_0000370558Add
BLAST

Proteomic databases

PRIDEiQ5XJ57.

Interactioni

Subunit structurei

Forms a complex with wdr4.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni84 – 852S-adenosyl-L-methionine bindingUniRule annotation
Regioni117 – 1182S-adenosyl-L-methionine bindingUniRule annotation
Regioni215 – 2173S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
GeneTreeiENSGT00390000017840.
HOGENOMiHOG000260965.
InParanoidiQ5XJ57.
KOiK03439.
OMAiMCTHFEE.
OrthoDBiEOG7PS1GC.
TreeFamiTF314083.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5XJ57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVCMPQKRY YRQRAHSNPM ADHTFQYPVC PEQMDWSPLY PQYFPQQEEA
60 70 80 90 100
GGAQVEFADI GCGYGGLLVQ LSQLFPQQLI LGLEIRVKVS DYVQDRIRSL
110 120 130 140 150
RVAEPGRYQN IACLRSNAMK YLPNFFRKGQ LSKMFFLFPD PHFKKTKHKW
160 170 180 190 200
RIISPTLLAE YAYTLRIGGL VYTNTDVEEV HEWIVQHFSD HPLFSRVTEE
210 220 230 240
QLADDIIVGH LGTCTEEGKK VQRNGGKNFL AVFRRVEDPQ T
Length:241
Mass (Da):27,966
Last modified:October 16, 2013 - v2
Checksum:i69B0FE8B16F64E8A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731Q → L in AAI52159 (Ref. 2) Curated
Sequence conflicti189 – 1891S → G in AAH83450 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CABZ01008506 Genomic DNA. No translation available.
CABZ01061933 Genomic DNA. No translation available.
CABZ01061934 Genomic DNA. No translation available.
CU929297 Genomic DNA. No translation available.
BC083450 mRNA. Translation: AAH83450.1.
BC152158 mRNA. Translation: AAI52159.1.
RefSeqiNP_001005952.1. NM_001005952.1.
XP_005155729.1. XM_005155672.2.
XP_005155730.1. XM_005155673.2.
XP_009303993.1. XM_009305718.1.
UniGeneiDr.28333.

Genome annotation databases

EnsembliENSDART00000109091; ENSDARP00000101871; ENSDARG00000076518.
GeneIDi449779.
KEGGidre:449779.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CABZ01008506 Genomic DNA. No translation available.
CABZ01061933 Genomic DNA. No translation available.
CABZ01061934 Genomic DNA. No translation available.
CU929297 Genomic DNA. No translation available.
BC083450 mRNA. Translation: AAH83450.1.
BC152158 mRNA. Translation: AAI52159.1.
RefSeqiNP_001005952.1. NM_001005952.1.
XP_005155729.1. XM_005155672.2.
XP_005155730.1. XM_005155673.2.
XP_009303993.1. XM_009305718.1.
UniGeneiDr.28333.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ5XJ57.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000109091; ENSDARP00000101871; ENSDARG00000076518.
GeneIDi449779.
KEGGidre:449779.

Organism-specific databases

CTDi4234.
ZFINiZDB-GENE-041010-24. mettl1.

Phylogenomic databases

eggNOGiCOG0220.
GeneTreeiENSGT00390000017840.
HOGENOMiHOG000260965.
InParanoidiQ5XJ57.
KOiK03439.
OMAiMCTHFEE.
OrthoDBiEOG7PS1GC.
TreeFamiTF314083.

Enzyme and pathway databases

UniPathwayiUPA00989.

Miscellaneous databases

NextBioi20832855.
PROiQ5XJ57.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The zebrafish reference genome sequence and its relationship to the human genome."
    Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
    , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
    Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tuebingen.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Larva and Olfactory epithelium.

Entry informationi

Entry nameiTRMB_DANRE
AccessioniPrimary (citable) accession number: Q5XJ57
Secondary accession number(s): A7MCF3, F1QU80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 16, 2013
Last modified: April 29, 2015
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.