Q5XJ57 (TRMB_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
Methyltransferase-like protein 1
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	mettl1
ORF Names:	zgc:103636

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subunit structure	Forms a complex with wdr4 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	tRNA (guanine-N(7)-)-methyltransferase		PRO_0000370558
Regions
	Region	84 – 85	2	S-adenosyl-L-methionine binding By similarity
	Region	117 – 118	2	S-adenosyl-L-methionine binding By similarity
	Region	215 – 217	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	140	1	By similarity
	Binding site	61	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	137	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Experimental info
	Sequence conflict	73	1	Q → L in AAI52159. Ref.1
	Sequence conflict	189	1	G → S in AAI52159. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5XJ57 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: DDB0FE8B0D8095FC	FASTA	241	27,936
	10 20 30 40 50 60 MSVCMPQKRY YRQRAHSNPM ADHTFQYPVC PEQMDWSPLY PQYFPQQEEA GGAQVEFADI 70 80 90 100 110 120 GCGYGGLLVQ LSQLFPQQLI LGLEIRVKVS DYVQDRIRSL RVAEPGRYQN IACLRSNAMK 130 140 150 160 170 180 YLPNFFRKGQ LSKMFFLFPD PHFKKTKHKW RIISPTLLAE YAYTLRIGGL VYTNTDVEEV 190 200 210 220 230 240 HEWIVQHFGD HPLFSRVTEE QLADDIIVGH LGTCTEEGKK VQRNGGKNFL AVFRRVEDPQ T

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Larva and Olfactory epithelium.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC083450 mRNA. Translation: AAH83450.1. BC152158 mRNA. Translation: AAI52159.1.
IPI	IPI00491274.
RefSeq	NP_001005952.1. NM_001005952.1.
UniGene	Dr.28333.
3D structure databases
ProteinModelPortal	Q5XJ57.
SMR	Q5XJ57. Positions 27-239.
ModBase	Search...
Proteomic databases
PRIDE	Q5XJ57.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	449779.
KEGG	dre:449779.
Organism-specific databases
CTD	4234.
ZFIN	ZDB-GENE-041010-24. mettl1.
Phylogenomic databases
eggNOG	COG0220.
HOGENOM	HOG000260965.
KO	K03439.
OrthoDB	EOG4FJ89F.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. IPR025763. tRNA_(Gua-N-7)_MeTrfase_euk. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...
Other
NextBio	20832855.

Entry information

Entry name

TRMB_DANRE

Accession

Primary (citable) accession number: Q5XJ57
Secondary accession number(s): A7MCF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	November 23, 2004
Last modified:	April 3, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program