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Protein

Protein deglycase DJ-1zDJ-1

Gene

park7

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Protein deglycase that repairs methylglyoxal- and glyoxal-glycated amino acids and proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteines, arginines and lysines residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE). Plays an important role in cell protection against oxidative stress and cell death acting as oxidative stress sensor and redox-sensitive chaperone and protease; functions probably related to its primary function (PubMed:17166173). It is involved in neuroprotective mechanisms as well as cell growth and transformation through, for instance, the modulation of NF-kappa-B signaling pathway. Its involvement in protein repair could also explain other unrelated functions. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. Required for correct mitochondrial morphology and function as well as for autophagy of dysfunctional mitochondria. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Regulates astrocyte inflammatory responses, may modulate lipid rafts-dependent endocytosis in astrocytes and neuronal cells. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Metal-binding protein able to bind copper as well as toxic mercury ions, enhances the cell protection mechanism against induced metal toxicity.By similarity1 Publication

Cofactori

Note: Does not require glutathione as a cofactor, however, glycated glutathione constitutes a PARK7 substrate.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei106 – 1061NucleophileBy similarity
Active sitei126 – 1261By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Hydrolase, Protease

Keywords - Biological processi

Autophagy, Fertilization, Inflammatory response, Stress response

Keywords - Ligandi

Copper, RNA-binding

Protein family/group databases

MEROPSiC56.971.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein deglycase DJ-1zDJ-1By similarity (EC:3.1.2.-By similarity, EC:3.5.1.-By similarity)
Short name:
zDJ-1
Alternative name(s):
Parkinson disease protein 7 homolog
Gene namesi
Name:park7Imported
Synonyms:dj1Imported
ORF Names:zgc:103725
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 11

Organism-specific databases

ZFINiZDB-GENE-041010-5. park7.

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchor By similarity
  • Cytoplasm By similarity
  • Nucleus By similarity
  • Membrane raft By similarity
  • Mitochondrion By similarity

  • Note: Under normal conditions, located predominantly in the cytoplasm and, to a lesser extent, in the nucleus and mitochondrion. Translocates to the mitochondrion and subsequently to the nucleus in response to oxidative stress and exerts an increased cytoprotective effect against oxidative damage. Detected in tau inclusions in brains from neurodegenerative disease patients. Membrane raft localization in astrocytes and neuronal cells requires palmitoylation.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Mitochondrion, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei? – 189Removed in mature formPRO_0000405563
Chaini1 – ?Protein deglycase DJ-1zDJ-1PRO_0000285970

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi46 – 461S-palmitoyl cysteineBy similarity
Lipidationi53 – 531S-palmitoyl cysteineBy similarity
Modified residuei67 – 671PhosphotyrosineBy similarity
Modified residuei106 – 1061Cysteine sulfinic acid (-SO2H)By similarity
Modified residuei106 – 1061Cysteine sulfinic acid (-SO2H); alternateBy similarity
Lipidationi106 – 1061S-palmitoyl cysteineBy similarity
Lipidationi106 – 1061S-palmitoyl cysteine; alternateBy similarity
Cross-linki130 – 130Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei148 – 1481N6-acetyllysineBy similarity
Modified residuei182 – 1821N6-succinyllysineBy similarity

Post-translational modificationi

Sumoylated on Lys-130 by pias2 or pias4; which is essential for cell-growth promoting activity and transforming activity.By similarity
Undergoes cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Lipoprotein, Oxidation, Palmitate, Phosphoprotein, Ubl conjugation, Zymogen

Proteomic databases

PaxDbiQ5XJ36.
PRIDEiQ5XJ36.

Expressioni

Tissue specificityi

Larval brain and gut from 96 hours post-fertilization (hpf). Ubiquitous in adult; most abundant in brain, eye, heart and muscle. Within brain, neuronal expression is widespread, particularly in the cerebellum, medullary reticular formation and diencephalon. Expressed in major forebrain and diencephalic dopaminergic cell groups.2 Publications

Developmental stagei

Present from tailbud stage (10 hpf) through to adult. Levels increase from 24-120 hpf.2 Publications

Gene expression databases

BgeeiENSDARG00000034826.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000041530.

Structurei

3D structure databases

ProteinModelPortaliQ5XJ36.
SMRiQ5XJ36. Positions 1-189.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C56 family.Sequence analysis

Phylogenomic databases

eggNOGiKOG2764. Eukaryota.
COG0693. LUCA.
GeneTreeiENSGT00390000001231.
HOGENOMiHOG000063194.
HOVERGENiHBG053511.
InParanoidiQ5XJ36.
KOiK05687.
OMAiAICAAPY.
OrthoDBiEOG091G12NS.
PhylomeDBiQ5XJ36.
TreeFamiTF300119.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR002818. DJ-1/PfpI.
IPR006287. DJ1.
[Graphical view]
PfamiPF01965. DJ-1_PfpI. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01383. not_thiJ. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5XJ36-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKRALVIL AKGAEEMETV IPVDVMRRAG IAVTVAGLAG KEPVQCSREV
60 70 80 90 100
MICPDSSLED AHKQGPYDVV LLPGGLLGAQ NLSESPAVKE VLKDQEGRKG
110 120 130 140 150
LIAAICAGPT ALLAHGIAYG STVTTHPGAK DKMMAGDHYK YSEARVQKDG
160 170 180
NVITSRGPGT SFEFALTIVE ELMGAEVAAQ VKAPLILKD
Length:189
Mass (Da):19,764
Last modified:November 23, 2004 - v1
Checksum:iF1C4F9F923FB2FF1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ882651 mRNA. Translation: ABI64158.1.
BC083475 mRNA. Translation: AAH83475.1.
RefSeqiNP_001005938.1. NM_001005938.1.
UniGeneiDr.85181.

Genome annotation databases

EnsembliENSDART00000041531; ENSDARP00000041530; ENSDARG00000034826.
GeneIDi449674.
KEGGidre:449674.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ882651 mRNA. Translation: ABI64158.1.
BC083475 mRNA. Translation: AAH83475.1.
RefSeqiNP_001005938.1. NM_001005938.1.
UniGeneiDr.85181.

3D structure databases

ProteinModelPortaliQ5XJ36.
SMRiQ5XJ36. Positions 1-189.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000041530.

Protein family/group databases

MEROPSiC56.971.

Proteomic databases

PaxDbiQ5XJ36.
PRIDEiQ5XJ36.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000041531; ENSDARP00000041530; ENSDARG00000034826.
GeneIDi449674.
KEGGidre:449674.

Organism-specific databases

CTDi11315.
ZFINiZDB-GENE-041010-5. park7.

Phylogenomic databases

eggNOGiKOG2764. Eukaryota.
COG0693. LUCA.
GeneTreeiENSGT00390000001231.
HOGENOMiHOG000063194.
HOVERGENiHBG053511.
InParanoidiQ5XJ36.
KOiK05687.
OMAiAICAAPY.
OrthoDBiEOG091G12NS.
PhylomeDBiQ5XJ36.
TreeFamiTF300119.

Miscellaneous databases

PROiQ5XJ36.

Gene expression databases

BgeeiENSDARG00000034826.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR002818. DJ-1/PfpI.
IPR006287. DJ1.
[Graphical view]
PfamiPF01965. DJ-1_PfpI. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01383. not_thiJ. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPARK7_DANRE
AccessioniPrimary (citable) accession number: Q5XJ36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: November 23, 2004
Last modified: September 7, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.