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Protein

Protein artemis

Gene

Dclre1c

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation, and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ (By similarity).By similarity

GO - Molecular functioni

  1. 5'-3' exonuclease activity Source: Ensembl
  2. single-stranded DNA endodeoxyribonuclease activity Source: Ensembl

GO - Biological processi

  1. B cell differentiation Source: Ensembl
  2. double-strand break repair Source: Ensembl
  3. response to ionizing radiation Source: Ensembl
  4. telomere maintenance Source: Ensembl
  5. V(D)J recombination Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Adaptive immunity, DNA damage, DNA recombination, DNA repair, Immunity

Keywords - Ligandi

Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Protein artemis (EC:3.1.-.-)
Alternative name(s):
DNA cross-link repair 1C protein
SNM1-like protein
Gene namesi
Name:Dclre1c
Synonyms:Snm1l
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi708574. Dclre1c.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 698698Protein artemisPRO_0000209125Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei650 – 6501Phosphoserine; by ATMBy similarity

Post-translational modificationi

Phosphorylation on undefined residues by PRKDC may stimulate endonucleolytic activity on 5' and 3' hairpins and overhangs. PRKDC must remain present, even after phosphorylation, for efficient hairpin opening. Also phosphorylated by ATM in response to ionizing radiation (IR) and by ATR in response to ultraviolet (UV) radiation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ5XIX3.

PTM databases

PhosphoSiteiQ5XIX3.

Expressioni

Gene expression databases

GenevestigatoriQ5XIX3.

Interactioni

Subunit structurei

Interacts with ATM, BRCA1, PRKDC and TP53BP1. Also exhibits ATM- and phosphorylation-dependent interaction with the MRN complex, composed of MRE11A/MRE11, RAD50, and NBN (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021506.

Structurei

3D structure databases

ProteinModelPortaliQ5XIX3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1236.
GeneTreeiENSGT00530000063183.
HOGENOMiHOG000231568.
HOVERGENiHBG081421.
InParanoidiQ5XIX3.
KOiK10887.
OMAiMFRNMPD.
OrthoDBiEOG70CR8F.
PhylomeDBiQ5XIX3.
TreeFamiTF329572.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR001279. Beta-lactamas-like.
IPR011084. DRMBL.
[Graphical view]
PfamiPF07522. DRMBL. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5XIX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSFQGQMEE YPTISIDRFD RENLKARAYF LSHCHKDHMK GLRAPSMKRR
60 70 80 90 100
LECSLKVFLY CSPVTKELLL TSPKYKFWEN RIIAIEIETP TQVSLVDEAS
110 120 130 140 150
GEKEEVVVTL LPAGHCPGSV MFLFQGSNGT VLYTGDFRLA KGEVSRMELL
160 170 180 190 200
HSGGRVKDIQ SVYLDTTFCD PRFYQIPSRE ECLRGVLELV RSWITRSPKH
210 220 230 240 250
VVWLNCKAAY GYEYLFTNLS EELGVQVHVD KLDMFKNMPD ILHHLTTDRN
260 270 280 290 300
TQIHACRHPK AEEYFQWNKL PCGMASKTKT VLHTISIKPS TMWFGERTRK
310 320 330 340 350
TNVIVRTGES SYRACFSFHS SYSEIKDFLS YICPVNAYPN VIPIGLTVDK
360 370 380 390 400
VMDFLKPLCR SSQCAEPKYK PLGKLKRART VHLDSEEDDD LFDDPLLTHS
410 420 430 440 450
RRKVPYQVTL HPEVFSMKAL PLDQPELGQS PGCCKAESMP SPSLANFVDC
460 470 480 490 500
DESNSDSEGE LETPPSLQGG LGPTTLPQQN ADPDVDVPRW EVFFKRKDEI
510 520 530 540 550
TDECLENLPS SIETGGSQSP KRFSDSPKLG SDSDGESTHI SSQNSSQSTH
560 570 580 590 600
ITDQGSQGWD SQCDTVLLSS QEKSGGDSTS LNKDTYKPKP KDSISASQIE
610 620 630 640 650
QNALCPQDTH CDLKSGAEVN GVPCIEEPDT VSGRKSSPEK TSLTSTQADS
660 670 680 690
QSSSDFEIPS TPEAELPKPE HLQFLYGKLA TGESIVLKKE NVHSQIFK
Length:698
Mass (Da):78,189
Last modified:November 23, 2004 - v1
Checksum:i28D2F15EAA5ADF85
GO

Sequence cautioni

The sequence AAM89124.1 differs from that shown. Reason: Frameshift at position 686. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti623 – 6231P → S in AAM89124 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF395746 mRNA. Translation: AAM89124.1. Frameshift.
BC083546 mRNA. Translation: AAH83546.1.
RefSeqiNP_671486.2. NM_147145.2.
UniGeneiRn.204346.

Genome annotation databases

EnsembliENSRNOT00000021506; ENSRNOP00000021506; ENSRNOG00000015980.
GeneIDi259171.
KEGGirno:259171.
UCSCiRGD:708574. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF395746 mRNA. Translation: AAM89124.1. Frameshift.
BC083546 mRNA. Translation: AAH83546.1.
RefSeqiNP_671486.2. NM_147145.2.
UniGeneiRn.204346.

3D structure databases

ProteinModelPortaliQ5XIX3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021506.

PTM databases

PhosphoSiteiQ5XIX3.

Proteomic databases

PRIDEiQ5XIX3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021506; ENSRNOP00000021506; ENSRNOG00000015980.
GeneIDi259171.
KEGGirno:259171.
UCSCiRGD:708574. rat.

Organism-specific databases

CTDi64421.
RGDi708574. Dclre1c.

Phylogenomic databases

eggNOGiCOG1236.
GeneTreeiENSGT00530000063183.
HOGENOMiHOG000231568.
HOVERGENiHBG081421.
InParanoidiQ5XIX3.
KOiK10887.
OMAiMFRNMPD.
OrthoDBiEOG70CR8F.
PhylomeDBiQ5XIX3.
TreeFamiTF329572.

Miscellaneous databases

NextBioi624222.
PROiQ5XIX3.

Gene expression databases

GenevestigatoriQ5XIX3.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR001279. Beta-lactamas-like.
IPR011084. DRMBL.
[Graphical view]
PfamiPF07522. DRMBL. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse and rat SNM1-like genes, cloning, expression and mapping."
    Li L., Zhou Y., Xie G., Cowan M.J.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiDCR1C_RAT
AccessioniPrimary (citable) accession number: Q5XIX3
Secondary accession number(s): Q8K4H7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: November 23, 2004
Last modified: March 4, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.