Q5XIX3 (DCR1C_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified February 19, 2014. Version 75. History...
Names and origin
|Protein names||Recommended name:|
DNA cross-link repair 1C protein
|Organism||Rattus norvegicus (Rat) [Reference proteome]|
|Taxonomic identifier||10116 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus|
|Sequence length||698 AA.|
|Protein existence||Evidence at transcript level|
General annotation (Comments)
Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation, and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ By similarity.
Interacts with ATM, BRCA1, PRKDC and TP53BP1. Also exhibits ATM- and phosphorylation-dependent interaction with the MRN complex, composed of MRE11A/MRE11, RAD50, and NBN By similarity.
Nucleus By similarity.
Phosphorylation on undefined residues by PRKDC may stimulate endonucleolytic activity on 5' and 3' hairpins and overhangs. PRKDC must remain present, even after phosphorylation, for efficient hairpin opening. Also phosphorylated by ATM in response to ionizing radiation (IR) and by ATR in response to ultraviolet (UV) radiation By similarity.
Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.
Sequence annotation (Features)
|||"The mouse and rat SNM1-like genes, cloning, expression and mapping."|
Li L., Zhou Y., Xie G., Cowan M.J.
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
|||"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."|
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
|+||Additional computationally mapped references.|
|AF395746 mRNA. Translation: AAM89124.1. Frameshift.|
BC083546 mRNA. Translation: AAH83546.1.
|RefSeq||NP_671486.2. NM_147145.2. |
3D structure databases
Protein-protein interaction databases
Protocols and materials databases
Genome annotation databases
|Ensembl||ENSRNOT00000021506; ENSRNOP00000021506; ENSRNOG00000015980. |
|UCSC||RGD:708574. rat. |
|RGD||708574. Dclre1c. |
Gene expression databases
Family and domain databases
|Gene3D||22.214.171.124. 1 hit. |
|InterPro||IPR001279. Beta-lactamas-like. |
|Pfam||PF07522. DRMBL. 1 hit. |
|Accession||Primary (citable) accession number: Q5XIX3|
Secondary accession number(s): Q8K4H7
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
Index of protein domains and families