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Q5XIX0 (DJC14_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DnaJ homolog subfamily C member 14
Alternative name(s):
Dopamine receptor-interacting protein of 78 kDa
Short name=DRiP78
Gene names
Name:Dnajc14
Synonyms:Drip78
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length703 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates the export of target proteins, such as DRD1, from the endoplasmic reticulum to the cell surface. Ref.1

Subunit structure

Interacts with the FxxxFxxxF motif of DRD1 via its C-terminal domain. Ref.1

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.1.

Tissue specificity

Detected in heart, brain, lung, liver, skeletal muscle, kidney and testis. Ref.1

Sequence similarities

Contains 1 J domain.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentEndoplasmic reticulum
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionChaperone
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functiondopamine receptor binding

Inferred from direct assay Ref.1. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 703703DnaJ homolog subfamily C member 14
PRO_0000247495

Regions

Transmembrane254 – 27421Helical; Potential
Transmembrane305 – 32521Helical; Potential
Transmembrane327 – 34721Helical; Potential
Domain444 – 50865J
Compositional bias149 – 1524Poly-Ser

Experimental info

Mutagenesis5381C → S: Loss of interaction with DRD1. Ref.1
Sequence conflict651P → S in AAK56240. Ref.1
Sequence conflict226 – 23510RKRSQTDKRR → EVRQITPE in AAK56240. Ref.1
Sequence conflict2441E → G in AAK56240. Ref.1
Sequence conflict416 – 4183HVA → NVP in AAK56240. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q5XIX0 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 5D282F8A78DA2B08

FASTA70379,017
        10         20         30         40         50         60 
MAQKHPGERG LCGVHHSGGS SLITSGSSVD PEILSFSGLR DSKETAPNGT RCLKEHSDPK 

        70         80         90        100        110        120 
CTQPPNPAHW SDPSHGPPRG PGPPREGGYP DESETCSEES GVDQELSREN ETGYQEDGSP 

       130        140        150        160        170        180 
SFLPIPSACN CQGSPGVPEG TCSEEGDGSS SSFCHHCTSP ALGEDEELEE EYDDEEPLKF 

       190        200        210        220        230        240 
PSDFSRVSSG KKPPSRRQRH RFLTKEDVRD SGRRDPKAPG RHRLARKRSQ TDKRRGLGLW 

       250        260        270        280        290        300 
GVEELCQLGQ AGFWWLIELL VLVGEYVETC GYLIYACRKL KGSDLDLFRI WVGVWARRLG 

       310        320        330        340        350        360 
GWARVMFQFL SQSFFSVAGL FIRLLRVVGA FLLLALALFL GCLQLGWRFL VGLGDRLGWR 

       370        380        390        400        410        420 
GKAAWLFSWL DSPALHHFLT LLKDSRPWQQ LVRVIQWGWL ELPWVKQRTQ RQGTAHVASG 

       430        440        450        460        470        480 
RYCQPEEEVA RLLTMAGVPE DELNPFHVLG VEATASDIEL KKAYRQLAVM VHPDKNHHPR 

       490        500        510        520        530        540 
AEEAFKVLRA AWDIVSNPER RKEYEMKRMA ENELSRSVNE FLSKLQDDLK EAMNTMMCSR 

       550        560        570        580        590        600 
CQGKHRRFEM DREPKSARYC AECNRLHPAE EGDFWAESSM LGLKITYFAL MDGKVYDITE 

       610        620        630        640        650        660 
WAGCQRVGIS PDTHRVPYHI SFGSRVPGTS GRQRATPESP PADLQDFLSR IFQVPPGPMS 

       670        680        690        700 
NGNFFAAPHP GPGTTSTSRP NSSVPKGEAK PKRRKKVRRP FQR

« Hide

References

« Hide 'large scale' references
[1]"Regulation of transport of the dopamine D1 receptor by a new membrane-associated ER protein."
Bermak J.C., Li M., Bullock C.M., Zhou Q.-Y.
Nat. Cell Biol. 3:492-498(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH DRD1, MUTAGENESIS OF CYS-538, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF351783 mRNA. Translation: AAK56240.1.
BC083549 mRNA. Translation: AAH83549.1.
IPIIPI00391018.
RefSeqNP_446142.2. NM_053690.2.
UniGeneRn.114340.

3D structure databases

ProteinModelPortalQ5XIX0.
ModBaseSearch...

Proteomic databases

PRIDEQ5XIX0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000037332; ENSRNOP00000029946; ENSRNOG00000006844.
GeneID114481.
KEGGrno:114481.
UCSCRGD:620489. rat.

Organism-specific databases

CTD85406.
RGD620489. Dnajc14.

Phylogenomic databases

eggNOGCOG0484.
GeneTreeENSGT00670000097756.
HOGENOMHOG000290199.
HOVERGENHBG059561.
InParanoidQ5XIX0.
KOK09534.
OMAPPGQMSN.
OrthoDBEOG4RFKS1.

Gene expression databases

GenevestigatorQ5XIX0.
GermOnlineENSRNOG00000006844. Rattus norvegicus.

Family and domain databases

Gene3D1.10.287.110. 1 hit.
InterProIPR001623. DnaJ_domain.
[Graphical view]
PfamPF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSPR00625. JDOMAIN.
SMARTSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMSSF46565. DnaJ_N. 1 hit.
PROSITEPS00636. DNAJ_1. False negative.
PS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio618477.

Entry information

Entry nameDJC14_RAT
AccessionPrimary (citable) accession number: Q5XIX0
Secondary accession number(s): Q925G7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: November 23, 2004
Last modified: May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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