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Protein

U4/U6.U5 tri-snRNP-associated protein 1

Gene

Sart1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-RNO-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
U4/U6.U5 tri-snRNP-associated protein 1
Alternative name(s):
Squamous cell carcinoma antigen recognized by T-cells 1
Short name:
SART-1
Short name:
rSART-1
Gene namesi
Name:Sart1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi61930. Sart1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002233101 – 806U4/U6.U5 tri-snRNP-associated protein 1Add BLAST806

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki125Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki133Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki141Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki141Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki147Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki188Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei189PhosphothreonineBy similarity1
Cross-linki277Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei321PhosphoserineBy similarity1
Cross-linki329Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki336Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei348PhosphoserineBy similarity1
Cross-linki400Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki414Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei431PhosphothreonineBy similarity1
Modified residuei481PhosphoserineCombined sources1
Modified residuei527PhosphoserineBy similarity1
Cross-linki554Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei597PhosphoserineCombined sources1
Modified residuei602PhosphoserineCombined sources1
Modified residuei604PhosphoserineCombined sources1
Modified residuei627PhosphoserineBy similarity1
Cross-linki654Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki663Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki690Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei701PhosphothreonineBy similarity1
Cross-linki705Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki715Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki729Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki755Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki764Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei767PhosphoserineBy similarity1
Modified residuei770PhosphothreonineBy similarity1
Cross-linki781Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki786Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei795PhosphoserineBy similarity1
Cross-linki797Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Sumoylated with SUMO2.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5XIW8.
PRIDEiQ5XIW8.

PTM databases

iPTMnetiQ5XIW8.
PhosphoSitePlusiQ5XIW8.

Expressioni

Gene expression databases

BgeeiENSRNOG00000020475.
GenevisibleiQ5XIW8. RN.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027812.

Structurei

3D structure databases

SMRiQ5XIW8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili157 – 231Sequence analysisAdd BLAST75
Coiled coili495 – 540Sequence analysisAdd BLAST46

Sequence similaritiesi

Belongs to the SNU66/SART1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2217. Eukaryota.
ENOG410XSTT. LUCA.
GeneTreeiENSGT00390000007071.
HOGENOMiHOG000046291.
HOVERGENiHBG052942.
InParanoidiQ5XIW8.
KOiK11984.
OMAiARWENKQ.
OrthoDBiEOG091G04IE.
PhylomeDBiQ5XIW8.
TreeFamiTF318828.

Family and domain databases

InterProiView protein in InterPro
IPR005011. SNU66/SART1.
PANTHERiPTHR14152. PTHR14152. 1 hit.
PfamiView protein in Pfam
PF03343. SART-1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5XIW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSSKKHRGE KEAAGTTAAA GTGGTTEQPP RHREHKKHKH RSSGGGSSGG
60 70 80 90 100
ERRKRSRERG AERGSGRRGA EAEARSGAHG RERSQAEPSE RRVKREKRDE
110 120 130 140 150
GYEAAASSKA SSGDASSLSI EETNKLRAKL GLKPLEVNAV KKEAGTKEEP
160 170 180 190 200
VAADVINPMA LRQREELREK LAAAKEKRLL NQKLGKIKTL GEDDPWLDDT
210 220 230 240 250
AAWIERSRQL QKEKDLAEKR AKLLEEMDQE FGVSTLVEEE FEQRRQDLYS
260 270 280 290 300
ARDLQGLTVE HAIDSFREGE TVVLTLKDKG VLQEGEDVLV NVNMVDKERA
310 320 330 340 350
DKNVELRKKK PDYLPYAEDE SVDDLAQQKP RSILAKYDEE LEGERPHSFR
360 370 380 390 400
LEQGGMADGL RERELEEIRT KLRLQAQSLN TVGPRLASEY LSPEEMVTFK
410 420 430 440 450
KTKRRVKKIR KKEKEVIMRA DDLLPLGEDQ TQDGDFGSRL RGRGRRRVPE
460 470 480 490 500
VEEEALEDEE KDPVAQPPPS DDTRVENMDI SDEEDGGALP SGPPELEEDE
510 520 530 540 550
AELELQKQLE KGRRLRQLQQ LQQLRDSGEK VLEIVKKLES RQRGWEEEED
560 570 580 590 600
PERKGTIVFN ATSEFCRTLG EIPTYGLAGN REEQEELMDF ERDEERSANG
610 620 630 640 650
GSESDGEENI GWSTVNLDEE KQHQDFSASS TTILDEEPIV NRGLAAALLL
660 670 680 690 700
CQNKGLLETT VQKVARVKAP NKSLPSAVYC IEDKMAIDDK YSRREEYRGF
710 720 730 740 750
TQDFKEKDGY KPDVKIEYVD ETGRKLTPKE AFRQLSHRFH GKGSGKMKTE
760 770 780 790 800
RRMKKLDEEA LLKKMSSSDT PLGTVALLQE KQKAQKTPYI VLSGSGKSMN

ANTITK
Length:806
Mass (Da):91,011
Last modified:November 23, 2004 - v1
Checksum:iC8A1BCADC07544E9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti317A → V in BAA36584 (PubMed:9765622).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB014722 mRNA. Translation: BAA36584.1.
BC083551 mRNA. Translation: AAH83551.1.
RefSeqiNP_113784.1. NM_031596.1.
UniGeneiRn.25144.

Genome annotation databases

EnsembliENSRNOT00000027811; ENSRNOP00000027812; ENSRNOG00000020475.
GeneIDi29678.
KEGGirno:29678.
UCSCiRGD:61930. rat.

Similar proteinsi

Entry informationi

Entry nameiSNUT1_RAT
AccessioniPrimary (citable) accession number: Q5XIW8
Secondary accession number(s): Q9Z314
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: November 23, 2004
Last modified: August 30, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families