Reviewed, UniProtKB/Swiss-Prot Q5XIT9 (MCCB_RAT)

Names and origin

Protein names

Recommended name:
    Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial
      Short name=MCCase subunit beta
    EC=6.4.1.4
Alternative name(s):
    3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta
    3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit
    3-methylcrotonyl-CoA carboxylase 2

Gene names

Name:

Mccc2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	563 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 3-methylcrotonoyl-CoA + HCO3- = ADP + phosphate + 3-methylglutaconyl-CoA.
Pathway	Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 2/3.
Subunit structure	Probably a dodecamer composed of six biotin-containing alpha subunits and six beta subunits By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the accD/PCCB family. Contains 1 carboxyltransferase domain.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methylcrotonoyl-CoA carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion
	Chain	? – 563		Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial		PRO_0000284069
Regions
	Domain	55 – 557	503	Carboxyltransferase
	Region	343 – 372	30	Acyl-CoA binding Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5XIT9-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: B9BEB34E88B2D148	FASTA	563	61,517
	10 20 30 40 50 60 MWGALRSVLR PCSRASVPRQ RAYHGDAVAR LGTQPDSGSS TYQENYEQMK ALVNQLHERA 70 80 90 100 110 120 QYVRLGGSEK ARARHTSRGK LLPRDRIDNL IDPGSPFLEF SQFAGYKLYG EEEVPAGGII 130 140 150 160 170 180 TGIGRVSGVE CMIVANDATV KGGTYYPVTV KKHVRAQEIA LQNRLPCIYL VDSGGANLPR 190 200 210 220 230 240 QADTFPDRDH FGRIFYNQAI MSSKNITQIA VVMGSCTAGG AYVPAMADEN IIVQRQGTIF 250 260 270 280 290 300 LAGPPLVKAA TGEEVSAEDL GGADLHCRRS GVTDHYALDD HHALHLTRKV VRSLNYQKKL 310 320 330 340 350 360 DVTVEPSEEP LFPADELYGI VGANLKRSFD VREVIARIVD GSRFNEFKAL YGDTLVTGFA 370 380 390 400 410 420 RIFGYPVGII GNNGVLFSES AKKGAHFVQL CCQRNIPLLF LQNITGFMVG KDYEAEGIAK 430 440 450 460 470 480 DGAKMVAAVS CAKVPKITVI IGGSYGAGNY GMCGRAYSPR FLYMWPNARI SVMGGEQAAT 490 500 510 520 530 540 VLATVARDQR AREGKQFSSA EEAALKEPII KRFEEEGNPY YSSARLWDDG IIDPVDTRLV 550 560 LGLSISAALN APIQRTDFGI FRM

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC083581 mRNA. Translation: AAH83581.1.
IPI	IPI00192043.
RefSeq	NP_001012177.1.
UniGene	Rn.33635
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5XIT9.
Genome annotation databases
Ensembl	ENSRNOT00000023900; ENSRNOP00000023900; ENSRNOG00000017752; Rattus norvegicus. [Genome view]
GeneID	361884.
KEGG	rno:361884.
UCSC	NM_001012177. rat.
Organism-specific databases
CTD	361884.
RGD	1310279. Mccc2.
Phylogenomic databases
HOVERGEN	Q5XIT9.
Enzyme and pathway databases
BRENDA	6.4.1.4. 248.
Gene expression databases
ArrayExpress	Q5XIT9.
Genevestigator	Q5XIT9.
Family and domain databases
InterPro	IPR000022. Carboxyl_trans. IPR011763. COA_CT_C. IPR011762. COA_CT_N. [Graphical view]
Pfam	PF01039. Carboxyl_trans. 1 hit. [Graphical view]
PROSITE	PS50989. COA_CT_CTER. 1 hit. PS50980. COA_CT_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	677966.

Entry information

Entry name

MCCB_RAT

Accession

Primary (citable) accession number: Q5XIT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	November 23, 2004
Last modified:	October 13, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)