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Protein

Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial

Gene

Mccc2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Carboxyltransferase subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.By similarity

Catalytic activityi

ATP + 3-methylcrotonoyl-CoA + HCO3- = ADP + phosphate + 3-methylglutaconyl-CoA.

Pathwayi: L-leucine degradation

This protein is involved in step 2 of the subpathway that synthesizes (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Isovaleryl-CoA dehydrogenase, mitochondrial (Ivd)
  2. Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (Mccc2), Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (Mccc1)
  3. no protein annotated in this organism
This subpathway is part of the pathway L-leucine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA, the pathway L-leucine degradation and in Amino-acid degradation.

GO - Molecular functioni

GO - Biological processi

  • coenzyme A metabolic process Source: RGD
  • leucine catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-196780. Biotin transport and metabolism.
R-RNO-70895. Branched-chain amino acid catabolism.
UniPathwayiUPA00363; UER00861.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (EC:6.4.1.4)
Short name:
MCCase subunit beta
Alternative name(s):
3-methylcrotonyl-CoA carboxylase 2
3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit
3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta
Gene namesi
Name:Mccc2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1310279. Mccc2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2222MitochondrionBy similarityAdd
BLAST
Chaini23 – 563541Methylcrotonoyl-CoA carboxylase beta chain, mitochondrialPRO_0000284069Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei70 – 701N6-acetyllysine; alternateBy similarity
Modified residuei70 – 701N6-succinyllysine; alternateBy similarity
Modified residuei141 – 1411N6-succinyllysineBy similarity
Modified residuei433 – 4331N6-succinyllysineBy similarity
Modified residuei495 – 4951N6-acetyllysine; alternateBy similarity
Modified residuei495 – 4951N6-succinyllysine; alternateBy similarity
Modified residuei511 – 5111N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ5XIT9.
PRIDEiQ5XIT9.

PTM databases

PhosphoSiteiQ5XIT9.

Expressioni

Gene expression databases

GenevisibleiQ5XIT9. RN.

Interactioni

Subunit structurei

Probably a dodecamer composed of six biotin-containing alpha subunits (MCCC1) and six beta (MCCC2) subunits.By similarity

Protein-protein interaction databases

IntActiQ5XIT9. 1 interaction.
STRINGi10116.ENSRNOP00000023900.

Structurei

3D structure databases

ProteinModelPortaliQ5XIT9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 557503CarboxyltransferaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni343 – 37230Acyl-CoA bindingSequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the AccD/PCCB family.Curated
Contains 1 carboxyltransferase domain.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0540. Eukaryota.
COG4799. LUCA.
GeneTreeiENSGT00530000063337.
HOGENOMiHOG000218692.
HOVERGENiHBG052424.
InParanoidiQ5XIT9.
KOiK01969.
OMAiQCIVVAN.
OrthoDBiEOG77M8NF.
PhylomeDBiQ5XIT9.
TreeFamiTF300446.

Family and domain databases

Gene3Di3.90.226.10. 2 hits.
InterProiIPR000022. Carboxyl_trans.
IPR029045. ClpP/crotonase-like_dom.
IPR011763. COA_CT_C.
IPR011762. COA_CT_N.
[Graphical view]
PfamiPF01039. Carboxyl_trans. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 2 hits.
PROSITEiPS50989. COA_CT_CTER. 1 hit.
PS50980. COA_CT_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5XIT9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWGALRSVLR PCSRASVPRQ RAYHGDAVAR LGTQPDSGSS TYQENYEQMK
60 70 80 90 100
ALVNQLHERA QYVRLGGSEK ARARHTSRGK LLPRDRIDNL IDPGSPFLEF
110 120 130 140 150
SQFAGYKLYG EEEVPAGGII TGIGRVSGVE CMIVANDATV KGGTYYPVTV
160 170 180 190 200
KKHVRAQEIA LQNRLPCIYL VDSGGANLPR QADTFPDRDH FGRIFYNQAI
210 220 230 240 250
MSSKNITQIA VVMGSCTAGG AYVPAMADEN IIVQRQGTIF LAGPPLVKAA
260 270 280 290 300
TGEEVSAEDL GGADLHCRRS GVTDHYALDD HHALHLTRKV VRSLNYQKKL
310 320 330 340 350
DVTVEPSEEP LFPADELYGI VGANLKRSFD VREVIARIVD GSRFNEFKAL
360 370 380 390 400
YGDTLVTGFA RIFGYPVGII GNNGVLFSES AKKGAHFVQL CCQRNIPLLF
410 420 430 440 450
LQNITGFMVG KDYEAEGIAK DGAKMVAAVS CAKVPKITVI IGGSYGAGNY
460 470 480 490 500
GMCGRAYSPR FLYMWPNARI SVMGGEQAAT VLATVARDQR AREGKQFSSA
510 520 530 540 550
EEAALKEPII KRFEEEGNPY YSSARLWDDG IIDPVDTRLV LGLSISAALN
560
APIQRTDFGI FRM
Length:563
Mass (Da):61,517
Last modified:November 23, 2004 - v1
Checksum:iB9BEB34E88B2D148
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083581 mRNA. Translation: AAH83581.1.
RefSeqiNP_001012177.1. NM_001012177.1.
UniGeneiRn.33635.

Genome annotation databases

EnsembliENSRNOT00000023900; ENSRNOP00000023900; ENSRNOG00000017752.
GeneIDi361884.
KEGGirno:361884.
UCSCiRGD:1310279. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083581 mRNA. Translation: AAH83581.1.
RefSeqiNP_001012177.1. NM_001012177.1.
UniGeneiRn.33635.

3D structure databases

ProteinModelPortaliQ5XIT9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ5XIT9. 1 interaction.
STRINGi10116.ENSRNOP00000023900.

PTM databases

PhosphoSiteiQ5XIT9.

Proteomic databases

PaxDbiQ5XIT9.
PRIDEiQ5XIT9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023900; ENSRNOP00000023900; ENSRNOG00000017752.
GeneIDi361884.
KEGGirno:361884.
UCSCiRGD:1310279. rat.

Organism-specific databases

CTDi64087.
RGDi1310279. Mccc2.

Phylogenomic databases

eggNOGiKOG0540. Eukaryota.
COG4799. LUCA.
GeneTreeiENSGT00530000063337.
HOGENOMiHOG000218692.
HOVERGENiHBG052424.
InParanoidiQ5XIT9.
KOiK01969.
OMAiQCIVVAN.
OrthoDBiEOG77M8NF.
PhylomeDBiQ5XIT9.
TreeFamiTF300446.

Enzyme and pathway databases

UniPathwayiUPA00363; UER00861.
ReactomeiR-RNO-196780. Biotin transport and metabolism.
R-RNO-70895. Branched-chain amino acid catabolism.

Miscellaneous databases

NextBioi677966.
PROiQ5XIT9.

Gene expression databases

GenevisibleiQ5XIT9. RN.

Family and domain databases

Gene3Di3.90.226.10. 2 hits.
InterProiIPR000022. Carboxyl_trans.
IPR029045. ClpP/crotonase-like_dom.
IPR011763. COA_CT_C.
IPR011762. COA_CT_N.
[Graphical view]
PfamiPF01039. Carboxyl_trans. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 2 hits.
PROSITEiPS50989. COA_CT_CTER. 1 hit.
PS50980. COA_CT_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiMCCB_RAT
AccessioniPrimary (citable) accession number: Q5XIT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: November 23, 2004
Last modified: November 11, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.