Reviewed,
UniProtKB/Swiss-Prot Q5XIS9 (KPCD2_RAT)
Last modified
February 9, 2010.
Version 61.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Serine/threonine-protein kinase D2 EC=2.7.11.13 Alternative name(s): nPKC-D2 | ||
| Gene names |
| ||
| Organism | Rattus norvegicus (Rat) | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 875 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress By similarity. |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Cofactor | Magnesium By similarity. |
| Enzyme regulation | Activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-711 and phosphorylation of Ser-707 by PKC relieves auto-inhibition by the PH domain By similarity. |
| Subcellular location | Cytoplasm By similarity. Membrane By similarity. Note: Translocation to the cell membrane is required for kinase activation By similarity. |
| Post-translational modification | Phosphorylation of Ser-873 correlates with the activation status of the kinase By similarity. |
| Sequence similarities | Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily. Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm Membrane |
| Domain | Repeat Zinc-finger |
| Ligand | ATP-binding Magnesium Metal-binding Nucleotide-binding Zinc |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | intracellular signaling cascade Inferred from electronic annotation. Source: InterPro protein amino acid phosphorylationInferred from electronic annotation. Source: InterPro |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW protein kinase C activityInferred from electronic annotation. Source: EC zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 875 | 875 | Serine/threonine-protein kinase D2 | PRO_0000260437 | |||||
Regions | |||||||||
| Domain | 398 – 510 | 113 | PH | ||||||
| Domain | 552 – 808 | 257 | Protein kinase | ||||||
| Zinc finger | 138 – 188 | 51 | Phorbol-ester/DAG-type 1 | ||||||
| Zinc finger | 265 – 315 | 51 | Phorbol-ester/DAG-type 2 | ||||||
| Nucleotide binding | 558 – 566 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 675 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 581 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 189 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 197 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 198 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 199 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 200 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 203 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 206 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 211 | 1 | Phosphoserine Ref.2 | ||||||
| Modified residue | 212 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 214 | 1 | Phosphoserine Ref.2 | ||||||
| Modified residue | 225 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 227 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 236 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 237 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 238 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 339 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 354 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 363 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 375 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 376 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 397 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 519 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 707 | 1 | Phosphoserine; by PKC By similarity | ||||||
| Modified residue | 711 | 1 | Phosphoserine; by autocatalysis By similarity | ||||||
| Modified residue | 718 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 873 | 1 | Phosphoserine; by autocatalysis By similarity | ||||||
Sequences
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References
| [1] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis. |
| [2] | "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites." Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A. Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed: 16641100] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-211 AND SER-214, MASS SPECTROMETRY. Tissue: Kidney. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BC083592 mRNA. Translation: AAH83592.1. |
| IPI | IPI00364038. |
| RefSeq | NP_001013917.1. |
| UniGene | Rn.15035 |
3D structure databases | |
| HSSP | HSSP built from PDB template 2COA based on UniProtKB Q9BZL6. |
| SMR | Q5XIS9. Positions 130-192, 263-315, 396-510, 542-812. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | Q5XIS9. |
Genome annotation databases | |
| Ensembl | ENSRNOT00000022360; ENSRNOP00000022360; ENSRNOG00000016434; Rattus norvegicus. [Genome view] |
| GeneID | 292658. |
| KEGG | rno:292658. |
| NMPDR | fig|10116.3.peg.1129. |
| UCSC | NM_001013895. rat. |
Organism-specific databases | |
| CTD | 292658. |
| RGD | 1308054. Prkd2. |
Phylogenomic databases | |
| eggNOG | maNOG12873. |
| HOVERGEN | Q5XIS9. |
| InParanoid | Q5XIS9. |
| OMA | ASPWSHI. |
| OrthoDB | EOG9FXTT2. |
| PhylomeDB | Q5XIS9. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.13. 248. |
Gene expression databases | |
| ArrayExpress | Q5XIS9. |
| Genevestigator | Q5XIS9. |
| GermOnline | ENSRNOG00000016434. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR020454. DAG/PE_bd. IPR011009. Kinase-like_dom. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR015727. Protein_Kinase_C_mu-related. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view] |
| Gene3D | G3DSA:2.30.29.30. PH_type. 1 hit. |
| PANTHER | PTHR22968. PKC_mu_like. 1 hit. |
| Pfam | PF00130. C1_1. 2 hits. PF00169. PH. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view] |
| PRINTS | PR00008. DAGPEDOMAIN. |
| SMART | SM00109. C1. 2 hits. SM00233. PH. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 634460. |
Entry information
| Entry name | KPCD2_RAT | ||||||||
| Accession | Primary (citable) accession number: Q5XIS9 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

Clusters with


