SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5XIS9

- KPCD2_RAT

UniProt

Q5XIS9 - KPCD2_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Serine/threonine-protein kinase D2
Gene
Prkd2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of cell proliferation via MAPK1/3 (ERK1/2) signaling, oxidative stress-induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, signaling downstream of T-cell antigen receptor (TCR) and cytokine production, and plays a role in Golgi membrane trafficking, angiogenesis, secretory granule release and cell adhesion. May potentiate mitogenesis induced by the neuropeptide bombesin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression. In response to oxidative stress, is phosphorylated at Tyr-439 by ABL1, which leads to the activation of PRKD2 without increasing its catalytic activity, and mediates activation of NF-kappa-B. In response to the activation of the gastrin receptor CCKBR, is phosphorylated at Ser-244 by CSNK1D and CSNK1E, translocates to the nucleus, phosphorylates HDAC7, leading to nuclear export of HDAC7 and inhibition of HDAC7 transcriptional repression of NR4A1/NUR77. Upon TCR stimulation, is activated independently of ZAP70, translocates from the cytoplasm to the nucleus and is required for interleukin-2 (IL2) promoter up-regulation. During adaptive immune responses, is required in peripheral T-lymphocytes for the production of the effector cytokines IL2 and IFNG after TCR engagement and for optimal induction of antibody responses to antigens. In epithelial cells stimulated with lysophosphatidic acid (LPA), is activated through a PKC-dependent pathway and mediates LPA-stimulated interleukin-8 (IL8) secretion via a NF-kappa-B-dependent pathway. During TCR-induced T-cell activation, interacts with and is activated by the tyrosine kinase LCK, which results in the activation of the NFAT transcription factors. In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane and in polarized cells is involved in the transport of proteins from the TGN to the basolateral membrane. Plays an important role in endothelial cell proliferation and migration prior to angiogenesis, partly through modulation of the expression of KDR/VEGFR2 and FGFR1, two key growth factor receptors involved in angiogenesis. In secretory pathway, is required for the release of chromogranin-A (CHGA)-containing secretory granules from the TGN. Downstream of PRKCA, plays important roles in angiotensin-2-induced monocyte adhesion to endothelial cells By similarity.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium By similarity.

Enzyme regulationi

Activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-711 and phosphorylation of Ser-707 by PKC relieves auto-inhibition by the PH domain By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei581 – 5811ATP By similarity
Active sitei675 – 6751Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri138 – 18851Phorbol-ester/DAG-type 1
Add
BLAST
Zinc fingeri265 – 31551Phorbol-ester/DAG-type 2
Add
BLAST
Nucleotide bindingi558 – 5669ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. protein kinase C activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. T cell receptor signaling pathway Source: Ensembl
  2. angiogenesis Source: UniProtKB-KW
  3. cell adhesion Source: UniProtKB-KW
  4. cell death Source: Ensembl
  5. intracellular signal transduction Source: InterPro
  6. peptidyl-serine phosphorylation Source: Ensembl
  7. positive regulation of CREB transcription factor activity Source: Ensembl
  8. positive regulation of DNA biosynthetic process Source: UniProtKB
  9. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  10. positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
  11. positive regulation of T cell receptor signaling pathway Source: UniProtKB
  12. positive regulation of angiogenesis Source: UniProtKB
  13. positive regulation of blood vessel endothelial cell migration Source: Ensembl
  14. positive regulation of cell adhesion Source: UniProtKB
  15. positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway Source: Ensembl
  16. positive regulation of endothelial cell migration Source: UniProtKB
  17. positive regulation of endothelial cell proliferation Source: UniProtKB
  18. positive regulation of fibroblast growth factor receptor signaling pathway Source: UniProtKB
  19. positive regulation of histone deacetylase activity Source: Ensembl
  20. positive regulation of interleukin-2 production Source: UniProtKB
  21. positive regulation of interleukin-8 production Source: UniProtKB
  22. positive regulation of peptidyl-serine phosphorylation Source: Ensembl
  23. positive regulation of sequence-specific DNA binding transcription factor activity Source: UniProtKB
  24. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  25. positive regulation of vascular endothelial growth factor receptor signaling pathway Source: UniProtKB
  26. protein autophosphorylation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Adaptive immunity, Angiogenesis, Cell adhesion, Immunity

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase D2 (EC:2.7.11.13)
Alternative name(s):
nPKC-D2
Gene namesi
Name:Prkd2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi1308054. Prkd2.

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity. Golgi apparatustrans-Golgi network By similarity
Note: Translocation to the cell membrane is required for kinase activation. Accumulates in the nucleus upon CK1-mediated phosphorylation after activation of G-protein-coupled receptors. Nuclear accumulation is regulated by blocking nuclear export of active PRKD2 rather than by increasing import By similarity.

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-SubCell
  2. nucleus Source: Ensembl
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 875875Serine/threonine-protein kinase D2
PRO_0000260437Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei200 – 2001Phosphoserine By similarity
Modified residuei203 – 2031Phosphoserine By similarity
Modified residuei206 – 2061Phosphoserine By similarity
Modified residuei214 – 2141Phosphoserine By similarity
Modified residuei244 – 2441Phosphoserine; by CSNK1D and CSNK1E By similarity
Modified residuei439 – 4391Phosphotyrosine; by ABL1 By similarity
Modified residuei519 – 5191Phosphoserine By similarity
Modified residuei707 – 7071Phosphoserine; by PKC By similarity
Modified residuei711 – 7111Phosphoserine; by autocatalysis By similarity
Modified residuei718 – 7181Phosphotyrosine By similarity
Modified residuei873 – 8731Phosphoserine; by autocatalysis By similarity

Post-translational modificationi

Phosphorylation of Ser-873 correlates with the activation status of the kinase. Ser-707 is probably phosphorylated by PKC. Phosphorylation at Ser-244 by CSNK1D and CSNK1E promotes nuclear localization and substrate targeting. Phosphorylation at Ser-244, Ser-707 and Ser-711 is required for nuclear localization. Phosphorylated at Tyr-438 by ABL1 in response to oxidative stress By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5XIS9.

PTM databases

PhosphoSiteiQ5XIS9.

Expressioni

Gene expression databases

GenevestigatoriQ5XIS9.

Interactioni

Subunit structurei

Interacts (via C-terminus) with LCK By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022360.

Structurei

3D structure databases

ProteinModelPortaliQ5XIS9.
SMRiQ5XIS9. Positions 396-510.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini398 – 510113PH
Add
BLAST
Domaini552 – 808257Protein kinase
Add
BLAST

Sequence similaritiesi

Contains 1 PH domain.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00750000117523.
HOGENOMiHOG000015135.
HOVERGENiHBG003564.
InParanoidiQ5XIS9.
KOiK06070.
OMAiLPQDHEM.
OrthoDBiEOG75B84N.
PhylomeDBiQ5XIS9.
TreeFamiTF314320.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR015727. Protein_Kinase_C_mu-related.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR22968. PTHR22968. 1 hit.
PfamiPF00130. C1_1. 2 hits.
PF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000552. PKC_mu_nu_D2. 1 hit.
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00233. PH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5XIS9-1 [UniParc]FASTAAdd to Basket

« Hide

MAAAPSHPAG LPCSPGPGSP PPPGGSDLQS LPPLLPQIPA PGSGVSFHIQ    50
IGLTREFVLL PAASELAHVK QLACSIVDQK FPECGFYGLY DKILLFKHDP 100
TSANLLQLVR SAADIQEGDL VEVVLSASAT FEDFQIRPHA LTVHSYRAPA 150
FCDHCGEMLF GLVRQGLKCD GCGLNYHKRC AFSIPNNCSG ARKRRLSSTS 200
LASGHSVRLG SSESLPCTAE ELSRSTTDLL PRRPPSSSSS SSSSSFYTGR 250
PIELDKMLMS KVKVPHTFLI HSYTRPTVCQ ACKKLLKGLF RQGLQCKDCK 300
FNCHKRCATR VPNDCLGEAL INGDVPMEEA ADYSEADKSS LSDELEDSGV 350
IPGSHAENAL HASEEEEGEG GKAQSSLGYI PLMRVVQSVR HTTRKSSTTL 400
REGWVVHYSN KDTLRKRHYW RLDCKCITLF QNNTTNRYYK EIPLSEILAV 450
EPAQNFSLVP PGTNPHCFEI ITANVTYFVG ETPGGAPGGP SGQGTEAARG 500
WETAIRQALM PVILQDAPSA PGHTPHRQAS LSISVSNSQI QENVDIATVY 550
QIFPDEVLGS GQFGVVYGGK HRKTGRDVAV KVIDKLRFPT KQESQLRNEV 600
AILQSLRHPG IVNLECMFET PEKVFVVMEK LHGDMLEMIL SSEKGRLPER 650
LTKFLITQIL VALRHLHFKN IVHCDLKPEN VLLASADPFP QVKLCDFGFA 700
RIIGEKSFRR SVVGTPAYLA PEVLLNQGYN RSLDMWSVGV IMYVSLSGTF 750
PFNEDEDIND QIQNAAFMYP ASPWSHISSG AIDLINNLLQ VKMRKRYSVD 800
KSLSHPWLQE YQTWLDLREL EGKMGERYIT HESDDARWDQ FVSERHGTPA 850
EGDLGGACLP QDHEMQGLAE RISIL 875
Length:875
Mass (Da):96,497
Last modified:November 23, 2004 - v1
Checksum:i789FD9853EEDF6A1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC083592 mRNA. Translation: AAH83592.1.
RefSeqiNP_001013917.1. NM_001013895.1.
UniGeneiRn.15035.

Genome annotation databases

EnsembliENSRNOT00000022360; ENSRNOP00000022360; ENSRNOG00000016434.
GeneIDi292658.
KEGGirno:292658.
UCSCiRGD:1308054. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC083592 mRNA. Translation: AAH83592.1 .
RefSeqi NP_001013917.1. NM_001013895.1.
UniGenei Rn.15035.

3D structure databases

ProteinModelPortali Q5XIS9.
SMRi Q5XIS9. Positions 396-510.
ModBasei Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000022360.

PTM databases

PhosphoSitei Q5XIS9.

Proteomic databases

PaxDbi Q5XIS9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000022360 ; ENSRNOP00000022360 ; ENSRNOG00000016434 .
GeneIDi 292658.
KEGGi rno:292658.
UCSCi RGD:1308054. rat.

Organism-specific databases

CTDi 25865.
RGDi 1308054. Prkd2.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00750000117523.
HOGENOMi HOG000015135.
HOVERGENi HBG003564.
InParanoidi Q5XIS9.
KOi K06070.
OMAi LPQDHEM.
OrthoDBi EOG75B84N.
PhylomeDBi Q5XIS9.
TreeFami TF314320.

Miscellaneous databases

NextBioi 634460.
PROi Q5XIS9.

Gene expression databases

Genevestigatori Q5XIS9.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
InterProi IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR015727. Protein_Kinase_C_mu-related.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
PANTHERi PTHR22968. PTHR22968. 1 hit.
Pfami PF00130. C1_1. 2 hits.
PF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000552. PKC_mu_nu_D2. 1 hit.
PRINTSi PR00008. DAGPEDOMAIN.
SMARTi SM00109. C1. 2 hits.
SM00233. PH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiKPCD2_RAT
AccessioniPrimary (citable) accession number: Q5XIS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 23, 2004
Last modified: September 3, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi