Q5XIS9 (KPCD2_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified April 3, 2013. Version 90. History...
Names and origin
|Protein names||Recommended name:|
Serine/threonine-protein kinase D2
|Organism||Rattus norvegicus (Rat) [Reference proteome]|
|Taxonomic identifier||10116 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus|
|Sequence length||875 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of cell proliferation via MAPK1/3 (ERK1/2) signaling, oxidative stress-induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, signaling downstream of T-cell antigen receptor (TCR) and cytokine production, and plays a role in Golgi membrane trafficking, angiogenesis, secretory granule release and cell adhesion. May potentiate mitogenesis induced by the neuropeptide bombesin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression. In response to oxidative stress, is phosphorylated at Tyr-439 by ABL1, which leads to the activation of PRKD2 without increasing its catalytic activity, and mediates activation of NF-kappa-B. In response to the activation of the gastrin receptor CCKBR, is phosphorylated at Ser-244 by CSNK1D and CSNK1E, translocates to the nucleus, phosphorylates HDAC7, leading to nuclear export of HDAC7 and inhibition of HDAC7 transcriptional repression of NR4A1/NUR77. Upon TCR stimulation, is activated independently of ZAP70, translocates from the cytoplasm to the nucleus and is required for interleukin-2 (IL2) promoter up-regulation. During adaptive immune responses, is required in peripheral T-lymphocytes for the production of the effector cytokines IL2 and IFNG after TCR engagement and for optimal induction of antibody responses to antigens. In epithelial cells stimulated with lysophosphatidic acid (LPA), is activated through a PKC-dependent pathway and mediates LPA-stimulated interleukin-8 (IL8) secretion via a NF-kappa-B-dependent pathway. During TCR-induced T-cell activation, interacts with and is activated by the tyrosine kinase LCK, which results in the activation of the NFAT transcription factors. In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane and in polarized cells is involved in the transport of proteins from the TGN to the basolateral membrane. Plays an important role in endothelial cell proliferation and migration prior to angiogenesis, partly through modulation of the expression of KDR/VEGFR2 and FGFR1, two key growth factor receptors involved in angiogenesis. In secretory pathway, is required for the release of chromogranin-A (CHGA)-containing secretory granules from the TGN. Downstream of PRKCA, plays important roles in angiotensin-2-induced monocyte adhesion to endothelial cells By similarity.
ATP + a protein = ADP + a phosphoprotein.
Magnesium By similarity.
Activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-711 and phosphorylation of Ser-707 by PKC relieves auto-inhibition by the PH domain By similarity.
Interacts (via C-terminus) with LCK By similarity.
Cytoplasm By similarity. Cell membrane By similarity. Golgi apparatus › trans-Golgi network By similarity. Note: Translocation to the cell membrane is required for kinase activation. Accumulates in the nucleus upon CK1-mediated phosphorylation after activation of G-protein-coupled receptors. Nuclear accumulation is regulated by blocking nuclear export of active PRKD2 rather than by increasing import By similarity.
Phosphorylation of Ser-873 correlates with the activation status of the kinase. Ser-707 is probably phosphorylated by PKC. Phosphorylation at Ser-244 by CSNK1D and CSNK1E promotes nuclear localization and substrate targeting. Phosphorylation at Ser-244, Ser-707 and Ser-711 is required for nuclear localization. Phosphorylated at Tyr-438 by ABL1 in response to oxidative stress By similarity.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 875||875||Serine/threonine-protein kinase D2||PRO_0000260437|
|Domain||398 – 510||113||PH|
|Domain||552 – 808||257||Protein kinase|
|Zinc finger||138 – 188||51||Phorbol-ester/DAG-type 1|
|Zinc finger||265 – 315||51||Phorbol-ester/DAG-type 2|
|Nucleotide binding||558 – 566||9||ATP By similarity|
|Active site||675||1||Proton acceptor By similarity|
|Binding site||581||1||ATP By similarity|
Amino acid modifications
|Modified residue||200||1||Phosphoserine By similarity|
|Modified residue||203||1||Phosphoserine By similarity|
|Modified residue||206||1||Phosphoserine By similarity|
|Modified residue||211||1||Phosphoserine Ref.2|
|Modified residue||212||1||Phosphoserine By similarity|
|Modified residue||214||1||Phosphoserine Ref.2|
|Modified residue||244||1||Phosphoserine; by CSNK1D and CSNK1E By similarity|
|Modified residue||439||1||Phosphotyrosine; by ABL1 By similarity|
|Modified residue||519||1||Phosphoserine By similarity|
|Modified residue||707||1||Phosphoserine; by PKC By similarity|
|Modified residue||711||1||Phosphoserine; by autocatalysis By similarity|
|Modified residue||718||1||Phosphotyrosine By similarity|
|Modified residue||873||1||Phosphoserine; by autocatalysis By similarity|
|||"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."|
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
|||"Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."|
Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-211 AND SER-214, MASS SPECTROMETRY.
Tissue: Renal collecting duct.
|+||Additional computationally mapped references.|
|BC083592 mRNA. Translation: AAH83592.1.|
|RefSeq||NP_001013917.1. NM_001013895.1. |
3D structure databases
|HSSP||HSSP built from PDB template 2COA based on UniProtKB Q9BZL6. |
|SMR||Q5XIS9. Positions 396-510. |
Protein-protein interaction databases
Protocols and materials databases
Genome annotation databases
|Ensembl||ENSRNOT00000022360; ENSRNOP00000022360; ENSRNOG00000016434. |
|UCSC||RGD:1308054. rat. |
|RGD||1308054. Prkd2. |
Gene expression databases
|GermOnline||ENSRNOG00000016434. Rattus norvegicus. |
Family and domain databases
|Gene3D||18.104.22.168. 1 hit. |
|InterPro||IPR020454. DAG/PE-bd. |
|PANTHER||PTHR22968. PTHR22968. 1 hit. |
|Pfam||PF00130. C1_1. 2 hits. |
PF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
|PIRSF||PIRSF000552. PKC_mu_nu_D2. 1 hit. |
|PRINTS||PR00008. DAGPEDOMAIN. |
|SMART||SM00109. C1. 2 hits. |
SM00233. PH. 1 hit.
SM00220. S_TKc. 1 hit.
|SUPFAM||SSF56112. Kinase_like. 1 hit. |
|PROSITE||PS50003. PH_DOMAIN. 1 hit. |
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
|Accession||Primary (citable) accession number: Q5XIS9|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
Index of protein domains and families