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Protein

Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1

Gene

Pomgnt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Participates in O-mannosyl glycosylation by catalyzing the addition of N-acetylglucosamine to O-linked mannose on glycoproteins. Catalyzes the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins, providing the necessary basis for the addition of further carbohydrate moieties. Is specific for alpha linked terminal mannose.By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + O-alpha-D-mannosylprotein = UDP + N-acetyl-beta-D-glucosaminyl-(1->2)-O-alpha-D-mannosylprotein.By similarity

Cofactori

Mn2+By similarityNote: The manganese ion interacts primarily with the substrate UDP-N-acetylglucosamine.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei129CarbohydrateBy similarity1
Binding sitei179CarbohydrateBy similarity1
Binding sitei207CarbohydrateBy similarity1
Binding sitei338UDP-GlcNAcBy similarity1
Binding sitei371UDP-GlcNAcBy similarity1
Metal bindingi395ManganeseBy similarity1
Metal bindingi500ManganeseBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi307 – 311UDP-GlcNAcBy similarity5
Nucleotide bindingi394 – 395UDP-GlcNAcBy similarity2
Nucleotide bindingi506 – 507UDP-GlcNAcBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-5173105 O-linked glycosylation
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT13 Glycosyltransferase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 (EC:2.4.1.-By similarity)
Short name:
POMGnT1
Gene namesi
Name:Pomgnt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1359396 Pomgnt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 37CytoplasmicSequence analysisAdd BLAST37
Transmembranei38 – 58Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini59 – 660LumenalSequence analysisAdd BLAST602

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001913931 – 660Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1Add BLAST660

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphoserineBy similarity1
Disulfide bondi254 ↔ 281By similarity
Disulfide bondi269 ↔ 279By similarity
Disulfide bondi421 ↔ 490By similarity
Disulfide bondi562 ↔ 596By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ5XIN7
PRIDEiQ5XIN7

Expressioni

Gene expression databases

BgeeiENSRNOG00000023455
GenevisibleiQ5XIN7 RN

Interactioni

Subunit structurei

Interacts with DAG1 (via O-linked mannose moiety). Interacts (via transmembrane domain) with FKTN; the interaction is direct and is required for normal location in Golgi membranes.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000035825

Structurei

3D structure databases

ProteinModelPortaliQ5XIN7
SMRiQ5XIN7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni92 – 288Carbohydrate-binding stem domainBy similarityAdd BLAST197
Regioni300 – 646CatalyticBy similarityAdd BLAST347
Regioni473 – 481Interaction with O-glycosylated substrate glycoproteinBy similarity9
Regioni506 – 512Interaction with O-glycosylated substrate glycoproteinBy similarity7
Regioni600 – 605Interaction with O-glycosylated substrate glycoproteinBy similarity6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi263 – 267Poly-Arg5

Domaini

The stem domain mediates specific interaction with beta-linked N-acetylglucosamine moieties of O-glycosylated proteins. It also interacts with its product, N-acetyl-beta-D-glucosaminyl-(1->2)-O-alpha-D-mannosylprotein.By similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 13 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFXX Eukaryota
ENOG410YMAS LUCA
GeneTreeiENSGT00530000063632
HOGENOMiHOG000231121
HOVERGENiHBG055279
InParanoidiQ5XIN7
KOiK09666
OMAiPYSVKKP
OrthoDBiEOG091G00JT
PhylomeDBiQ5XIN7
TreeFamiTF320555

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR004139 Glyco_trans_13
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR10468 PTHR10468, 1 hit
PfamiView protein in Pfam
PF03071 GNT-I, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

Q5XIN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDWKPSPLI KPFGARKKRS WYLTWKYKLT NQRALRRFCQ TGAVLFLLVT
60 70 80 90 100
VIVNIKLILD TRRAISEANE DPEPEQDYDE ALGRLESPRH RGSSPRRVLD
110 120 130 140 150
VEVYSSRSKV YVAVDGTTVL EDEAREQGRG IHVIVLNQAT GHVMAKRVFD
160 170 180 190 200
TYSPHEDEAM VLFLNMVAPG RVLICTVKDE GSFHLKDTAK ALLRSLGSQA
210 220 230 240 250
GPALGWRDTW AFVGRKGGPV LGEKHSKSPA LSSWGDPVLL KTDVPLSSAE
260 270 280 290 300
EAECHWADTE LNRRRRRFCS KVEGYGSVCS CKDPTPIEFS PDPLPDNKVL
310 320 330 340 350
NVPVAVIAGN RPNYLYRMLR SLLSAQGVSP QMITVFIDGY YEEPMDVVAL
360 370 380 390 400
FGLRGIQHTP ISIKNARVSQ HYKASLTATF NLFPEAKFAV VLEEDLDIAV
410 420 430 440 450
DFFSFLSQSI HLLEEDDSLY CISAWNDQGY EHTAEDPALL YRVETMPGLG
460 470 480 490 500
WVLRKSLYKE ELEPKWPTPE KLWDWDMWMR MPEQRRGREC IIPDVSRSYH
510 520 530 540 550
FGIVGLNMNG YFHEAYFKKH KFNTVPGVQL RNVDSLKKEA YEVEIHRLLS
560 570 580 590 600
EAEVLDHSKD PCEDSFLPDT EGHTYVAFIR MEKDDDFTTW TQLAKCLHIW
610 620 630 640 650
DLDVRGNHRG LWRLFRKKNH FLVVGVPASP YSVKKPPSVT PIFLEPPPKE
660
EGAPGAAEQT
Length:660
Mass (Da):75,148
Last modified:November 23, 2004 - v1
Checksum:iEA6B2E3A9034CB9E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083641 mRNA Translation: AAH83641.1
RefSeqiNP_001007748.1, NM_001007747.1
UniGeneiRn.162088

Genome annotation databases

EnsembliENSRNOT00000035312; ENSRNOP00000035825; ENSRNOG00000023455
GeneIDi362567
KEGGirno:362567
UCSCiRGD:1359396 rat

Similar proteinsi

Entry informationi

Entry nameiPMGT1_RAT
AccessioniPrimary (citable) accession number: Q5XIN7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: November 23, 2004
Last modified: May 23, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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