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Protein

Protein Mdm4

Gene

Mdm4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri300 – 32930RanBP2-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri437 – 47842RING-type; degeneratePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-6804756. Regulation of TP53 Activity through Phosphorylation.
R-RNO-6804757. Regulation of TP53 Degradation.
R-RNO-6804760. Regulation of TP53 Activity through Methylation.
R-RNO-69541. Stabilization of p53.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Mdm4
Alternative name(s):
Double minute 4 protein
Mdm2-like p53-binding protein
Protein Mdmx
p53-binding protein Mdm4
Gene namesi
Name:Mdm4
Synonyms:Mdmx
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi1309306. Mdm4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Protein Mdm4PRO_0000331516Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei180 – 1801PhosphoserineCombined sources
Modified residuei342 – 3421Phosphoserine; by CHEK2By similarity
Modified residuei368 – 3681Phosphoserine; by CHEK1 and CHEK2By similarity

Post-translational modificationi

Phosphorylated. Phosphorylation at Ser-368 promotes interaction with YWHAG and subsequent ubiquitination and degradation. Phosphorylation at Ser-342 also induces ubiquitination and degradation but to a lower extent (By similarity).By similarity
Ubiquitinated and degraded by MDM2. Deubiquitination by USP2 on the other hand stabilizes the MDM4 protein (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5XIN1.
PRIDEiQ5XIN1.

PTM databases

iPTMnetiQ5XIN1.
PhosphoSiteiQ5XIN1.

Expressioni

Gene expression databases

BgeeiENSRNOG00000009696.
GenevisibleiQ5XIN1. RN.

Interactioni

Subunit structurei

Interacts with MDM2, TP53, TP73 and USP2. Found in a trimeric complex with USP2, MDM2 and MDM4. Interacts (phosphorylated) with YWHAG; negatively regulates MDM4 activity toward TP53 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012984.

Structurei

3D structure databases

ProteinModelPortaliQ5XIN1.
SMRiQ5XIN1. Positions 27-108, 301-344, 428-490.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 10276SWIBAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni393 – 49098Necessary for interaction with USP2By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi442 – 4454Nuclear localization signalSequence analysis

Sequence similaritiesi

Belongs to the MDM2/MDM4 family.Curated
Contains 1 RanBP2-type zinc finger.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 1 SWIB domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri300 – 32930RanBP2-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri437 – 47842RING-type; degeneratePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IE9V. Eukaryota.
ENOG410XSWN. LUCA.
GeneTreeiENSGT00530000063539.
HOGENOMiHOG000293341.
HOVERGENiHBG013472.
InParanoidiQ5XIN1.
KOiK10127.
OMAiTENMEDC.
OrthoDBiEOG091G0FYK.
PhylomeDBiQ5XIN1.
TreeFamiTF105306.

Family and domain databases

Gene3Di1.10.245.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR015458. MDM4.
IPR016495. p53_neg-reg_MDM_2/4.
IPR003121. SWIB_MDM2_domain.
IPR001876. Znf_RanBP2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF02201. SWIB. 1 hit.
PF00641. zf-RanBP. 1 hit.
[Graphical view]
PIRSFiPIRSF500699. MDM4. 1 hit.
PIRSF006748. p53_MDM_2/4. 1 hit.
SUPFAMiSSF47592. SSF47592. 1 hit.
SSF90209. SSF90209. 1 hit.
PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5XIN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSHSTSAQC SASDSACRIS SEQINQQVRP KLQLLKILQA AGAQGEVFTM
60 70 80 90 100
KEVMHYLGQY IMVKQLYDQQ EQHLVYCGGD LLGDLLGCQS FSVKDPSPLY
110 120 130 140 150
DMLRKNLVTS ASVNTDAAQT LALAQDHTMD IPSQDRLKHG ATECSNPRKR
160 170 180 190 200
TEEDDTHTLP TSRRKCRDSR ADEDLIEHLS QDETSRLDLD FEEWDVAGLP
210 220 230 240 250
WWFLGNLRNN YIPRSNGSTD LQTNQDIGTA IVSDTTDDLW FLNETVSEQL
260 270 280 290 300
GVGVKVEAAN CEQPSEVGRT RDKKMVEGGK DDDLEDSKSL SDDTDVEVTS
310 320 330 340 350
EDEWQCTECK KFNSPSKRYC FRCWALRKDW YSDCSKLTHS LSTSNITAIP
360 370 380 390 400
EKKDNEGIDV PDCRRTISAP VVRPKDGYLK EEKPRFDPCN SVEFLDLAHG
410 420 430 440 450
SESQEIISST REQTDIFSEQ KTETESMEDF QNVLKPCSLC EKRPRDGNII
460 470 480 490
HGKTSHLTTC FHCARRLKKS GASCPACKKE IQLVIKVFIA
Length:490
Mass (Da):55,221
Last modified:November 23, 2004 - v1
Checksum:i7286E5E024E8AAE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083647 mRNA. Translation: AAH83647.1.
RefSeqiNP_001012026.1. NM_001012026.1.
UniGeneiRn.146603.
Rn.90967.

Genome annotation databases

EnsembliENSRNOT00000012984; ENSRNOP00000012984; ENSRNOG00000009696.
GeneIDi304798.
KEGGirno:304798.
UCSCiRGD:1309306. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083647 mRNA. Translation: AAH83647.1.
RefSeqiNP_001012026.1. NM_001012026.1.
UniGeneiRn.146603.
Rn.90967.

3D structure databases

ProteinModelPortaliQ5XIN1.
SMRiQ5XIN1. Positions 27-108, 301-344, 428-490.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012984.

PTM databases

iPTMnetiQ5XIN1.
PhosphoSiteiQ5XIN1.

Proteomic databases

PaxDbiQ5XIN1.
PRIDEiQ5XIN1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012984; ENSRNOP00000012984; ENSRNOG00000009696.
GeneIDi304798.
KEGGirno:304798.
UCSCiRGD:1309306. rat.

Organism-specific databases

CTDi4194.
RGDi1309306. Mdm4.

Phylogenomic databases

eggNOGiENOG410IE9V. Eukaryota.
ENOG410XSWN. LUCA.
GeneTreeiENSGT00530000063539.
HOGENOMiHOG000293341.
HOVERGENiHBG013472.
InParanoidiQ5XIN1.
KOiK10127.
OMAiTENMEDC.
OrthoDBiEOG091G0FYK.
PhylomeDBiQ5XIN1.
TreeFamiTF105306.

Enzyme and pathway databases

ReactomeiR-RNO-6804756. Regulation of TP53 Activity through Phosphorylation.
R-RNO-6804757. Regulation of TP53 Degradation.
R-RNO-6804760. Regulation of TP53 Activity through Methylation.
R-RNO-69541. Stabilization of p53.

Miscellaneous databases

PROiQ5XIN1.

Gene expression databases

BgeeiENSRNOG00000009696.
GenevisibleiQ5XIN1. RN.

Family and domain databases

Gene3Di1.10.245.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR015458. MDM4.
IPR016495. p53_neg-reg_MDM_2/4.
IPR003121. SWIB_MDM2_domain.
IPR001876. Znf_RanBP2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF02201. SWIB. 1 hit.
PF00641. zf-RanBP. 1 hit.
[Graphical view]
PIRSFiPIRSF500699. MDM4. 1 hit.
PIRSF006748. p53_MDM_2/4. 1 hit.
SUPFAMiSSF47592. SSF47592. 1 hit.
SSF90209. SSF90209. 1 hit.
PROSITEiPS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDM4_RAT
AccessioniPrimary (citable) accession number: Q5XIN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: November 23, 2004
Last modified: September 7, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.