Q5XIL2 (P4K2B_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 4-kinase type 2-beta
EC=2.7.1.67

Alternative name(s):
Phosphatidylinositol 4-kinase type II-beta

Gene names

Name:

Pi4k2b

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking By similarity.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.
Enzyme regulation	Recruited and activated by membrane association. Binding to the microsomal membrane has been shown to enhance its activity By similarity.
Subcellular location	Cytoplasm By similarity. Membrane By similarity. Note: Mostly cytoplasmic but also found associated with the plasma membrane, the Golgi and endosomes. Compared to PI4K2A, a larger fraction of PI4K2B is cytosolic due to a smaller extent of palmitoylation. Translocates to membranes where it is recruited by PDGF stimulation by a Rac-GTP-dependent mechanism By similarity.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily. Contains 1 PI3K/PI4K domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-phosphatidylinositol 4-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	Phosphatidylinositol 4-kinase type 2-beta		PRO_0000285166
Regions
	Domain	125 – 432	308	PI3K/PI4K
Amino acid modifications
	Modified residue	45	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5XIL2 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: BCC6EECB4594F652	FASTA	477	54,464
	10 20 30 40 50 60 MPEPPRDIMA EACEATRPSE DEDEEREPLL PRVAWAQPRR GAPGSAVRLQ ADQGAAVLRE 70 80 90 100 110 120 PATEEPPVVS EDRSISASLS TELDRTRNAV SETNTFLEDP EFADVVLKAE QAIEIGVFPE 130 140 150 160 170 180 RISQGSSGSY FVKDSKRTII GVFKPKSEEP YGQLNPKWTK YVHKVCCPCC FGRGCLLPNQ 190 200 210 220 230 240 GYLSEAGAYL VDTKLQLGIV PKTKVVWLVS ETFNYSAIDR AKSRGKKYAL EKVPKVGRKF 250 260 270 280 290 300 HRIGLPPKIG SFQLFVKDYK EAEYWLRRFE AEPLPENIRK QFQSQFERLV ILDYIIRNTD 310 320 330 340 350 360 RGNDNWLVKY DEMKYAKKIE SEESNWIDDK QLLIRIAAID NGLAFPFKHP DEWRAYPFHW 370 380 390 400 410 420 AWLPQAKVPF SEETRNLILP FISDMNFVQD LCEDLYELFK TDKGFDRAAF ESQMSVMRGQ 430 440 450 460 470 ILNLTQALRD GKSPMQLAQM PCVIVECSKS GGQGRVVHLG SSFTQTVHCR KPFFSSW

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC083668 mRNA. Translation: AAH83668.1.
IPI	IPI00364583.
RefSeq	NP_001005883.1. NM_001005883.1.
UniGene	Rn.21189.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5XIL2.
Proteomic databases
PRIDE	Q5XIL2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000005349; ENSRNOP00000005349; ENSRNOG00000003924.
GeneID	305419.
KEGG	rno:305419.
UCSC	NM_001005883. rat.
Organism-specific databases
CTD	55300.
RGD	1359515. Pi4k2b.
Phylogenomic databases
eggNOG	roNOG05931.
GeneTree	ENSGT00390000010434.
HOVERGEN	HBG059542.
InParanoid	Q5XIL2.
OMA	AEQAIEF.
OrthoDB	EOG4XSKPT.
PhylomeDB	Q5XIL2.
Gene expression databases
ArrayExpress	Q5XIL2.
Genevestigator	Q5XIL2.
Family and domain databases
InterPro	IPR000403. PI3/4_kinase_cat. [Graphical view]
KO	K13711.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. [Graphical view]
PROSITE	PS00915. PI3_4_KINASE_1. False negative. PS00916. PI3_4_KINASE_2. False negative. PS50290. PI3_4_KINASE_3. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	654495.

Entry information

Entry name

P4K2B_RAT

Accession

Primary (citable) accession number: Q5XIL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	November 23, 2004
Last modified:	November 16, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program