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Protein

Phosphatidylinositol 4-kinase type 2-beta

Gene

Pi4k2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking (By similarity).By similarity

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.

Enzyme regulationi

Recruited and activated by membrane association. Binding to the microsomal membrane has been shown to enhance its activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei144 – 1441ATPBy similarity
Binding sitei340 – 3401ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi123 – 1297ATPBy similarity
Nucleotide bindingi253 – 2564ATPBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-1483248. Synthesis of PIPs at the ER membrane.
R-RNO-1660499. Synthesis of PIPs at the plasma membrane.
R-RNO-1660514. Synthesis of PIPs at the Golgi membrane.
R-RNO-1660516. Synthesis of PIPs at the early endosome membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4-kinase type 2-beta (EC:2.7.1.67)
Alternative name(s):
Phosphatidylinositol 4-kinase type II-beta
Gene namesi
Name:Pi4k2b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi1359515. Pi4k2b.

Subcellular locationi

  • Cytoplasm By similarity
  • Membrane By similarity

  • Note: Mostly cytoplasmic but also found associated with the plasma membrane, the Golgi and endosomes. Compared to PI4K2A, a larger fraction of PI4K2B is cytosolic due to a smaller extent of palmitoylation. Translocates to membranes where it is recruited by PDGF stimulation by a Rac-GTP-dependent mechanism (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Phosphatidylinositol 4-kinase type 2-betaPRO_0000285166Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei45 – 451PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5XIL2.
PRIDEiQ5XIL2.

Expressioni

Gene expression databases

GenevisibleiQ5XIL2. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005349.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini125 – 432308PI3K/PI4KAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni149 – 1513Important for substrate bindingBy similarity
Regioni157 – 17014Important for interaction with membranesBy similarityAdd
BLAST
Regioni260 – 2689Important for interaction with membranesBy similarity
Regioni353 – 36210Important for interaction with membranesBy similarity

Sequence similaritiesi

Contains 1 PI3K/PI4K domain.Curated

Phylogenomic databases

eggNOGiKOG2381. Eukaryota.
ENOG410XP06. LUCA.
GeneTreeiENSGT00390000010434.
HOGENOMiHOG000294076.
HOVERGENiHBG059542.
InParanoidiQ5XIL2.
KOiK13711.
OMAiCEDLHEL.
OrthoDBiEOG7MKW5Z.
PhylomeDBiQ5XIL2.
TreeFamiTF314740.

Family and domain databases

InterProiIPR000403. PI3/4_kinase_cat_dom.
[Graphical view]
PfamiPF00454. PI3_PI4_kinase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5XIL2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEPPRDIMA EACEATRPSE DEDEEREPLL PRVAWAQPRR GAPGSAVRLQ
60 70 80 90 100
ADQGAAVLRE PATEEPPVVS EDRSISASLS TELDRTRNAV SETNTFLEDP
110 120 130 140 150
EFADVVLKAE QAIEIGVFPE RISQGSSGSY FVKDSKRTII GVFKPKSEEP
160 170 180 190 200
YGQLNPKWTK YVHKVCCPCC FGRGCLLPNQ GYLSEAGAYL VDTKLQLGIV
210 220 230 240 250
PKTKVVWLVS ETFNYSAIDR AKSRGKKYAL EKVPKVGRKF HRIGLPPKIG
260 270 280 290 300
SFQLFVKDYK EAEYWLRRFE AEPLPENIRK QFQSQFERLV ILDYIIRNTD
310 320 330 340 350
RGNDNWLVKY DEMKYAKKIE SEESNWIDDK QLLIRIAAID NGLAFPFKHP
360 370 380 390 400
DEWRAYPFHW AWLPQAKVPF SEETRNLILP FISDMNFVQD LCEDLYELFK
410 420 430 440 450
TDKGFDRAAF ESQMSVMRGQ ILNLTQALRD GKSPMQLAQM PCVIVECSKS
460 470
GGQGRVVHLG SSFTQTVHCR KPFFSSW
Length:477
Mass (Da):54,464
Last modified:November 23, 2004 - v1
Checksum:iBCC6EECB4594F652
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083668 mRNA. Translation: AAH83668.1.
RefSeqiNP_001005883.1. NM_001005883.1.
UniGeneiRn.21189.

Genome annotation databases

EnsembliENSRNOT00000005349; ENSRNOP00000005349; ENSRNOG00000003924.
GeneIDi305419.
KEGGirno:305419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083668 mRNA. Translation: AAH83668.1.
RefSeqiNP_001005883.1. NM_001005883.1.
UniGeneiRn.21189.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005349.

Proteomic databases

PaxDbiQ5XIL2.
PRIDEiQ5XIL2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000005349; ENSRNOP00000005349; ENSRNOG00000003924.
GeneIDi305419.
KEGGirno:305419.

Organism-specific databases

CTDi55300.
RGDi1359515. Pi4k2b.

Phylogenomic databases

eggNOGiKOG2381. Eukaryota.
ENOG410XP06. LUCA.
GeneTreeiENSGT00390000010434.
HOGENOMiHOG000294076.
HOVERGENiHBG059542.
InParanoidiQ5XIL2.
KOiK13711.
OMAiCEDLHEL.
OrthoDBiEOG7MKW5Z.
PhylomeDBiQ5XIL2.
TreeFamiTF314740.

Enzyme and pathway databases

ReactomeiR-RNO-1483248. Synthesis of PIPs at the ER membrane.
R-RNO-1660499. Synthesis of PIPs at the plasma membrane.
R-RNO-1660514. Synthesis of PIPs at the Golgi membrane.
R-RNO-1660516. Synthesis of PIPs at the early endosome membrane.

Miscellaneous databases

NextBioi654495.
PROiQ5XIL2.

Gene expression databases

GenevisibleiQ5XIL2. RN.

Family and domain databases

InterProiIPR000403. PI3/4_kinase_cat_dom.
[Graphical view]
PfamiPF00454. PI3_PI4_kinase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiP4K2B_RAT
AccessioniPrimary (citable) accession number: Q5XIL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: November 23, 2004
Last modified: November 11, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.