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Q5XIH4

- LIAS_RAT

UniProt

Q5XIH4 - LIAS_RAT

Protein

Lipoyl synthase, mitochondrial

Gene

Lias

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi105 – 1051Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi110 – 1101Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi116 – 1161Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi136 – 1361Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi140 – 1401Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi143 – 1431Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. inflammatory response Source: Ensembl
    2. neural tube closure Source: Ensembl
    3. protein lipoylation Source: UniProtKB-HAMAP
    4. response to lipopolysaccharide Source: Ensembl
    5. response to oxidative stress Source: Ensembl

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    Name:Lias
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 14

    Organism-specific databases

    RGDi1307270. Lias.

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2626MitochondrionUniRule annotationAdd
    BLAST
    Chaini27 – 373347Lipoyl synthase, mitochondrialPRO_0000332311Add
    BLAST

    Proteomic databases

    PaxDbiQ5XIH4.
    PRIDEiQ5XIH4.

    Expressioni

    Gene expression databases

    GenevestigatoriQ5XIH4.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000003779.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5XIH4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0320.
    GeneTreeiENSGT00390000006234.
    HOGENOMiHOG000235998.
    HOVERGENiHBG023328.
    InParanoidiQ5XIH4.
    KOiK03644.
    OMAiPEEPYNT.
    OrthoDBiEOG7P2XS7.
    PhylomeDBiQ5XIH4.
    TreeFamiTF300817.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5XIH4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALRCWDAAR SLGSRIFGRY ACSVRALSSL PDEKKKFLHN GPDLQDFVSG    50
    DLADKSTWDD YKGNLKRQKG ERLRLPPWLK TKIPMGKNYN KLKNTLRNLN 100
    LHTVCEEARC PNIGECWGGG EYATATATIM LMGDTCTRGC RFCSVKTARN 150
    PPPLDPSEPD NTARAIAEWG LDYVVLTSVD RDDVVDGGAE HIAKTVSCLK 200
    ERNPKILVEC LTPDFRGDLR AVEKVALSGL DVYAHNVETV PELQRKVRDP 250
    RANFDQSLRV LKHAKEVQPD VVSKTSIMLG LGETDEQVYA TMKALRAADV 300
    DCLTLGQYMQ PTKRHLKVEE YVTPEKFKYW EEVGNELGFH YTASGPLVRS 350
    SYKAGEFFLK NLVAKRKTKV SKV 373
    Length:373
    Mass (Da):41,874
    Last modified:November 23, 2004 - v1
    Checksum:iF2108A035F9C7B14
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC083708 mRNA. Translation: AAH83708.1.
    RefSeqiNP_001012037.1. NM_001012037.1.
    UniGeneiRn.25547.

    Genome annotation databases

    EnsembliENSRNOT00000003779; ENSRNOP00000003779; ENSRNOG00000002759.
    GeneIDi305348.
    KEGGirno:305348.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC083708 mRNA. Translation: AAH83708.1 .
    RefSeqi NP_001012037.1. NM_001012037.1.
    UniGenei Rn.25547.

    3D structure databases

    ProteinModelPortali Q5XIH4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000003779.

    Proteomic databases

    PaxDbi Q5XIH4.
    PRIDEi Q5XIH4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000003779 ; ENSRNOP00000003779 ; ENSRNOG00000002759 .
    GeneIDi 305348.
    KEGGi rno:305348.

    Organism-specific databases

    CTDi 11019.
    RGDi 1307270. Lias.

    Phylogenomic databases

    eggNOGi COG0320.
    GeneTreei ENSGT00390000006234.
    HOGENOMi HOG000235998.
    HOVERGENi HBG023328.
    InParanoidi Q5XIH4.
    KOi K03644.
    OMAi PEEPYNT.
    OrthoDBi EOG7P2XS7.
    PhylomeDBi Q5XIH4.
    TreeFami TF300817.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Miscellaneous databases

    NextBioi 654409.
    PROi Q5XIH4.

    Gene expression databases

    Genevestigatori Q5XIH4.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Heart.

    Entry informationi

    Entry nameiLIAS_RAT
    AccessioniPrimary (citable) accession number: Q5XIH4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3