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Protein

E3 ubiquitin-protein ligase MARCH3

Gene

March3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase which may be involved in endosomal trafficking. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri63 – 123RING-CH-typePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processEndocytosis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase MARCH3 (EC:2.3.2.27)
Alternative name(s):
Membrane-associated RING finger protein 3
Membrane-associated RING-CH protein III
Short name:
MARCH-III
RING-type E3 ubiquitin transferase MARCH3Curated
Gene namesi
Name:March3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi1359308. March3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei145 – 165HelicalSequence analysisAdd BLAST21
Transmembranei182 – 202HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000559291 – 253E3 ubiquitin-protein ligase MARCH3Add BLAST253

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei237PhosphoserineBy similarity1
Modified residuei243PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5XIE5.

PTM databases

iPTMnetiQ5XIE5.
PhosphoSitePlusiQ5XIE5.

Expressioni

Tissue specificityi

Expressed predominantly in lung, colon and spleen. Present in liver (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000023013.
GenevisibleiQ5XIE5. RN.

Interactioni

Subunit structurei

Interacts with MARCH2 and STX6.1 Publication

Protein-protein interaction databases

BioGridi265017. 2 interactors.
STRINGi10116.ENSRNOP00000030051.

Structurei

3D structure databases

ProteinModelPortaliQ5XIE5.
SMRiQ5XIE5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-CH-type zinc finger domain is required for E3 ligase activity.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri63 – 123RING-CH-typePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1609. Eukaryota.
COG5183. LUCA.
GeneTreeiENSGT00730000110355.
HOGENOMiHOG000293394.
HOVERGENiHBG052411.
InParanoidiQ5XIE5.
KOiK10658.
OMAiCELCHFK.
OrthoDBiEOG091G14LW.
PhylomeDBiQ5XIE5.
TreeFamiTF319557.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiView protein in InterPro
IPR033275. MARCH-like.
IPR011016. Znf_RING-CH.
IPR013083. Znf_RING/FYVE/PHD.
PANTHERiPTHR23012. PTHR23012. 1 hit.
PfamiView protein in Pfam
PF12906. RINGv. 1 hit.
SMARTiView protein in SMART
SM00744. RINGv. 1 hit.
PROSITEiView protein in PROSITE
PS51292. ZF_RING_CH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5XIE5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSRCSHLP EVLPDCTSSA APVVKTVEDC GSLVNGQPQY VMQVSAKDGQ
60 70 80 90 100
LLSTVVRTLA TQSPFNDRPM CRICHEGSSQ EDLLSPCECT GTLGTIHRSC
110 120 130 140 150
LEHWLSSSNT SYCELCHFRF AVERKPRPLV EWLRNPGPQH EKRTLFGDMV
160 170 180 190 200
CFLFITPLAT ISGWLCLRGA VDHLHFSSRL EAVGLIALTV ALFTIYLFWT
210 220 230 240 250
LVSFRYHCRL YNEWRRTNQR VILLIPKSVN IPSNQQSLLG LHSVKRNSKE

TIV
Length:253
Mass (Da):28,563
Last modified:November 23, 2004 - v1
Checksum:iC11A4D05CEBC9CE4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB048840 mRNA. Translation: BAE47142.1.
BC083738 mRNA. Translation: AAH83738.1.
RefSeqiNP_001007760.1. NM_001007759.1.
XP_006254811.1. XM_006254749.3.
XP_008770381.1. XM_008772159.2.
XP_017456494.1. XM_017601005.1.
XP_017456495.1. XM_017601006.1.
UniGeneiRn.203312.

Genome annotation databases

EnsembliENSRNOT00000039177; ENSRNOP00000030051; ENSRNOG00000023013.
ENSRNOT00000081020; ENSRNOP00000071176; ENSRNOG00000023013.
GeneIDi364878.
KEGGirno:364878.
UCSCiRGD:1359308. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMARH3_RAT
AccessioniPrimary (citable) accession number: Q5XIE5
Secondary accession number(s): Q33E96
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: November 23, 2004
Last modified: June 7, 2017
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways