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Protein

Enoyl-CoA delta isomerase 2, mitochondrial

Gene

Eci2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates.1 Publication

Catalytic activityi

(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei90 – 901Acyl-CoABy similarity
Binding sitei109 – 1091Acyl-CoABy similarity
Sitei277 – 2771Important for catalytic activityBy similarity

GO - Molecular functioni

GO - Biological processi

  • fatty acid beta-oxidation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

SABIO-RKQ5XIC0.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-CoA delta isomerase 2, mitochondrial (EC:5.3.3.8)
Alternative name(s):
Delta(3),delta(2)-enoyl-CoA isomerase
Short name:
D3,D2-enoyl-CoA isomerase
Dodecenoyl-CoA isomerase
Peroxisomal 3,2-trans-enoyl-CoA isomerase
Short name:
pECI
Gene namesi
Name:Eci2
Synonyms:Peci
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi1359427. Eci2.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: Ensembl
  • mitochondrion Source: RGD
  • nucleoplasm Source: Ensembl
  • peroxisomal matrix Source: UniProtKB-SubCell
  • peroxisome Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3636MitochondrionSequence analysisAdd
BLAST
Chaini37 – 391355Enoyl-CoA delta isomerase 2, mitochondrialPRO_0000381943Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei49 – 491N6-acetyllysine; alternateBy similarity
Modified residuei49 – 491N6-succinyllysine; alternateBy similarity
Modified residuei53 – 531N6-succinyllysineBy similarity
Modified residuei60 – 601N6-acetyllysine; alternateBy similarity
Modified residuei60 – 601N6-succinyllysine; alternateBy similarity
Modified residuei68 – 681N6-succinyllysineBy similarity
Modified residuei79 – 791N6-succinyllysineBy similarity
Modified residuei88 – 881N6-succinyllysineBy similarity
Modified residuei90 – 901N6-acetyllysine; alternateBy similarity
Modified residuei90 – 901N6-succinyllysine; alternateBy similarity
Modified residuei99 – 991PhosphoserineBy similarity
Modified residuei127 – 1271N6-succinyllysineBy similarity
Modified residuei159 – 1591N6-succinyllysineBy similarity
Modified residuei286 – 2861N6-succinyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5XIC0.
PRIDEiQ5XIC0.

PTM databases

iPTMnetiQ5XIC0.
PhosphoSiteiQ5XIC0.

Expressioni

Tissue specificityi

Liver (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiQ5XIC0. baseline and differential.
GenevisibleiQ5XIC0. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022022.

Structurei

3D structure databases

ProteinModelPortaliQ5XIC0.
SMRiQ5XIC0. Positions 29-131, 140-387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 12286ACBPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni64 – 685Acyl-CoA bindingBy similarity
Regioni149 – 319171ECH-likeAdd
BLAST
Regioni196 – 2005Substrate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi389 – 3913Microbody targeting signalSequence analysis

Sequence similaritiesi

In the C-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.Curated
Contains 1 ACB (acyl-CoA-binding) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0016. Eukaryota.
KOG0817. Eukaryota.
COG1024. LUCA.
COG4281. LUCA.
GeneTreeiENSGT00760000119100.
InParanoidiQ5XIC0.
KOiK13239.
OMAiKETARQN.
OrthoDBiEOG72RN06.
PhylomeDBiQ5XIC0.
TreeFamiTF313375.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
3.90.226.10. 1 hit.
InterProiIPR022408. Acyl-CoA-binding_prot_CS.
IPR000582. Acyl-CoA-binding_protein.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
[Graphical view]
PfamiPF00887. ACBP. 1 hit.
PF00378. ECH_1. 1 hit.
[Graphical view]
PRINTSiPR00689. ACOABINDINGP.
SUPFAMiSSF47027. SSF47027. 1 hit.
SSF52096. SSF52096. 1 hit.
PROSITEiPS00880. ACB_1. 1 hit.
PS51228. ACB_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms may exist.

Isoform 1 (identifier: Q5XIC0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAVTWSRAR CWCPSLLQVL RLPVTKLHLG RPAMRATQQD FENAMNQVKL
60 70 80 90 100
LKKDPGNEVK LRLYALYKQA TEGPCTMPKP GVFDFVNKAK WDAWNALGSL
110 120 130 140 150
PKETARQNYV DLVSSLSSSS EASSQGKGGA DGKAQESKGI LVTSEGGITK
160 170 180 190 200
ITFNRPSKKN AITFQMYQDI ILALKNASTD DTVITVFTGA GDYYSSGNDL
210 220 230 240 250
TNFTSASGGM EEAANKGAIV LREFVNTFID FPKPLVAVVN GPAVGISVTL
260 270 280 290 300
LGLFDAVYAS DRATFHTPFS HLGQSPEACS SYTFPKMMGS AKAAEMLLFG
310 320 330 340 350
KKLTAREAWA QGLVTEVFPE STFETEVWTR LKTYAKLPPN SMRISKELIR
360 370 380 390
KNEKEKLHAV NEEECTTLRA RWLSEECINA IMSFVTRKPK L
Length:391
Mass (Da):43,021
Last modified:November 23, 2004 - v1
Checksum:iA8BFC0E900967C32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083764 mRNA. Translation: AAH83764.1.
RefSeqiNP_001006967.1. NM_001006966.1. [Q5XIC0-1]
UniGeneiRn.108029.

Genome annotation databases

EnsembliENSRNOT00000022022; ENSRNOP00000022022; ENSRNOG00000029549. [Q5XIC0-1]
GeneIDi291075.
KEGGirno:291075.
UCSCiRGD:1359427. rat. [Q5XIC0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083764 mRNA. Translation: AAH83764.1.
RefSeqiNP_001006967.1. NM_001006966.1. [Q5XIC0-1]
UniGeneiRn.108029.

3D structure databases

ProteinModelPortaliQ5XIC0.
SMRiQ5XIC0. Positions 29-131, 140-387.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022022.

PTM databases

iPTMnetiQ5XIC0.
PhosphoSiteiQ5XIC0.

Proteomic databases

PaxDbiQ5XIC0.
PRIDEiQ5XIC0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022022; ENSRNOP00000022022; ENSRNOG00000029549. [Q5XIC0-1]
GeneIDi291075.
KEGGirno:291075.
UCSCiRGD:1359427. rat. [Q5XIC0-1]

Organism-specific databases

CTDi10455.
RGDi1359427. Eci2.

Phylogenomic databases

eggNOGiKOG0016. Eukaryota.
KOG0817. Eukaryota.
COG1024. LUCA.
COG4281. LUCA.
GeneTreeiENSGT00760000119100.
InParanoidiQ5XIC0.
KOiK13239.
OMAiKETARQN.
OrthoDBiEOG72RN06.
PhylomeDBiQ5XIC0.
TreeFamiTF313375.

Enzyme and pathway databases

SABIO-RKQ5XIC0.

Miscellaneous databases

NextBioi632071.
PROiQ5XIC0.

Gene expression databases

ExpressionAtlasiQ5XIC0. baseline and differential.
GenevisibleiQ5XIC0. RN.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
3.90.226.10. 1 hit.
InterProiIPR022408. Acyl-CoA-binding_prot_CS.
IPR000582. Acyl-CoA-binding_protein.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
[Graphical view]
PfamiPF00887. ACBP. 1 hit.
PF00378. ECH_1. 1 hit.
[Graphical view]
PRINTSiPR00689. ACOABINDINGP.
SUPFAMiSSF47027. SSF47027. 1 hit.
SSF52096. SSF52096. 1 hit.
PROSITEiPS00880. ACB_1. 1 hit.
PS51228. ACB_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  2. "Functional characterization of delta3,delta2-enoyl-CoA isomerases from rat liver."
    Zhang D., Yu W., Geisbrecht B.V., Gould S.J., Sprecher H., Schulz H.
    J. Biol. Chem. 277:9127-9132(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiECI2_RAT
AccessioniPrimary (citable) accession number: Q5XIC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: November 23, 2004
Last modified: February 17, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.