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Protein

Sphingomyelin phosphodiesterase

Gene

Smpd1

Organism
Rattus norvegicus (Rat)
Status
Unreviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts sphingomyelin to ceramide.UniRule annotation

Catalytic activityi

Sphingomyelin + H2O = N-acylsphingosine + phosphocholine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi204Zinc 1UniRule annotation1
Metal bindingi206Zinc 1; via tele nitrogenUniRule annotation1
Metal bindingi276Zinc 1UniRule annotation1
Metal bindingi276Zinc 2UniRule annotation1
Metal bindingi316Zinc 2UniRule annotation1
Metal bindingi423Zinc 2; via tele nitrogenUniRule annotation1
Metal bindingi455Zinc 2; via pros nitrogenUniRule annotation1
Metal bindingi457Zinc 1; via tele nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • ceramide biosynthetic process Source: RGD
  • negative regulation of MAP kinase activity Source: Ensembl
  • positive regulation of apoptotic process Source: RGD
  • positive regulation of protein dephosphorylation Source: Ensembl
  • response to cocaine Source: RGD
  • response to drug Source: RGD
  • sphingomyelin catabolic process Source: UniProtKB-UniRule
  • termination of signal transduction Source: Ensembl

Keywordsi

Molecular functionGlycosidaseUniRule annotation, Hydrolase
LigandMetal-bindingUniRule annotation, ZincUniRule annotation

Enzyme and pathway databases

ReactomeiR-RNO-1660662. Glycosphingolipid metabolism.
SABIO-RKiQ5XIA6.

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingomyelin phosphodiesteraseUniRule annotation (EC:3.1.4.12UniRule annotation)
Gene namesi
Name:Smpd1Imported
ORF Names:rCG_39318Imported
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1549719. Smpd1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei30 – 53HelicalSequence analysisAdd BLAST24

Keywords - Cellular componenti

Membrane

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi87 ↔ 163UniRule annotation
Disulfide bondi90 ↔ 155UniRule annotation
Disulfide bondi118 ↔ 129UniRule annotation
Disulfide bondi219 ↔ 224UniRule annotation
Disulfide bondi225 ↔ 248UniRule annotation
Disulfide bondi383 ↔ 429UniRule annotation
Disulfide bondi582 ↔ 586UniRule annotation
Disulfide bondi592 ↔ 605UniRule annotation

Keywords - PTMi

Disulfide bondUniRule annotation

Expressioni

Gene expression databases

BgeeiENSRNOG00000017977.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024224.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini83 – 167Saposin B-typeInterPro annotationAdd BLAST85

Sequence similaritiesi

Belongs to the acid sphingomyelinase family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helixSequence analysis

Phylogenomic databases

eggNOGiKOG3770. Eukaryota.
ENOG410YYPB. LUCA.
GeneTreeiENSGT00530000063095.
HOGENOMiHOG000008599.
HOVERGENiHBG004288.
KOiK12350.
OMAiADPLCCR.
OrthoDBiEOG091G03M6.
TreeFamiTF313674.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiView protein in InterPro
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
IPR011160. Sphingomy_PDE.
PfamiView protein in Pfam
PF00149. Metallophos. 1 hit.
PIRSFiPIRSF000948. Sphingomy_PDE. 1 hit.
SMARTiView protein in SMART
SM00741. SapB. 1 hit.
SUPFAMiSSF47862. SSF47862. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiView protein in PROSITE
PS50015. SAP_B. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5XIA6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHHGASSGQ GHLRAGWEQR LERSLPAPKL GLLWMGLGLA LVLALFDSTV
60 70 80 90 100
LWVPARAYPL PSQGHSVKFS AVAPQLQNAF GWRNLTCPAC KVLFTALNYG
110 120 130 140 150
LKKEPNVARV GSVAIKMCKM LNIAPLNVCQ SAVHLFEDDV VDVWTRSVLS
160 170 180 190 200
PSEACGLLLG SSCGHWDIFS TWNISLPSVP KPPPKPPSPP APGAPVSRVL
210 220 230 240 250
FLTDLHWDHD YLEGTDPNCA DPLCCRRGSG WPPNSRTGAG YWGEYSKCDL
260 270 280 290 300
PLRTLESLLK GLGPAGPFEM VYWTGDIPAH DVWQQSRQDQ LRALNTITDL
310 320 330 340 350
VWKFLGPVPV YPAVGNHEST PVNGFPPPFI KGNQSSQWLY EAMAKAWEPW
360 370 380 390 400
LPADALHTLR IGGFYALTPR PGLRLISLNM NFCSRENFWL LINSTDPAGQ
410 420 430 440 450
LQWLVDELQA AENRGDKVHI IGHIPPGHCL KSWSWNYYKI IARYENTLAG
460 470 480 490 500
QFFGHTHVDE FEIFYDEETL SRPLAVAFLG PSATTFINLN PGYRVYQIDG
510 520 530 540 550
NYPGSSHVVL DHETYILNLT QANAPGGTPS WKRLYRARET YGLPDAMPAS
560 570 580 590 600
WHNLVYRMRA DEQLFQTFWF LYHKGHPPSE PCGTPCRLAT LCAQLSARAD
610 620
SPALCRHLMP NGSLPDAHSL WSRPLLC
Length:627
Mass (Da):69,761
Last modified:November 23, 2004 - v1
Checksum:i2BE1585CF13F454C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC097992 Genomic DNA. No translation available.
BC083780 mRNA. Translation: AAH83780.1.
CH473956 Genomic DNA. Translation: EDM18044.1.
RefSeqiNP_001006998.1. NM_001006997.1.
UniGeneiRn.18277.

Genome annotation databases

EnsembliENSRNOT00000024224; ENSRNOP00000024224; ENSRNOG00000017977.
GeneIDi308909.
KEGGirno:308909.
UCSCiRGD:1549719. rat.

Similar proteinsi

Entry informationi

Entry nameiQ5XIA6_RAT
AccessioniPrimary (citable) accession number: Q5XIA6
Entry historyiIntegrated into UniProtKB/TrEMBL: November 23, 2004
Last sequence update: November 23, 2004
Last modified: September 27, 2017
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported