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Protein

Rho GDP-dissociation inhibitor 1

Gene

Arhgdia

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1.1 Publication

GO - Molecular functioni

  1. fatty acid binding Source: RGD
  2. GTPase activator activity Source: UniProtKB-KW
  3. Hsp90 protein binding Source: RGD
  4. protein kinase binding Source: RGD
  5. Rho GDP-dissociation inhibitor activity Source: Ensembl
  6. Rho GTPase binding Source: RGD

GO - Biological processi

  1. cellular response to mechanical stimulus Source: RGD
  2. cellular response to organic cyclic compound Source: RGD
  3. cellular response to redox state Source: RGD
  4. negative regulation of cell migration Source: UniProtKB
  5. regulation of cell shape Source: RGD
  6. regulation of protein localization Source: Ensembl
  7. Rho protein signal transduction Source: Ensembl
  8. semaphorin-plexin signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiREACT_197601. Axonal growth stimulation.
REACT_255528. Rho GTPase cycle.
REACT_260622. Axonal growth inhibition (RHOA activation).

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GDP-dissociation inhibitor 1By similarity
Short name:
Rho GDI 1By similarity
Alternative name(s):
Rho-GDI alphaBy similarity
Gene namesi
Name:ArhgdiaImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi1359547. Arhgdia.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosol Source: RGD
  2. extracellular space Source: RGD
  3. extracellular vesicular exosome Source: Ensembl
  4. immunological synapse Source: Ensembl
  5. membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 204203Rho GDP-dissociation inhibitor 1By similarityPRO_0000349124Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei34 – 341PhosphoserineBy similarity
Modified residuei43 – 431N6-acetyllysineBy similarity
Modified residuei105 – 1051N6-acetyllysineBy similarity
Modified residuei127 – 1271N6-acetyllysineBy similarity
Modified residuei141 – 1411N6-acetyllysine; alternateBy similarity
Modified residuei141 – 1411N6-succinyllysine; alternateBy similarity
Modified residuei178 – 1781N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5XI73.
PRIDEiQ5XI73.

2D gel databases

World-2DPAGE0004:Q5XI73.

PTM databases

PhosphoSiteiQ5XI73.

Expressioni

Gene expression databases

GenevestigatoriQ5XI73.

Interactioni

Subunit structurei

Monomer. Interacts with FER. Interacts with PLXNB3. Forms a heterodimer with RAC1. Interacts with RHOA, the affinity is increased by three orders of magnitude when RHOA is prenylated. Interacts with PSMD10; the interaction increases ARHGDIA association with RHOA, leading to ARHGDIA-mediated inactivation of RHOA and ROCK and prolonged AKT activation. Interacts with RHOC and CDC42.1 Publication

Protein-protein interaction databases

BioGridi262122. 3 interactions.
IntActiQ5XI73. 2 interactions.
STRINGi10116.ENSRNOP00000051840.

Structurei

3D structure databases

ProteinModelPortaliQ5XI73.
SMRiQ5XI73. Positions 5-204.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Rho GDI family.Sequence Analysis

Phylogenomic databases

eggNOGiNOG289931.
GeneTreeiENSGT00390000006233.
HOGENOMiHOG000175765.
HOVERGENiHBG000206.
InParanoidiQ5XI73.
KOiK12462.
OMAiWEWSLTI.
OrthoDBiEOG72JWH9.
PhylomeDBiQ5XI73.
TreeFamiTF105387.

Family and domain databases

Gene3Di2.70.50.30. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR000406. Rho_GDI.
IPR024792. RhoGDI_domain.
[Graphical view]
PANTHERiPTHR10980. PTHR10980. 1 hit.
PfamiPF02115. Rho_GDI. 1 hit.
[Graphical view]
PRINTSiPR00492. RHOGDI.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5XI73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEQEPTAEQ LAQIAAENEE DEHSVNYKPP AQKSIQEIQE LDKDDESLRK
60 70 80 90 100
YKEALLGRVA VSADPNVPNV IVTRLTLVCS TAPGPLELDL TGDLESFKKQ
110 120 130 140 150
SFVLKEGVEY RIKISFRVNR EIVSGMKYIQ HTYRKGVKID KTDYMVGSYG
160 170 180 190 200
PRAEEYEFLT PMEEAPKGML ARGSYNIKSR FTDDDKTDHL SWEWNLTIKK

EWKD
Length:204
Mass (Da):23,407
Last modified:November 23, 2004 - v1
Checksum:i8ACE6F4456D842D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083817 mRNA. Translation: AAH83817.1.
RefSeqiNP_001007006.1. NM_001007005.1.
XP_006247959.1. XM_006247897.2.
XP_006247960.1. XM_006247898.2.
UniGeneiRn.1801.

Genome annotation databases

EnsembliENSRNOT00000054957; ENSRNOP00000051840; ENSRNOG00000036688.
GeneIDi360678.
KEGGirno:360678.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083817 mRNA. Translation: AAH83817.1.
RefSeqiNP_001007006.1. NM_001007005.1.
XP_006247959.1. XM_006247897.2.
XP_006247960.1. XM_006247898.2.
UniGeneiRn.1801.

3D structure databases

ProteinModelPortaliQ5XI73.
SMRiQ5XI73. Positions 5-204.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi262122. 3 interactions.
IntActiQ5XI73. 2 interactions.
STRINGi10116.ENSRNOP00000051840.

PTM databases

PhosphoSiteiQ5XI73.

2D gel databases

World-2DPAGE0004:Q5XI73.

Proteomic databases

PaxDbiQ5XI73.
PRIDEiQ5XI73.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000054957; ENSRNOP00000051840; ENSRNOG00000036688.
GeneIDi360678.
KEGGirno:360678.

Organism-specific databases

CTDi396.
RGDi1359547. Arhgdia.

Phylogenomic databases

eggNOGiNOG289931.
GeneTreeiENSGT00390000006233.
HOGENOMiHOG000175765.
HOVERGENiHBG000206.
InParanoidiQ5XI73.
KOiK12462.
OMAiWEWSLTI.
OrthoDBiEOG72JWH9.
PhylomeDBiQ5XI73.
TreeFamiTF105387.

Enzyme and pathway databases

ReactomeiREACT_197601. Axonal growth stimulation.
REACT_255528. Rho GTPase cycle.
REACT_260622. Axonal growth inhibition (RHOA activation).

Miscellaneous databases

NextBioi673734.
PROiQ5XI73.

Gene expression databases

GenevestigatoriQ5XI73.

Family and domain databases

Gene3Di2.70.50.30. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR000406. Rho_GDI.
IPR024792. RhoGDI_domain.
[Graphical view]
PANTHERiPTHR10980. PTHR10980. 1 hit.
PfamiPF02115. Rho_GDI. 1 hit.
[Graphical view]
PRINTSiPR00492. RHOGDI.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Brown Norway1 Publication.
    Tissue: KidneyImported.
  2. "Proteome profile of the mature rat olfactory bulb."
    Maurya D.K., Sundaram C.S., Bhargava P.
    Proteomics 9:2593-2599(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
  3. "Semaphorin 5A and plexin-B3 inhibit human glioma cell motility through RhoGDIalpha-mediated inactivation of Rac1 GTPase."
    Li X., Lee A.Y.
    J. Biol. Chem. 285:32436-32445(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PLXNB3 AND RAC1.

Entry informationi

Entry nameiGDIR1_RAT
AccessioniPrimary (citable) accession number: Q5XI73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: November 23, 2004
Last modified: March 4, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.