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Q5XI73 (GDIR1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rho GDP-dissociation inhibitor 1

Short name=Rho GDI 1
Alternative name(s):
Rho-GDI alpha
Gene names
Name:Arhgdia
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length204 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1. Ref.3

Subunit structure

Monomer. Interacts with FER. Interacts with PLXNB3. Forms a heterodimer with RAC1. Interacts with RHOA, the affinity is increased by three orders of magnitude when RHOA is prenylated. Interacts with PSMD10; the interaction increases ARHGDIA association with RHOA, leading to ARHGDIA-mediated inactivation of RHOA and ROCK and prolonged AKT activation. Interacts with RHOC and CDC42. Ref.3

Subcellular location

Cytoplasm Ref.2.

Sequence similarities

Belongs to the Rho GDI family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity UniProtKB P52565
Chain2 – 204203Rho GDP-dissociation inhibitor 1 UniProtKB P52565
PRO_0000349124

Amino acid modifications

Modified residue21N-acetylalanine By similarity UniProtKB P52565
Modified residue341Phosphoserine By similarity
Modified residue431N6-acetyllysine By similarity
Modified residue1051N6-acetyllysine By similarity
Modified residue1271N6-acetyllysine By similarity
Modified residue1411N6-acetyllysine; alternate By similarity UniProtKB P52565
Modified residue1411N6-succinyllysine; alternate By similarity
Modified residue1781N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5XI73 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 8ACE6F4456D842D8

FASTA20423,407
        10         20         30         40         50         60 
MAEQEPTAEQ LAQIAAENEE DEHSVNYKPP AQKSIQEIQE LDKDDESLRK YKEALLGRVA 

        70         80         90        100        110        120 
VSADPNVPNV IVTRLTLVCS TAPGPLELDL TGDLESFKKQ SFVLKEGVEY RIKISFRVNR 

       130        140        150        160        170        180 
EIVSGMKYIQ HTYRKGVKID KTDYMVGSYG PRAEEYEFLT PMEEAPKGML ARGSYNIKSR 

       190        200 
FTDDDKTDHL SWEWNLTIKK EWKD 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Brown Norway.
Tissue: Kidney.
[2]"Proteome profile of the mature rat olfactory bulb."
Maurya D.K., Sundaram C.S., Bhargava P.
Proteomics 9:2593-2599(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[3]"Semaphorin 5A and plexin-B3 inhibit human glioma cell motility through RhoGDIalpha-mediated inactivation of Rac1 GTPase."
Li X., Lee A.Y.
J. Biol. Chem. 285:32436-32445(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PLXNB3 AND RAC1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC083817 mRNA. Translation: AAH83817.1.
RefSeqNP_001007006.1. NM_001007005.1.
XP_006247959.1. XM_006247897.1.
XP_006247960.1. XM_006247898.1.
UniGeneRn.1801.

3D structure databases

ProteinModelPortalQ5XI73.
SMRQ5XI73. Positions 5-204.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid262122. 3 interactions.
IntActQ5XI73. 2 interactions.
STRING10116.ENSRNOP00000051840.

PTM databases

PhosphoSiteQ5XI73.

2D gel databases

World-2DPAGE0004:Q5XI73.

Proteomic databases

PaxDbQ5XI73.
PRIDEQ5XI73.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000054957; ENSRNOP00000051840; ENSRNOG00000036688.
GeneID360678.
KEGGrno:360678.

Organism-specific databases

CTD396.
RGD1359547. Arhgdia.

Phylogenomic databases

eggNOGNOG289931.
GeneTreeENSGT00390000006233.
HOGENOMHOG000175765.
HOVERGENHBG000206.
InParanoidQ5XI73.
KOK12462.
OMAEGVEYQI.
OrthoDBEOG72JWH9.
PhylomeDBQ5XI73.
TreeFamTF105387.

Gene expression databases

GenevestigatorQ5XI73.

Family and domain databases

Gene3D2.70.50.30. 1 hit.
InterProIPR014756. Ig_E-set.
IPR000406. Rho_GDI.
IPR024792. RhoGDI_domain.
[Graphical view]
PANTHERPTHR10980. PTHR10980. 1 hit.
PfamPF02115. Rho_GDI. 1 hit.
[Graphical view]
PRINTSPR00492. RHOGDI.
SUPFAMSSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

NextBio673734.
PROQ5XI73.

Entry information

Entry nameGDIR1_RAT
AccessionPrimary (citable) accession number: Q5XI73
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: November 23, 2004
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families