Q5XI22 (THIC_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-CoA acetyltransferase, cytosolic
EC=2.3.1.9

Alternative name(s):
Cytosolic acetoacetyl-CoA thiolase

Gene names

Name:

Acat2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	2 acetyl-CoA = CoA + acetoacetyl-CoA.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	brain development Inferred from expression pattern. Source: RGD cellular response to fatty acid Inferred from expression pattern. Source: RGD cellular response to nutrient Inferred from expression pattern. Source: RGD ketone body catabolic process Inferred from expression pattern. Source: RGD liver development Inferred from expression pattern. Source: RGD
Cellular component	cytosol Inferred from direct assay. Source: RGD
Molecular function	acetyl-CoA C-acetyltransferase activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	Acetyl-CoA acetyltransferase, cytosolic		PRO_0000271366
Sites
	Active site	92	1	Acyl-thioester intermediate By similarity
	Active site	353	1	Proton acceptor By similarity
	Active site	383	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	200	1	N6-acetyllysine By similarity
	Modified residue	233	1	N6-acetyllysine By similarity
	Modified residue	235	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5XI22 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 850589DD5AF4D84F	FASTA	397	41,108
	10 20 30 40 50 60 MNAGSDPVVI ISAARTAIGS FNGALSTVPV HNLGTTVIKE VLQRAKVAPE EVSEVIFGHV 70 80 90 100 110 120 LTAGCGQNPT RQASVGAGIP YSVPAWSCQM ICGSGLKAVC LAAQSIAMGD STIVVAGGME 130 140 150 160 170 180 NMSKAPHLAH LRSGVKMGEV PLADSILCDG LTDAFHNYHM GITAENVAKK WQVSREAQDK 190 200 210 220 230 240 VAVVSQNRAE HAQKAGHFDK EIVPVHVSSR KGLTEVKIDE FPRHGSNLEA MSKLKPYFLT 250 260 270 280 290 300 DGTGTVTPAN ASGMNDGAAA VVLMKKTEAE SRMLKPLAQV VSWSQAGVEP SVMGVGPIPA 310 320 330 340 350 360 IKQAVAKAGW SLEDVDVFEI NEAFAAVSAA IAKELGLSPE KVNIDGGAIA LGHPLGASGC 370 380 390 RILVTLLHTL ERVGGTRGVA ALCIGGGMGI AMCVQRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[2]	Lubec G., Afjehi-Sadat L., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 16-39; 195-210; 218-223 AND 342-372, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus and Spinal cord.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC083872 mRNA. Translation: AAH83872.1.
IPI	IPI00480766.
RefSeq	NP_001006996.1. NM_001006995.1.
UniGene	Rn.203063.
3D structure databases
HSSP	HSSP built from PDB template 1WL4 based on UniProtKB Q9BWD1.
ProteinModelPortal	Q5XI22.
SMR	Q5XI22. Positions 4-396.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5XI22.
PTM databases
PhosphoSite	Q5XI22.
Proteomic databases
PRIDE	Q5XI22.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000026003; ENSRNOP00000026003; ENSRNOG00000019189.
GeneID	308100.
KEGG	rno:308100.
NMPDR	fig|10116.3.peg.448.
UCSC	NM_001006995. rat.
Organism-specific databases
CTD	224530.
RGD	1359366. Acat2.
Phylogenomic databases
eggNOG	roNOG04002.
GeneTree	ENSGT00390000009412.
HOVERGEN	HBG003112.
InParanoid	Q5XI22.
OMA	KKSEAAN.
PhylomeDB	Q5XI22.
Gene expression databases
ArrayExpress	Q5XI22.
Genevestigator	Q5XI22.
Family and domain databases
InterPro	IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020610. Thiolase_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 4 hits.
KO	K00626.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. False negative. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	658342.

Entry information

Entry name

THIC_RAT

Accession

Primary (citable) accession number: Q5XI22

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	November 23, 2004
Last modified:	November 16, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program