Q5XI06 (KAT8_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone acetyltransferase KAT8
EC=2.3.1.48

Alternative name(s):
Lysine acetyltransferase 8
MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1
Short name=MYST-1

Gene names

Name:	Kat8
Synonyms:	Myst1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	458 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Histone acetyltransferase which may be involved in transcriptional activation. May influence the function of ATM. As part of the MSL complex it is involved in acetylation of nucleosomal histone H4 producing specifically H4K16ac. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. That activity is less specific than the one of the MSL complex By similarity.
Catalytic activity	Acetyl-CoA + [histone] = CoA + acetyl-[histone].
Subunit structure	Component of a multisubunit histone acetyltransferase complex (MSL) at least composed of the MOF/KAT8, MSL1/hampin, MSL2L1 and MSL3L1. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MOF/KAT8, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with the chromodomain of MORF4L1/MRG15. Interacts with ATM through the chromodomain By similarity.
Subcellular location	Nucleus. Chromosome By similarity.
Post-translational modification	Autoacetylation at Lys-274 is required for proper function By similarity.
Sequence similarities	Belongs to the MYST (SAS/MOZ) family. Contains 1 C2HC-type zinc finger. Contains 1 chromo domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Chromosome Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Activator Acyltransferase Chromatin regulator Transferase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histone H4-K16 acetylation Inferred from sequence or structural similarity. Source: UniProtKB histone H4-K5 acetylation Inferred from sequence or structural similarity. Source: UniProtKB histone H4-K8 acetylation Inferred from sequence or structural similarity. Source: UniProtKB myeloid cell differentiation Inferred from electronic annotation. Source: Compara negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	MLL1 complex Inferred from sequence or structural similarity. Source: UniProtKB MSL complex Inferred from sequence or structural similarity. Source: UniProtKB kinetochore Inferred from electronic annotation. Source: Compara
Molecular_function	histone acetyltransferase activity (H4-K16 specific) Inferred from electronic annotation. Source: Compara histone acetyltransferase activity (H4-K5 specific) Inferred from electronic annotation. Source: Compara histone acetyltransferase activity (H4-K8 specific) Inferred from electronic annotation. Source: Compara metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 458	458	Histone acetyltransferase KAT8		PRO_0000051568
Regions
	Domain	69 – 123	55	Chromo
	Zinc finger	208 – 230	23	C2HC-type
	Region	174 – 458	285	Sufficient for interaction with KANSL1 By similarity
	Region	324 – 330	7	Acetyl-CoA binding By similarity
Sites
	Active site	274	1	By similarity
	Active site	316	1	Nucleophile By similarity
	Binding site	319	1	Acetyl-CoA By similarity
	Binding site	354	1	Acetyl-CoA By similarity
Amino acid modifications
	Modified residue	37	1	Phosphoserine By similarity
	Modified residue	42	1	Phosphoserine By similarity
	Modified residue	113	1	N6-acetyllysine By similarity
	Modified residue	274	1	N6-acetyllysine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5XI06 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 0D3AFF8A5EDB1459	FASTA	458	52,632
	10 20 30 40 50 60 MAAQGATAAV AATTSGIVGE GEPGPGENTS VEGPARSPGR VSPPTPARGE PEVTVEIGET 70 80 90 100 110 120 YLCRRPDSTW HSAEVIQSRV NDQEGREEFY VHYVGFNRRL DEWVDKNRLA LTKTVKDAVQ 130 140 150 160 170 180 KNSEKYLSEL AEQPERKITR NQKRKHDEIN HVQKTYAEMD PTTAALEKEH EAITKVKYVD 190 200 210 220 230 240 KIHIGNYEID AWYFSPFPED YGKQPKLWLC EYCLKYMKFE KSYRFHLGQC QWRQPPGKEI 250 260 270 280 290 300 YRKSNISVYE VDGKDHKIYC QNLCLLAKLF LDHKTLYFDV EPFVFYILTE VDRQGAHIVG 310 320 330 340 350 360 YFSKEKESPD GNNVACILTL PPYQRRGYGK FLIAFSYELS KLESTVGSPE KPLSDLGKLS 370 380 390 400 410 420 YRSYWSWVLL EILRDFRGTL SIKDLSQMTS ITQNDIISTL QSLNMVKYWK GQHVICVTPK 430 440 450 LVEEHLKSAQ YKKPPITVDS VCLKWAPPKH KQVKLSKK

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC083891 mRNA. Translation: AAH83891.1.
IPI	IPI00199110.
RefSeq	NP_001017378.1. NM_001017378.1.
UniGene	Rn.205579.
3D structure databases
ProteinModelPortal	Q5XI06.
SMR	Q5XI06. Positions 47-169, 177-447.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000026527.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000026527; ENSRNOP00000026527; ENSRNOG00000019585.
GeneID	310194.
KEGG	rno:310194.
UCSC	RGD:1311512. rat.
Organism-specific databases
CTD	84148.
RGD	1311512. Kat8.
Phylogenomic databases
eggNOG	COG5027.
GeneTree	ENSGT00550000074503.
HOGENOM	HOG000182457.
HOVERGEN	HBG053268.
InParanoid	Q5XI06.
KO	K11308.
OMA	DGRDHKI.
OrthoDB	EOG4933HQ.
Gene expression databases
ArrayExpress	Q5XI06.
Genevestigator	Q5XI06.
Family and domain databases
Gene3D	3.40.630.30. 1 hit.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000953. Chromo_domain/shadow. IPR016197. Chromodomain-like. IPR002717. MOZ_SAS. IPR025995. Tudor-knot. [Graphical view]
Pfam	PF01853. MOZ_SAS. 1 hit. PF11717. Tudor-knot. 1 hit. [Graphical view]
SMART	SM00298. CHROMO. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit. SSF54160. Chromodomain-like. 1 hit.
PROSITE	PS00598. CHROMO_1. False negative. PS50013. CHROMO_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	661703.

Entry information

Entry name

KAT8_RAT

Accession

Primary (citable) accession number: Q5XI06

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	November 23, 2004
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program