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Q5XI06 (KAT8_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histone acetyltransferase KAT8

EC=2.3.1.48
Alternative name(s):
Lysine acetyltransferase 8
MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1
Short name=MYST-1
Gene names
Name:Kat8
Synonyms:Myst1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length458 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Histone acetyltransferase which may be involved in transcriptional activation. May influence the function of ATM. As part of the MSL complex it is involved in acetylation of nucleosomal histone H4 producing specifically H4K16ac. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. That activity is less specific than the one of the MSL complex By similarity.

Catalytic activity

Acetyl-CoA + [histone] = CoA + acetyl-[histone].

Subunit structure

Component of a multisubunit histone acetyltransferase complex (MSL) at least composed of the MOF/KAT8, MSL1/hampin, MSL2L1 and MSL3L1. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Interacts with KANSL1; the interaction is direct. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MOF/KAT8, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with the chromodomain of MORF4L1/MRG15. Interacts with ATM through the chromodomain By similarity.

Subcellular location

Nucleus. Chromosome By similarity.

Post-translational modification

Autoacetylation at Lys-274 is required for proper function By similarity.

Sequence similarities

Belongs to the MYST (SAS/MOZ) family.

Contains 1 C2HC-type zinc finger.

Contains 1 chromo domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentChromosome
Nucleus
   DomainZinc-finger
   LigandMetal-binding
Zinc
   Molecular functionActivator
Acyltransferase
Chromatin regulator
Transferase
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processhistone H4-K16 acetylation

Inferred from sequence or structural similarity. Source: UniProtKB

histone H4-K5 acetylation

Inferred from sequence or structural similarity. Source: UniProtKB

histone H4-K8 acetylation

Inferred from sequence or structural similarity. Source: UniProtKB

myeloid cell differentiation

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentMLL1 complex

Inferred from sequence or structural similarity. Source: UniProtKB

MSL complex

Inferred from sequence or structural similarity. Source: UniProtKB

histone acetyltransferase complex

Inferred from sequence or structural similarity. Source: UniProtKB

kinetochore

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionhistone acetyltransferase activity (H4-K16 specific)

Inferred from electronic annotation. Source: Ensembl

histone acetyltransferase activity (H4-K5 specific)

Inferred from electronic annotation. Source: Ensembl

histone acetyltransferase activity (H4-K8 specific)

Inferred from electronic annotation. Source: Ensembl

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 458457Histone acetyltransferase KAT8
PRO_0000051568

Regions

Domain69 – 12355Chromo
Zinc finger208 – 23023C2HC-type
Region174 – 458285Sufficient for interaction with KANSL1 By similarity
Region324 – 3307Acetyl-CoA binding By similarity

Sites

Active site2741 By similarity
Active site3501Proton donor/acceptor By similarity
Binding site3191Acetyl-CoA By similarity
Binding site3541Acetyl-CoA By similarity
Binding site3631Acetyl-CoA By similarity
Binding site4321Acetyl-CoA By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue1131N6-acetyllysine By similarity
Modified residue2741N6-acetyllysine; by autocatalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5XI06 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 0D3AFF8A5EDB1459

FASTA45852,632
        10         20         30         40         50         60 
MAAQGATAAV AATTSGIVGE GEPGPGENTS VEGPARSPGR VSPPTPARGE PEVTVEIGET 

        70         80         90        100        110        120 
YLCRRPDSTW HSAEVIQSRV NDQEGREEFY VHYVGFNRRL DEWVDKNRLA LTKTVKDAVQ 

       130        140        150        160        170        180 
KNSEKYLSEL AEQPERKITR NQKRKHDEIN HVQKTYAEMD PTTAALEKEH EAITKVKYVD 

       190        200        210        220        230        240 
KIHIGNYEID AWYFSPFPED YGKQPKLWLC EYCLKYMKFE KSYRFHLGQC QWRQPPGKEI 

       250        260        270        280        290        300 
YRKSNISVYE VDGKDHKIYC QNLCLLAKLF LDHKTLYFDV EPFVFYILTE VDRQGAHIVG 

       310        320        330        340        350        360 
YFSKEKESPD GNNVACILTL PPYQRRGYGK FLIAFSYELS KLESTVGSPE KPLSDLGKLS 

       370        380        390        400        410        420 
YRSYWSWVLL EILRDFRGTL SIKDLSQMTS ITQNDIISTL QSLNMVKYWK GQHVICVTPK 

       430        440        450 
LVEEHLKSAQ YKKPPITVDS VCLKWAPPKH KQVKLSKK 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC083891 mRNA. Translation: AAH83891.1.
RefSeqNP_001017378.1. NM_001017378.1.
UniGeneRn.205579.

3D structure databases

ProteinModelPortalQ5XI06.
SMRQ5XI06. Positions 47-169, 177-447.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000026527.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000026527; ENSRNOP00000026527; ENSRNOG00000019585.
GeneID310194.
KEGGrno:310194.
UCSCRGD:1311512. rat.

Organism-specific databases

CTD84148.
RGD1311512. Kat8.

Phylogenomic databases

eggNOGCOG5027.
GeneTreeENSGT00550000074503.
HOGENOMHOG000182457.
HOVERGENHBG053268.
InParanoidQ5XI06.
KOK11308.
OMADGRDHKI.
OrthoDBEOG7WHH8N.
PhylomeDBQ5XI06.
TreeFamTF317619.

Gene expression databases

GenevestigatorQ5XI06.

Family and domain databases

Gene3D3.40.630.30. 1 hit.
InterProIPR016181. Acyl_CoA_acyltransferase.
IPR000953. Chromo_domain/shadow.
IPR016197. Chromodomain-like.
IPR002717. MOZ_SAS.
IPR025995. Tudor-knot.
[Graphical view]
PfamPF01853. MOZ_SAS. 1 hit.
PF11717. Tudor-knot. 1 hit.
[Graphical view]
SMARTSM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMSSF54160. SSF54160. 1 hit.
SSF55729. SSF55729. 1 hit.
ProtoNetSearch...

Other

NextBio661703.
PROQ5XI06.

Entry information

Entry nameKAT8_RAT
AccessionPrimary (citable) accession number: Q5XI06
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families