Q5XI02 (PDILT_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein disulfide-isomerase-like protein of the testis

Gene names

Name:

Pdilt

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable redox-inactive chaperone involved in spermatogenesis By similarity.
Subunit structure	Homodimer. The homodimer is not disulfide-linked. Interacts with ERO1L and CLGN By similarity.
Subcellular location	Endoplasmic reticulum By similarity.
Tissue specificity	Testis-specific. Expressed exclusively in postmeiotic male germ cells (at protein level). Ref.2
Developmental stage	Induced during puberty. Ref.2
Domain	The thioredoxin domain lacks the conserved redox-active Cys at position 414 which is replaced by a Ser residue, suggesting that it lacks thioredoxin activity.
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 1 thioredoxin domain.

Ontologies

Keywords
Biological process	Differentiation Spermatogenesis
Cellular component	Endoplasmic reticulum
Domain	Signal
Molecular function	Chaperone Developmental protein Isomerase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW cell redox homeostasis Inferred from electronic annotation. Source: InterPro multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW spermatogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 590	570	Protein disulfide-isomerase-like protein of the testis		PRO_0000325852
Regions
	Domain	385 – 448	64	Thioredoxin
	Motif	587 – 590	4	Prevents secretion from ER Potential
Amino acid modifications
	Glycosylation	55	1	N-linked (GlcNAc...) Potential
	Glycosylation	337	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5XI02 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 6F73FA1B8CD304DD	FASTA	590	68,267
	10 20 30 40 50 60 MELLWTPLLL LAACLSEVLG SPEMDTGINI SQPLHILEDH NLMVLTPAGL TQTLNETRFL 70 80 90 100 110 120 MVIFHNPTLK QSRKLAKELG KAAEIFGKGK NGLGFGKVDI TMETELKQEF DITHAPELKL 130 140 150 160 170 180 FYEGNRLEPI SCKDVVESTA LVVWLRRQIS KKALLFNNSN EVADFVKSRP LVIVGFFQDL 190 200 210 220 230 240 EEEVAELFYD TIKDFPELTF GAIQIKNSFG RFHVILDSVL VFKKGRVVKR QELINDSTNK 250 260 270 280 290 300 DYLNQVIKQQ LTGFVIEFNP ENKDLIYEMN ILNHMLLFIS KNSEPYSTII RHYRQISKEF 310 320 330 340 350 360 QNKILFVLVN SDEPKNKRIF EYFQISRVNV PSVQILNLSS DARYKMPTDN ITFESLKKFC 370 380 390 400 410 420 NSFLSRTAKK HKSSEEIPKY WDQEPVKKLV GKNFNVVVFD KEKDVFVMFY APWSEKCRVL 430 440 450 460 470 480 LPLLEELGIK YQNHSTVIIA KIDITANDIQ LANPEQYPFF RLFPTDSQEA VMYKGEHTMK 490 500 510 520 530 540 GFCDFLESHV KVRIEEDDEL LYIEQNEVAE EEVLAEPEMQ HIDKLPEKPP LKVEDTSKQD 550 560 570 580 590 RPAKESPALG SISQPEELER RKETAEKEKK VAQPKEQPKP ERKLDIKEEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[2]	"A developmentally regulated chaperone complex for the endoplasmic reticulum of male haploid germ cells." van Lith M., Karala A.R., Bown D., Gatehouse J.A., Ruddock L.W., Saunders P.T.K., Benham A.M. Mol. Biol. Cell 18:2795-2804(2007) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC083897 mRNA. Translation: AAH83897.1.
IPI	IPI00208537.
RefSeq	NP_001013924.1. NM_001013902.1.
UniGene	Rn.146183.
3D structure databases
HSSP	HSSP built from PDB template 1X5C based on UniProtKB P07237.
ProteinModelPortal	Q5XI02.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000020817.
PTM databases
PhosphoSite	Q5XI02.
Proteomic databases
PRIDE	Q5XI02.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000020817; ENSRNOP00000020817; ENSRNOG00000015368.
GeneID	293544.
KEGG	rno:293544.
UCSC	RGD:1307822. rat.
Organism-specific databases
CTD	204474.
RGD	1307822. Pdilt.
Phylogenomic databases
eggNOG	COG0526.
GeneTree	ENSGT00700000104429.
HOGENOM	HOG000115479.
HOVERGEN	HBG108240.
InParanoid	Q5XI02.
OrthoDB	EOG4RFKSG.
Gene expression databases
Genevestigator	Q5XI02.
Family and domain databases
Gene3D	3.40.30.10. 3 hits.
InterPro	IPR012336. Thioredoxin-like_fold. IPR013766. Thioredoxin_domain. [Graphical view]
Pfam	PF00085. Thioredoxin. 1 hit. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 4 hits.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00194. THIOREDOXIN_1. False negative. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	636364.

Entry information

Entry name

PDILT_RAT

Accession

Primary (citable) accession number: Q5XI02

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	November 23, 2004
Last modified:	April 3, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program