Reviewed, UniProtKB/Swiss-Prot Q5XHY5 (SYTC_RAT)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:

Tars

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	695 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 695	695	Threonyl-tRNA synthetase, cytoplasmic		PRO_0000101121
Amino acid modifications
	Modified residue	215	1	N6-acetyllysine By similarity
	Modified residue	270	1	Phosphotyrosine By similarity
	Cross-link	194		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5XHY5-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: B282CF7F5F47363A	FASTA	695	80,576
	10 20 30 40 50 60 MSEEKASSPS GKMDGEKPLN PWPEYINTRL DMYHKLKAEH DSILAEKAAK DSKPIKVTLP 70 80 90 100 110 120 DGKQVDAESW KTTPYQIACG ISQGLADNTV VAKVNKVVWD LDRPLETDCT LELLKFEDEE 130 140 150 160 170 180 AQAVYWHSSA HIMGEAMERV YGGCLCYGPP IENGFYYDMY LEEGGVSSND FSSLETLCKK 190 200 210 220 230 240 IIKEKQTFER LEVKKETLLE MFKYNKFKCR ILNEKVNTPT TTVYRCGPLI DLCRGPHVRH 250 260 270 280 290 300 TGKIKTLKIH KNSSTYWEGK ADMETLQRIY GISFPDPKLL KEWEKFQEEA KNRDHRKIGR 310 320 330 340 350 360 DQELYFFHEL SPGSCFFLPK GAYIYNTLME FIRSEYRKRG FQEVVTPNIF NSRLWMTSGH 370 380 390 400 410 420 WQHYSENMFS FEVEKEQFAL KPMNCPGHCL MFDHRPRSWR ELPLRLADFG VLHRNELSGA 430 440 450 460 470 480 LTGLTRVRRF QQDDAHIFCA MEQIEDEIKG CLDFLRTVYS VFGFSFKLNL STRPEKFLGD 490 500 510 520 530 540 IEIWNQAEKQ LENSLNEFGE KWELNPGDGA FYGPKIDIQI KDAIGRYHQC ATIQLDFQLP 550 560 570 580 590 600 IRFNLTYVSH DGDDKKRPVI VHRAILGSVE RMIAILTENY GGKWPFWLSP RQVMVVPVGP 610 620 630 640 650 660 TCDEYAQKVR QEFHDAKFMV DIDLDPGCTL NKKIRNAQLA QYNFILVVGE KEKASGTVNI 670 680 690 RTRDNKVHGE RTVGETVERL QQLKQLRSKQ AEEEF

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC083914 mRNA. Translation: AAH83914.1.
IPI	IPI00559880.
RefSeq	NP_001006977.1.
UniGene	Rn.22757
3D structure databases
SMR	Q5XHY5. Positions 53-125, 55-685, 293-692.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5XHY5.
PTM databases
PhosphoSite	Q5XHY5.
Genome annotation databases
Ensembl	ENSRNOT00000051910; ENSRNOP00000044467; ENSRNOG00000019023; Rattus norvegicus. [Genome view]
GeneID	294810.
KEGG	rno:294810.
NMPDR	fig|10116.3.peg.15656.
UCSC	NM_001006976. rat.
Organism-specific databases
CTD	294810.
RGD	1359527. Tars.
Phylogenomic databases
eggNOG	roNOG15014.
HOVERGEN	Q5XHY5.
PhylomeDB	Q5XHY5.
Enzyme and pathway databases
BRENDA	6.1.1.3. 248.
Gene expression databases
ArrayExpress	Q5XHY5.
Genevestigator	Q5XHY5.
GermOnline	ENSRNOG00000019023. Rattus norvegicus.
Family and domain databases
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	638628.

Entry information

Entry name

SYTC_RAT

Accession

Primary (citable) accession number: Q5XHY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	November 23, 2004
Last modified:	February 9, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)