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Protein

Threonine--tRNA ligase, cytoplasmic

Gene

Tars

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligase, cytoplasmic (EC:6.1.1.3)
Alternative name(s):
Threonyl-tRNA synthetase
Short name:
ThrRS
Gene namesi
Name:Tars
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1359527. Tars.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 695695Threonine--tRNA ligase, cytoplasmicPRO_0000101121Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki194 – 194Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei215 – 2151N6-acetyllysineBy similarity
Modified residuei270 – 2701PhosphotyrosineCombined sources

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5XHY5.
PRIDEiQ5XHY5.

PTM databases

iPTMnetiQ5XHY5.
PhosphoSiteiQ5XHY5.

Expressioni

Gene expression databases

ExpressionAtlasiQ5XHY5. baseline and differential.
GenevisibleiQ5XHY5. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi254808. 1 interaction.
STRINGi10116.ENSRNOP00000044467.

Structurei

3D structure databases

ProteinModelPortaliQ5XHY5.
SMRiQ5XHY5. Positions 53-125.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1637. Eukaryota.
COG0441. LUCA.
GeneTreeiENSGT00390000002149.
HOGENOMiHOG000003878.
HOVERGENiHBG059513.
InParanoidiQ5XHY5.
KOiK01868.
OMAiYGEEKSE.
OrthoDBiEOG7JDQX3.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5XHY5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEEKASSPS GKMDGEKPLN PWPEYINTRL DMYHKLKAEH DSILAEKAAK
60 70 80 90 100
DSKPIKVTLP DGKQVDAESW KTTPYQIACG ISQGLADNTV VAKVNKVVWD
110 120 130 140 150
LDRPLETDCT LELLKFEDEE AQAVYWHSSA HIMGEAMERV YGGCLCYGPP
160 170 180 190 200
IENGFYYDMY LEEGGVSSND FSSLETLCKK IIKEKQTFER LEVKKETLLE
210 220 230 240 250
MFKYNKFKCR ILNEKVNTPT TTVYRCGPLI DLCRGPHVRH TGKIKTLKIH
260 270 280 290 300
KNSSTYWEGK ADMETLQRIY GISFPDPKLL KEWEKFQEEA KNRDHRKIGR
310 320 330 340 350
DQELYFFHEL SPGSCFFLPK GAYIYNTLME FIRSEYRKRG FQEVVTPNIF
360 370 380 390 400
NSRLWMTSGH WQHYSENMFS FEVEKEQFAL KPMNCPGHCL MFDHRPRSWR
410 420 430 440 450
ELPLRLADFG VLHRNELSGA LTGLTRVRRF QQDDAHIFCA MEQIEDEIKG
460 470 480 490 500
CLDFLRTVYS VFGFSFKLNL STRPEKFLGD IEIWNQAEKQ LENSLNEFGE
510 520 530 540 550
KWELNPGDGA FYGPKIDIQI KDAIGRYHQC ATIQLDFQLP IRFNLTYVSH
560 570 580 590 600
DGDDKKRPVI VHRAILGSVE RMIAILTENY GGKWPFWLSP RQVMVVPVGP
610 620 630 640 650
TCDEYAQKVR QEFHDAKFMV DIDLDPGCTL NKKIRNAQLA QYNFILVVGE
660 670 680 690
KEKASGTVNI RTRDNKVHGE RTVGETVERL QQLKQLRSKQ AEEEF
Length:695
Mass (Da):80,576
Last modified:November 23, 2004 - v1
Checksum:iB282CF7F5F47363A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083914 mRNA. Translation: AAH83914.1.
RefSeqiNP_001006977.1. NM_001006976.1.
UniGeneiRn.22757.

Genome annotation databases

EnsembliENSRNOT00000051910; ENSRNOP00000044467; ENSRNOG00000019023.
GeneIDi294810.
KEGGirno:294810.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083914 mRNA. Translation: AAH83914.1.
RefSeqiNP_001006977.1. NM_001006976.1.
UniGeneiRn.22757.

3D structure databases

ProteinModelPortaliQ5XHY5.
SMRiQ5XHY5. Positions 53-125.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi254808. 1 interaction.
STRINGi10116.ENSRNOP00000044467.

PTM databases

iPTMnetiQ5XHY5.
PhosphoSiteiQ5XHY5.

Proteomic databases

PaxDbiQ5XHY5.
PRIDEiQ5XHY5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000051910; ENSRNOP00000044467; ENSRNOG00000019023.
GeneIDi294810.
KEGGirno:294810.

Organism-specific databases

CTDi6897.
RGDi1359527. Tars.

Phylogenomic databases

eggNOGiKOG1637. Eukaryota.
COG0441. LUCA.
GeneTreeiENSGT00390000002149.
HOGENOMiHOG000003878.
HOVERGENiHBG059513.
InParanoidiQ5XHY5.
KOiK01868.
OMAiYGEEKSE.
OrthoDBiEOG7JDQX3.

Miscellaneous databases

PROiQ5XHY5.

Gene expression databases

ExpressionAtlasiQ5XHY5. baseline and differential.
GenevisibleiQ5XHY5. RN.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-270, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYTC_RAT
AccessioniPrimary (citable) accession number: Q5XHY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: November 23, 2004
Last modified: June 8, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.