Reviewed, UniProtKB/Swiss-Prot Q5XHG1 (NSMA3_XENLA)

Names and origin

Protein names

Recommended name:
    Sphingomyelin phosphodiesterase 4
    EC=3.1.4.12
Alternative name(s):
    Neutral sphingomyelinase 3
      Short name=nSMase-3
      Short name=nSMase3
    Neutral sphingomyelinase III

Gene names

Name:

smpd4

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	824 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide By similarity.
Catalytic activity	Sphingomyelin + H2O = N-acylsphingosine + choline phosphate.
Cofactor	Magnesium By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Golgi apparatus membrane; Single-pass membrane protein By similarity.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW sphingomyelin phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5XHG1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5XHG1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     289-316: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 824	824	Sphingomyelin phosphodiesterase 4		PRO_0000273166
Regions
	Transmembrane	777 – 797	21	Potential
Natural variations
	Alternative sequence	289 – 316	28	Missing in isoform 2.		VSP_022488

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 3090723AA5A777FC	FASTA	824	93,796
	10 20 30 40 50 60 MLLAGGSPQP SFLLGSLKAD CVTKPFLQRC QDLVRVIEDF PAKELHAIFP WLIETVFGSL 70 80 90 100 110 120 DGSILGWNLR GLHERINPLE FHTALEFLDP SGAMMKLVYK LQAEEYKYDF PVSFLPGPVR 130 140 150 160 170 180 ASIQERVLPE CPLYHNKIQF PASGGVSFNL ALNSFEYFMF HFAFCLLKQR NYPQGLHFST 190 200 210 220 230 240 ADSAYYILVD KYLKWFLPVE GNVPPPHSPN TGGTVPSPAP RSPSLSFTSY GSHTSLLKRH 250 260 270 280 290 300 ISHQHLVNAD PAAQEIWRTE TLLQVFVEIW LHHYSLEMYQ KMQSPNAKLE ALHNRLSVSS 310 320 330 340 350 360 APPIYPALPG SLHSYQELFQ PTEEHVLVVR LLVKHLHTFS NSIRPEQVSP STHSHTASPL 370 380 390 400 410 420 EELKRVVVPR FIQQKLYIFL QHCFGHWPLD ASFRAVLEMW LSYVQPWRYV LERSSPVSGE 430 440 450 460 470 480 MQNRNVPEKW STFVQENLLF YTKLFLRFLS RALRTDLVNP KNALMVFRAA KVFSQLNLPE 490 500 510 520 530 540 MILNGEQLFL KPEHVIPHRQ HRLLLTPNLG GSFLSSWQPP ITDTSLKVKS HVFSLEGQDC 550 560 570 580 590 600 QYMQMFGPEA RNLVLRLAQM ISQAKQTAKS ISNHSPDSSA NQSFLSWFGL GSPDFNGSYN 610 620 630 640 650 660 GSDLDEAGYD TIRKTDEHLE KALDYFCQIF RLNPTQLGQL TANVDSSQDD DGKNKLPDCI 670 680 690 700 710 720 QSEDGVVLTS LGRYQIINGL RKFDIEYQGD PELQPIRSYE NAMLVRYLYR LSSVINKRFA 730 740 750 760 770 780 NSMGALCARK DFLGKLCRHH LTSSSRKCKK SPITSVSPSE PAAPHIRLRF LASYRTLAFL 790 800 810 820 FIFYILGSLL SLGPLICTFL LLIGCMFYAI VQTLLSEEQK PHNN
	Isoform 2. Checksum: 7A4E0C21B71C7EA4	FASTA	796	90,796
	10 20 30 40 50 60 MLLAGGSPQP SFLLGSLKAD CVTKPFLQRC QDLVRVIEDF PAKELHAIFP WLIETVFGSL 70 80 90 100 110 120 DGSILGWNLR GLHERINPLE FHTALEFLDP SGAMMKLVYK LQAEEYKYDF PVSFLPGPVR 130 140 150 160 170 180 ASIQERVLPE CPLYHNKIQF PASGGVSFNL ALNSFEYFMF HFAFCLLKQR NYPQGLHFST 190 200 210 220 230 240 ADSAYYILVD KYLKWFLPVE GNVPPPHSPN TGGTVPSPAP RSPSLSFTSY GSHTSLLKRH 250 260 270 280 290 300 ISHQHLVNAD PAAQEIWRTE TLLQVFVEIW LHHYSLEMYQ KMQSPNAKEL FQPTEEHVLV 310 320 330 340 350 360 VRLLVKHLHT FSNSIRPEQV SPSTHSHTAS PLEELKRVVV PRFIQQKLYI FLQHCFGHWP 370 380 390 400 410 420 LDASFRAVLE MWLSYVQPWR YVLERSSPVS GEMQNRNVPE KWSTFVQENL LFYTKLFLRF 430 440 450 460 470 480 LSRALRTDLV NPKNALMVFR AAKVFSQLNL PEMILNGEQL FLKPEHVIPH RQHRLLLTPN 490 500 510 520 530 540 LGGSFLSSWQ PPITDTSLKV KSHVFSLEGQ DCQYMQMFGP EARNLVLRLA QMISQAKQTA 550 560 570 580 590 600 KSISNHSPDS SANQSFLSWF GLGSPDFNGS YNGSDLDEAG YDTIRKTDEH LEKALDYFCQ 610 620 630 640 650 660 IFRLNPTQLG QLTANVDSSQ DDDGKNKLPD CIQSEDGVVL TSLGRYQIIN GLRKFDIEYQ 670 680 690 700 710 720 GDPELQPIRS YENAMLVRYL YRLSSVINKR FANSMGALCA RKDFLGKLCR HHLTSSSRKC 730 740 750 760 770 780 KKSPITSVSP SEPAAPHIRL RFLASYRTLA FLFIFYILGS LLSLGPLICT FLLLIGCMFY 790 AIVQTLLSEE QKPHNN

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Kidney and Ovary.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC084096 mRNA. Translation: AAH84096.1. Different initiation. BC097653 mRNA. Translation: AAH97653.1.
RefSeq	NP_001088184.1.
UniGene	Xl.4713
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	495009.
KEGG	xla:495009.
Organism-specific databases
CTD	495009.
Xenbase	XB-FEAT-962466. smpd4.
Phylogenomic databases
HOVERGEN	Q5XHG1.
Enzyme and pathway databases
BRENDA	3.1.4.12. 648.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

NSMA3_XENLA

Accession

Primary (citable) accession number: Q5XHG1
Secondary accession number(s): Q4V7Y8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	January 23, 2007
Last modified:	October 13, 2009
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project