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Q5XGZ2

- UP44B_XENLA

UniProt

Q5XGZ2 - UP44B_XENLA

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Protein

Ubiquitin carboxyl-terminal hydrolase 44-B

Gene
usp44-b
Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Deubiquitinase that plays a key role in the spindle checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of cdc20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C) By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei283 – 2831Nucleophile By similarity
Active sitei625 – 6251Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri24 – 8562UBP-typeAdd
BLAST

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: UniProtKB
  2. ubiquitin thiolesterase activity Source: UniProtKB
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. anaphase Source: UniProtKB
  2. mitotic nuclear division Source: UniProtKB-KW
  3. negative regulation of mitotic anaphase-promoting complex activity Source: UniProtKB
  4. protein deubiquitination Source: UniProtKB
  5. regulation of spindle checkpoint Source: UniProtKB
  6. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 44-B (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 44-B
Ubiquitin thioesterase 44-B
Ubiquitin-specific-processing protease 44-B
Gene namesi
Name:usp44-b
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6254317. usp44.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 690690Ubiquitin carboxyl-terminal hydrolase 44-BPRO_0000395814Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ5XGZ2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini274 – 667394USPAdd
BLAST

Sequence similaritiesi

Contains 1 USP domain.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri24 – 8562UBP-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOVERGENiHBG018027.
KOiK11834.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5XGZ2-1 [UniParc]FASTAAdd to Basket

« Hide

MDKCKHVGRL RLAQDHSILN PQKWHCVDCN TTESVWACLS CSHVACGRYI    50
EEHALRHFQD SKHPLALEVN ELYVFCYLCD DYVLNDNTTG DLKLLRSTLS 100
AIKSQNYDCT TRSGRTLRSM VSADDSFISH EGAQAFLQNE DRAFTALWHR 150
RHALLGKVFR SWFALTPKGK QRLEEERLRE EAEHKREEAR KRRQQLKHKL 200
KEEMESTPPR KSSRLQQQIQ PSPKIESSSV QKMNQKNAPS TKQNPPAPTS 250
DKARLKKIGN SPIKRKPTVT PGVTGLRNLG NTCYMNSILQ ILSHLHVFRE 300
CFLQLDLNQT QELLAADGSG KTRLSSKYPP GAELPRVTQK HTKGQRSLAR 350
RPSFSLGLSG GASNSRNMEL IQPKEPSSKH ISLCHELHTL FQVMWSGKWA 400
LVSPFAMLHS VWRLIPAFHG YAQQDAQEFL CELLDKVQQE LETTGTRYPA 450
LIPTSQRKLI RQVLNVVNNI FHGQLLSQVT CLVCDHKSNT IEPFWDLSLE 500
FPERYHFSGK ATASQRPCLL TEMLAKFTET EALEGKIYAC DQCNKAQKQL 550
MVCRLPQVLR LHLKRFRWSG RNHREKIGVH VRFDQMLNME PYCCRESTAA 600
LRADCFIYDL SSVVMHHGKG FGSGHYTAFC YNPEGGFWVH CNDSKLHSCA 650
VEEVCKAQAY ILFYTQRVTQ ENGHLSERLP LHDSPQSPPP 690
Length:690
Mass (Da):78,910
Last modified:November 23, 2004 - v1
Checksum:i878E83A38095E40B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC084285 mRNA. Translation: AAH84285.1.
RefSeqiNP_001088277.1. NM_001094808.1.
UniGeneiXl.49566.

Genome annotation databases

GeneIDi495110.
KEGGixla:495110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC084285 mRNA. Translation: AAH84285.1 .
RefSeqi NP_001088277.1. NM_001094808.1.
UniGenei Xl.49566.

3D structure databases

ProteinModelPortali Q5XGZ2.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 495110.
KEGGi xla:495110.

Organism-specific databases

CTDi 495110.
Xenbasei XB-GENE-6254317. usp44.

Phylogenomic databases

HOVERGENi HBG018027.
KOi K11834.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
SMARTi SM00290. ZnF_UBP. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiUP44B_XENLA
AccessioniPrimary (citable) accession number: Q5XGZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: November 23, 2004
Last modified: September 3, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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