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Protein

Ubiquitin carboxyl-terminal hydrolase 44-B

Gene

usp44-b

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Deubiquitinase that plays a key role in the spindle checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of cdc20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C) (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei283 – 2831NucleophilePROSITE-ProRule annotation
Active sitei625 – 6251Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri24 – 8562UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: UniProtKB
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. mitotic nuclear division Source: UniProtKB-KW
  3. negative regulation of APC-Cdc20 complex activity Source: UniProtKB
  4. protein deubiquitination Source: UniProtKB
  5. regulation of spindle checkpoint Source: UniProtKB
  6. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 44-B (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 44-B
Ubiquitin thioesterase 44-B
Ubiquitin-specific-processing protease 44-B
Gene namesi
Name:usp44-b
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6254317. usp44.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 690690Ubiquitin carboxyl-terminal hydrolase 44-BPRO_0000395814Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ5XGZ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini274 – 667394USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family. USP44 subfamily.Curated
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri24 – 8562UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOVERGENiHBG018027.
KOiK11834.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5XGZ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKCKHVGRL RLAQDHSILN PQKWHCVDCN TTESVWACLS CSHVACGRYI
60 70 80 90 100
EEHALRHFQD SKHPLALEVN ELYVFCYLCD DYVLNDNTTG DLKLLRSTLS
110 120 130 140 150
AIKSQNYDCT TRSGRTLRSM VSADDSFISH EGAQAFLQNE DRAFTALWHR
160 170 180 190 200
RHALLGKVFR SWFALTPKGK QRLEEERLRE EAEHKREEAR KRRQQLKHKL
210 220 230 240 250
KEEMESTPPR KSSRLQQQIQ PSPKIESSSV QKMNQKNAPS TKQNPPAPTS
260 270 280 290 300
DKARLKKIGN SPIKRKPTVT PGVTGLRNLG NTCYMNSILQ ILSHLHVFRE
310 320 330 340 350
CFLQLDLNQT QELLAADGSG KTRLSSKYPP GAELPRVTQK HTKGQRSLAR
360 370 380 390 400
RPSFSLGLSG GASNSRNMEL IQPKEPSSKH ISLCHELHTL FQVMWSGKWA
410 420 430 440 450
LVSPFAMLHS VWRLIPAFHG YAQQDAQEFL CELLDKVQQE LETTGTRYPA
460 470 480 490 500
LIPTSQRKLI RQVLNVVNNI FHGQLLSQVT CLVCDHKSNT IEPFWDLSLE
510 520 530 540 550
FPERYHFSGK ATASQRPCLL TEMLAKFTET EALEGKIYAC DQCNKAQKQL
560 570 580 590 600
MVCRLPQVLR LHLKRFRWSG RNHREKIGVH VRFDQMLNME PYCCRESTAA
610 620 630 640 650
LRADCFIYDL SSVVMHHGKG FGSGHYTAFC YNPEGGFWVH CNDSKLHSCA
660 670 680 690
VEEVCKAQAY ILFYTQRVTQ ENGHLSERLP LHDSPQSPPP
Length:690
Mass (Da):78,910
Last modified:November 22, 2004 - v1
Checksum:i878E83A38095E40B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC084285 mRNA. Translation: AAH84285.1.
RefSeqiNP_001088277.1. NM_001094808.1.
UniGeneiXl.49566.

Genome annotation databases

GeneIDi495110.
KEGGixla:495110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC084285 mRNA. Translation: AAH84285.1.
RefSeqiNP_001088277.1. NM_001094808.1.
UniGeneiXl.49566.

3D structure databases

ProteinModelPortaliQ5XGZ2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi495110.
KEGGixla:495110.

Organism-specific databases

CTDi495110.
XenbaseiXB-GENE-6254317. usp44.

Phylogenomic databases

HOVERGENiHBG018027.
KOiK11834.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiUP44B_XENLA
AccessioniPrimary (citable) accession number: Q5XGZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 12, 2010
Last sequence update: November 22, 2004
Last modified: March 3, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.