Reviewed, UniProtKB/Swiss-Prot Q5XGM3 (BHMT1_XENLA)

Names and origin

Protein names

Recommended name:
    Betaine--homocysteine S-methyltransferase 1
    EC=2.1.1.5

Gene names

Name:

bhmt

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline By similarity.
Catalytic activity	Trimethylammonioacetate + L-homocysteine = dimethylglycine + L-methionine.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 Hcy-binding domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	betaine-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	Betaine--homocysteine S-methyltransferase 1		PRO_0000273222
Regions
	Domain	8 – 311	304	Hcy-binding
Sites
	Metal binding	214	1	Zinc By similarity
	Metal binding	296	1	Zinc By similarity
	Metal binding	297	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5XGM3-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: D7FAFA7DD70F3782	FASTA	403	44,181
	10 20 30 40 50 60 MAPVGAKKGL LERLDAGEVV IGDGGFVFAL EKRGYVKAGP WTPEAAVEHP EAVRQLHREF 70 80 90 100 110 120 LRAGANVMQT FTFYASDDKL ENRGNYVAEK ISGQKVNEVA CDIAREVANE GDALVAGGVS 130 140 150 160 170 180 QTPSYLSCKS EVEVKGIFRK QLDVFIKKNV DFLIAEYFEH VEEAVWAVEV LKESGKPVAA 190 200 210 220 230 240 TLCIGPEGDL NGVSPGECAV RLAKAGASVV GVNCHFDPMT CVATVKLMKE GLVAAKVKAH 250 260 270 280 290 300 LMTQPLAYHT PDCGKQGFID LPEFPFALEP RIVTRWDIHK YARAAYDLGV RYIGGCCGFE 310 320 330 340 350 360 PYHTRAIAEE LAPERGFLPK GSEKHGSWGS GLEMHTKPWV RARARRDYWE KLPPASGRPY 370 380 390 400 CPSMSKPDEW GVTKGDADLM QQKEATTEQQ LKDLIAKQGI KSN

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC084414 mRNA. Translation: AAH84414.1.
RefSeq	NP_001088416.1.
UniGene	Xl.21139
3D structure databases
HSSP	HSSP built from PDB template 1LT7 based on UniProtKB Q93088.
SMR	Q5XGM3. Positions 7-394.
ModBase	Search...
Genome annotation databases
GeneID	495275.
KEGG	xla:495275.
Organism-specific databases
CTD	495275.
Xenbase	XB-FEAT-1008381. bhmt.
Phylogenomic databases
HOVERGEN	Q5XGM3.
Enzyme and pathway databases
BRENDA	2.1.1.5. 648.
Family and domain databases
InterPro	IPR017226. Betaine-hCys_S-MeTrfase_BHMT. IPR003726. S_MeTrfase. [Graphical view]
Gene3D	G3DSA:3.20.20.330. S_methyl_trans. 1 hit.
Pfam	PF02574. S-methyl_trans. 1 hit. [Graphical view]
PIRSF	PIRSF037505. Betaine_HMT. 1 hit.
PROSITE	PS50970. HCY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BHMT1_XENLA

Accession

Primary (citable) accession number: Q5XGM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	November 23, 2004
Last modified:	November 24, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project