Q5XGB9 (AMPL_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase 3
Short name=LAP-3
Leucyl aminopeptidase
Proline aminopeptidase
EC=3.4.11.5
Prolyl aminopeptidase

Gene names

Name:

lap3

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. Release of N-terminal proline from a peptide.
Cofactor	Binds 2 zinc ions per subunit. One zinc ion is tightly bound and essential for enzyme activity, while the second metal coordination site can be occupied by zinc, magnesium or manganese to give enzymes of different activities By similarity.
Subunit structure	Homohexamer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding Zinc
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 520	520	Cytosol aminopeptidase		PRO_0000274147
Sites
	Active site	292	1	By similarity
	Active site	366	1	By similarity
	Metal binding	280	1	Zinc 2 By similarity
	Metal binding	285	1	Zinc 1 By similarity
	Metal binding	285	1	Zinc 2 By similarity
	Metal binding	303	1	Zinc 2 By similarity
	Metal binding	362	1	Zinc 1 By similarity
	Metal binding	364	1	Zinc 1 By similarity
	Metal binding	364	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5XGB9 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 33ADD5840F6E7837	FASTA	520	56,333
	10 20 30 40 50 60 MLPFRTLLKW SVNRNCCRGF AVSQQNYNSV KKGLVLGVYE KEKEEESLTL TNAGDAVDNA 70 80 90 100 110 120 VLGKLRDQLA RSGPSLKKGK SRIFYGLHED FPSIVVVGLG KKSAGVNQHE LWNEAKENIR 130 140 150 160 170 180 AAVSVGCRQM QDMEIVQVEV DPCGDAQAAA EGAVLGLFEY NEMKKKKKKA VTTHLHGSSE 190 200 210 220 230 240 ITAWEKGVLY AEGQNLARHL MEAPANYITP TKFAETFEQR LANMGSNVKV FTRSKQWIEE 250 260 270 280 290 300 QQMGAFLSVA KGSEEPPVFL EIHYSGSSDA SQPPLVFVGK GVTFDSGGIS LKPSSGMDAM 310 320 330 340 350 360 RGDMGGAATV CSAITTAAKL KLPINIISLA PLCENMPNGR ANKPGDVVKA KNGKTIQVDN 370 380 390 400 410 420 TDAEGRLLLA DALCYAHSFN PRAIVNAATL TGAMDVALGS AAAGVFTNSS WLWTHLQEAS 430 440 450 460 470 480 VVTGDRVWRM PLFEHYSKQV TESALADLNN IGKYSRSGGA CTAAAFLKEF VTAPHWAHLD 490 500 510 520 IAGVMSNKDE VPYLRKGMSG RPTRTLIEFA ARLSEDKQTI

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC084523 mRNA. Translation: AAH84523.1.
RefSeq	NP_001011124.1. NM_001011124.1.
UniGene	Str.9934.
3D structure databases
HSSP	HSSP built from PDB template 2EWB based on UniProtKB P00727.
ProteinModelPortal	Q5XGB9.
SMR	Q5XGB9. Positions 32-516.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5XGB9.
Protein family/group databases
MEROPS	M17.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	496537.
KEGG	xtr:496537.
Organism-specific databases
CTD	51056.
Xenbase	XB-GENE-1006711. lap3.
Phylogenomic databases
eggNOG	veNOG10515.
GeneTree	ENSGT00530000063255.
HOGENOM	HBG742580.
HOVERGEN	HBG003320.
InParanoid	Q5XGB9.
OMA	KLFEASV.
OrthoDB	EOG4P2Q21.
Gene expression databases
Bgee	Q5XGB9.
Family and domain databases
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K11142.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPL_XENTR

Accession

Primary (citable) accession number: Q5XGB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	November 23, 2004
Last modified:	November 16, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program