Reviewed, UniProtKB/Swiss-Prot Q5XG87 (POLS_HUMAN)

Names and origin

Protein names

Recommended name:
    DNA polymerase sigma
    EC=2.7.7.7
Alternative name(s):
    Topoisomerase-related function protein 4-1
      Short name=TRF4-1
    LAK-1
    DNA polymerase kappa

Gene names

Name:	POLS
Synonyms:	TRF4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	542 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	DNA polymerase, probably involved in DNA repair. May play a role in sister chromatid cohesion. Ref.5
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the DNA polymerase type-B-like family.
Sequence caution	The sequence AAD45198.1 differs from that shown. Reason: Frameshift at position 474.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA synthesis Mitosis
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW double-strand break repair Ref.1 Non-traceable author statement. Source: UniProtKB mitotic chromosome condensation Ref.1 Non-traceable author statement. Source: UniProtKB response to drug Ref.1 Inferred from direct assay. Source: UniProtKB sister chromatid cohesion Ref.1 Traceable author statement. Source: UniProtKB
Cellular component	nucleus Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW SMC protein binding Ref.1 Traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 542	542	DNA polymerase sigma		PRO_0000120308
Regions
	Compositional bias	346 – 372	27	Ser-rich
Sites
	Active site	49	1	By similarity
Natural variations
	Natural variant	326	1	N → S: dbSNP rs28381415. Ref.3		VAR_021175
	Natural variant	396	1	G → S: dbSNP rs28381418. Ref.3		VAR_021176

Sequences

	Sequence		Length	Mass (Da)
	Q5XG87-1 [UniParc]. Last modified February 1, 2005. Version 2. Checksum: 233DA7E2222C2FB0	FASTA	542	59,874
	10 20 30 40 50 60 MSPCPEEAAM RREVVKRIET VVKDLWPTAD VQIFGSFSTG LYLPTSDIDL VVFGKWERPP 70 80 90 100 110 120 LQLLEQALRK HNVAEPCSIK VLDKATVPII KLTDQETEVK VDISFNMETG VRAAEFIKNY 130 140 150 160 170 180 MKKYSLLPYL ILVLKQFLLQ RDLNEVFTGG ISSYSLILMA ISFLQLHPRI DARRADENLG 190 200 210 220 230 240 MLLVEFFELY GRNFNYLKTG IRIKEGGAYI AKEEIMKAMT SGYRPSMLCI EDPLLPGNDV 250 260 270 280 290 300 GRSSYGAMQV KQVFDYAYIV LSHAVSPLAR SYPNRDAEST LGRIIKVTQE VIDYRRWIKE 310 320 330 340 350 360 KWGSKAHPSP GMDSRIKIKE RIATCNGEQT QNREPESPYG QRLTLSLSSP QLLSSGSSAS 370 380 390 400 410 420 SVSSLSGSDV DSDTPPCTTP SVYQFSLQAP APLMAGLPTA LPMPSGKPQP TTSRTLIMTT 430 440 450 460 470 480 NNQTRFTIPP PTLGVAPVPC RQAGVEGTAS LKAVHHMSSP AIPSASPNPL SSPHLYHKQH 490 500 510 520 530 540 NGMKLSMKGS HGHTQGGGYS SVGSGGVRPP VGNRGHHQYN RTGWRRKKHT HTRDSLPVSL SR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The topoisomerase-related function gene TRF4 affects cellular sensitivity to the antitumor agent camptothecin." Walowsky C., Fitzhugh D.J., Castano I.B., Ju J.Y., Levin N.A., Christman M.F. J. Biol. Chem. 274:7302-7308(1999) [PubMed: 10066793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"mLT positive LAK-cell clone No. 1." Abe Y., Takaoka Y. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	NIEHS SNPs program Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS SER-326 AND SER-396.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cerebellum and Lung.
[5]	"Pol kappa: a DNA polymerase required for sister chromatid cohesion." Wang Z., Castano I.B., De Las Penas A., Adams C., Christman M.F. Science 289:774-779(2000) [PubMed: 10926539] [Abstract] Cited for: FUNCTION.
[6]	"Evidence that replication fork components catalyze establishment of cohesion between sister chromatids." Carson D.R., Christman M.F. Proc. Natl. Acad. Sci. U.S.A. 98:8270-8275(2001) [PubMed: 11459963] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF089896 mRNA. Translation: AAD45198.1. Sequence problems. AB005754 mRNA. Translation: BAA24434.2. AY623114 Genomic DNA. Translation: AAT38110.1. BC084567 mRNA. Translation: AAH84567.2. BC117137 mRNA. Translation: AAI17138.1. BC126106 mRNA. Translation: AAI26107.1.
IPI	IPI00418630.
RefSeq	NP_008930.1.
UniGene	Hs.481542
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q5XG87.
Proteomic databases
PRIDE	Q5XG87.
Genome annotation databases
Ensembl	ENSG00000112941. Homo sapiens. [Contig view]
GeneID	11044.
KEGG	hsa:11044.
Organism-specific databases
GeneCards	GC05P006790.
H-InvDB	HIX0031938.
HGNC	HGNC:16705. POLS.
MIM	605198. gene.
PharmGKB	PA33523.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q5XG87.
HOVERGEN	Q5XG87.
OMA	Q5XG87. HLYHKQH.
Enzyme and pathway databases
BRENDA	2.7.7.7. 247.
Gene expression databases
ArrayExpress	Q5XG87.
Bgee	Q5XG87.
CleanEx	HS_POLS.
GermOnline	ENSG00000112941. Homo sapiens.
Family and domain databases
InterPro	IPR002934. Nucleotidyltransferase. IPR002058. PAP_assoc. [Graphical view]
Pfam	PF01909. NTP_transf_2. 1 hit. PF03828. PAP_assoc. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	41972.
SOURCE	Search...

Entry information

Entry name

POLS_HUMAN

Accession

Primary (citable) accession number: Q5XG87
Secondary accession number(s): O43289, Q17RZ1, Q9Y6C1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	February 1, 2005
Last modified:	June 16, 2009
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)