Reviewed, UniProtKB/Swiss-Prot Q5XF03 (GPAT8_ARATH)

Names and origin

Protein names

Recommended name:
    Probable glycerol-3-phosphate acyltransferase 8
    EC=2.3.1.15

Gene names

Name:	GPAT8
Ordered Locus Names:	At4g00400/At4g00410
ORF Names:	F5I10.4/F5I10.5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis By similarity.
Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.
Sequence caution	The sequence AAB62826.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At4g00400 and At4g00410. The sequence AAB62827.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At4g00400 and At4g00410. The sequence AAF02784.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At4g00400 and At4g00410. The sequence AAF02785.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At4g00400 and At4g00410. The sequence CAB80798.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At4g00400 and At4g00410. The sequence CAB80799.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At4g00400 and At4g00410.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cutin biosynthetic process Inferred from mutant phenotype. Source: TAIR phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Non-traceable author statement. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Probable glycerol-3-phosphate acyltransferase 8		PRO_0000195256
Regions
	Transmembrane	42 – 62	21	Potential
	Transmembrane	64 – 84	21	Potential
	Transmembrane	243 – 263	21	Potential
	Motif	310 – 315	6	HXXXXD motif

Sequences

	Sequence		Length	Mass (Da)
	Q5XF03-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: A1AD4675F0FC3F0D	FASTA	500	55,867
	10 20 30 40 50 60 MSPEKKSQNF PPITECRDGE YDSIAADLDG TLLLSRSSFP YFMLVAVEAG SLLRGLILLL 70 80 90 100 110 120 SLPFVIISYL FVSESLGIQI LIFISFAGLK IRDIELVSRA VLPRFYAADV RKDSFEVFDK 130 140 150 160 170 180 CKRKVVVTAN PIVMVEAFVK DYLGGDKVLG TEIEVNPKTN RATGFVKKPG VLVGDLKRLA 190 200 210 220 230 240 ILKEFGNESP DLGLGDRTSD HDFMSLCKKG YMVHATKSAT TIPKERLKNR IVFHDGRLAQ 250 260 270 280 290 300 RPTPLNAIIT YLWLPFGFIL SIIRVYFNLP LPERFVRYTY EMLGIHLTIR GHRPPPPSPG 310 320 330 340 350 360 TLGNLYVLNH RTALDPIIVA IALGRKICCV TYSVSRLSLM LSPIPAVALT RDRATDAANM 370 380 390 400 410 420 RKLLEKGDLV ICPEGTTCRE EYLLRFSALF AELSDRIVPV AMNCKQGMFN GTTVRGVKFW 430 440 450 460 470 480 DPYFFFMNPR PSYEATFLDR LPEEMTVNGG GKTPIEVANY VQKVIGAVLG FECTELTRKD 490 500 KYLLLGGNDG KVESINNTKK

References

[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Arabidopsis ORF clones." Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF013293 Genomic DNA. Translation: AAB62826.1. Sequence problems. AF013293 Genomic DNA. Translation: AAB62827.1. Sequence problems. AL161471 Genomic DNA. Translation: CAB80798.1. Sequence problems. AL161471 Genomic DNA. Translation: CAB80799.1. Sequence problems. AF195115 Genomic DNA. Translation: AAF02784.1. Sequence problems. AF195115 Genomic DNA. Translation: AAF02785.1. Sequence problems. BT015813 mRNA. Translation: AAU94376.1.
IPI	IPI00530223.
PIR	T01530. T01531.
RefSeq	NP_191950.2.
UniGene	At.34564
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q5XF03.
Genome annotation databases
GeneID	828140.
GenomeReviews	Gene locus AT4G00400 in contig CT486007_GR.
KEGG	ath:AT4G00400.
NMPDR	fig|3702.1.peg.17804.
Organism-specific databases
TAIR	At4g00400.
Phylogenomic databases
HOGENOM	HBG590278.
InParanoid	Q5XF03.
OMA	QRVIAKE.
Enzyme and pathway databases
BRENDA	2.3.1.15. 302.
Gene expression databases
Genevestigator	Q5XF03.
Family and domain databases
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPAT8_ARATH

Accession

Primary (citable) accession number: Q5XF03
Secondary accession number(s): O23061, O23062

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	November 23, 2004
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)