Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-ketoacyl-CoA synthase 2

Gene

KCS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Mediates the synthesis of VLCFAs from 22 to 26 carbons in length (e.g. C22, C24, C26) (PubMed:15277688). Involved in the elongation of C20 fatty acid suberin precursors (PubMed:18786002). Functionally redundant with KCS20 in the two-carbon elongation of C22 fatty acids that is required for cuticular wax and root suberin biosynthesis (PubMed:19619160).3 Publications

Catalytic activityi

A very-long-chain acyl-CoA + malonyl-CoA = CoA + a very-long-chain 3-oxoacyl-CoA + CO2.Curated

Enzyme regulationi

Inhibited by K3 herbicides such as allidochlor, anilofos, cafenstrole and flufenacet (PubMed:15277688). Strongly inhibited by metazachlor (PubMed:22284369).2 Publications

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei241 – 2411By similarity
Active sitei320 – 3201By similarity
Active sitei407 – 4071By similarity
Active sitei411 – 4111By similarity
Active sitei444 – 4441By similarity

GO - Molecular functioni

GO - Biological processi

  • fatty acid biosynthetic process Source: UniProtKB-UniPathway
  • response to osmotic stress Source: TAIR
  • response to wounding Source: TAIR
  • suberin biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT1G04220-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-ketoacyl-CoA synthase 21 Publication (EC:2.3.1.199Curated)
Short name:
KCS-21 Publication
Alternative name(s):
Docosanoic acid synthase1 Publication
Very long-chain fatty acid condensing enzyme 21 Publication
Short name:
VLCFA condensing enzyme 21 Publication
Gene namesi
Name:KCS21 Publication
Synonyms:DAISY1 Publication, KCS171 Publication
Ordered Locus Names:At1g04220Imported
ORF Names:F20D22.1Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G04220.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei36 – 5621HelicalSequence analysisAdd
BLAST
Transmembranei78 – 9821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype in flowers or siliques, but reduced root growth. Suberin with a significant decrease in VLCFA derivatives longer than C20 (PubMed:18786002). No visible phenotype, but reduced root growth. Kcs2 and kcs20 double mutants have a glossy green appearance due to a significant reduction of the amount of epicuticular wax crystals on the stems and siliques, a significant reduction of C22 and C24 VLCFA derivatives in aliphatic suberin and a roots growth retardation and abnormal lamellation of the suberin layer in the endodermis (PubMed:19619160).2 Publications

Chemistry

ChEMBLiCHEMBL2242736.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5285283-ketoacyl-CoA synthase 2PRO_0000249109Add
BLAST

Proteomic databases

PaxDbiQ5XEP9.
PRIDEiQ5XEP9.

Expressioni

Tissue specificityi

Expressed in siliques, flowers and stems (PubMed:18465198). In young seedlings, expressed in the central cylinder of primary roots, in emerging lateral roots and in their root cap, but not in aboveground tissues such as hypocotyls, cotyledons and leaves. Expressed in sepals in mature flowers and in the chalaza and micropyle region of developing seeds shortly prior to or just after the detachment from the funiculus (PubMed:18786002). Expressed in roots, flowers, cauline leaves and siliques (PubMed:19619160).3 Publications

Inductioni

Repressed by herbicides such as flufenacet and benfuresate (PubMed:12916765). Up-regulated by wounding, drought and salt (PubMed:18786002). Strongly up-regulated by abscisic acid and drought, and to a lower level, by salt and osmotic stress (PubMed:19619160). Up-regulated by the MYB94 transcription factor (PubMed:25305760).4 Publications

Gene expression databases

GenevisibleiQ5XEP9. AT.

Interactioni

Protein-protein interaction databases

BioGridi24813. 2 interactions.
MINTiMINT-8063327.
STRINGi3702.AT1G04220.1.

Structurei

3D structure databases

ProteinModelPortaliQ5XEP9.
SMRiQ5XEP9. Positions 150-486.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini97 – 388292FAESequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated
Contains 1 FAE (fatty acid elongase) domain.Sequence analysis

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHMW. Eukaryota.
ENOG410Y5VH. LUCA.
HOGENOMiHOG000238893.
InParanoidiQ5XEP9.
KOiK15397.
OMAiFTTRPRK.
OrthoDBiEOG093606UZ.
PhylomeDBiQ5XEP9.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012392. 3-ktacl-CoA_syn.
IPR013747. ACP_syn_III_C.
IPR013601. FAE1_typ3_polyketide_synth.
IPR016039. Thiolase-like.
[Graphical view]
PANTHERiPTHR31561. PTHR31561. 1 hit.
PfamiPF08541. ACP_syn_III_C. 1 hit.
PF08392. FAE1_CUT1_RppA. 1 hit.
[Graphical view]
PIRSFiPIRSF036417. 3-ktacl-CoA_syn. 1 hit.
SUPFAMiSSF53901. SSF53901. 3 hits.

Sequencei

Sequence statusi: Complete.

Q5XEP9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNENHIQSDH MNNTIHVTNK KLPNFLLSVR LKYVKLGYHY LISNAVYILI
60 70 80 90 100
LPVGLLAATS SSFSLTDLTL LYNHLLKFHF LSSTLFAALL IFLTTLYFTT
110 120 130 140 150
RPRRIFLLDF ACYKPDSSLI CTRETFMDRS QRVGIFTEDN LAFQQKILER
160 170 180 190 200
SGLGQKTYFP EALLRVPPNP CMSEARKEAE TVMFGAIDAV LEKTGVNPKD
210 220 230 240 250
IGILVVNCSL FNPTPSLSAM IVNKYKLRGN VLSYNLGGMG CSAGLISIDL
260 270 280 290 300
AKQLLQVQPN SYALVVSTEN ITLNWYLGND RSMLLSNCIF RMGGAAVLLS
310 320 330 340 350
NRSSDRCRSK YQLIHTVRTH KGSDDNAFNC VYQREDNDDN KQIGVSLSKN
360 370 380 390 400
LMAIAGEALK TNITTLGPLV LPMSEQLLFF ATLVARKVFN VKKIKPYIPD
410 420 430 440 450
FKLAFEHFCI HAGGRAVLDE IEKNLDLSEW HMEPSRMTLN RFGNTSSSSL
460 470 480 490 500
WYELAYSEAK GRIKRGDRTW QIAFGSGFKC NSAVWRALRT IDPSKEKKKK
510 520
TNPWIDEIHE FPVPVPRTSP VTSSSESR
Length:528
Mass (Da):59,528
Last modified:September 5, 2006 - v2
Checksum:iFC47749CBBB26B3E
GO

Sequence cautioni

The sequence BAD95022 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti453 – 4531E → G in AAL67132 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002411 Genomic DNA. Translation: AAC16740.1.
CP002684 Genomic DNA. Translation: AEE27671.1.
AY074518 mRNA. Translation: AAL67132.1.
BT015917 mRNA. Translation: AAU95453.1.
AK222101 mRNA. Translation: BAD95022.1. Different initiation.
PIRiT00951.
RefSeqiNP_171918.1. NM_100303.3.
UniGeneiAt.20865.
At.48152.

Genome annotation databases

EnsemblPlantsiAT1G04220.1; AT1G04220.1; AT1G04220.
GeneIDi839578.
GrameneiAT1G04220.1; AT1G04220.1; AT1G04220.
KEGGiath:AT1G04220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002411 Genomic DNA. Translation: AAC16740.1.
CP002684 Genomic DNA. Translation: AEE27671.1.
AY074518 mRNA. Translation: AAL67132.1.
BT015917 mRNA. Translation: AAU95453.1.
AK222101 mRNA. Translation: BAD95022.1. Different initiation.
PIRiT00951.
RefSeqiNP_171918.1. NM_100303.3.
UniGeneiAt.20865.
At.48152.

3D structure databases

ProteinModelPortaliQ5XEP9.
SMRiQ5XEP9. Positions 150-486.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24813. 2 interactions.
MINTiMINT-8063327.
STRINGi3702.AT1G04220.1.

Chemistry

ChEMBLiCHEMBL2242736.

Proteomic databases

PaxDbiQ5XEP9.
PRIDEiQ5XEP9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G04220.1; AT1G04220.1; AT1G04220.
GeneIDi839578.
GrameneiAT1G04220.1; AT1G04220.1; AT1G04220.
KEGGiath:AT1G04220.

Organism-specific databases

TAIRiAT1G04220.

Phylogenomic databases

eggNOGiENOG410IHMW. Eukaryota.
ENOG410Y5VH. LUCA.
HOGENOMiHOG000238893.
InParanoidiQ5XEP9.
KOiK15397.
OMAiFTTRPRK.
OrthoDBiEOG093606UZ.
PhylomeDBiQ5XEP9.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciARA:AT1G04220-MONOMER.

Miscellaneous databases

PROiQ5XEP9.

Gene expression databases

GenevisibleiQ5XEP9. AT.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012392. 3-ktacl-CoA_syn.
IPR013747. ACP_syn_III_C.
IPR013601. FAE1_typ3_polyketide_synth.
IPR016039. Thiolase-like.
[Graphical view]
PANTHERiPTHR31561. PTHR31561. 1 hit.
PfamiPF08541. ACP_syn_III_C. 1 hit.
PF08392. FAE1_CUT1_RppA. 1 hit.
[Graphical view]
PIRSFiPIRSF036417. 3-ktacl-CoA_syn. 1 hit.
SUPFAMiSSF53901. SSF53901. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiKCS2_ARATH
AccessioniPrimary (citable) accession number: Q5XEP9
Secondary accession number(s): O64485, Q56WE1, Q8VXW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: September 5, 2006
Last modified: September 7, 2016
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-11 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.