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Protein

Phosphoribosylamine--glycine ligase

Gene

purD

Organism
Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi284Magnesium or manganeseUniRule annotation1
Metal bindingi286Magnesium or manganeseUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi134 – 195ATPUniRule annotationAdd BLAST62

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPYO286636:G1G3V-109-MONOMER
UniPathwayiUPA00074; UER00125

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylamine--glycine ligaseUniRule annotation (EC:6.3.4.13UniRule annotation)
Alternative name(s):
GARSUniRule annotation
Glycinamide ribonucleotide synthetaseUniRule annotation
Phosphoribosylglycinamide synthetaseUniRule annotation
Gene namesi
Name:purDUniRule annotation
Ordered Locus Names:M6_Spy0078
OrganismiStreptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Taxonomic identifieri286636 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000001167 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001514911 – 421Phosphoribosylamine--glycine ligaseAdd BLAST421

Proteomic databases

PRIDEiQ5XEF0

Structurei

3D structure databases

ProteinModelPortaliQ5XEF0
SMRiQ5XEF0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini108 – 314ATP-graspUniRule annotationAdd BLAST207

Sequence similaritiesi

Belongs to the GARS family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000033463
KOiK01945

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
3.90.600.10, 1 hit
HAMAPiMF_00138 GARS, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR016185 PreATP-grasp_dom_sf
IPR020561 PRibGlycinamid_synth_ATP-grasp
IPR000115 PRibGlycinamide_synth
IPR020560 PRibGlycinamide_synth_C-dom
IPR037123 PRibGlycinamide_synth_C_sf
IPR020559 PRibGlycinamide_synth_CS
IPR020562 PRibGlycinamide_synth_N
IPR011054 Rudment_hybrid_motif
PfamiView protein in Pfam
PF01071 GARS_A, 1 hit
PF02843 GARS_C, 1 hit
PF02844 GARS_N, 1 hit
SMARTiView protein in SMART
SM01210 GARS_C, 1 hit
SUPFAMiSSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR00877 purD, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00184 GARS, 1 hit

Sequencei

Sequence statusi: Complete.

Q5XEF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLVVGSGG REHAIAKKLL ASKGVDQVFV APGNDGMTLD GLDLVNIVVS
60 70 80 90 100
EHSRLIAFAK ENEISWAFIG PDDALAAGIV DDFNSAGLRA FGPTKAAAEL
110 120 130 140 150
EWSKDFAKEI MVKYNVPTAA YGTFSDFEKA KAYIEEQGAP IVVKADGLAL
160 170 180 190 200
GKGVVVAETV EQAVEAAQEM LLDNKFGDSG ARVVIEEFLD GEEFSLFAFV
210 220 230 240 250
NGDKFYIMPT AQDHKRAFDG DKGPNTGGMG AYAPVPHLPQ SVVDTAVETI
260 270 280 290 300
VRPVLEGMVA EGRPYLGVLY VGLILTADGP KVIEFNSRFG DPETQIILPR
310 320 330 340 350
LTSDFAQNID DIMMGIEPYI TWQNDGVTLG VVVASEGYPL DYEKGVPLPE
360 370 380 390 400
KTDGDIITYY AGAKFAENSE LLLSNGGRVY MLVTTEDSVK AGQDKIYTQL
410 420
AQQDTTGLFY RNDIGNKAIK E
Length:421
Mass (Da):45,385
Last modified:June 21, 2005 - v2
Checksum:i7E6478797C0AC8EB
GO

Sequence cautioni

The sequence AAT86213 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000003 Genomic DNA Translation: AAT86213.1 Different initiation.
RefSeqiWP_021340276.1, NC_006086.1

Genome annotation databases

EnsemblBacteriaiAAT86213; AAT86213; M6_Spy0078
KEGGispa:M6_Spy0078

Similar proteinsi

Entry informationi

Entry nameiPUR2_STRP6
AccessioniPrimary (citable) accession number: Q5XEF0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: May 23, 2018
This is version 98 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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