Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q5XEF0 (PUR2_STRP6) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:M6_Spy0078
OrganismStreptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394) [Complete proteome] [HAMAP]
Taxonomic identifier286636 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length421 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence caution

The sequence AAT86213.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 421421Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151491

Regions

Domain108 – 314207ATP-grasp
Nucleotide binding134 – 19562ATP By similarity

Sites

Metal binding2841Magnesium or manganese By similarity
Metal binding2861Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5XEF0 [UniParc].

Last modified June 21, 2005. Version 2.
Checksum: 7E6478797C0AC8EB

FASTA42145,385
        10         20         30         40         50         60 
MKLLVVGSGG REHAIAKKLL ASKGVDQVFV APGNDGMTLD GLDLVNIVVS EHSRLIAFAK 

        70         80         90        100        110        120 
ENEISWAFIG PDDALAAGIV DDFNSAGLRA FGPTKAAAEL EWSKDFAKEI MVKYNVPTAA 

       130        140        150        160        170        180 
YGTFSDFEKA KAYIEEQGAP IVVKADGLAL GKGVVVAETV EQAVEAAQEM LLDNKFGDSG 

       190        200        210        220        230        240 
ARVVIEEFLD GEEFSLFAFV NGDKFYIMPT AQDHKRAFDG DKGPNTGGMG AYAPVPHLPQ 

       250        260        270        280        290        300 
SVVDTAVETI VRPVLEGMVA EGRPYLGVLY VGLILTADGP KVIEFNSRFG DPETQIILPR 

       310        320        330        340        350        360 
LTSDFAQNID DIMMGIEPYI TWQNDGVTLG VVVASEGYPL DYEKGVPLPE KTDGDIITYY 

       370        380        390        400        410        420 
AGAKFAENSE LLLSNGGRVY MLVTTEDSVK AGQDKIYTQL AQQDTTGLFY RNDIGNKAIK 


E 

« Hide

References

[1]"Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain."
Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E., Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M.
J. Infect. Dis. 190:727-738(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-946 / MGAS10394.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000003 Genomic DNA. Translation: AAT86213.1. Different initiation.
RefSeqYP_059396.1. NC_006086.1.

3D structure databases

ProteinModelPortalQ5XEF0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING286636.M6_Spy0078.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT86213; AAT86213; M6_Spy0078.
GeneID2942058.
KEGGspa:M6_Spy0078.
PATRIC19722216. VBIStrPyo30273_0080.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMACIAGLKN.
OrthoDBEOG69SKD1.

Enzyme and pathway databases

BioCycSPYO286636:GHNO-112-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_STRP6
AccessionPrimary (citable) accession number: Q5XEF0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: May 14, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways