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Protein

DNA repair protein RadA

Gene

radA

Organism
Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri10 – 2718C4-typeUniRule annotationAdd
BLAST
Nucleotide bindingi95 – 1028ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA repair, Stress response

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSPYO286636:GHNO-281-MONOMER.

Protein family/group databases

MEROPSiS16.A04.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RadAUniRule annotation (EC:3.6.4.-UniRule annotation)
Alternative name(s):
Branch migration protein RadAUniRule annotation
Gene namesi
Name:radAUniRule annotation
Ordered Locus Names:M6_Spy0231
OrganismiStreptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Taxonomic identifieri286636 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000001167 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 453453DNA repair protein RadAPRO_0000187940Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ5XDZ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni350 – 453104Lon-protease-likeUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi251 – 2555RadA KNRFG motifUniRule annotation

Domaini

Has a putative N-terminal zinc-finger, a middle region with homology to RecA with ATPase motifs including the RadA KNRFG motif, while the C-terminus is homologous to Lon protease.UniRule annotation

Sequence similaritiesi

Belongs to the RecA family. RadA subfamily.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri10 – 2718C4-typeUniRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000218329.
KOiK04485.
OMAiEVGCFEL.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01498. RadA_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR004504. DNA_repair_RadA.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 2 hits.
TIGRFAMsiTIGR00416. sms. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5XDZ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKKATFIC QECGYQSPKY LGRCPNCSAW SSFVEEVEVK EVKNARVSLA
60 70 80 90 100
GEKSRPVKLK DVDNISYHRT QTDMSEFNRV LGGGVVPGSL ILIGGDPGIG
110 120 130 140 150
KSTLLLQVST QLANKGTVLY VSGEESAEQI KLRSERLGDI DNEFYLYAET
160 170 180 190 200
NMQAIRTEIE NIKPDFLIID SIQTIMSPDI TGVQGSVSQV REVTAELMQL
210 220 230 240 250
AKTNNIATFI VGHVTKEGTL AGPRMLEHMV DTVLYFEGER HHTFRILRAV
260 270 280 290 300
KNRFGSTNEI GIFEMQSGGL VEVLNPSQVF LEERLDGATG SAVVVTMEGS
310 320 330 340 350
RPILAEVQSL VTPTVFGNAR RTTTGLDFNR VSLIMAVLEK RCGLLLQNQD
360 370 380 390 400
AYLKSAGGVK LDEPAIDLAV AVAIASSYKE KPTSPQEAFL GEIGLTGEIR
410 420 430 440 450
RVTRIEQRIN EAAKLGFTKV YAPKNALQGI DIPQGIEVVG VTTVGQVLKA

VFS
Length:453
Mass (Da):49,387
Last modified:November 23, 2004 - v1
Checksum:i5A04C8589F70F54C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000003 Genomic DNA. Translation: AAT86366.1.
RefSeqiWP_002986109.1. NC_006086.1.

Genome annotation databases

EnsemblBacteriaiAAT86366; AAT86366; M6_Spy0231.
KEGGispa:M6_Spy0231.
PATRICi19722561. VBIStrPyo30273_0236.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000003 Genomic DNA. Translation: AAT86366.1.
RefSeqiWP_002986109.1. NC_006086.1.

3D structure databases

ProteinModelPortaliQ5XDZ7.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS16.A04.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT86366; AAT86366; M6_Spy0231.
KEGGispa:M6_Spy0231.
PATRICi19722561. VBIStrPyo30273_0236.

Phylogenomic databases

HOGENOMiHOG000218329.
KOiK04485.
OMAiEVGCFEL.

Enzyme and pathway databases

BioCyciSPYO286636:GHNO-281-MONOMER.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01498. RadA_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR004504. DNA_repair_RadA.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 2 hits.
TIGRFAMsiTIGR00416. sms. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRADA_STRP6
AccessioniPrimary (citable) accession number: Q5XDZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: November 23, 2004
Last modified: September 7, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.