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Protein

dITP/XTP pyrophosphatase

Gene

M6_Spy0330

Organism
Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.UniRule annotation

Catalytic activityi

XTP + H2O = XMP + diphosphate.UniRule annotation
dITP + H2O = dIMP + diphosphate.UniRule annotation
ITP + H2O = IMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei196Proton acceptorUniRule annotation1
Metal bindingi196MagnesiumUniRule annotation1
Binding sitei197Substrate; via amide nitrogenUniRule annotation1
Binding sitei303SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPYO286636:GHNO-386-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
dITP/XTP pyrophosphataseUniRule annotation (EC:3.6.1.66UniRule annotation)
Alternative name(s):
Non-canonical purine NTP pyrophosphataseUniRule annotation
Non-standard purine NTP pyrophosphataseUniRule annotation
Nucleoside-triphosphate diphosphataseUniRule annotation
Nucleoside-triphosphate pyrophosphataseUniRule annotation
Short name:
NTPaseUniRule annotation
Gene namesi
Ordered Locus Names:M6_Spy0330
OrganismiStreptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Taxonomic identifieri286636 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000001167 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001782441 – 328dITP/XTP pyrophosphataseAdd BLAST328

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ5XDP8.
SMRiQ5XDP8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 129UnknownAdd BLAST129
Regioni130 – 324NTP pyrophosphataseAdd BLAST195
Regioni134 – 139Substrate bindingUniRule annotation6
Regioni280 – 283Substrate bindingUniRule annotation4
Regioni308 – 309Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the HAM1 NTPase family.UniRule annotationCurated

Phylogenomic databases

HOGENOMiHOG000293319.
KOiK02428.

Family and domain databases

CDDicd00515. HAM1. 1 hit.
Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
InterProiView protein in InterPro
IPR020922. dITP/XTP_pyrophosphatase.
IPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiView protein in Pfam
PF01725. Ham1p_like. 1 hit.
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5XDP8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKIYEYKD ENNWFIGKMT GHNLISGWGV KHRTIKKIDD LLDGIAATLD
60 70 80 90 100
WENPKGYDVS VVRYQSPLSL ITFIIDMINQ ETQREIKVIP HAGTILLMEN
110 120 130 140 150
AKLLAVYLPE GGVSTATFFA TSEQGFGDTI LIATRNEGKT KEFRNLFGQL
160 170 180 190 200
GYRVENLNDY PELPEVAETG TTFEENARLK AETISHLTGK MVLADDSGLK
210 220 230 240 250
VDALGDLPGV WSARFSGPDA TDAKNNAKLL HELAMVFDQK KRSAQFHTTL
260 270 280 290 300
VVAAPNKDSL VVEAEWPGYI ATQPKGENGF GYDPVFIVGE TGRHAAELEA
310 320
DQKNQLSHRG QAVRKLMEVF PAWQAKQS
Length:328
Mass (Da):36,445
Last modified:April 26, 2005 - v2
Checksum:i8C05ED79DA7662B8
GO

Sequence cautioni

The sequence AAT86465 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000003 Genomic DNA. Translation: AAT86465.1. Different initiation.
RefSeqiWP_011184197.1. NC_006086.1.

Genome annotation databases

EnsemblBacteriaiAAT86465; AAT86465; M6_Spy0330.
KEGGispa:M6_Spy0330.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000003 Genomic DNA. Translation: AAT86465.1. Different initiation.
RefSeqiWP_011184197.1. NC_006086.1.

3D structure databases

ProteinModelPortaliQ5XDP8.
SMRiQ5XDP8.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT86465; AAT86465; M6_Spy0330.
KEGGispa:M6_Spy0330.

Phylogenomic databases

HOGENOMiHOG000293319.
KOiK02428.

Enzyme and pathway databases

BioCyciSPYO286636:GHNO-386-MONOMER.

Family and domain databases

CDDicd00515. HAM1. 1 hit.
Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
InterProiView protein in InterPro
IPR020922. dITP/XTP_pyrophosphatase.
IPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiView protein in Pfam
PF01725. Ham1p_like. 1 hit.
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIXTPA_STRP6
AccessioniPrimary (citable) accession number: Q5XDP8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: June 7, 2017
This is version 79 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.