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Protein

Pyrrolidone-carboxylate peptidase

Gene

pcp

Organism
Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Removes 5-oxoproline from various penultimate amino acid residues except L-proline.

Catalytic activityi

Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei78 – 781By similarity
Active sitei141 – 1411By similarity
Active sitei165 – 1651By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BioCyciSPYO286636:GHNO-500-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrrolidone-carboxylate peptidase (EC:3.4.19.3)
Alternative name(s):
5-oxoprolyl-peptidase
Pyroglutamyl-peptidase I
Short name:
PGP-I
Short name:
Pyrase
Gene namesi
Name:pcp
Ordered Locus Names:M6_Spy0444
OrganismiStreptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Taxonomic identifieri286636 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000001167 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 215215Pyrrolidone-carboxylate peptidasePRO_0000184744Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ5XDD4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C15 family.Curated

Phylogenomic databases

HOGENOMiHOG000242641.
KOiK01304.
OMAiFIHVPFI.
OrthoDBiEOG6X1124.

Family and domain databases

Gene3Di3.40.630.20. 1 hit.
HAMAPiMF_00417. Pyrrolid_peptidase.
InterProiIPR000816. Peptidase_C15.
IPR016125. Peptidase_C15-like.
IPR029762. PGP-I_bact-type.
[Graphical view]
PANTHERiPTHR23402. PTHR23402. 1 hit.
PfamiPF01470. Peptidase_C15. 1 hit.
[Graphical view]
PIRSFiPIRSF015592. Prld-crbxl_pptds. 1 hit.
PRINTSiPR00706. PYROGLUPTASE.
TIGRFAMsiTIGR00504. pyro_pdase. 1 hit.
PROSITEiPS01334. PYRASE_CYS. 1 hit.
PS01333. PYRASE_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5XDD4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKILVTGFDP FGGEAINPAL EAIKKLPATI HGAEIKCIEV PTVFQKSADV
60 70 80 90 100
LQQHIESFQP DAVLCIGQAG GRTGLTPERV AINQDDARIP DNEGNQPIDT
110 120 130 140 150
PIRADGKAAY FSTLPIKAMV AAIHQAGLPA SVSNTAGTFV CNHLMYQALY
160 170 180 190 200
LVDKYCPNAK AGFMHIPFMM EQVVDKPNTA AMNLDDITRG IEAAIFAIVD
210
FKDRSDLKRV GGATH
Length:215
Mass (Da):23,132
Last modified:November 23, 2004 - v1
Checksum:i2D9E727A09704A69
GO

Mass spectrometryi

Molecular mass is 23131.60 Da from positions 1 - 215. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65717 Genomic DNA. Translation: CAA46633.1.
CP000003 Genomic DNA. Translation: AAT86579.1.
PIRiS24717.
RefSeqiWP_002994169.1. NC_006086.1.

Genome annotation databases

EnsemblBacteriaiAAT86579; AAT86579; M6_Spy0444.
KEGGispa:M6_Spy0444.
PATRICi19723026. VBIStrPyo30273_0462.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65717 Genomic DNA. Translation: CAA46633.1.
CP000003 Genomic DNA. Translation: AAT86579.1.
PIRiS24717.
RefSeqiWP_002994169.1. NC_006086.1.

3D structure databases

ProteinModelPortaliQ5XDD4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT86579; AAT86579; M6_Spy0444.
KEGGispa:M6_Spy0444.
PATRICi19723026. VBIStrPyo30273_0462.

Phylogenomic databases

HOGENOMiHOG000242641.
KOiK01304.
OMAiFIHVPFI.
OrthoDBiEOG6X1124.

Enzyme and pathway databases

BioCyciSPYO286636:GHNO-500-MONOMER.

Family and domain databases

Gene3Di3.40.630.20. 1 hit.
HAMAPiMF_00417. Pyrrolid_peptidase.
InterProiIPR000816. Peptidase_C15.
IPR016125. Peptidase_C15-like.
IPR029762. PGP-I_bact-type.
[Graphical view]
PANTHERiPTHR23402. PTHR23402. 1 hit.
PfamiPF01470. Peptidase_C15. 1 hit.
[Graphical view]
PIRSFiPIRSF015592. Prld-crbxl_pptds. 1 hit.
PRINTSiPR00706. PYROGLUPTASE.
TIGRFAMsiTIGR00504. pyro_pdase. 1 hit.
PROSITEiPS01334. PYRASE_CYS. 1 hit.
PS01333. PYRASE_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of pcp, the structural gene encoding the pyrrolidone carboxylyl peptidase from Streptococcus pyogenes."
    Cleuziat P., Robert-Baudouy J.
    Mol. Microbiol. 6:2051-2063(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: D471 / Serotype M6.
  2. "Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain."
    Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E., Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M.
    J. Infect. Dis. 190:727-738(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-946 / MGAS10394.
  3. "Two-dimensional gel electrophoresis map of Streptococcus pyogenes proteins."
    Hogan D.A., Du P., Stevenson T.I., Whitton M., Kilby G.W., Rogers J., VanBogelen R.A.
    Submitted (MAY-2000) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 3-24; 80-103 AND 190-204, MASS SPECTROMETRY.
    Strain: JRS4 / Serotype M6.

Entry informationi

Entry nameiPCP_STRP6
AccessioniPrimary (citable) accession number: Q5XDD4
Secondary accession number(s): Q01328
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: November 23, 2004
Last modified: November 11, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.