Q5XC85 (KPRS2_STRP6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase 2
Short name=RPPK 2
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase 2
Short name=P-Rib-PP synthase 2
Short name=PRPP synthase 2

Gene names

Name:	prs2
Ordered Locus Names:	M6_Spy0843

Organism

Streptococcus pyogenes serotype M6 [Complete proteome] [HAMAP]

Taxonomic identifier

301450 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583_B
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583_B
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00583_B.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.
Mass spectrometry	Molecular mass is 35707.93 Da from positions 1 - 326. Determined by ESI. Ref.2

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Ribose-phosphate pyrophosphokinase 2 HAMAP MF_00583_B		PRO_0000141213
Regions
	Region	218 – 231	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	134	1	Magnesium Potential
	Metal binding	136	1	Magnesium Potential
	Metal binding	145	1	Magnesium Potential
	Metal binding	149	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5XC85 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: A5AAAAECE67CE1AC	FASTA	326	35,723
	10 20 30 40 50 60 MTERYADKQI KLFSLTSNLP IAEKIAKAAG IPLGKMSSRQ FSDGEIMINI EETVRGDDIY 70 80 90 100 110 120 IIQSTSFPVN DNLWELLIMI DACKRASANT VNIVLPYFGY SRQDRVAKPR EPITAKLVAN 130 140 150 160 170 180 MLTKAGIDRV VTLDLHAVQV QGFFDIPVDN LFTVPLFAER YSKLGLSGSD VVVVSPKNSG 190 200 210 220 230 240 IKRARSLAEY LDSPIAIIDY AQDDSEREQG YIIGDVSGKK AILIDDILNT GKTFAEAAKI 250 260 270 280 290 300 LERSGATDTY AVASHGLFAG GAAEVLETAP IKEIIVTDSV KTKNRVPENV TYLSASDLIA 310 320 EAIIRIHERR PLSPLFSYQP KGKNNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain." Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E., Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M. J. Infect. Dis. 190:727-738(2004) [PubMed: 15272401] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-946 / MGAS10394 / Serotype M6.
[2]	"Two-dimensional gel electrophoresis map of Streptococcus pyogenes proteins." Hogan D.A., Du P., Stevenson T.I., Whitton M., Kilby G.W., Rogers J., VanBogelen R.A. Submitted (MAY-2000) to UniProtKB Cited for: PROTEIN SEQUENCE OF 164-177; 220-232 AND 310-321, MASS SPECTROMETRY. Strain: JRS4 / Serotype M6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000003 Genomic DNA. Translation: AAT86978.1.
RefSeq	YP_060161.1. NC_006086.1.
3D structure databases
ProteinModelPortal	Q5XC85.
SMR	Q5XC85. Positions 10-317.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000038768; EBSTRP00000037341; EBSTRG00000038766.
GeneID	2942249.
GenomeReviews	Gene locus M6_Spy0843 in contig CP000003_GR.
KEGG	spa:M6_Spy0843.
PATRIC	19723854. VBIStrPyo30273_0872.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000027352.
HOGENOM	HBG519284.
OMA	CTSGLTH.
ProtClustDB	PRK02458.
Enzyme and pathway databases
BioCyc	SPYO286636:M6_SPY0843-MONOMER.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B. [Tree]
InterPro	IPR000842. PRib_PP_synth_CS. IPR000836. PRibTrfase. IPR005946. Rib-P_diPkinase. [Graphical view]
KO	K00948.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. RibP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS2_STRP6

Accession

Primary (citable) accession number: Q5XC85
Secondary accession number(s): P82570

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program