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Q5XC60

- NAOX_STRP6

UniProt

Q5XC60 - NAOX_STRP6

Protein

Probable NADH oxidase

Gene

M6_Spy0868

Organism
Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the four-electron reduction of molecular oxygen to water.By similarity

    Catalytic activityi

    NADH + acceptor = NAD+ + reduced acceptor.By similarity

    Cofactori

    Binds 1 FAD per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei11 – 111Proton acceptorBy similarity
    Active sitei44 – 441Redox-activeBy similarity
    Binding sitei44 – 441FADBy similarity
    Binding sitei171 – 1711NAD; via amide nitrogenBy similarity
    Binding sitei190 – 1901NADBy similarity
    Binding sitei199 – 1991NADBy similarity
    Binding sitei254 – 2541NAD; via amide nitrogenBy similarity
    Binding sitei292 – 2921FADBy similarity
    Binding sitei308 – 3081NAD; via carbonyl oxygenBy similarity
    Binding sitei310 – 3101FAD; via amide nitrogenBy similarity
    Binding sitei339 – 3391NAD; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi8 – 125FADBy similarity
    Nucleotide bindingi110 – 1134FADBy similarity
    Nucleotide bindingi163 – 17816NADBy similarityAdd
    BLAST
    Nucleotide bindingi282 – 29211FADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. NADH dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell redox homeostasis Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NAD

    Enzyme and pathway databases

    BioCyciSPYO286636:GHNO-928-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable NADH oxidase (EC:1.6.99.3)
    Short name:
    NOXase
    Gene namesi
    Ordered Locus Names:M6_Spy0868
    OrganismiStreptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
    Taxonomic identifieri286636 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000001167: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 456456Probable NADH oxidasePRO_0000259672Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei44 – 441Cysteine sulfenic acid (-SOH)By similarity

    Keywords - PTMi

    Oxidation

    Interactioni

    Protein-protein interaction databases

    STRINGi286636.M6_Spy0868.

    Structurei

    Secondary structure

    1
    456
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 75
    Helixi11 – 2414
    Helixi25 – 273
    Beta strandi28 – 336
    Beta strandi35 – 373
    Helixi43 – 453
    Helixi46 – 505
    Beta strandi53 – 553
    Helixi58 – 603
    Helixi65 – 706
    Beta strandi74 – 763
    Beta strandi81 – 855
    Turni86 – 894
    Beta strandi90 – 956
    Beta strandi98 – 1036
    Beta strandi105 – 1095
    Beta strandi113 – 1153
    Beta strandi134 – 1363
    Beta strandi139 – 1413
    Helixi145 – 15410
    Beta strandi162 – 1665
    Helixi170 – 18112
    Beta strandi185 – 19410
    Turni195 – 1995
    Helixi202 – 21312
    Turni214 – 2163
    Beta strandi218 – 2214
    Beta strandi225 – 2295
    Beta strandi231 – 2333
    Beta strandi236 – 2416
    Beta strandi243 – 2453
    Beta strandi247 – 2515
    Beta strandi255 – 2573
    Helixi260 – 2623
    Beta strandi287 – 2893
    Helixi291 – 2933
    Beta strandi296 – 2983
    Turni299 – 3024
    Beta strandi303 – 3053
    Helixi310 – 32415
    Beta strandi338 – 3425
    Beta strandi345 – 3517
    Helixi354 – 3596
    Beta strandi364 – 37411
    Beta strandi384 – 3929
    Turni393 – 3953
    Beta strandi397 – 40711
    Helixi412 – 42211
    Helixi426 – 4316
    Turni438 – 4403
    Helixi446 – 4527

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2BC0X-ray2.00A/B2-456[»]
    2BC1X-ray2.15A/B2-456[»]
    2BCPX-ray2.10A/B2-456[»]
    ProteinModelPortaliQ5XC60.
    SMRiQ5XC60. Positions 2-456.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Redox-active center

    Phylogenomic databases

    eggNOGiCOG0446.
    HOGENOMiHOG000276710.
    KOiK00359.
    OMAiHAYIPLA.
    OrthoDBiEOG6QVRCJ.

    Family and domain databases

    Gene3Di3.30.390.30. 1 hit.
    InterProiIPR016156. FAD/NAD-linked_Rdtase_dimer.
    IPR013027. FAD_pyr_nucl-diS_OxRdtase.
    IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
    IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
    IPR001327. Pyr_OxRdtase_NAD-bd_dom.
    [Graphical view]
    PfamiPF00070. Pyr_redox. 1 hit.
    PF07992. Pyr_redox_2. 2 hits.
    PF02852. Pyr_redox_dim. 1 hit.
    [Graphical view]
    PRINTSiPR00368. FADPNR.
    SUPFAMiSSF55424. SSF55424. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5XC60-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKIVVVGAN HAGTACIKTM LTNYGDANEI VVFDQNSNIS FLGCGMALWI    50
    GEQIAGPEGL FYSDKEELES LGAKVYMESP VQSIDYDAKT VTALVDGKNH 100
    VETYDKLIFA TGSQPILPPI KGAEIKEGSL EFEATLENLQ FVKLYQNSAD 150
    VIAKLENKDI KRVAVVGAGY IGVELAEAFQ RKGKEVVLID VVDTCLAGYY 200
    DRDLTDLMAK NMEEHGIQLA FGETVKEVAG NGKVEKIITD KNEYDVDMVI 250
    LAVGFRPNTT LGNGKIDLFR NGAFLVNKRQ ETSIPGVYAI GDCATIYDNA 300
    TRDTNYIALA SNAVRTGIVA AHNACGTDLE GIGVQGSNGI SIYGLHMVST 350
    GLTLEKAKRL GFDAAVTEYT DNQKPEFIEH GNFPVTIKIV YDKDSRRILG 400
    AQMAAREDMS MGIHMFSLAI QEGVTIEKLA LTDIFFLPHF NKPYNYITMA 450
    ALGAKD 456
    Length:456
    Mass (Da):49,636
    Last modified:November 23, 2004 - v1
    Checksum:i782106FDC8945744
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti177 – 1771E → V AA sequence 1 PublicationCurated

    Mass spectrometryi

    Molecular mass is 49603.57 Da from positions 1 - 456. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000003 Genomic DNA. Translation: AAT87003.1.
    RefSeqiYP_060186.1. NC_006086.1.

    Genome annotation databases

    EnsemblBacteriaiAAT87003; AAT87003; M6_Spy0868.
    GeneIDi2941962.
    KEGGispa:M6_Spy0868.
    PATRICi19723904. VBIStrPyo30273_0897.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000003 Genomic DNA. Translation: AAT87003.1 .
    RefSeqi YP_060186.1. NC_006086.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2BC0 X-ray 2.00 A/B 2-456 [» ]
    2BC1 X-ray 2.15 A/B 2-456 [» ]
    2BCP X-ray 2.10 A/B 2-456 [» ]
    ProteinModelPortali Q5XC60.
    SMRi Q5XC60. Positions 2-456.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 286636.M6_Spy0868.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAT87003 ; AAT87003 ; M6_Spy0868 .
    GeneIDi 2941962.
    KEGGi spa:M6_Spy0868.
    PATRICi 19723904. VBIStrPyo30273_0897.

    Phylogenomic databases

    eggNOGi COG0446.
    HOGENOMi HOG000276710.
    KOi K00359.
    OMAi HAYIPLA.
    OrthoDBi EOG6QVRCJ.

    Enzyme and pathway databases

    BioCyci SPYO286636:GHNO-928-MONOMER.

    Family and domain databases

    Gene3Di 3.30.390.30. 1 hit.
    InterProi IPR016156. FAD/NAD-linked_Rdtase_dimer.
    IPR013027. FAD_pyr_nucl-diS_OxRdtase.
    IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
    IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
    IPR001327. Pyr_OxRdtase_NAD-bd_dom.
    [Graphical view ]
    Pfami PF00070. Pyr_redox. 1 hit.
    PF07992. Pyr_redox_2. 2 hits.
    PF02852. Pyr_redox_dim. 1 hit.
    [Graphical view ]
    PRINTSi PR00368. FADPNR.
    SUPFAMi SSF55424. SSF55424. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain."
      Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E., Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M.
      J. Infect. Dis. 190:727-738(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-946 / MGAS10394.
    2. "Two-dimensional gel electrophoresis map of Streptococcus pyogenes proteins."
      Hogan D.A., Du P., Stevenson T.I., Whitton M., Kilby G.W., Rogers J., VanBogelen R.A.
      Submitted (MAY-2000) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 75-89; 107-121; 127-143; 163-181 AND 303-315, MASS SPECTROMETRY.
      Strain: JRS4 / Serotype M61 Publication.

    Entry informationi

    Entry nameiNAOX_STRP6
    AccessioniPrimary (citable) accession number: Q5XC60
    Secondary accession number(s): P82571
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The active site is the redox-active Cys-44 oxidized to Cys-SOH. The oxidized form is stabilized by a hydrogen bond formation with His-11 By similarity.By similarity

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3