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Q5XC60 (NAOX_STRP6) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable NADH oxidase
Short name=NOXase
EC=1.6.99.3
Gene names
Ordered Locus Names:M6_Spy0868
OrganismStreptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394) [Complete proteome] [HAMAP]
Taxonomic identifier286636 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus ›

Protein attributes

Sequence length456 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the four-electron reduction of molecular oxygen to water By similarity. UniProtKB P37061

Catalytic activity

NADH + acceptor = NAD+ + reduced acceptor. UniProtKB P37061

Cofactor

Binds 1 FAD per subunit By similarity. UniProtKB P37062

Miscellaneous

The active site is the redox-active Cys-44 oxidized to Cys-SOH. The oxidized form is stabilized by a hydrogen bond formation with His-11 By similarity. UniProtKB P37061

Sequence similarities

Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family.

Mass spectrometry

Molecular mass is 49603.57 Da from positions 1 - 456. Determined by ESI. Ref.2

Ontologies

Keywords
   DomainRedox-active center
   LigandFAD
Flavoprotein
NAD
   Molecular functionOxidoreductase
   PTMOxidation
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological_processcell redox homeostasis

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: InterPro

   Molecular_functionNADH dehydrogenase activity

Inferred from electronic annotation. Source: EC

flavin adenine dinucleotide binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 456456Probable NADH oxidase
PRO_0000259672

Regions

Nucleotide binding8 – 125FAD By similarity UniProtKB P37062
Nucleotide binding110 – 1134FAD By similarity UniProtKB P37062
Nucleotide binding163 – 17816NAD By similarity UniProtKB P37061
Nucleotide binding282 – 29211FAD By similarity UniProtKB P37061

Sites

Active site111Proton acceptor By similarity UniProtKB P37062
Active site441Redox-active By similarity UniProtKB P37061
Binding site441FAD By similarity UniProtKB P37062
Binding site1711NAD; via amide nitrogen By similarity UniProtKB P37062
Binding site1901NAD By similarity UniProtKB P37062
Binding site1991NAD By similarity UniProtKB P37062
Binding site2541NAD; via amide nitrogen By similarity UniProtKB P37062
Binding site2921FAD By similarity UniProtKB P37062
Binding site3081NAD; via carbonyl oxygen By similarity UniProtKB P37062
Binding site3101FAD; via amide nitrogen By similarity UniProtKB P37062
Binding site3391NAD; via carbonyl oxygen By similarity UniProtKB P37062

Amino acid modifications

Modified residue441Cysteine sulfenic acid (-SOH) By similarity UniProtKB P37061

Experimental info

Sequence conflict1771E → V AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q5XC60 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 782106FDC8945744

FASTA45649,636
        10         20         30         40         50         60 
MSKIVVVGAN HAGTACIKTM LTNYGDANEI VVFDQNSNIS FLGCGMALWI GEQIAGPEGL 

        70         80         90        100        110        120 
FYSDKEELES LGAKVYMESP VQSIDYDAKT VTALVDGKNH VETYDKLIFA TGSQPILPPI 

       130        140        150        160        170        180 
KGAEIKEGSL EFEATLENLQ FVKLYQNSAD VIAKLENKDI KRVAVVGAGY IGVELAEAFQ 

       190        200        210        220        230        240 
RKGKEVVLID VVDTCLAGYY DRDLTDLMAK NMEEHGIQLA FGETVKEVAG NGKVEKIITD 

       250        260        270        280        290        300 
KNEYDVDMVI LAVGFRPNTT LGNGKIDLFR NGAFLVNKRQ ETSIPGVYAI GDCATIYDNA 

       310        320        330        340        350        360 
TRDTNYIALA SNAVRTGIVA AHNACGTDLE GIGVQGSNGI SIYGLHMVST GLTLEKAKRL 

       370        380        390        400        410        420 
GFDAAVTEYT DNQKPEFIEH GNFPVTIKIV YDKDSRRILG AQMAAREDMS MGIHMFSLAI 

       430        440        450 
QEGVTIEKLA LTDIFFLPHF NKPYNYITMA ALGAKD

« Hide

References

« Hide 'large scale' references
[1]"Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain."
Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E., Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M.
J. Infect. Dis. 190:727-738(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-946 / MGAS10394.
[2]"Two-dimensional gel electrophoresis map of Streptococcus pyogenes proteins."
Hogan D.A., Du P., Stevenson T.I., Whitton M., Kilby G.W., Rogers J., VanBogelen R.A.
Submitted (MAY-2000) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 75-89; 107-121; 127-143; 163-181 AND 303-315, MASS SPECTROMETRY.
Strain: JRS4 / Serotype M6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000003 Genomic DNA. Translation: AAT87003.1.
RefSeqYP_060186.1. NC_006086.1.

3D structure databases

ProteinModelPortalQ5XC60.
SMRQ5XC60. Positions 2-456.
ModBaseSearch...

Protein-protein interaction databases

STRING286636.M6_Spy0868.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT87003; AAT87003; M6_Spy0868.
GeneID2941962.
KEGGspa:M6_Spy0868.
PATRIC19723904. VBIStrPyo30273_0897.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0446.
HOGENOMHOG000276710.
KOK00359.
OMAEDMSMGI.
ProtClustDBCLSK877163.

Enzyme and pathway databases

BioCycSPYO286636:GHNO-928-MONOMER.

Family and domain databases

Gene3D3.30.390.30. 1 hit.
InterProIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 2 hits.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSPR00368. FADPNR.
SUPFAMSSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNAOX_STRP6
AccessionPrimary (citable) accession number: Q5XC60
Secondary accession number(s): P82571
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 23, 2004
Last modified: May 29, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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