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Q5XC60

- NAOX_STRP6

UniProt

Q5XC60 - NAOX_STRP6

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Protein
Probable NADH oxidase
Gene
M6_Spy0868
Organism
Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the four-electron reduction of molecular oxygen to water By similarity.By similarity

Catalytic activityi

NADH + acceptor = NAD+ + reduced acceptor.By similarity

Cofactori

Binds 1 FAD per subunit By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei11 – 111Proton acceptor By similarityBy similarity
Active sitei44 – 441Redox-active By similarityBy similarity
Binding sitei44 – 441FAD By similarityBy similarity
Binding sitei171 – 1711NAD; via amide nitrogen By similarityBy similarity
Binding sitei190 – 1901NAD By similarityBy similarity
Binding sitei199 – 1991NAD By similarityBy similarity
Binding sitei254 – 2541NAD; via amide nitrogen By similarityBy similarity
Binding sitei292 – 2921FAD By similarityBy similarity
Binding sitei308 – 3081NAD; via carbonyl oxygen By similarityBy similarity
Binding sitei310 – 3101FAD; via amide nitrogen By similarityBy similarity
Binding sitei339 – 3391NAD; via carbonyl oxygen By similarityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 125FAD By similarityBy similarity
Nucleotide bindingi110 – 1134FAD By similarityBy similarity
Nucleotide bindingi163 – 17816NAD By similarityBy similarity
Add
BLAST
Nucleotide bindingi282 – 29211FAD By similarityBy similarity
Add
BLAST

GO - Molecular functioni

  1. NADH dehydrogenase activity Source: UniProtKB-EC
  2. flavin adenine dinucleotide binding Source: InterPro

GO - Biological processi

  1. cell redox homeostasis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciSPYO286636:GHNO-928-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable NADH oxidase (EC:1.6.99.3)
Short name:
NOXase
Gene namesi
Ordered Locus Names:M6_Spy0868
OrganismiStreptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Taxonomic identifieri286636 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000001167: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 456456Probable NADH oxidase
PRO_0000259672Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei44 – 441Cysteine sulfenic acid (-SOH) By similarityBy similarity

Keywords - PTMi

Oxidation

Interactioni

Protein-protein interaction databases

STRINGi286636.M6_Spy0868.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 75
Helixi11 – 2414
Helixi25 – 273
Beta strandi28 – 336
Beta strandi35 – 373
Helixi43 – 453
Helixi46 – 505
Beta strandi53 – 553
Helixi58 – 603
Helixi65 – 706
Beta strandi74 – 763
Beta strandi81 – 855
Turni86 – 894
Beta strandi90 – 956
Beta strandi98 – 1036
Beta strandi105 – 1095
Beta strandi113 – 1153
Beta strandi134 – 1363
Beta strandi139 – 1413
Helixi145 – 15410
Beta strandi162 – 1665
Helixi170 – 18112
Beta strandi185 – 19410
Turni195 – 1995
Helixi202 – 21312
Turni214 – 2163
Beta strandi218 – 2214
Beta strandi225 – 2295
Beta strandi231 – 2333
Beta strandi236 – 2416
Beta strandi243 – 2453
Beta strandi247 – 2515
Beta strandi255 – 2573
Helixi260 – 2623
Beta strandi287 – 2893
Helixi291 – 2933
Beta strandi296 – 2983
Turni299 – 3024
Beta strandi303 – 3053
Helixi310 – 32415
Beta strandi338 – 3425
Beta strandi345 – 3517
Helixi354 – 3596
Beta strandi364 – 37411
Beta strandi384 – 3929
Turni393 – 3953
Beta strandi397 – 40711
Helixi412 – 42211
Helixi426 – 4316
Turni438 – 4403
Helixi446 – 4527

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BC0X-ray2.00A/B2-456[»]
2BC1X-ray2.15A/B2-456[»]
2BCPX-ray2.10A/B2-456[»]
ProteinModelPortaliQ5XC60.
SMRiQ5XC60. Positions 2-456.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiCOG0446.
HOGENOMiHOG000276710.
KOiK00359.
OMAiHAYIPLA.
OrthoDBiEOG6QVRCJ.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
InterProiIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamiPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 2 hits.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSiPR00368. FADPNR.
SUPFAMiSSF55424. SSF55424. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5XC60-1 [UniParc]FASTAAdd to Basket

« Hide

MSKIVVVGAN HAGTACIKTM LTNYGDANEI VVFDQNSNIS FLGCGMALWI    50
GEQIAGPEGL FYSDKEELES LGAKVYMESP VQSIDYDAKT VTALVDGKNH 100
VETYDKLIFA TGSQPILPPI KGAEIKEGSL EFEATLENLQ FVKLYQNSAD 150
VIAKLENKDI KRVAVVGAGY IGVELAEAFQ RKGKEVVLID VVDTCLAGYY 200
DRDLTDLMAK NMEEHGIQLA FGETVKEVAG NGKVEKIITD KNEYDVDMVI 250
LAVGFRPNTT LGNGKIDLFR NGAFLVNKRQ ETSIPGVYAI GDCATIYDNA 300
TRDTNYIALA SNAVRTGIVA AHNACGTDLE GIGVQGSNGI SIYGLHMVST 350
GLTLEKAKRL GFDAAVTEYT DNQKPEFIEH GNFPVTIKIV YDKDSRRILG 400
AQMAAREDMS MGIHMFSLAI QEGVTIEKLA LTDIFFLPHF NKPYNYITMA 450
ALGAKD 456
Length:456
Mass (Da):49,636
Last modified:November 23, 2004 - v1
Checksum:i782106FDC8945744
GO

Mass spectrometryi

Molecular mass is 49603.57 Da from positions 1 - 456. Determined by ESI. 1 Publication

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti177 – 1771E → V AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000003 Genomic DNA. Translation: AAT87003.1.
RefSeqiYP_060186.1. NC_006086.1.

Genome annotation databases

EnsemblBacteriaiAAT87003; AAT87003; M6_Spy0868.
GeneIDi2941962.
KEGGispa:M6_Spy0868.
PATRICi19723904. VBIStrPyo30273_0897.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000003 Genomic DNA. Translation: AAT87003.1 .
RefSeqi YP_060186.1. NC_006086.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2BC0 X-ray 2.00 A/B 2-456 [» ]
2BC1 X-ray 2.15 A/B 2-456 [» ]
2BCP X-ray 2.10 A/B 2-456 [» ]
ProteinModelPortali Q5XC60.
SMRi Q5XC60. Positions 2-456.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 286636.M6_Spy0868.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAT87003 ; AAT87003 ; M6_Spy0868 .
GeneIDi 2941962.
KEGGi spa:M6_Spy0868.
PATRICi 19723904. VBIStrPyo30273_0897.

Phylogenomic databases

eggNOGi COG0446.
HOGENOMi HOG000276710.
KOi K00359.
OMAi HAYIPLA.
OrthoDBi EOG6QVRCJ.

Enzyme and pathway databases

BioCyci SPYO286636:GHNO-928-MONOMER.

Family and domain databases

Gene3Di 3.30.390.30. 1 hit.
InterProi IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view ]
Pfami PF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 2 hits.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view ]
PRINTSi PR00368. FADPNR.
SUPFAMi SSF55424. SSF55424. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Progress toward characterization of the group A Streptococcus metagenome: complete genome sequence of a macrolide-resistant serotype M6 strain."
    Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E., Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M.
    J. Infect. Dis. 190:727-738(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-946 / MGAS10394.
  2. "Two-dimensional gel electrophoresis map of Streptococcus pyogenes proteins."
    Hogan D.A., Du P., Stevenson T.I., Whitton M., Kilby G.W., Rogers J., VanBogelen R.A.
    Submitted (MAY-2000) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 75-89; 107-121; 127-143; 163-181 AND 303-315, MASS SPECTROMETRY.
    Strain: JRS4 / Serotype M6.

Entry informationi

Entry nameiNAOX_STRP6
AccessioniPrimary (citable) accession number: Q5XC60
Secondary accession number(s): P82571
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 23, 2004
Last modified: May 14, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The active site is the redox-active Cys-44 oxidized to Cys-SOH. The oxidized form is stabilized by a hydrogen bond formation with His-11 By similarity.By similarity

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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